Consulta: creatorFacets:"Colas, Florent"
Registros recuperados: 27
Data/hora: 09/06/2024 04:49:05

Version évolutive de l’outil opérationnel de numérisation et d’analyse semi-automatique d’images de phytoplancton, utilisant le matériel FlowCAM et le logiciel ZooPhytoImage. Nouvelles perspectives
Provedor de dados: 5
Autores: Wacquet, Guillaume; Grosjean, Philippe; Colas, Florent; Hamad, Denis; Artigas, Luis Felipe.
The coupled system FlowCAM/ZooPhytoImage has become a real operational tool in 2014. However, to be fully adapted to the observations of phytoplankton performed in the context of the REPHY observation network and in order to better respond to present and future requests concerning the evaluation of quality of coastal and marine waters within the European requirements, such as the WFD and MSFD, new functionalities must be integrated into existing tools. Therefore, different axis of development have been proposed by UMONS and Ifremer to adapt both the digitization device and the Zoo/PhytoImage software to the constraints defined by the REPHY. First, version 5 of Zoo/PhytoImage contains recent innovations such as the development of routines to automatically...
Tipo: Text
Palavras-chave: Plancton; Analyse automatisée; Analyse d'image; Classification supervisée; Apprentissage actif; Dénombrement de cellules; Plankton; Automated analysis; Image processing; Supervised classification; Active learning; Cells counting.
Ano: 2015
URL: http://archimer.ifremer.fr/doc/00389/49986/50573.pdf

Toward in situ detection of algae species
Provedor de dados: 5
Autores: Colas, Florent; Laurent, Sebastien; Lehaitre, Michel; Hamelin, Muriel; Crassous, Marie-pierre; Antoine, Elisabeth; Dreanno, Catherine; Deuff, Albert; Compere, Chantal.
For the last few years there is a more and more pressing need to develop system for detecting HAB at their early stage. Researchers have then been working on new in situ sensor. In this paper a submarine transducer based on surface plasmon resonance is reported. It demonstrated a refractive index resolution of 3.10(-6), which is promising for later phytoplankton detection.
Tipo: Text
Ano: 2009
URL: http://archimer.ifremer.fr/doc/00107/21802/20328.pdf

Optimisation de l’identification et du dénombrement du microphytoplancton avec le système couplé de numérisation et d’analyse d’images FlowCAM – Zoo/PhytoImage (système innovant). Action 9 – Livrable 3. Evolution du matériel de numérisation : prototype FastCAM et perspectives. Rapport final, février 2016
Provedor de dados: 5
Autores: Colas, Florent; Tardivel, Morgan; Evrard, Justine; Forest, Bertrand; Crassous, Marie-pierre; Lunven, Michel; Danielou, Marie-madeleine.
This deliverable contains all development work of the FastCAM, new tool for fast imaging of the phytoplankton, which took place in 2015. It consists of: One report of the FastCAM and its comparison with the FlowCAM presentation: The FlowCAM enables the digitalization of a sample of phytoplankton with X10 and X 4 magnifications. The first allows a better morphological description and therefore a higher taxonomic resolution but with a longer scan time (16 X). Thus, for routine analyses, only scanning at 4 X is possible. A considerable gain would be able to do an acquisition to the magnification 10 X with a time of analysis more short, comparable to 4 X. A fast flow imaging system has therefore developed in this sense. He was called FastCAM. The system is...
Tipo: Text
Palavras-chave: FlowCAM; FastCAM; Imagerie rapide; Phytoplancton; REPHY.; FlowCAM; FastCAM; Fast imaging; Phytoplankton; REPHY.
Ano: 2016
URL: http://archimer.ifremer.fr/doc/00363/47420/47425.pdf

