Consulta: creatorFacets:"Castro,Antonio Fernando Pestana de"
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Data/hora: 06/06/2024 02:51:45

Adhesive properties of an outer structure of Clostridium perfringens type A isolated from piglets with with catarrhal enteritis
Provedor de dados: 59
Autores: Teixeira,Elizabeth Pelosi; Serafim,Marlene Braide; Höfling,Maria Alice Cruz; Yamada,Aureo T.; Castro,Antonio Fernando Pestana de.
One strain (S32) of Clostridium perfringens type A was isolated from a case of catarrhal enteritis of piglets. This strain was able to adhere to HeLa cells showing an adherence index (AI) of 25.15 ± 1.26 (mean ± 1 standard error of the mean). Treatment of the bacterial cells with trypsin (0.25mg/ml) decreased in 70%-80% the AI and metaperiodate (10mg/ml) abolished completely the adherence, suggesting that the structure responsible for this phenomenon was probably a glycoprotein. Heating of bacterial suspensions (100ºC/5 min) before carrying out the adhesion test decreased the AI rendering it equal to the negative controls. Rabbit homologous S32 antiserum inhibited the adherence up to dilutions of 1: 640, at least. The piglet ileal loop assay, carried out...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cl. perfringens type A; Adherence; Fibrillar structure; Enteritis; Piglets.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141999000300010

Biological and molecular characterization of Streptococcus crista strains isolated from human dental biofilm by means of arbitrary primers - PCR (AP-PCR)
Provedor de dados: 58
Autores: Angelini,Michelle; Silveira,Wanderley Dias da; Campos,Tatiana Amabile de; Cai,Silvana; Castro,Antonio Fernando Pestana de.
Streptococcus ssp are important components of the dental biofilm and Streptococcus crista is considered to be an interesting model of bacterial interactions taking place in this biofilm. In the present work, S. crista strains were isolated from the dental biofilm of Brazilian individuals and studied with respect to their biological characteristics and their molecular profile by means of AP-PCR techniques, using the RR2, 434, OPR2, OPR8, and OPR13 primers. Results allowed us to build a similarity dendrogram. Analysis of the similarity dendrogram allowed the separation of the studied strains into similarity groups. All isolates presented fibril tufts by Transmission Electron Microscopy (TEM). These isolates were able to bind to salivary amylase and to adhere...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biological characterization; Molecular characterization; Dendrogram analysis; Biofilm.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000200005

IDENTIFICAÇÃO DOS GENES QUE CODIFICAM PARA A ENTEROTOXINA TERMOLÁBIL LT-II EM AMOSTRAS DE ESCHERICHIA COLI ISOLADAS DE BEZERROS COM DIARRÉIA NA REGIÃO DE JABOTICABAL, SP, BRASIL
Provedor de dados: 65
Autores: Ugrinovich,Leila Aidar; De Ávila,Fernando Antonio; Oliveira,Maria Natália; Castro,Antonio Fernando Pestana de.
Examinando 52 espécimes fecais de bezerros com diarréia de fazendas da região de Jaboticabal, SP, Brasil, uma das amostras de Escherichia coli isoladas, quando analisada pela Reação em Cadeia da Polimerase (PCR), demonstrou a presença dos genes que codificam para a enterotoxina termolábil do tipo LT-II. Este é o primeiro relato de amostra de E. coli enterotoxigênica, isolada de bezerros com diarréia no Brasil, contendo os genes para codificação da enterotoxina LT-II. Encontram-se apenas citações de isolamento no Brasil de amostras de E. coli LT-II+ de alimentos de origem animal.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: E.coli; Enterotoxina LT-II; Detecção do gene; PCR; Brasil.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782002000200017