Consulta: creatorFacets:"Compere, Chantal"
Registros recuperados: 63
Data/hora: 06/06/2024 04:31:25

Toward in situ detection of algae species
Provedor de dados: 5
Autores: Colas, Florent; Laurent, Sebastien; Lehaitre, Michel; Hamelin, Muriel; Crassous, Marie-pierre; Antoine, Elisabeth; Dreanno, Catherine; Deuff, Albert; Compere, Chantal.
For the last few years there is a more and more pressing need to develop system for detecting HAB at their early stage. Researchers have then been working on new in situ sensor. In this paper a submarine transducer based on surface plasmon resonance is reported. It demonstrated a refractive index resolution of 3.10(-6), which is promising for later phytoplankton detection.
Tipo: Text
Ano: 2009
URL: http://archimer.ifremer.fr/doc/00107/21802/20328.pdf

Compte Rendu d’Activités 2012 de l’Unité de Recherche « Recherches et Développements Technologiques »
Provedor de dados: 5
Autores: Compere, Chantal; Unité RDT.
Compte Rendu d’Activités 2012 de l’Unité de Recherche « Recherches et Développements Technologiques »
Tipo: Text
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00171/28240/26493.pdf

Chalcogenide Glass Optical Waveguides for Infrared Biosensing
Provedor de dados: 5
Autores: Anne, Marie-laure; Keirsse, Julie; Nazabal, Virginie; Hyodo, Koji; Inoue, Satoru; Boussard-pledel, Catherine; Lhermite, Herve; Charrier, Joel; Yanakata, Kiyoyuki; Loreal, Olivier; Le Person, Jenny; Colas, Florent; Compere, Chantal; Bureau, Bruno.
Due to the remarkable properties of chalcogenide (Chg) glasses, Chg optical waveguides should play a significant role in the development of optical biosensors. This paper describes the fabrication and properties of chalcogenide fibres and planar waveguides. Using optical fibre transparent in the mid-infrared spectral range we have developed a biosensor that can collect information on whole metabolism alterations, rapidly and in situ. Thanks to this sensor it is possible to collect infrared spectra by remote spectroscopy, by simple contact with the sample. In this way, we tried to determine spectral modifications due, on the one hand, to cerebral metabolism alterations caused by a transient focal ischemia in the rat brain and, in the other hand, starvation...
Tipo: Text
Palavras-chave: Planar waveguide; Fibre; Optical sensor; Chalcogenide.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/2009/publication-6851.pdf

New SERS Substrates For Polycyclic Aromatic Hydrocarbon (PAH) Detection: Towards Quantitative SERS Sensors For Environmental Analysis
Provedor de dados: 5
Autores: Peron, Olivier; Rinnert, Emmanuel; Compere, Chantal; Toury, T.; De La Chapelle, M. Lamy.
In the investigation of chemical pollutions, such as PAHs (Polycyclic Aromatic Hydrocarbons) at low concentration in aqueous medium, surface-enhanced Raman scattering (SERS) stands for an alternative to the inherent low cross-section of normal Raman scattering. Indeed, SERS is a very sensitive spectroscopic technique due to the excitation of the surface plasmon modes of the nanostructured metallic film.
Tipo: Text
Palavras-chave: Quantitative SERS sensors; Polystyrene beads; PAHs; Nanoparticles.
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00012/12328/9113.pdf

Testing methods to assess both the efficacy and ecotoxicity of antifouling coatings
Provedor de dados: 5
Autores: Quiniou, Francoise; Compere, Chantal; Caisey, Xavier; Davy, Romain; Matarere, Marcel; Mazeas, Florence; Peleau, Michel.
Since 1998, the European Biocidal Products Directive (BPD; 98/8/EC), applied to 23 product types (PT) for specific uses, including PT 21 the « antifouling products », regulates biocidal products before they are placed on the market.  On the one hand, the efficacy of active substances and their formulations must be demonstrated for their specific employment ; on the other hand their non impact on workers neither users and environment must be proved and specifically against non target species.  Standard protocols for the assessment of efficacy or ecotoxicity of antifouling substances are proposed within the framework of the Ecopaint and Paintclean projects which are founded by the french industry Ministery and labellised by the “Pôle Mer” (Paca and...
Tipo: Text
Ano: 2009
URL: http://archimer.ifremer.fr/doc/00040/15133/12471.pdf

Bioactive Compounds Produced by Pseudoalteromonas Affect Marine Biofilm Formation
Provedor de dados: 5
Autores: Klein, Geraldine; Soum-soutera, Emmanuelle; Bazire, Alexis; Dreanno, Catherine; Compere, Chantal; Dufour, Alain.
Bacterial biofilms, frequently in association with algae, protozoa and fungi, are found on all submerged structures in the marine environment. Biofilms are responsible for a range of surface‐associated and diffusible signals, which may moderate the settling behaviour of cells, spores and larvae. Thus, marine microorganisms are a new source of bioactive compounds, which enhance or inhibit the settlement of organisms.
Tipo: Text
Ano: 2009
URL: http://archimer.ifremer.fr/doc/00089/20005/17667.pdf

Biofouling protection for marine environmental sensors
Provedor de dados: 5
Autores: Delauney, Laurent; Compere, Chantal; Lehaitre, Michel.
These days, many marine autonomous environment monitoring networks are set up in the world. These systems take advantage of existing superstructures such as offshore platforms, lightships, piers, breakwaters or are placed on specially designed buoys or underwater oceanographic structures. These systems commonly use various sensors to measure parameters such as dissolved oxygen, turbidity, conductivity, pH or fluorescence. Emphasis has to be put on the long term quality of measurements, yet sensors may face very short-term biofouling effects. Biofouling can disrupt the quality of the measurements, sometimes in less than a week. Many techniques to prevent biofouling on instrumentation are listed and studied by researchers and manufacturers. Very few of them...
Tipo: Text
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00008/11920/8626.pdf

Immobilization of Protein A on SAMS for the elaboration of immunosensors
Provedor de dados: 5
Autores: Briand, Elisabeth; Salmain, Michèle; Compere, Chantal; Pradier, Claire-marie.
Binary mixtures of 11-mercaptoundecanoic acid (MUA) and other thiols of various lengths and terminal functions were chemisorbed on gold-coated surfaces via S-Au bonds to form mixed self-assembled monolayers (SAMs). Several values of the inole fraction of MUA in the thiol mixtures were tested and the structure and composition of the resulted thin films were characterized by X-ray photoelectron spectroscopy (XPS) and polarization modulation infrared reflection-absorption spectroscopy (PM-IRRAS). The results made it clear that co-adsorption of MUA with thiols of similar chain length led to well-ordered monolayers whereas the co-adsorption of MUA with shorter thiols yielded less crystalline-like thin films, but with more reactive carboxylic acid terminal...
Tipo: Text
Palavras-chave: PM IRRAS; Antibody; Protein A; Gold; Self assembled monolayers.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/publication-2222.pdf

Antibiofilm Activity of the Marine Bacterium Pseudoalteromonas sp Strain 3J6
Provedor de dados: 5
Autores: Dheilly, Alexandra; Soum-soutera, Emmanuelle; Klein, Geraldine; Bazire, Alexis; Compere, Chantal; Haras, Dominique; Dufour, Aurelie.
Biofilm formation results in medical threats or economic losses and is therefore a major concern in a variety of domains. In two-species biofilms of marine bacteria grown under dynamic conditions, Pseudoalteromonas sp. strain 3J6 formed mixed biofilms with Bacillus sp. strain 4J6 but was largely predominant over Paracoccus sp. strain 4M6 and Vibrio sp. strain D01. The supernatant of Pseudoalteromonas sp. 3J6 liquid culture (SN3J6) was devoid of antibacterial activity against free-living Paracoccus sp. 4M6 and Vibrio sp. D01 cells, but it impaired their ability to grow as single-species biofilms and led to higher percentages of nonviable cells in 48-h biofilms. Antibiofilm molecules of SN3J6 were able to coat the glass surfaces used to grow biofilms and...
Tipo: Text
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00004/11530/11317.pdf