Chalcogenide Glass Optical Waveguides for Infrared Biosensing
Provedor de dados: 5
Autores: Anne, Marie-laure; Keirsse, Julie; Nazabal, Virginie; Hyodo, Koji; Inoue, Satoru; Boussard-pledel, Catherine; Lhermite, Herve; Charrier, Joel; Yanakata, Kiyoyuki; Loreal, Olivier; Le Person, Jenny; Colas, Florent; Compere, Chantal; Bureau, Bruno.
Due to the remarkable properties of chalcogenide (Chg) glasses, Chg optical waveguides should play a significant role in the development of optical biosensors. This paper describes the fabrication and properties of chalcogenide fibres and planar waveguides. Using optical fibre transparent in the mid-infrared spectral range we have developed a biosensor that can collect information on whole metabolism alterations, rapidly and in situ. Thanks to this sensor it is possible to collect infrared spectra by remote spectroscopy, by simple contact with the sample. In this way, we tried to determine spectral modifications due, on the one hand, to cerebral metabolism alterations caused by a transient focal ischemia in the rat brain and, in the other hand, starvation...
Tipo: Text
Palavras-chave: Planar waveguide; Fibre; Optical sensor; Chalcogenide.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/2009/publication-6851.pdf

Comparison of adhesion layers of gold on silicate glasses for SERS detection
Provedor de dados: 5
Autores: Colas, Florent; Barchiesi, D.; Kessentini, S.; Toury, T.; De La Chapelle, M. Lamy.
Gold is one of the most widely used metals for building up plasmonic devices. Although slightly less efficient than silver for producing sharp resonance, its chemical properties make it one of the best choices for designing sensors. Sticking gold on a silicate glass substrate requires an adhesion layer, whose effect has to be taken into account. Traditionally, metals (Cr or Ti) or dielectric materials (TiO2 or Cr2O3 ) are deposited between the glass and the nanoparticle. Recently, indium tin oxide and (3-mercaptopropyl)trimethoxysilane (MPTMS) were used as a new adhesion layer. The aim of this work is to compare these six adhesion layers for surface- enhanced Raman scattering sensors by numerical modeling. The near-field and the far-field optical responses...
Tipo: Text
Palavras-chave: Surface-enhanced raman scattering; Adhesion layer; Nanocylinder; Discrete dipole approximation.
Ano: 2015
URL: http://archimer.ifremer.fr/doc/00298/40907/40083.pdf

Toward Detection of Harmful Algae Blooms by in situ Surface Plasmon Resonance Spectroscopy
Provedor de dados: 5
Autores: Laurent, Sebastien; Colas, Florent; Hamelin, Muriel; Crassous, Marie-pierre; Antoine, Elisabeth; Lehaitre, Michel; Compere, Chantal.
Among marine algae species, Alexandrium minutum produces a phycotoxin called paralytic shellfish poisoning (PSP) that is introduced in the food chain through the ingestion of phytoplankton by shellfishs, and later by human consumers. Thus, in situ monitoring of A. minutum proliferation in coastal seawater is of great economical importance for marine resources exploitation. Here, we propose a rapid test for the detection of A. minutum by surface plasmon resonance spectroscopy. First, whole genomic DNA is extracted from the algae. Second, a 677 bp long portion of the 28S ribosomal DNA is amplified by PCR. Third, the PCR product is detected by surface plasmon resonance spectroscopy onto a DNA functionalized gold substrate.
Tipo: Text
Palavras-chave: PCR; Algal detection; In situ; Spectroscopy; Surface plasmon resonance.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/2009/publication-6830.pdf

Effect of plasmonic mode on plasmon-based lasers
Provedor de dados: 5
Autores: Dridi, Montacer; Colas, Florent; Compere, Chantal.
For the last few years, plasmon-based lasers have been investigated theoretically and experimentally. Several configurations have been reported. They are composed of quantum emitters coupled to a plasmonic structure. In this paper, we investigate the effect of the plasmon mode on the far-field and near-field characteristics of a nanolaser composed by a periodic array of metallic nanoparticles covered by gain materials under optical pumping. Two configurations were investigated. The first structure supported a localized surface plasmon (LSP) mode, while the second a surface lattice plasmon (SLP) mode. This theoretical work relies on semi-quantum calculations based on a four-level gain molecule and a time-domain approach. We demonstrated that lasing in a SLP...
Tipo: Text
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00473/58490/61054.pdf

