Consulta: creatorFacets:"Cassone, Anne-laure"
Registros recuperados: 6
Data/hora: 06/06/2024 06:12:49

Exposure of marine mussels Mytilus spp. to polystyrene microplastics: Toxicity and influence on fluoranthene bioaccumulation
Provedor de dados: 5
Autores: Paul-pont, Ika; Lacroix, Camille; Gonzalez Fernandez, Carmen; Hegaret, Helene; Lambert, Christophe; Le Goic, Nelly; Frere, Laura; Cassone, Anne-laure; Sussarellu, Rossana; Fabioux, Caroline; Guyomarch, Julien; Albentosa, Marina; Huvet, Arnaud; Soudant, Philippe.
The effects of polystyrene microbeads (micro-PS; mix of 2 and 6 μm; final concentration: 32 μg L−1) alone or in combination with fluoranthene (30 μg L−1) on marine mussels Mytilus spp. were investigated after 7 days of exposure and 7 days of depuration under controlled laboratory conditions. Overall, fluoranthene was mostly associated to algae Chaetoceros muelleri (partition coefficient Log Kp = 4.8) used as a food source for mussels during the experiment. When micro-PS were added in the system, a fraction of FLU transferred from the algae to the microbeads as suggested by the higher partition coefficient of micro-PS (Log Kp = 6.6), which confirmed a high affinity of fluoranthene for polystyrene microparticles. However, this did not lead to a modification...
Tipo: Text
Palavras-chave: Microplastics; Fluoranthene; Mussel; Depuration; Oxidative system.
Ano: 2016
URL: https://archimer.ifremer.fr/doc/00344/45502/45848.pdf

Low incidence of clonality in cold water corals revealed through the novel use of standardized protocol adapted to deep sea sampling
Provedor de dados: 5
Autores: Becheler, Ronan; Cassone, Anne-laure; Noel, Philippe; Mouchel, Olivier; Morrison, Cheryl L.; Arnaud-haond, Sophie.
Sampling in the deep sea is a technical challenge, which has hindered the acquisition of robust datasets that are necessary to determine the fine-grained biological patterns and processes that may shape genetic diversity. Estimates of the extent of clonality in deep-sea species, despite the importance of clonality in shaping the local dynamics and evolutionary trajectories, have been largely obscured by such limitations. Cold-water coral reefs along European margins are formed mainly by two reef-building species, Lophelia pertusa and Madrepora oculata. Here we present a fine-grained analysis of the genotypic and genetic composition of reefs occurring in the Bay of Biscay, based on an innovative deep-sea sampling protocol. This strategy was designed to be...
Tipo: Text
Palavras-chave: Cold-water coral; Lophelia pertusa; Madrepora oculata; Clonality; Fine-grained spatial genetic structure; Standardized sampling.
Ano: 2017
URL: https://archimer.ifremer.fr/doc/00300/41117/40291.pdf

Toxic effects of leachates from plastic pearl-farming gear on embryo-larval development in the pearl oyster Pinctada margaritifera
Provedor de dados: 5
Autores: Gardon, Tony; Huvet, Arnaud; Paul-pont, Ika; Cassone, Anne-laure; Koua, Manaarii Sham; Soyez, Claude; Jezequel, Ronan; Receveur, Justine; Le Moullac, Gilles.
Pearl-farming leads to significant plastic pollution in French Polynesia (FP) as the end of life of most farming gear is currently poorly managed. Plastic debris released into the aquatic environment accumulates, with potentially detrimental effects on the lagoon ecosystem and pearl oyster Pinctada margaritifera, a species of ecological, commercial and social value. Here, we tested the effects of leachates from new (N) and aged (A) plastic pearl-farming gear (spat collector and synthetic rope) obtained after 24 h and 120 h incubation, on the embryo-larval development of the pearl oyster using an in-vitro assay. Embryos were exposed for 24 h and 48 h to a negative control (0) and the leachate from 0.1, 1, 10 and 100 g of plastic. L−1. After 24 h exposure to...
Tipo: Text
Palavras-chave: Aquaculture; Synthetic materials; Hazardous chemicals; Toxicity; Marine bivalve; Early development.
Ano: 2020
URL: https://archimer.ifremer.fr/doc/00627/73917/73257.pdf