Toward Detection of Harmful Algae Blooms by in situ Surface Plasmon Resonance Spectroscopy
Provedor de dados: 5
Autores: Laurent, Sebastien; Colas, Florent; Hamelin, Muriel; Crassous, Marie-pierre; Antoine, Elisabeth; Lehaitre, Michel; Compere, Chantal.
Among marine algae species, Alexandrium minutum produces a phycotoxin called paralytic shellfish poisoning (PSP) that is introduced in the food chain through the ingestion of phytoplankton by shellfishs, and later by human consumers. Thus, in situ monitoring of A. minutum proliferation in coastal seawater is of great economical importance for marine resources exploitation. Here, we propose a rapid test for the detection of A. minutum by surface plasmon resonance spectroscopy. First, whole genomic DNA is extracted from the algae. Second, a 677 bp long portion of the 28S ribosomal DNA is amplified by PCR. Third, the PCR product is detected by surface plasmon resonance spectroscopy onto a DNA functionalized gold substrate.
Tipo: Text
Palavras-chave: PCR; Algal detection; In situ; Spectroscopy; Surface plasmon resonance.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/2009/publication-6830.pdf

Building of an immunosensor: How can the composition and structure of the thiol attachment layer affect the immunosensor efficiency?
Provedor de dados: 5
Autores: Briand, Elisabeth; Salmain, Michèle; Henry, Jean-marie; Perrot, Hubert; Compere, Chantal; Pradier, Claire-marie.
Immunosensors, based on the immobilization of a model rabbit antibody on mixed self-assembled monolayers and Protein A as a linking agent on gold transducers, were elaborated and characterized at each step by modulated polarization-infrared spectroscopy (PM-IRRAS) and occasionally by atomic force microscopy (AFM) and quartz crystal microbalance (QCM). By testing two different mixed SAMs comprising 11-mercaptoundecanoic acid (MUA), together with either decanethiol (C9CH3) or mercaptohexanol (C6OH), the role of the chemical composition and structure of the antibody attachment layer upon the sensor performance was demonstrated. (c) 2006 Elsevier B.V. All rights reserved.
Tipo: Text
Palavras-chave: QCM; IRRAS; Thiol attachment; Immunosensor; Mixed SAM' s.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/publication-1988.pdf

Extrapolation of the functions of the International Temperature Scale of 1990
Provedor de dados: 5
Autores: Salvetat, Florence; Le Bihan, Caroline; Compere, Chantal.
Tipo: Text
Ano: 2011
URL: http://archimer.ifremer.fr/doc/00063/17447/14952.pdf

Compte Rendu d’Activités 2015 de l’Unité de Recherche « Recherches et Développements Technologiques »
Provedor de dados: 5
Autores: Compere, Chantal; Ifremer, REM/RDT.
Tipo: Text
Ano: 2016
URL: https://archimer.ifremer.fr/doc/00346/45748/45389.pdf

De nouvelles voies d’inhibition des biofilms. Des micro-organismes producteurs de nouvelles molécules actives
Provedor de dados: 5
Autores: Klein, Geraldine; Dufour, Alain; Compere, Chantal.
Lors d’une immersion en eau de mer, tous les matériaux et structures sont rapidement colonisés par des salissures d’origine biologique : des biofilms. La transformation des propriétés de surface dumatériau et la présence demicro- oumacro-organismes (bactéries, algues, balanes, larves) engendrent des risques accrus de corrosion localisée, la biodétérioration desmatériaux immergés, le blocage des fonctions mécaniques…Afin de contrôler le dépôt et le développement de ces biofilms, la méthode privilégiée est l’application de peintures anti-salissures. Cependant ces revêtements sont généralement toxiques pour l’environnement. C’est pourquoi, les travaux menés actuellement visent à isoler de nouvelles molécules, produites par les bactéries marines, dans le but...
Tipo: Text
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00351/46213/45910.pdf

Effect of plasmonic mode on plasmon-based lasers
Provedor de dados: 5
Autores: Dridi, Montacer; Colas, Florent; Compere, Chantal.
For the last few years, plasmon-based lasers have been investigated theoretically and experimentally. Several configurations have been reported. They are composed of quantum emitters coupled to a plasmonic structure. In this paper, we investigate the effect of the plasmon mode on the far-field and near-field characteristics of a nanolaser composed by a periodic array of metallic nanoparticles covered by gain materials under optical pumping. Two configurations were investigated. The first structure supported a localized surface plasmon (LSP) mode, while the second a surface lattice plasmon (SLP) mode. This theoretical work relies on semi-quantum calculations based on a four-level gain molecule and a time-domain approach. We demonstrated that lasing in a SLP...
Tipo: Text
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00473/58490/61054.pdf

Detection of polycyclic aromatic hydrocarbon (PAH) compounds in artificial sea-water using surface-enhanced Raman scattering (SERS)
Provedor de dados: 5
Autores: Peron, Olivier; Rinnert, Emmanuel; Lehaitre, Michel; Crassous, Philippe; Compere, Chantal.
This paper reports an accurate synthesis of surface-enhanced Raman scattering (SERS) active substrates, based on gold colloidal monolayer, suitable for in situ environmental analysis. Quartz substrates were functionalized by silanization with (3-mercaptopropyl)trimethoxysilane (MPMS) or (3-aminopropyl)trimethoxysilane (APTMS) and they subsequently reacted with colloidal suspension of gold metal nanoparticles: respectively, the functional groups SH and NH2 bound gold nanoparticles. Gold nanoparticles were prepared by the chemical reduction of HAuCl4 using sodium tricitrate and immobilized onto silanized quartz substrates. Active substrate surface morphology was characterized with scanning electron microscopy (SEM) measurements and gold nanoparticles...
Tipo: Text
Palavras-chave: Sensor; Sea water; PAH detection; SERS; Silanization; Gold nanoparticles.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/2009/publication-6616.pdf

Development of a Mass Sensitive Quartz Crystal Microbalance (QCM)-Based DNA Biosensor Using a 50 MHz Electronic Oscillator Circuit
Provedor de dados: 5
Autores: Garcia-martinez, Gonzalo; Alonso Bustabad, Enrique; Perrot, Hubert; Gabrielli, Claude; Bucur, Bogdan; Lazerges, Mathieu; Rose, Daniel; Rodriguez-pardo, Loreto; Farina, Jose; Compere, Chantal; Arnau Vives, Antonio.
This work deals with the design of a high sensitivity DNA sequence detector using a 50 MHz quartz crystal microbalance (QCM) electronic oscillator circuit. The oscillator circuitry is based on Miller topology, which is able to work in damping media. Calibration and experimental study of frequency noise are carried out, finding that the designed sensor has a resolution of 7.1 ng/cm(2) in dynamic conditions (with circulation of liquid). Then the oscillator is proved as DNA biosensor. Results show that the system is able to detect the presence of complementary target DNAs in a solution with high selectivity and sensitivity. DNA target concentrations higher of 50 ng/mL can be detected.
Tipo: Text
Palavras-chave: DNA sequence detection; Quartz crystal microbalance; Electronic oscillator; Sensitivity; Frequency noise; Resolution.
Ano: 2011
URL: http://archimer.ifremer.fr/doc/00044/15487/12886.pdf