Finalisation d’un outil préopérationnel FlowCAM / ZooPhytoImage. Action9 - FlowCAM ZooPhytoImage. Livrable n° 3. Rapport final
Provedor de dados: 5
Autores: Wacquet, Guillaume; Lefebvre, Alain; Colas, Florent; Grosjean, Philippe.
Today, the most widely used method for the analysis of samples within the REPHY network, is the microscopy. However, this method has several drawbacks: different skill level depending on the analyst, taxa may be misidentified, analyst subject to fatigue and loss of concentration, non-quantifiable errors. That is why different axis of development have been proposed to adapt the FlowCAM and ZooPhytoImage to the requirements of REPHY network which are the accuracy and repeatability of the measurement. The purpose of these new tools being to provide a tangible gain of time for analysts compared to the observations with optical microscope. The analysis time being a key point of this system, studies were conducted in order to achieve a fast acquisition. To have...
Tipo: Text
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00363/47440/47465.pdf

Surface enhanced infrared absorption (SEIRA) spectroscopy using gold nanoparticles on As2S3 glass
Provedor de dados: 5
Autores: Verger, F.; Pain, T.; Nazabal, V.; Boussard-pledel, C.; Bureau, Bruno; Colas, Florent; Rinnert, Emmanuel; Boukerma, Kada; Compere, Chantal; Guilloux-viry, M.; Deputier, S.; Perrin, A.; Guin, J. P.
The development of infrared sensing platform requires adapted materials. In this work, we used sputtered gold nanoparticles on a chalcogenide glass to demonstrate that these infrared materials can be used as substrate for surface enhanced infrared absorption (SEIRA) spectroscopy. The sputtering parameters were optimized to get the highest possible enhancement. To assess it, a self-assembled monolayer of 4-nitrothiophenol was used. These preliminary results are promising and pave the way for various configurations of waveguide for integrated optical components. (C) 2012 Elsevier B.V. All rights reserved.
Tipo: Text
Palavras-chave: Chalcogenide glass; Sputtering; Gold nanoparticles; 4-Nitrothiophenol; SEIRA; Surface enhanced infrared absorption.
Ano: 2012
URL: http://archimer.ifremer.fr/doc/00116/22748/20613.pdf

Surface Plasmon Resonance sensors for oceanography
Provedor de dados: 5
Autores: Colas, Florent.
Tipo: Text
Palavras-chave: Absolute salinity; Biosensor; Molecular sensor; Refractive index; Surface functionalization; Surface plasmon resonance.
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00630/74235/73866.pdf

Development of microbial and molecular sensors
Provedor de dados: 5
Autores: Boccadoro, Catherine; Karlson, Bengt; Colas, Florent.
The aim of Task 3.4 was to develop and test innovative methods for the molecular detection of phytoplankton, harmful algal blooms and pollutants through their effect on microbial communities. This included the development of novel molecular sensors for the detection, quantification and identification of organisms, microbial markers of pollutant exposure or toxin concentrations in marine coastal waters. Bacterial species and genes which can be used as markers of high nutrient load or hydrocarbon contamination in the marine environment were identified through bacterial community analysis in contaminated environments and literature reviews. Assays using quantitative Polymerase Chain Reaction (qPCR) for the quantification of these organisms were developed and...
Tipo: Text
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00630/74246/73876.pdf

JERICO-NEXT. Progress report after development of microbial and molecular sensors. D3.7
Provedor de dados: 5
Autores: Boccadoro, Catherine; Petersen, Wilhelm; Karlson, Bengt; Colas, Florent.
This progress report includes the work performed during the first two years of the project. During this first phase of the project, much of the focus has been on the development and improvements of the different types of sensors and biosensors before the sensor testing through laboratory and field campains, which will involve sensor tests in a common setting and integration of the results and responses from the different devices and tools.
Tipo: Text
Ano: 2017
URL: http://archimer.ifremer.fr/doc/00423/53432/54320.pdf

A surface plasmon resonance system for the underwater detection of domoic acid
Provedor de dados: 5
Autores: Colas, Florent; Crassous, Marie-pierre; Laurent, Sebastien; Litaker, Richard W.; Rinnert, Emmanuel; Le Gall, Erwan; Lunven, Michel; Delauney, Laurent; Compère, Chantal.
Over the past decade Surface Plasmon Resonance (SPR) techniques have been applied to the measurement of numerous analytes. In this article, an SPR biosensor system deployed from an oceanographic vessel was used to measure dissolved domoic acid (DA), a common and harmful phycotoxin produced by certain microalgae species belonging to the genus Pseudo-nitzschia. During the biosensor deployment, concentrations of Pseudo-nitzschia cells were very low over the study area and measured DA concentrations were below detection. However, the in situ operational detection limit of the system was established using calibrated seawater solutions spiked with DA. The system could detect the toxin at concentrations as low as 0.1 ng mL−1 and presented a linear dynamic range...
Tipo: Text
Ano: 2016
URL: http://archimer.ifremer.fr/doc/00324/43481/42921.pdf

