Consulta: creatorFacets:"Castagnaro,Atilio Pedro"
Registros recuperados: 5
Data/hora: 06/06/2024 14:11:40

Use of AFLP markers to estimate molecular diversity of Phakopsora pachyrhizi
Provedor de dados: 69
Autores: Lourdes Rocha,Carla Maria; Vellicce,Gabriel Ricardo; García,María Gabriela; Pardo,Esteban Mariano; Racedo,Josefina; Perera,María Francisca; de Lucía,Adrian; Gilli,Javier; Bogado,Noelia; Bonnecarrère,Victoria; German,Silvia; Marcelino,Francismar; Ledesma,Fernando; Reznikov,Sebastián; Ploper,Leonardo Daniel; Welin,Bjorn; Castagnaro,Atilio Pedro.
Background Asian soybean rust (SBR) caused by Phakopsora pachyrhizi Syd. &amp;amp; Syd., is one of the main diseases affecting soybean and has been reported as one of the most economically important fungal pathogens worldwide. Knowledge of the genetic diversity of this fungus should be considered when developing resistance breeding strategies. We aimed to analyze the genetic diversity of P. pachyrhizi combining simple sampling with a powerful and reproducible molecular technique. Results We employed Amplified Fragment Length Polymorphism (AFLP) technique for the amplification of P. pachyrhizi DNA extracted from naturally SBR-infected plants from 23 production fields. From a total of 1919 markers obtained, 77% were polymorphic. The high percentage of...
Tipo: Journal article
Palavras-chave: Asian soybean rust; Genetic variation; Glycine max; Molecular markers.
Ano: 2015
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000600009

Biotecnología para el desarrollo y sostenibilidad agroindustrial del Noroeste Argentino
Provedor de dados: 46
Autores: Castagnaro,Atilio Pedro; Filippone,María Paula; Noguera,Aldo Sergio; Perera,María Francisca; Vellicce,Gabriel Ricardo; Mamaní,Alicia Inés; Ontivero,Marta Inés; Arias,Marta Eugenia; Racedo,Josefina; García,María Gabriela; Paz,Nora; Díaz,María Elena; Sendín,Lorena Noelia; Pardo,Esteban Mariano; Orce,Ingrid Georgina; Rocha,Carla María Lourdes; Sepúlveda Tusek,Mlena; Martínez Zamora,Gustavo; Salazar,Sergio Miguel; Dantur,Karina Inés; Rigano,Luciano; Torres,Pablo; Chalfoun,Nadia Regina; Maraño,María Rosa; Vojnov,Adrián; Ploper,Leonardo Daniel; Díaz Ricci,Juan Carlos.
En 1994 se conformó un grupo de investigación científica y desarrollo tecnológico guiado por la demanda del sector agroindustrial de la región del noroeste argentino, que además de las consideraciones económicas intrínsecas del proceso productivo, tuvo en cuenta para sus investigaciones, aspectos sociales y ambientales. Las premisas conceptuales del trabajo científico fueron el estudio, la preservación y el aprovechamiento de la biodiversidad, en conjunción con la búsqueda continua de alternativas de manejo fitosanitario lo menos reñidas posible con la salud humana y ambiental. Fue así que en 1997 se inició el primer Programa Nacional de Mejoramiento Genético de la Frutilla en Argentina, que desde el INSIBIO (CONICET-UNT) en Tucumán coordinó el trabajo de...
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Bioproductos; Caña de azúcar; Citrus; Cultivo de tejidos y micropropagación; EEAOC; Frutilla; INSIBIO; Marcadores moleculares; Mejoramiento genético molecular; Soja; Transformación de plantas.
Ano: 2011
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1852-62332011000100016

An overview of sugarcane brown rust in Cuba
Provedor de dados: 63
Autores: La O,María; Perera,María Francisca; Bertani,Romina Priscila; Acevedo,Ricardo; Arias,Marta Eugenia; Casas,Mario Alberto; Pérez,Juana; Puchades,Yaquelín; Rodríguez,Eida; Alfonso,Isabel; Castagnaro,Atilio Pedro.
ABSTRACT: Multiple pathogens affect sugarcane, among them Puccinia melanocephala, the causal agent of brown rust. This disease was first reported in Cuba in 1979 when it was responsible for a severe attack on the main sugarcane variety B4362. The aims of the present study were to give an overview of sugarcane brown rust in Cuba and show the current disease situation in the country. A retrospective analysis regard to sugarcane cultivar composition resistant to brown rust in Cuba was carried out. In addition 154 genotypes, including the most used progenitors in the breeding program and commercial varieties were evaluated under natural infection conditions. The identity of P. melanocephala was verified by Polymerase Chain Reaction (PCR) and by sequencing the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Puccinia melanocephala; Varietal mosaic; Resistance.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162018000300233

Novel set of real-time PCR primers for simultaneous detection of Liberibacter species associated with citrus Huanglongbing
Provedor de dados: 63
Autores: Orce,Ingrid Georgina; Sendín,Lorena Noelia; Marano,María Rosa; Vojnov,Adrián Alberto; Castagnaro,Atilio Pedro; Filippone,María Paula.
Huanglongbing (HLB), a devastating citrus disease caused by the bacterium “Candidatus Liberibacter spp.”, is now responsible for significant economic losses worldwide. Yet, no effective disease control has been found, and the non-cultivability of the bacterium has severely hampered studies on the pathogen. The 16S rDNA gene is a well-characterized sequence, essential for cell survival, and is used for bacterial identification or assignment of close relationships at the genus and species levels. Quantitative Real-Time PCR (qPCR) assays based on 16S rDNA genes are widely used in the detection of “Ca. Liberibacter spp.” in multiplex reactions. We have developed for the first time a set of qPCR primers based on the conserved 16S rDNA gene, which specifically...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Candidatus Liberibacter spp.; Syber Green qPCR; Argentina; Greening; Pathogen detection.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162015000300252

Morphological and molecular characterization of Puccinia kuehnii, the causal agent of sugarcane orange rust, in Cub
Provedor de dados: 63
Autores: Perera,María Francisca; Bertani,Romina Priscila; Arias,Marta Eugenia; Hechavarría,María de la Luz La O; Navarro,María de los Ángeles Zardón; Debes,Mario Alberto; Luque,Ana Catalina; Cuenya,María Inés; Rojas,Ricardo Acevedo; Castagnaro,Atilio Pedro.
ABSTRACT Sugarcane orange rust caused by Puccinia kuehnii has recently become an important disease in sugarcane crops and its spread is causing great concern to growers. In this study, we analyzed spores from symptomatic orange rust sugarcane leaves collected in multiple locations in Cuba in a 4-year-period in order to characterize morphological traits of P. kuehnii, establish an adequate molecular technique to characterize it, and determine its infection court in sugarcane. Orange rust caused by P. kuehnii was confirmed by polymerase chain reaction (PCR) and morphological characterization. AFLP markers detected high diversity in P. kuenhnii samples. Sequencing of rDNA regions, as expected, did not reveal differences and SSR markers designed for P....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: AFLP; RDNA; SSR; Infection courts.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162020000201200