Biofilms en mileu marin
Provedor de dados: 5
Autores: Compere, Chantal.
Introduction: Almost all submerged surfaces in the marine environment are subject to developing an organic film, with the exception of some living organisms, such as sponges, which do not undergo any great colonization, for no currently-known scientific reason. The presence of this biofilm is at the source of many problems in the oceanographic field and for marine business. This article introduces some drawbacks relating to the existence of this fouling and elaborates upon the main studies conducted at IFREMER concerning the most-used methods for combating it. However, the anti-fouling agents that are generally used for protecting facilities are toxic and can have disastrous consequences on the marine environment's fauna and flora. Moreover, the use of...
Tipo: Text
Palavras-chave: Biofilm; Seawater.
Ano: 1999
URL: http://archimer.ifremer.fr/doc/1999/publication-1698.pdf

DNA hybridization mechanism in an interfacial environment: What hides beneath first order k (s(-1)) kinetic constant?
Provedor de dados: 5
Autores: Lazerges, M.; Perrot, H.; Rabehagasoa, N.; Compere, Chantal; Dreanno, Catherine; Mucio Pedroso, M.; Faria, R. C.; Bueno, P. R.
The scientific question addressed in this work is: what hides beneath first order kinetic constant k (s(-1)) measured for hybridization of a DNA target on a biosensor surface. Kinetics hybridization curves were established with a 27 MHz quartz microbalance (9 MHz, third harmonic) biosensor, constituted of a 20-base probe monolayer deposited on a gold covered quartz surface. Kinetics analysis, by a known two-step adsorption-hybridization mechanism, is well appropriate to fit properly hybridization kinetics curves, for complementary 20-base to 40-base targets over two concentration decades. It was found that the K-1 (M-1) adsorption constant, relevant to the first step, concerns an equilibrium between non hybridized targets and hybridized pre-complex and...
Tipo: Text
Palavras-chave: Biosensors; DNA; Hybridization kinetics; Quartz crystal microbalance.
Ano: 2012
URL: http://archimer.ifremer.fr/doc/00101/21195/19415.pdf

Surface enhanced infrared absorption (SEIRA) spectroscopy using gold nanoparticles on As2S3 glass
Provedor de dados: 5
Autores: Verger, F.; Pain, T.; Nazabal, V.; Boussard-pledel, C.; Bureau, Bruno; Colas, Florent; Rinnert, Emmanuel; Boukerma, Kada; Compere, Chantal; Guilloux-viry, M.; Deputier, S.; Perrin, A.; Guin, J. P.
The development of infrared sensing platform requires adapted materials. In this work, we used sputtered gold nanoparticles on a chalcogenide glass to demonstrate that these infrared materials can be used as substrate for surface enhanced infrared absorption (SEIRA) spectroscopy. The sputtering parameters were optimized to get the highest possible enhancement. To assess it, a self-assembled monolayer of 4-nitrothiophenol was used. These preliminary results are promising and pave the way for various configurations of waveguide for integrated optical components. (C) 2012 Elsevier B.V. All rights reserved.
Tipo: Text
Palavras-chave: Chalcogenide glass; Sputtering; Gold nanoparticles; 4-Nitrothiophenol; SEIRA; Surface enhanced infrared absorption.
Ano: 2012
URL: http://archimer.ifremer.fr/doc/00116/22748/20613.pdf

Adhésion et substances polymériques extracellulaires d’une nouvelle espèce bactérienne marine
Provedor de dados: 5
Autores: Leroy, Celine; Compere, Chantal; Delbarre Ladrat, Christine; Raguenes, Gerard; Ghillebaert, François; Combes, Didier.
Une nouvelle bactérie marine du genre Pseudoalteromonas sp., D41, formant rapidement des biofilms sur tout type de matériau (verre, acier inoxydable, Téflon) a été isolée en Baie de Brest. L’objectif de ce présent travail est de caractériser et décrire cette bactérie capable de produire des exopolymères constitués en majorité de protéines. Le rôle des protéines sécrétées, dans les mécanismes d’adhésion, est discuté.
Tipo: Text
Ano: 2005
URL: http://archimer.ifremer.fr/doc/00088/19950/17617.pdf

Monoclonal antibody against the surface of Alexandrium minutum used in a whole-cell ELISA
Provedor de dados: 5
Autores: Gas, F; Pinto, L; Baus, B; Gaufres, L; Crassous, Marie-pierre; Compere, Chantal; Quemeneur, E.
Harmful algal blooms represent a major threat to marine production, and particularly to shellfish farming. Alexandrium minutum, which causes paralytic shellfish poisoning, is occurring with increasing frequency along European coasts. Current regulatory methods to analyze environmental samples are tedious and time consuming because they require taxonomists and involve animal experiments. New rapid detection methods, such as immunoassays, are needed to ensure a fast alert system and for field studies of algal ecodynamics. Rat monoclonal antibodies were raised and selected for their ability to specifically recognize a surface antigen for the A. minutum strain AM89BM from the Bay of Morlaix, France. A whole-cell ELISA was designed, leading to the selection of...
Tipo: Text
Palavras-chave: Monoclonal antibody; Harmful algal bloom; Enzyme linked immunosorbent assay; Dinoflagellate.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/2008/publication-4647.pdf

Detection and molecular identification of Pseudo-nitzschia species in natural samples from the French coasts
Provedor de dados: 5
Autores: Perez Blanco, Eva; Antoine, Elisabeth; Crassous, Marie-pierre; Compere, Chantal.
Domoic acid (DA) was first detected in France in mussel flesh in 1998, outbreak that was linked to the presence of Pseudo-nitzschia pseudodelicatissima. Ever since, the phytoplankton monitoring program (REPHY, Réseau de surveillance du Phytoplancton et des Phycotoxines) also monitors DA content in shellfish flesh. Amnesic shellfish poisoning (ASP) has been recurrently detected, and was over the sanitary threshold in the years 2000 and 2004 in Brittany and in 2002 in the Mediterranean coast (Amzil, 2001; Amzil pers. comm.). Toxicity events are usually dominated by several Pseudo-nitzschia species, although different strains might be or not be toxic (Bates et al., 1998). This is why an inventory of the species present in the French coasts and their molecular...
Tipo: Text
Ano: 2008
URL: http://archimer.ifremer.fr/doc/00089/19988/17652.pdf

Effects of commercial enzymes on the adhesion of a marine biofilm-forming bacterium
Provedor de dados: 5
Autores: Leroy, C.; Delbarre Ladrat, Christine; Ghillebaert, F.; Compere, Chantal; Combes, D.
The antifouling potential of commercial hydrolases, four proteases, seven glycosidases and one lipase was evaluated on the adhesion of marine Pseudoalteromonas sp. D41. The experimental method, adapted to screen antifouling agents, was based on bacterial adhesion in natural sterile sea water in a microtiter plate and on total biomass quantification by the fluorescent dye DAPI (4[prime]6-diamidino-2-phenylindole). Savinase (subtilisin) was the most effective hydrolase in both the prevention of bacterial adhesion and the removal of adhered bacteria. However, some enzymatic preparations tested such as Amano protease were not only ineffective but also increased the number of adhered bacterial cells. Enumeration using epifluorescence microscopy of CTC...
Tipo: Text
Palavras-chave: Marine biofilm; Antifouling; Pseudoalteromonas; Enzymes; Adhesion.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/publication-6140.pdf

Oligonucleotide quartz crystal microbalance sensor for the microalgae Alexandrium minutum (Dinophyceae)
Provedor de dados: 5
Autores: Lazerges, M; Perrot, H; Antoine, Elisabeth; Defontaine, Audrey; Compere, Chantal.
We report the immobilization on a gold surface of a 20-base DNA probe labeled with disulfide group and on the selective hybridization with the complementary 20-base DNA strand. The oligonucleotide probe is the complementary strand of a partial sequence of the gene encoding for a large ribosomal RNA sub-unit which is a coding sequence of Alexandrium minutum DNA, a microalgae that produces neurotoxins responsible for paralytic shellfish poisoning on European and Asian coasts. The kinetics of DNA probe immobilization and hybridization were monitored in situ by using a 27 MHz quartz crystal microbalance under controlled hydrodynamic conditions. The frequency of the setup is stable to within a few hertz, corresponding to the nanogram, scale, for 3 h and makes...
Tipo: Text
Palavras-chave: DNA; Alexandrium minutum; Microbalance.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/publication-1354.pdf