Plasmonic Properties of Aluminum Nanocylinders in the Visible Range
Provedor de dados: 5
Autores: Gillibert, Raymond; Colas, Florent; Yasukuni, Ryohei; Picardi, Gennaro; Lamy De La Chapelle, Marc.
Plasmonic and surface enhanced Raman scattering (SERS) studies have been performed on aluminium nanocylinders arrays of different diameters. We observed sharps localised surface plasmon resonance (LSPR) peaks that can be tuned on the whole visible range and having the same behaviour than gold nanocylinders. The near-field enhancement was measured by SERS on probe molecules as well as on the indium tin oxide (ITO) substrate using two excitation wavelengths: 660 and 785 nm. No SERS signal of the probe molecul
Tipo: Text
Ano: 2017
URL: http://archimer.ifremer.fr/doc/00367/47865/47861.pdf

Organometallic nanoprobe to enhance optical response on the polycyclic aromatic hydrocarbon benzo[a]pyrene immunoassay using SERS technology
Provedor de dados: 5
Autores: Dribek, Mohamed; Rinnert, Emmanuel; Colas, Florent; Crassous, Marie-pierre; Thioune, Nene; David, Catalina; De La Chapelle, Marc; Compere, Chantal.
We demonstrated the use of a new organometallic nanoprobe for competitive surface-enhanced Raman scattering (SERS) immunoassay devoted to the detection of polycyclic aromatic hydrocarbons (PAH) such as benzo[a]pyrene (BaP) in seawater. The nanoprobes are gold nanoparticles (GNPs) labeled by a Raman reporter, the 5,5'-dithiobis(succinimidyl-2-nitrobenzoate) (DSNB) and functionalized with monoclonal antibodies anti-BaP. The antibodies are bound with a high specificity to the analyte while the GNPs enhanced the Raman scattering of the DSNB. This type of immunoassay involved the grafting of BaP onto a sensing surface. Thus, NH2-terminated self-assembled monolayer is formed on the surface of gold substrate using cysteamine. Amines finally reacted with...
Tipo: Text
Palavras-chave: Biosensor; Surface-enhanced Raman scattering; Polycyclic aromatic hydrocarbons; Benzo[a]pyrene antibody; Organometallic nanoprobe.
Ano: 2017
URL: https://archimer.ifremer.fr/doc/00417/52844/53870.pdf

Red-Shift Effects in Surface Enhanced Raman Spectroscopy: Spectral or Intensity Dependence of the Near-Field?
Provedor de dados: 5
Autores: Colas, Florent; Cottat, Maximilien; Gillibert, Raymond; Guillot, Nicolas; Djaker, Nadia; Lidgi-guigui, Nathalie; Toury, Timothee; Barchiesi, Dominique; Toma, Andrea; Di Fabrizio, Enzo; Gucciardi, Pietro G.; De La Chapelle, Marc Lamy.
Optimum amplification in Surface Enhanced Raman Scattering (SERS) from individual nanoantennas is expected when the excitation is slightly blue-shifted with respect to the Localized Surface Plasmon Resonance (LSPR), so that the LSPR peak falls in the middle between the laser and the Stokes Raman emission. Recent experiments have shown when moving the excitation from the visible to the near-infrared that this rule of thumb is no more valid. The excitation has to be red-shifted with respect to the LSPR peak, up to 80nm, to obtain highest SERS. Such discrepancy is usually attributed to a Near-Field (NF) to Far-Field (FF) spectral shift. Here we critically discuss this hypothesis for the case of gold nanocylinders. By combining multi-wavelength excitation SERS...
Tipo: Text
Ano: 2016
URL: https://archimer.ifremer.fr/doc/00341/45234/44668.pdf

