Consulta: creatorFacets:"Castrucci,A.M.L."
Registros recuperados: 5
Data/hora: 06/06/2024 03:37:59

Biological activity and binding of estradiol to SK-Mel 23 human melanoma cells
Provedor de dados: 56
Autores: Sarti,M.S.M.V.; Visconti,M.A.; Castrucci,A.M.L.
Patients expressing estradiol receptors in melanoma cells have been reported to have a better prognosis. We therefore decided to investigate the in vitro effects of ß-estradiol and tamoxifen on the growth and tyrosinase activity of SK-Mel 23 human melanoma cells. Twenty-four-hour treatment with 0.4 nM ß-estradiol inhibited cell proliferation in 30% (0.70 ± 0.03 x 10(5) cells) and increased tyrosinase activity in 50% (7130.5 ± 376.5 cpm/10(5) cells), as compared to untreated cells (1.0 ± 0.05 x 10(5) cells and 4769 ± 25.5 cpm/10(5) cells, respectively). Both responses were completely (100%) blocked by 1 µM tamoxifen. Higher concentrations (up to 1.6 nM) or longer treatments (up to 72 h) did not result in a larger effect of the hormone on proliferation or...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: SK-Mel 23 cell line; Human melanoma; SS-estradiol; Tamoxifen; Cell proliferation; Tyrosinase activity.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000600016

Modulation of rhodopsin gene expression and signaling mechanisms evoked by endothelins in goldfish and murine pigment cell lines
Provedor de dados: 56
Autores: Lopes,G.J.D.; Góis,C.C.; Lima,L.H.R.G.; Castrucci,A.M.L.
Endothelins (ETs) and sarafotoxins (SRTXs) belong to a family of vasoconstrictor peptides, which regulate pigment migration and/or production in vertebrate pigment cells. The teleost Carassius auratus erythrophoroma cell line, GEM-81, and Mus musculus B16 melanocytes express rhodopsin, as well as the ET receptors, ETB and ETA, respectively. Both cell lines are photoresponsive, and respond to light with a decreased proliferation rate. For B16, the doubling time of cells kept in 14-h light (14L):10-h darkness (10D) was higher compared to 10L:14D, or to DD. The doubling time of cells kept in 10L:14D was also higher compared to DD. Using real-time PCR, we demonstrated that SRTX S6c (12-h treatment, 100 pM and 1 nM; 24-h treatment, 1 nM) and ET-1 (12-h...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rhodopsin; Sarafotoxin; Endothelin; Pigment cells; Gene expression; Signaling pathway.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000900004

The expression of melanopsin and clock genes in Xenopus laevis melanophores and their modulation by melatonin
Provedor de dados: 56
Autores: Bluhm,A.P.C.; Obeid,N.N.; Castrucci,A.M.L.; Visconti,M.A.
Vertebrates have a central clock and also several peripheral clocks. Light responses might result from the integration of light signals by these clocks. The dermal melanophores of Xenopus laevis have a photoreceptor molecule denominated melanopsin (OPN4x). The mechanisms of the circadian clock involve positive and negative feedback. We hypothesize that these dermal melanophores also present peripheral clock characteristics. Using quantitative PCR, we analyzed the pattern of temporal expression of Opn4x and the clock genes Per1, Per2, Bmal1, and Clock in these cells, subjected to a 14-h light:10-h dark (14L:10D) regime or constant darkness (DD). Also, in view of the physiological role of melatonin in the dermal melanophores of X. laevis, we determined...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Xenopus laevis; Dermal melanophores; Melatonin; Melanopsin; Per1; Per2; Bmal1; Clock.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000800007

Topical application of a melanotropin analogue to vulgar vitiligo dermo-epidermal minigrafts
Provedor de dados: 56
Autores: Schwartzmann-Solon,A.M.; Visconti,M.A.; Castrucci,A.M.L.
Human subjects with active vulgar vitiligo do not respond well to autologous dermo-epidermal minigrafting. Eighteen subjects were treated with the &lt;FONT FACE="Symbol"&gt;a&lt;/FONT&gt;-melanocyte-stimulating hormone (&lt;FONT FACE="Symbol"&gt;a&lt;/FONT&gt;-MSH) synthetic analogue &amp;amp;#091;Nle4, D-Phe7&amp;amp;#093;-&lt;FONT FACE="Symbol"&gt;a&lt;/FONT&gt;-MSH. The hormone (50 µl, 0.4 mM) was applied topically to 30-cm2 lesions in which 29-48 minigrafts had been made. The hormone did not improve the success of the minigrafting and no differences were observed in local or distant repigmentation in treated subjects as compared to the placebo group. Aliquots of 24-h urine concentrated by lyophilization irreversibly darkened toad skins, demonstrating...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Vulgar vitiligo &lt; FONT FACE=Symbol&gt; A&lt; /FONT&gt; -MSH synthetic analogue minigrafting.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001200008

The role of calcium, calcium-activated K+ channels, and tyrosine/kinase in psoralen-evoked responses in human melanoma cells
Provedor de dados: 56
Autores: Isoldi,M.C.; Pereira,E.A.; Visconti,M.A.; Castrucci,A.M.L.
8-Methoxy psoralen (8-MOP) exerts a short-term (24 h) mitogenic action, and a long-term (48-72 h) anti-proliferative and melanogenic action on two human melanoma cell lines, SK-Mel 28 and C32TG. An increase of intracellular calcium concentration was observed by spectrofluorometry immediately after the addition of 0.1 mM 8-MOP to both cell lines, previously incubated with calcium probe fluo-3 AM (5 µM). The intracellular Ca2+ chelator BAPTA/AM (1 µM) blocked both early (mitogenic) and late (anti-proliferative and melanogenic) 8-MOP effects on both cell lines, thus revealing the importance of the calcium signal in both short- and long-term 8-MOP-evoked responses. Long-term biological assays with 5 and 10 mM tetraethylammonium chloride (TEA, an inhibitor of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: 8-[Methoxy]-psoralen; SK-Mel 28 cell line; C32TG cell line; Cell proliferation; Cell signaling; Melanoma.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000400013