Ficopomatus enigmaticus, Ecologie, répartition en Bretagne et en France, nuisances et moyens de lutte sur le site atelier du port de Vannes
Provedor de dados: 5
Autores: Camus, Patrick; Compere, Chantal; Blanchet, Aline; Dimeet, Joel; Hamon, Dominique; Lacotte, Nicolas; Peleau, Michel; Lassalle, Elisabeth.
This study [...] was carried out from June 1998 to January 2000 and enabled the assessment of the scientific knowledge on the biology, ecology and distribution of the Annelida Polychaeta sedentaria: Ficopomatus enigmaticus. [...] In order to better determine the characteristics of this species' environment and development cycle, a study was conducted in the worksite of the marina of Vannes. This study, mostly biological, was completed by a technological study on the fighting techniques (antifouling paints) used against the damage made by this worm on the boat hulls. In addition, this document presents some recommendations to follow in order to limit, or stop, the development of this "pollution".
Tipo: Text
Palavras-chave: Marina harbours; Disturbance; Colonization; Ficopomatus enigmaticus; Port de plaisance; Pertubation; Colonisation; Ficopomatus enigmaticus.
Ano: 2000
URL: http://archimer.ifremer.fr/doc/2000/rapport-6131.pdf

Les Biotechnologies Marines dans le Grand Ouest
Provedor de dados: 5
Autores: Boyen, Catherine; Jaouen, Pascal; Blanchard, Gilbert; Compere, Chantal; Dufour, Alain; Durand, Patrick; Guerard, Fabienne; Hallouin, Florence; Jebbar, Mohamed; Le Blay, Gwenaelle; Le Deit, Hervé; Le Seyec, Jocelyne; Monks, Brian; Portal-sellin, Rachel; Probert, Ian; Pruvost, Jeremy.
Marine (= blue) biotechnology, i.e. the utilization of marine bio-resources as a target or source of biotechnological applications, is a field with massive potential for innovation and economic growth. In a context of rapid climate change and increasing pressure on natural resources, renewed interest in marine biotechnology has been promoted by application of recent methodological and technological advances, notably in bioprocessing and in the various –omics domains, to the study of marine biodiversity. Marine biological resources potentially represent a sustainable raw material for exploitation in diverse fields, including nutrition, health, agriculture, aquaculture, energy, environment, and cosmetics. Marine biotechnology is now recognized as a...
Tipo: Text
Ano: 2015
URL: http://archimer.ifremer.fr/doc/00273/38460/36860.pdf

Studies of a full-scale mechanical prototype line for the ANTARES neutrino telescope and tests of a prototype instrument for deep-sea acoustic measurements
Provedor de dados: 5
Autores: Ageron, M; Aguilar, J; Albert, A; Ameli, F; Anghinolfi, M; Anton, G; Anvar, S; Ardellier Desages, F; Aslanides, E; Aubert, J; Auer, R; Barbarito, E; Basa, S; Battaglieri, M; Bazzotti, M; Becherini, Y; Bethoux, N; Beltramelli, J; Bertin, V; Bigi, A; Billault, M; Blaes, R; De Botton, N; Bouwhuis, M; Bruijn, R; Brunner, J; Burgio, G; Busto, J; Cafagna, F; Caillat, L; Calzas, A; Capone, A; Caponetto, L; Carmona, E; Carr, J; Castel, D; Castorina, E; Cavasinni, V; Ceechini, S; Ceres, A; Charvis, P; Chauchot, Pierre; Chiarusi, T; Circella, M; Coail, Jean-yves; Colnard, C; Compere, Chantal; Coniglione, R; Cottini, N; Coyle, P; Cuneo, S; Cussatlegras, A; Damy, Gilbert; Van Dantzig, R; Debonis, G; De Marzo, C; De Vita, R; Dekeyser, I; Delagnes, E; Denans, D; Deschamps, Anne; Dessa, J; Destelle, J; Dinkespieler, B; Distefano, C; Donzaud, C; Drogou, Jean-francois; Druillole, F; Durand, D; Ernenwein, J; Escoffier, S; Falchini, E; Favard, S; Fehr, F; Feinstein, F; Florello, C; Flaminio, V; Fratini, K; Fuda, J; Galeotti, S; Gallone, J; Giacomelli, G; Girard, N; Gojak, C; Goret, P; Graf, K; Guilloux, F; Hallewell, G; Harakeh, M; Hartmann, B; Heijboer, A; Heine, E; Hello, Y; Hernandez Rey, J; Hossl, J; Hoffman, C; Hogenbirk, J; Hubbard, J; Jaquet, M; Jaspers, M; De Jong, M; Jouvenot, F; Kalantar Nayestanaki, N; Kappes, A; Karg, T; Katz, U; Keller, P; Kneib, J; Kok, E; Kok, H; Kooijman, P; Kopper, C; Kouchner, A; Kretschmer, W; Kruijer, A; Kuch, S; Lagier, P; Lahmann, R; Lamanna, G; Lamare, P; Lambard, G; Languillat, J; Laschinsky, H; Lavalle, J; Le Guen, Yvon; Le Provost, H; Van Suu, A; Lefevre, D; Legou, T; Lelaizant, G; Lim, G; Lo Presti, D; Loaec, Gerard; Loehner, H; Loucatos, S; Louis, F; Lucarelli, F; Lyashuk, V; Mangano, S; Marcelin, M; Margiotta, A; Masullo, R; Mazeas, Florence; Mazure, A; Megna, R; Melissas, M; Migneco, E; Mongelli, M; Montaruli, T; Morganti, M; Moscoso, L; Motz, H; Musumeci, M; Naumann, C; Naumann Godo, M; Niess, V; Noble, A; Olivetto, C; Ostasch, R; Palanque Delabrouille, N; Payre, P; Peek, H; Perez, Amparo; Petta, C; Piattelli, P; Pillet, R; Pineau, J; Poinsignon, J; Popa, V; Pradier, T; Racca, C; Randazzo, N; Van Randwijk, J; Real, D; Regnier, M; Van Rens, B; Rethore, F; Rewiersma, P; Riccobene, G; Rigaud, Vincent; Ripani, M; Roca, V; Roda, C; Rolin, Jean-francois; Rostovtsev, A; Roux, Jean; Ruppi, M; Russo, G; Rusydi, G; Salesa, F; Salomon, K; Sapienza, P; Schmitt, F; Schuller, J; Shanidze, R; Sokalski, I; Spona, T; Spurio, M; Van Der Steenhoven, G; Stolarczyk, T; Streeb, K; Sulak, L; Taiuti, M; Tamburini, C; Tao, C; Tasca, L; Terreni, G; Urbano, F; Valdy, Pierre; Valente, V; Vallage, B; Vaudaine, G; Venekamp, G; Verlaat, B; Vernin, P; Van Wijk, R; Wijnker, G; Wobbe, G; De Wolf, E; Yao, A; Zaborov, D; Zaccone, H; Zornoza, J; Zuniga, J.
full-scale mechanical prototype line was deployed to a depth of 2500 m to test the leak tightness of the electronics containers and the pressure-resistant properties of an electromechanical cable under evaluation for use in the ANTARES deep-sea neutrino telescope. During a month-long immersion study, line parameter data were taken using miniature autonomous data loggers and shore-based optical time domain reflectometry. Details of the mechanical prototype line, the electromechanical cable and data acquisition are presented. Data taken during the immersion study revealed deficiencies in the pressure resistance of the electromechanical cable terminations at the entry points to the electronics containers. The improvements to the termination, which have been...
Tipo: Text
Palavras-chave: Sea deployment; Electromechanical cable; Optical time domain reflectometry; Leak detection; Underwater neutrino telescope; ANTARES.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/publication-3519.pdf