Propriétés optiques et thermoplasmoniques de réseaux de nanocylindres: applications à la détection de molécules et de micro-objets
Provedor de dados: 5
Autores: Colas, Florent.
Raman spectroscopy is a technique that is non-invasive, non-destructive, allowing the identiﬁcation of the molecules contained in a solid, liquid or gaseous sample. However it suﬀers from one major drawback : low sensitivity. This limit is now about to be pushed through the fast growth of SERS (Surface Enhanced Raman Scattering). This phenomenon has been already implemented successfully in various applications : biomedical, biology, analytical chemistry, environmental science... However, despite a growing number of scientiﬁc works, some aspects of the SERS sensors still need to be studied. This work focused on the study of the near-ﬁeld and the far-ﬁeld properties of arrays of nanocylinders for the detection of organic compounds. The studied parameters are...
Tipo: Text
Palavras-chave: Plasmon de surface; Diffusion raman exaltee de surface; Champ proche; Champ lointain; Nanocylindres; Thermoplasmonique; Pinces optiques.; Surface plasmon; Near-field; Far-field; Nanocylinder; Thermoplasmonic; Surface enhanced raman scattering; Optical tweezers.
Ano: 2017
URL: https://archimer.ifremer.fr/doc/00620/73191/72385.pdf

Nanoplasmonics tuned “click chemistry”
Provedor de dados: 5
Autores: Tijunelyte, I.; Guenin, E.; Lidgi-guigui, N.; Colas, Florent; Ibrahim, J.; Toury, T.; Lamy De La Chapelle, M.
Nanoplasmonics is a growing field of optical condensed matter science dedicated to optical phenomena at the nanoscale level in metal systems. Extensive research on noble metallic nanoparticles (NPs) has emerged within the last two decades due to their ability to keep the optical energy concentrated in the vicinity of NPs, in particular, the ability to create optical near-field enhancement followed by heat generation. We have exploited these properties in order to induce a localised “click” reaction in the vicinity of gold nanostructures under unfavourable experimental conditions. We demonstrate that this reaction can be controlled by the plasmonic properties of the nanostructures and we propose two physical mechanisms to interpret the observed plasmonic...
Tipo: Text
Ano: 2016
URL: https://archimer.ifremer.fr/doc/00368/47954/48018.pdf

Surface plasmon resonance in chalcogenide glass-based optical system
Provedor de dados: 5
Autores: Le Person, Jenny; Colas, Florent; Compere, Chantal; Lehaitre, Michel; Anne, M; Boussard Pledel, C; Bureau, B; Adam, J; Deputier, S; Guilloux Viry, M.
The surface plasmon resonance phenomenon has been studied in a chalcogenide glass-based optical system. IR transmission properties of these materials combined to their high refractive indices lead to advantageous properties for sensing. In this study, numerical simulations have been carried out to investigate the potentialities of sulfide glass from the GeGaSbS system as a coupling prism material. Then, an angular modulation SPR biosensor has been set up in the Kretschmann-Raether arrangement. Experimental data are consistent with numerical calculation and the detection limit of the sensor is 3 x 10(-5) RIU. These preliminary results are promising. Further investigations have to be carried out to confirm the great potentialities of those materials for...
Tipo: Text
Palavras-chave: Infrared; Biosensor; Surface plasmon resonance; Chalcogenide glass.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/publication-3955.pdf

RF sputtered amorphous chalcogenide thin films for surface enhanced infrared absorption spectroscopy
Provedor de dados: 5
Autores: Verger, F.; Nazabal, V.; Colas, Florent; Nemec, P.; Cardinaud, C.; Baudet, E.; Chahal, R.; Rinnert, Emmanuel; Boukerma, Kada; Peron, Isabelle; Deputier, S.; Guilloux-viry, M.; Guin, J. P.; Lhermite, H.; Moreac, A.; Compere, Chantal; Bureau, Bruno.
The primary objective of this study is the development of transparent thin film materials in the IR enabling strong infrared absorption of organic compounds in the vicinity of metal nanoparticles by the surface plasmon effect. For developing these optical micro-sensors, hetero-structures combining gold nanoparticles and chalcogenide planar waveguides are fabricated and adequately characterized. Single As2S3 and Ge25Sb10Se65 amorphous chalcogenide thin films are prepared by radio-frequency magnetron sputtering. For the fabrication of gold nanoparticles on a chalcogenide planar waveguide, direct current sputtering is employed. Fabricated single layers or hetero-structures are characterized using various techniques to investigate the influence of deposition...
Tipo: Text
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00170/28094/26679.pdf