Microplastics in seafood: Benchmark protocol for their extraction and characterization
Provedor de dados: 5
Autores: Dehaut, Alexandre; Cassone, Anne-laure; Frere, Laura; Hermabessiere, Ludovic; Himber, Charlotte; Rinnert, Emmanuel; Riviere, Gilles; Lambert, Christophe; Soudant, Philippe; Huvet, Arnaud; Duflos, Guillaume; Paul-pont, Ika.
Pollution of the oceans by microplastics (&lt;5 mm) represents a major environmental problem. To date, a limited number of studies have investigated the level of contamination of marine organisms collected in situ. For extraction and characterization of microplastics in biological samples, the crucial step is the identification of solvent(s) or chemical(s) that efficiently dissolve organic matter without degrading plastic polymers for their identification in a time and cost effective way. Most published papers, as well as OSPAR recommendations for the development of a common monitoring protocol for plastic particles in fish and shellfish at the European level, use protocols containing nitric acid to digest the biological tissues, despite reports of...
Tipo: Text
Palavras-chave: Microplastics; Digestion; Method; Seafood products; Tissue; Plastic integrity.
Ano: 2016
URL: https://archimer.ifremer.fr/doc/00335/44582/44469.pdf

First description of French V. tubiashii strains pathogenic to mollusk: I. Characterization of isolates and detection during mortality events
Provedor de dados: 5
Autores: Travers, Marie-agnes; Mersni Achour, Rachida; Haffner, Philippe; Tourbiez, Delphine; Cassone, Anne-laure; Morga, Benjamin; Doghri, Ibtissem; Garcia, Celine; Renault, Tristan; Fruitier-arnaudin, Ingrid; Saulnier, Denis.
Nine dominant bacterial isolates were obtained from different batches of Crassostrea gigas spat experiencing high mortality rates in a French experimental hatchery/nursery in 2007. Using phenotypic analysis combined with multilocus sequence analysis, the isolates were shown to be genetically close to the Vibrio tubiashii type strain. Based on (1) analyses of the recA gene sequences; (2) the results of DNA–DNA hybridization assays between 07/118 T2 (LMG 27884 = CECT 8426), which is a representative strain, and the V. tubiashii type strain (69%); and (3) phenotypic traits, the bacteria were classified in a group close to American V. tubiashii strain. Its virulence (70% of mortalities) and the toxicity of the extracellular products of 07/118 T2 was...
Tipo: Text
Palavras-chave: Crassostrea gigas Pathogenicity Extracellular products Polyphasic approach; Real time PCR.
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00189/30028/28743.pdf

Microplastic bacterial communities in the Bay of Brest: Influence of polymer type and size
Provedor de dados: 5
Autores: Frere, Laura; Maignien, Lois; Chalopin, Morgane; Huvet, Arnaud; Rinnert, Emmanuel; Morrison, Hilary; Kerninon, Sandrine; Cassone, Anne-laure; Lambert, Christophe; Reveillaud, Julie; Paul-pont, Ika.
Microplastics (&lt;5 mm) exhibit intrinsic features such as density, hydrophobic surface, or high surface/volume ratio, that are known to promote microbial colonization and biofilm formation in marine ecosystems. Yet, a relatively low number of studies have investigated the nature of microplastic associated bacterial communities in coastal ecosystems and the potential factors influencing their composition and structure. Here, we characterized microplastics collected in the Bay of Brest by manual sorting followed by Raman spectroscopy and studied their associated bacterial assemblages using 16S amplicon high-throughput sequencing. Our methodology allowed discriminating polymer type (polyethylene, polypropylene and polystyrene) within small size ranges...
Tipo: Text
Palavras-chave: Bacteria; Microplastics; Coastal ecosystem; Metabarcoding; Vibrios.
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00449/56082/57614.pdf