Quartz crystal microbalance, an efficient tool to design supramolecular structure on solid substrate
Provedor de dados: 5
Autores: Lazerges, Mathieu; Perrot, Hubert; Pailleret, Alain; Antoine, Elisabeth; Compere, Chantal.
Beyond interest of these step by step synthese of linear and branched DNA polymers, this work demonstrates that the quartz crystal microbalance is a sensitive tool to design supramolecular biostructures on solid substrate by following in situ thermodynamic and kinetics of successive reactions.
Tipo: Text
Ano: 2005
URL: http://archimer.ifremer.fr/doc/00089/19997/17660.pdf

Biofouling protection for marine underwater observatories sensors
Provedor de dados: 5
Autores: Delauney, Laurent; Compere, Chantal; Lehaitre, Michel.
These days, many marine autonomous environment monitoring networks are set up in the world. Such systems take advantage of existing superstructures such as offshore platforms, lightships, piers, breakwaters or are placed on specially designed buoys or deep sea fix stations. The major goal for these equipments is to provide in real time reliable measurements without costly frequent maintenance. These autonomous monitoring systems are affected by a well known phenomena in seawater condition which is biofouling. Consequently, such system without efficient biofouling protection is hopeless. This protection must be applied to the sensors and to the underwater acoustic communication equipments.
Tipo: Text
Ano: 2009
URL: http://archimer.ifremer.fr/doc/00107/21801/20176.pdf

Organometallic nanoprobe to enhance optical response on the polycyclic aromatic hydrocarbon benzo[a]pyrene immunoassay using SERS technology
Provedor de dados: 5
Autores: Dribek, Mohamed; Rinnert, Emmanuel; Colas, Florent; Crassous, Marie-pierre; Thioune, Nene; David, Catalina; De La Chapelle, Marc; Compere, Chantal.
We demonstrated the use of a new organometallic nanoprobe for competitive surface-enhanced Raman scattering (SERS) immunoassay devoted to the detection of polycyclic aromatic hydrocarbons (PAH) such as benzo[a]pyrene (BaP) in seawater. The nanoprobes are gold nanoparticles (GNPs) labeled by a Raman reporter, the 5,5'-dithiobis(succinimidyl-2-nitrobenzoate) (DSNB) and functionalized with monoclonal antibodies anti-BaP. The antibodies are bound with a high specificity to the analyte while the GNPs enhanced the Raman scattering of the DSNB. This type of immunoassay involved the grafting of BaP onto a sensing surface. Thus, NH2-terminated self-assembled monolayer is formed on the surface of gold substrate using cysteamine. Amines finally reacted with...
Tipo: Text
Palavras-chave: Biosensor; Surface-enhanced Raman scattering; Polycyclic aromatic hydrocarbons; Benzo[a]pyrene antibody; Organometallic nanoprobe.
Ano: 2017
URL: https://archimer.ifremer.fr/doc/00417/52844/53870.pdf

NOUBA Project Novel Natural Molecules Inhibiting the Development of Marine Bacteria in Biofilms
Provedor de dados: 5
Autores: Klein, Geraldine; Soum-soutera, Emmanuelle; Bazire, Alexis; Dreanno, Catherine; Compere, Chantal; Dufour, Alain.
Marine organisms represent a rather unexplored source of new activity and biological functions of molecules for biotechnologies. The research in chemical ecology of the marine environment leads to looking at metabolites of recognition or defence produced by these models. Marine bacteria belonging to the Pseudoalteromonas genus of the Gammaproteobacteria class are often found in association with marine eukaryotes, and their ability to produce a variety of biological activities attracted a particular attention. The marine Pseudoalteromonas sp. 3J6 and D41 were selected for their capacity to inhibit the biofilm formation of other bacteria. The study of antibiofilm metabolites synthesised by marine bacteria 3J6 and D41 biofilms can lead to the development of...
Tipo: Text
Ano: 2009
URL: http://archimer.ifremer.fr/doc/00089/19990/17666.pdf

Thiol- and Biotin-Labeled Probes for Oligonucleotide Quartz Crystal Microbalance Biosensors of Microalga Alexandrium Minutum
Provedor de dados: 5
Autores: Lazerges, Mathieu; Perrot, Hubert; Rabehagasoa, Niriniony; Compere, Chantal.
Two quartz crystal microbalance oligonucleotide biosensors of a toxic microalga gene sequence (Alexandrium Minutum) have been designed. Grafting on a gold surface of 20-base thiol- or biotin-labeled probe, and selective hybridization with the complementary 20-base target, have been monitored in situ with a 27 MHz quartz crystal microbalance under controlled hydrodynamic conditions. The frequency of the set up is stable to within a few hertz, corresponding to the nanogram scale, for three hour experiments. DNA recognition by the two biosensors is efficient and selective. Hybridization kinetic curves indicate that the biosensor designed with the thiol-labeled probe is more sensitive, and that the biosensor designed with the biotin-labeled probe has a shorter...
Tipo: Text
Palavras-chave: Alexandrium Minutum; Avidin; Biosensor; Biotin; DNA; Quartz crystal miocrobalance; Self-assembled monolayer; Thiol.
Ano: 2012
URL: http://archimer.ifremer.fr/doc/00107/21810/19385.pdf

Strategy to design DNA-biosensors: Single-stranded probe grafting versus target-probe duplex grafting
Provedor de dados: 5
Autores: Vedrine, C.; Lazerges, M.; Perrot, H.; Compere, Chantal; Pernelle, C.
A strategy to design DNA-biosensors by grafting probe-target duplex onto a 27 MHz quartz crystal microbalance gold surface is presented in this work. The idea that removing the DNA target after duplex grafting yields to a well accessible probe, and therefore to efficient target recognition, is investigated. Unfortunately, hybridization efficiency measured using such a straightaway protocol is equal to 15%, which is feeble by comparison with the 31% hybridization efficiency measured for biosensors designed with single-stranded probe. Successive DNA biosensors are designed in this work, taking into account phenomena that occur specifically in an interfacial environment, like non specific adsorption or exchange reactions between adsorbed molecules. A...
Tipo: Text
Palavras-chave: Alexandrium minutum; Biosensor; Double-stranded DNA; Quartz crystal microbalance.
Ano: 2012
URL: http://archimer.ifremer.fr/doc/00101/21196/19416.pdf

A biofilm-forming marine bacterium producing proteins as extracellular polymeric substances
Provedor de dados: 5
Autores: Leroy, Celine; Delbarre Ladrat, Christine; Ghillebaert, François; Compere, Chantal; Combes, Didier.
EPS biochemical characterization and observation by epifluorescencemicroscopy. Development of a marine biofilmformation model onto a polystyrene microtiterplate using the “O’Tooleand Koltermethod”[1] adapted with the well known fluorescent DNA staining DAPI
Tipo: Text
Ano: 2005
URL: http://archimer.ifremer.fr/doc/00089/19998/17661.pdf

Behaviour of stainless steel in natural seawater
Provedor de dados: 5
Autores: Compere, Chantal; Le Bozec, Nathalie.
In this paper, investigations performed in natural and artificial seawater on stainless steels will be presented. They concerned studies on: biofilm formation, passive layers composition, electrochemical behaviour, localised corrosion and the evolution of these different parameters as a function of ageing time. According to literature surveys, the different aspects will be discussed. Some conclusions will be drawn concerning the actual knowledge on the behaviour of stainless steels in seawater.
Tipo: Text
Ano: 1997
URL: http://archimer.ifremer.fr/doc/00089/19989/17653.pdf