Design, Characterization, and Test of a Versatile Single-Mode Power-Over-Fiber and Communication System for Seafloor Observatories
Provedor de dados: 5
Autores: Diouf, Cherif; Quintard, Veronique; Ghisa, Laura; Guegan, Mikael; Perennou, Andre; Gautier, Laurent; Tardivel, Morgan; Barbot, Stephane; Dutreuil, Vincent; Colas, Florent.
A power-over-fiber (PoF) and communication system for extending a cabled seafloor observatory is demonstrated in this contribution. The system allows the cabled seafloor observatory to be linked, through a single optical fiber, to a sensor node located 8 km away. The PoF system is based on an optical architecture in which power and data propagate simultaneously on the same single-mode fiber. The Raman scattering effect is exploited to amplify the optical data signals and leads to the minimization of the sensor node power consumption. Versatile low power electronic interfaces have been developed to ensure compatibility with a wide range of marine sensors. A low-consumption field-programmable gate array and an energy-efficient microcontroller are used to...
Tipo: Text
Palavras-chave: Low power embedded systems; Power-over-fiber (PoF); Raman amplification; Remote sensing; Seafloor observatories.
Ano: 2020
URL: https://archimer.ifremer.fr/doc/00465/57684/59933.pdf

Organometallic nanoprobe to enhance optical response on Polycyclic Aromatic Hydrocarbons (PAHs) immunoassay using SERS technology
Provedor de dados: 5
Autores: Dribek, Mohamed; Rinnert, Emmanuel; Colas, Florent; Crassous, Marie-pierre; Compere, Chantal.
We demonstrate the possible use of organometallic nanoprobe on competitive surface-enhanced Raman scattering immunoassay for policyclic aromatic hydrocarbon detection in sea water. This type of SERS immunoassay is based on to capture Raman reporter-labeled immuno-Au onto the immobilized layer of antigens (benzo[a]pyrene) on the gold substrate after incubation of these nanoprobes with PAHs including in the sample. To realize nanoprobes, first we have prepared gold nanoparticles (GNPs) according to Frens’ method. Then, we have labeled GNPs by the Raman reporter of 5,5’-Dithiobis(succinimidyl-2-nitrobenzoate) (DSNB). Thereafter, Benzo[a]pyrene antibody was attached to the DSNB labeled GNPs. Before use in SERS detection, their activity was controlled by...
Tipo: Text
Ano: 2012
URL: http://archimer.ifremer.fr/doc/00088/19912/17576.pdf

Toward Chalcogenide Platform Infrared Sensor Dedicated to the In Situ Detection of Aromatic Hydrocarbons in Natural Waters via an Attenuated Total Reflection Spectroscopy Study
Provedor de dados: 5
Autores: Baillieul, Marion; Baudet, Emeline; Michel, Karine; Moreau, Jonathan; Němec, Petr; Boukerma, Kada; Colas, Florent; Charrier, Joël; Bureau, Bruno; Rinnert, Emmanuel; Nazabal, Virginie.
he objective of this study is to demonstrate the successful functionalization of the surface of a chalcogenide infrared waveguide with the ultimate goal of developing an infrared micro-sensor device. First, a polyisobutylene coating was selected by testing its physico-chemical compatibility with a Ge-Sb-Se selenide surface. To simulate the chalcogenide platform infrared sensor, the detection of benzene, toluene, and ortho-, meta- and para-xylenes was efficaciously performed using a polyisobutylene layer spin-coated on 1 and 2.5 µm co-sputtered selenide films of Ge28Sb12Se60 composition deposited on a zinc selenide prism used for attenuated total reflection spectroscopy. The thickness of the polymer coating was optimized by attenuated total reflection...
Tipo: Text
Palavras-chave: Optical infrared sensor; Chalcogenide glasses; Mid-infrared; Mono-aromatic hydrocarbons; Natural waters; BTEXs; PAHs.
Ano: 2021
URL: https://archimer.ifremer.fr/doc/00687/79947/82867.pdf

