Consulta: creatorFacets:"Coelho,Lia Alencar"
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Data/hora: 02/06/2024 01:49:31

Melatonin in maturation media fails to improve oocyte maturation, embryo development rates and DNA damage of bovine embryos
Provedor de dados: 63
Autores: Takada,Luciana; Martins Junior,Alicio; Mingoti,Gisele Zoccal; Balieiro,Julio César Carvalho; Coelho,Lia Alencar.
Melatonin (MEL) acts as a powerful scavenger of free radicals and direct gonadal responses to melatonin have been reported in the literature. Few studies, however, have evaluated the effect of MEL during in vitro maturation (IVM) on bovine embryos. This study tested the addition of MEL to maturation medium (MM) with no gonadotropins on nuclear maturation and embryo development rates and the incidence of DNA damage in resulting embryos. Cumulus-oocyte complexes were aspirated from abattoir ovaries and cultured in MM (TCM-199 medium supplemented with 10% fetal calf serum - FCS) at 39ºC and 5% CO2 in air. After 24 hours of culture in MM with 0.5 µg mL-1 FSH and 5.0 µg mL-1 LH; 10-9 M MEL) or 10-9 M MEL, 0.5 µg mL-1 FSH and 5.0 µg mL-1 LH, the oocytes were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Comet assay; Embryos; Gonadotropins; In vitro fertilization; Melatonin.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162010000400003