Surface plasmon resonance in chalcogenide glass-based optical system
Provedor de dados: 5
Autores: Le Person, Jenny; Colas, Florent; Compere, Chantal; Lehaitre, Michel; Anne, M; Boussard Pledel, C; Bureau, B; Adam, J; Deputier, S; Guilloux Viry, M.
The surface plasmon resonance phenomenon has been studied in a chalcogenide glass-based optical system. IR transmission properties of these materials combined to their high refractive indices lead to advantageous properties for sensing. In this study, numerical simulations have been carried out to investigate the potentialities of sulfide glass from the GeGaSbS system as a coupling prism material. Then, an angular modulation SPR biosensor has been set up in the Kretschmann-Raether arrangement. Experimental data are consistent with numerical calculation and the detection limit of the sensor is 3 x 10(-5) RIU. These preliminary results are promising. Further investigations have to be carried out to confirm the great potentialities of those materials for...
Tipo: Text
Palavras-chave: Infrared; Biosensor; Surface plasmon resonance; Chalcogenide glass.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/publication-3955.pdf

Anti-rabbit immunoglobulin G detection in complex medium by PM-RAIRS and QCM Influence of the antibody immobilisation method
Provedor de dados: 5
Autores: Briand, Elisabeth; Salmain, M; Compere, Chantal; Pradier, C.
Two antibody immobilisation procedures were compared to set up an immunosensor for goat anti-rabbit immunoglobulin (anti-rIgG), i.e. rIgG covalently bound or immobilised via affinity to protein A (PrA). In both cases, the first layer of protein was covalently bound to a mixed self-assembled monolayer (SAM) of mercaptoundecanoic acid (MUA) and mercaptohexanol (C6OH) on a gold surface. The elaboration of the sensitive surfaces, as well as their selectivity and sensitivity were studied step by step by polarization modulation-reflection absorption infra-red spectroscopy (PM-RAIRS) and quartz crystal trticrobalance (QCM) with impedance measurement. QCM measurements showed that the viscoelastic properties of the antibody layer were markedly modified during the...
Tipo: Text
Palavras-chave: RIgG immobilisation; PM RAIRS; QCM; Immunosensors.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/publication-3580.pdf

RF sputtered amorphous chalcogenide thin films for surface enhanced infrared absorption spectroscopy
Provedor de dados: 5
Autores: Verger, F.; Nazabal, V.; Colas, Florent; Nemec, P.; Cardinaud, C.; Baudet, E.; Chahal, R.; Rinnert, Emmanuel; Boukerma, Kada; Peron, Isabelle; Deputier, S.; Guilloux-viry, M.; Guin, J. P.; Lhermite, H.; Moreac, A.; Compere, Chantal; Bureau, Bruno.
The primary objective of this study is the development of transparent thin film materials in the IR enabling strong infrared absorption of organic compounds in the vicinity of metal nanoparticles by the surface plasmon effect. For developing these optical micro-sensors, hetero-structures combining gold nanoparticles and chalcogenide planar waveguides are fabricated and adequately characterized. Single As2S3 and Ge25Sb10Se65 amorphous chalcogenide thin films are prepared by radio-frequency magnetron sputtering. For the fabrication of gold nanoparticles on a chalcogenide planar waveguide, direct current sputtering is employed. Fabricated single layers or hetero-structures are characterized using various techniques to investigate the influence of deposition...
Tipo: Text
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00170/28094/26679.pdf

Proteomic approaches applied to adhesion factors in marine biofilm-forming bacteria
Provedor de dados: 5
Autores: Ritter, Andres; Com, Emmanuelle; Bazire, Alexis; Dos Santos Goncalves, Marina; Pineau, Charles; Compere, Chantal; Dufour, Alain; Potin, Philippe.
Biofouling is ubiquitous in marine environment, and bacteria are among the first organisms to foul surfaces. They form biofilms which serve as focus for the attachment and growth of other organisms, such as invertebrates, sessile plants, and animals (Davis et al., 1989). Mature marine biofouling communities are complex, highly dynamic ecosystems (Fig.1) and once established are extremely difficult to eradicate (Holmstrom et al., 2002). For this reason the understanding of the mechanisms leading to marine bacterial attachment and its subsequent biofilm development are of great biological importance with obvious potential industrial outcomes. This development is conditioned by complex processes involving bacterial attachment to surfaces, growth, cell-to-cell...
Tipo: Text
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00089/19987/17651.pdf

Monoclonal antibody against the surface of alexandrium minutum used in a whole-cell ELISA (Poster)
Provedor de dados: 5
Autores: Gas, Fabienne; Pinto, Laetitia; Baus, Beatrice; Gaufres, Laure; Crassous, Marie-pierre; Compere, Chantal; Quemeneur, Eric.
PRODUCTION AND CHARACTERIZATION OF MONOCLONAL ANTIBODY ANTI-Alexandrium minutum
Tipo: Text
Ano: 2008
URL: http://archimer.ifremer.fr/doc/00089/19994/17657.pdf

Biosensors for the detection and monitoring of Alexandrium minutum along the French coasts
Provedor de dados: 5
Autores: Antoine, Elisabeth; Compere, Chantal; Cosnier, Serge; Crassous, Marie-pierre; Derbal, Hassina; Erard-le Denn, Evelyne; Gondran, Chantal; Guilminot, Elodie; Lehaitre, Michel; Mazeas, Florence; Moreau, Hervé; Perrot, Hubert; Pradier, Claire-marie; Salmain, Michèle.
The microalgae Alexandrium minutum produces neurotoxins responsible for paralytic shellfish poisoning, which upon accumulation in shellfish represent a human health risk. This species recurrently forms toxic blooms in the Northern part of the French Brittany coast. At present, algae identification relies on tedious microscope observations mostly performed by skilled taxonomists in laboratories. Newly emerging fields of molecular taxonomy, nanoscale technology and biotechnology offer opportunities for the development of in-situ biosensors for the detection and the monitoring of toxic algae.
Tipo: Text
Ano: 2003
URL: http://archimer.ifremer.fr/doc/00089/19986/17650.pdf

Organometallic nanoprobe to enhance optical response on Polycyclic Aromatic Hydrocarbons (PAHs) immunoassay using SERS technology
Provedor de dados: 5
Autores: Dribek, Mohamed; Rinnert, Emmanuel; Colas, Florent; Crassous, Marie-pierre; Compere, Chantal.
We demonstrate the possible use of organometallic nanoprobe on competitive surface-enhanced Raman scattering immunoassay for policyclic aromatic hydrocarbon detection in sea water. This type of SERS immunoassay is based on to capture Raman reporter-labeled immuno-Au onto the immobilized layer of antigens (benzo[a]pyrene) on the gold substrate after incubation of these nanoprobes with PAHs including in the sample. To realize nanoprobes, first we have prepared gold nanoparticles (GNPs) according to Frens’ method. Then, we have labeled GNPs by the Raman reporter of 5,5’-Dithiobis(succinimidyl-2-nitrobenzoate) (DSNB). Thereafter, Benzo[a]pyrene antibody was attached to the DSNB labeled GNPs. Before use in SERS detection, their activity was controlled by...
Tipo: Text
Ano: 2012
URL: http://archimer.ifremer.fr/doc/00088/19912/17576.pdf