The ZooCAM, a new in-flow imaging system for fast onboard counting, sizing and classification of fish eggs and metazooplankton
Provedor de dados: 5
Autores: Colas, Florent; Tardivel, Morgan; Perchoc, Jonathan; Lunven, Michel; Forest, Bertrand; Guyader, Gerard; Danielou, Marie-madeleine; Le Mestre, Sophie; Bourriau, Paul; Antajan, Elvire; Sourisseau, Marc; Huret, Martin; Petitgas, Pierre; Romagnan, Jean-baptiste.
In this paper we present the ZooCAM, a system designed to digitize and analyse on board large volume samples of preserved and living metazooplankton (i.e. multicellular zooplankton) and fish eggs &gt; 300 µm ESD. The ZooCAM has been specifically designed to overcome the difficulties to analyse zooplankton and fish eggs in the framework of the PELGAS survey, and provide high frequency data. The ZooCAM fish eggs counts were comparable to those done with a dissecting microscope. The ZooCAM enabled the accurate prediction and fast on board validation of staged anchovy and sardine eggs in almost real time after collection. A comparison with the ZooScan, on a more complex zooplanktonic community, provided encouraging results on the agreement between the 2...
Tipo: Text
Palavras-chave: ZooCAM; In-flow imaging; Fish eggs; Zooplankton; Ecosystemic survey.
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00407/51833/52432.pdf

New Approach For The Detection Of Toxic Algae Species
Provedor de dados: 5
Autores: Colas, Florent; Crassous, Marie-pierre; Laurent, Sebastien; Dreanno, Catherine; Compere, Chantal.
Up to now, toxic species are monitored by expensive and time-consuming laboratory analysis of seawater samples: after a 24h decantation, the samples are observed with an optical microscope and the cell concentration per species is determined. In this context, systems able to detect HAB species directly underwater would be of great help. An assay based on an SPR biosensor was then developed for detecting Alexandrium minutum.
Tipo: Text
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00089/19995/17658.pdf

First Steps of in Situ Surface-Enhanced Raman Scattering During Shipboard Experiments
Provedor de dados: 5
Autores: Peron, Olivier; Rinnert, Emmanuel; Colas, Florent; Lehaitre, Michel; Compere, Chantal.
It is shown that the surface-enhanced Raman scattering (SERS) technique can be applied to detect organic molecules during in situ experiments. To this purpose, we used trans-1,2-bis(4-pyridyl)ethylene (BPE) as a target molecule. Adsorbed on the SERS chemosensor surface and excited under laser, the vibration modes of the molecules can be identified. SERS chemosensors are based on quartz substrates functionalized by silanization and partially coated with gold nanoparticles. SERS measurements during shipboard experiments were made with a home-made in situ Raman spectrometer connected to a marinized micro-fluidic system. The device was designed to host chemosensors in order to ensure measurements with a flow cell. A theoretical limit of detection was estimated...
Tipo: Text
Palavras-chave: Surface-enhanced Raman scattering; SERS; Chemosensors; Flow cells; Shipboard experiments; In situ measurements; Freundlich isotherm.
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00016/12691/9718.pdf

Towards in situ detection of PAH trace in sea-water using SERS-active sensors
Provedor de dados: 5
Autores: Peron, Olivier; Rinnert, Emmanuel; Lehaitre, Michel; Colas, Florent; Compere, Chantal.
This paper reports the development of a sensor based on surface-enhanced Raman scattering (SERS) for analyses in sea-water. Polycyclic aromatic hydrocarbons (PAHs) are targeted by these sensors and their detection in situ summons up chemical synthesis and optical development. Firstly, a relevant synthesis of SERS active substrates based on gold nanostructures is presented. Different kinds of substrates have been synthesized under variable experimental conditions to modify some parameters such as i) gold shape, size and distribution and such as ii) chemical functionalization: (i) gold nanoparticles were prepared either by chemical reduction of HAuCl4 or by physical deposition. (ii) Substrates were functionalized by hydrophobic films to allow nonpolar...
Tipo: Text
Palavras-chave: Functionalization; Silanization; Gold nanostructures; SERS; PAH detection; Sea-water; Sensor; In situ.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/00012/12363/9152.pdf