One step immunochromatographic assay for the rapid detection of Alexandrium minutum
Provedor de dados: 5
Autores: Gas, Fabienne; Baus, Beatrice; Pinto, Laetitia; Compere, Chantal; Tanchou, Valerie; Quemeneur, Eric.
Harmful algal blooms represent a major threat to marine production, and particularly to shellfish farming. Current methods for analyzing environmental samples are tedious and time consuming because they require taxonomists and animal experiments. New rapid detection methods, such as immunoassays, are sought for alerting purposes and for the study of algal ecodynamics in their natural environment. Alexandrium minutum, which causes paralytic shellfish poisoning, occurs with increasing frequency along European coasts. We have developed a one step immunochromatographic assay which is based on the principle of immunochromatographic analysis and involves the use of two distinct monoclonal antibodies directed against surface antigens of A. minutum. The primary...
Tipo: Text
Palavras-chave: Antibody; Alexandrium minutum; Immunochromatographic assay (ICA); Rapid detection.
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00000/11153/7819.pdf

A marine bacterial adhesion microplate test using the DAPI fluorescent dye: a new method to screen antifouling agents
Provedor de dados: 5
Autores: Leroy, Celine; Delbarre Ladrat, Christine; Ghillebaert, F; Rochet, Marie-joelle; Compere, Chantal; Combes, D.
Aims: To develop a method to screen antifouling agents against marine bacterial adhesion as a sensitive, rapid, quantitative microplate fluorescent test.  Methods and Results: Our experimental method is based on a natural biofilm formed by mono-incubation of the marine bacterium Pseudoalteromonas sp. D41 in sterile natural sea water in a 96 wells polystyrene microplate. The 4'6-diamidino-2-phenylindole (DAPI) dye was used to quantify adhered bacteria in each well. Total measured fluorescence in wells was correlated with the amount of bacteria showing a detection limit of one bacterium per 5 µm2 and quantifying 2 x 107 to 2 x 108 bacteria adhered per cm2. The antifouling properties of three commercial surface active agents and chlorine were tested by this...
Tipo: Text
Palavras-chave: Antifouling; Pseudoalteromonas; Screening; Microtiter plate; Adhesion; DAPI; Marine biofilm.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2006/publication-2017.pdf

Influence of subtilisin on the adhesion of a marine bacterium which produces mainly proteins as extracellular polymers
Provedor de dados: 5
Autores: Leroy, Celine; Delbarre Ladrat, Christine; Ghillebaert, F; Compere, Chantal; Combes, D.
Aims: The nature of exopolymers involved in the adhesion of a marine biofilm-forming bacterium Pseudoalteromonas sp. D41 was investigated to evaluate and understand the antifouling potential of subtilisin.   Methods and Results: The exopolymers of D41 produced by fermentation were analysed by FTIR and SDS-PAGE showing the presence of polysaccharides, glycoproteins and proteins. A high content of proteins was detected both in soluble and capsular fractions. The microscopic observations of fluorescamine and calcofluor stained adhered D41 indicated mainly the presence of proteins in exopolymers produced during adhesion. Subtilisin, the broad spectrum protease, tested in natural sea water and in polystyrene microplates showed that antifouling activity was...
Tipo: Text
Palavras-chave: Subtilisin; Pseudoalteromonas; Exopolymer; Capsular protein; Biofilm; Antifouling.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/publication-4624.pdf

Direct and fast detection of Alexandrium minutum algae by using high frequency microbalance
Provedor de dados: 5
Autores: Sousa, Celia; Compere, Chantal; Dreanno, Catherine; Crassous, Marie-pierre; Gas, Fabienne; Baus, Beatrice; Perrot, Hubert.
In this paper, a simple detection of a toxic algae, Alexandrium minutum, was developed using highly sensitive quartz crystal microbalance. In terms of performance, compared with other conventional analytical tools, the main interest of our immunosensor is based on a fast and direct detection of these living cells. This system requires the use of one monoclonal antibody directed against the surface antigen of A. minutum. We demonstrate that the whole living and motile algae are caught and detected. The high specificity of the biosensor is also demonstrated by testing several other dinoflagellate species. The frequency shift is correlated to the A. minutum cells concentration. This simple system is potentially promising for environmental monitoring purposes.
Tipo: Text
Palavras-chave: Alexandrium minutum; Quartz microbalance; Microalgae; Immunosensor.
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00194/30492/28897.pdf

Development of environmentally friendly antifouling paints using biodegradable polymer and lower toxic substances
Provedor de dados: 5
Autores: Carteau, David; Vallee-rehel, Karine; Linossier, Isabelle; Quiniou, Francoise; Davy, Romain; Compere, Chantal; Delbury, Maxime; Fay, Fabienne.
The development of new antifouling coatings with respect to the marine environment is actually crucial. The aim of the present work is to concept an erodible paint formulated with biodegradable polyester as binders and which combines two modes of prevention: chemical and physical repelling of biofouling. This system is principally dedicated to disturb durable settlement of microfouling. Each component was chosen according to its specific properties: chlorhexidine is a bisdiguanide antiseptic with antibacterial activity, zinc peroxide is an inorganic precursor of high instable entities which react with seawater to create hydrogen peroxide, Tween 85 is a non ionic surfactant disturbing interactions between colonizing organisms and surface. Obtained results...
Tipo: Text
Palavras-chave: Environmental coatings; Antifouling; Active substances; Microfouling.
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00168/27923/27273.pdf

Direct detection of toxic micro-algae Alexandrium minutum through high sensitive gravimetric sensors
Provedor de dados: 5
Autores: Bucur, Bogdan; Lazerges, Mathieu; Perrot, Hubert; Antoine, Elisabeth; Hamelin, Muriel; Compere, Chantal.
In this work QCM technologies were used to set up a DNA based biosensor dedicated to the detection of toxic micro-algae in the environment. Up to now, appropriate detection were not achieved for different reasons as the possibility of regeneration, the capability of multidetection or the detection of very low DNA target concentration. For these reasons different configurations were examined based on in-situ and fast regeneration, supramolecular chemistry of DNA and high sensitive gravimetric devices. In a first step an OligoDesoxyRibonucleotide 5’ tailed with a disulfide group (SH-ODN) designed to be a specific molecular probe for the toxic micro-algae Alexandrium minutum was grafted on the quartz surface resonators. Kinetics of probe immobilization and of...
Tipo: Text
Ano: 2008
URL: http://archimer.ifremer.fr/doc/00089/19996/17659.pdf

Kinetics of conditioning layer formation on stainless steel
Provedor de dados: 5
Autores: Compere, Chantal; Bellon Fontaine, M.-n.; Bertrand, P; Costa, D; Marcus, P; Poleunis, C; Pradier, C.m.; Rondot, B; Walls, M.g.
Adhesion of micro-organisms to surfaces in marine environments leads to biofouling. The deleterious effects of biofilm growth in the marine environment are numerous and include : energy losses due to increased fluid frictional resistance or to increased heat transfer resistance, risk of corrosion induced by micro-organisms, loss of optical properties, quality control and safety problems. Antifouling agents are generally used to protect surfaces from being affected by such a biofilm. These agents are toxic and can be persistent, causing harmful environmental and ecological effects. Moreover, the use of biocides and regular cleaning considerably increase the maintenance costs of marine industries. An improved knowledge of the biofilm adhesion mechanisms is...
Tipo: Text
Palavras-chave: Biofilm; Surface characterisation; Carbohydrates; Protein; Seawater; Conditioning layer.
Ano: 2001
URL: http://archimer.ifremer.fr/doc/2001/publication-1701.pdf

A Biosensor for Detection of DNA Sequences Based on a 50MHz QCM electronic oscillator circuit
Provedor de dados: 5
Autores: Bustabad, E. A.; Garcia, G; Rodriguez-pardo, L.; Farina, J.; Perrot, H.; Gabrielli, C.; Bucur, B.; Lazerges, M.; Rose, D.; Compere, Chantal; Arnau, A.
This work deals with the development of a high sensibility DNA biosensor based on a 50MHz Quartz Crystal Microbalance (QCM) Oscillator Electronic Circuit. The designed QCM oscillator sensor is able to detect the presence of complementary DNAs in a solution that match the sequence on a given strand in function of the changes in the output frequency of the oscillator. The design is adapted so that the Barkhausen condition is satisfied even when the quartz is immerged in liquid media. Also a comparative study of the developed 50MHz biosensor in front of a QCM oscillator with smaller frequency is carried out, with object of checking if the sensibility of the oscillator increases, allowing to detect smaller concentrations of the complementary DNA.
Tipo: Text
Ano: 2009
URL: http://archimer.ifremer.fr/doc/00021/13198/10574.pdf

Organisation structurale et caractérisation biochimique des biofilms sur alliages inoxydables
Provedor de dados: 5
Autores: Salaun, Stephanie; Rinnert, Emmanuel; Compere, Chantal.
Cette étude s’inscrit dans le cadre du projet REI n°2010 34 0010 intitulé « Biofilms et corrosion d’alliages inoxydables en eau de mer », et a pour objectif de caractériser le biofilm responsable de l’activation de la corrosion des alliages inoxydables en eau de mer par une approche pluridisciplinaire, dans le but d’identifier les composantes responsables de ce type de corrosion. Ce biofilm spécifique est ainsi en cours de caractérisation par des méthodes de microscopie Apotome et de spectroscopie Raman. Ce poster a fait l'objet d'une présentation lors de l'Ecole thématique du CNRS : Voyage au centre d'un biofilm : physico-chimie des exopolymères de l'espace inter-cellulaire. Nancy, les 27 et 29 septembre 2011.
Tipo: Text
Ano: 2011
URL: http://archimer.ifremer.fr/doc/00057/16775/14228.pdf

Layer-by-Layer DNA film synthesis via branched hybridization
Provedor de dados: 5
Autores: Lazerges, M.; Perrot, H.; Antoine, Elisabeth; Compere, Chantal.
A new Layer-by-Layer (LbL) DNA film synthesis, where the driving force is the natural DNA hybridization, is presented in this work. The DNA films were synthesized via a branched mechanism and do not include any chemical binders between each layer as it is the case for film obtained by other synthesis paths. Kinetics of film formation were monitored by mass measurements with an electroacoustic network analyzer. The presented synthesis is a pathway to new DNA structures which can be used in biotechnologies and is complementary to those obtained by other LbL techniques.
Tipo: Text
Palavras-chave: LbL; Electroacoustic; DNA film; Multicouche; Film ADN; Électroacoustique.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/publication-6217.pdf

Quantitative SERS sensors for environmental analysis of naphthalene
Provedor de dados: 5
Autores: Peron, Olivier; Rinnert, Emmanuel; Toury, T.; De La Chapelle, M. Lamy; Compere, Chantal.
In the investigation of chemical pollutants, such as PAHs (Polycyclic Aromatic Hydrocarbons) at low concentration in aqueous medium, Surface-Enhanced Raman Scattering (SERS) stands for an alternative to the inherent low cross-section of normal Raman scattering. Indeed, SERS is a very sensitive spectroscopic technique due to the excitation of the surface plasmon modes of the nanostructured metallic film. The surface of quartz substrates was coated with a hydrophobic film obtained by silanization and subsequently reacted with polystyrene (PS) beads coated with gold nanoparticles. The hydrophobic surface of the SERS substrates pre-concentrates non-polar molecules such as naphthalene. Under laser excitation, the SERS-active substrates allow the detection and...
Tipo: Text
Ano: 2011
URL: http://archimer.ifremer.fr/doc/00026/13760/10890.pdf

First steps of biofilm formation on stainless steels in natural seawater
Provedor de dados: 5
Autores: Bellon-fontaine, M.-n.; Bertrand, P; Compere, Chantal; Costa, D; Guezennec, Jean; Marcus, P; Poleunis, C.; Pradier, C.-m.; Rondot, B; Walls, M.g.
Numerous works are reported in literature concerning the biofilm growth on surfaces in seawater but the understanding of the adhesion mechanisms in the first steps of immersion is still subject to investigations. A knowledge of the chemical composition and of the kinetics of conditioning layer formation is a central requirement for a sound scientific understanding of biofilm growth. Due to its complexity and to the interdisciplinary nature of the subject, this research asks for the collaboration between the fields of material science, surface analyses, chemistry, marine microbiology and biochemistry. The first steps of biofilm formation on 316L stainless steels immersed in natural sea water have been studied, using a broad range of surface characterisation...
Tipo: Text
Palavras-chave: Sea water; Substrates; Biofilm.
Ano: 1998
URL: http://archimer.ifremer.fr/doc/1998/acte-1699.pdf

45-and 70-base DNA supramolecular polymerizations on quartz crystal microbalance biosensor
Provedor de dados: 5
Autores: Lazerges, Mathieu; Perrot, Hubert; Rabehagasoa, Niriniony; Antoine, Elisabeth; Compere, Chantal.
Supramolecular polymerizations of 45- and 70- base DNAs on the surface of an in-situ time-resolved 27 MHz quartz crystal microbalance biosensor.
Tipo: Text
Palavras-chave: Sensor; Design; Kinetics; Immobilization; Hybridization.
Ano: 2005
URL: http://archimer.ifremer.fr/doc/2005/publication-1710.pdf

In situ measurement with diffusive gradients in thin films: effect of biofouling in freshwater
Provedor de dados: 5
Autores: Uher, Emmanuelle; Compere, Chantal; Combe, Matthieu; Mazeas, Florence; Gourlay-france, Catherine.
Concerning in situ passive sampler deployment, several technical priorities must be considered. In particular, deployment time must be sufficiently long not only to allow a significant quantity to be accumulated to facilitate analysis but also to ensure that the signal is above the quantification limit and out of the blank influence. Moreover, regarding the diffusive gradient in thin films (DGT) technique, deployment time must also be sufficiently long (at least 5 days) to avoid the interactions of the solutes with the material diffusion layer of the DGT and for the steady state to be reached in the gel. However, biofouling occurs in situ and modifies the surface of the samplers. In this article, we propose a kinetic model which highlights the biofouling...
Tipo: Text
Palavras-chave: DGT; Metals; Biofouling; Seine River; Field deployment; Passive sampler.
Ano: 2017
URL: http://archimer.ifremer.fr/doc/00382/49330/49759.pdf

In situ QCM DNA-biosensor probe modification
Provedor de dados: 5
Autores: Lazerges M, M; Perrot, H; Zeghib N, N; Antoine, Elisabeth; Compere, Chantal.
We report on a probe modification of a quartz crystal microbalance (QCM) DNA-biosensor that permits to reversibly change the DNA sequence detected. A QCM DNA-biosensor was designed by immobilization of a 20-base DNA-disulfide probe on the gold-covered quartz surface of a 27 MHz microbalance (9 MHz, third overtone). After immobilization on the gold covered quartz surface, this probe was modified by hybridization with a 45-base DNA that includes the complementary 20-base sequence, a 5-base spacer and a non-complementary 20-base sequence. The non-complementary sequence constitutes a new probe, different from the DNA-disulfide probe, that permits the detection of a new DNA target. As this 45 bases DNA is changeable by dehybridization, successive different DNA...
Tipo: Text
Palavras-chave: QCM; DNA; Biosensor.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/publication-1985.pdf

New Approach For The Detection Of Toxic Algae Species
Provedor de dados: 5
Autores: Colas, Florent; Crassous, Marie-pierre; Laurent, Sebastien; Dreanno, Catherine; Compere, Chantal.
Up to now, toxic species are monitored by expensive and time-consuming laboratory analysis of seawater samples: after a 24h decantation, the samples are observed with an optical microscope and the cell concentration per species is determined. In this context, systems able to detect HAB species directly underwater would be of great help. An assay based on an SPR biosensor was then developed for detecting Alexandrium minutum.
Tipo: Text
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00089/19995/17658.pdf

First Steps of in Situ Surface-Enhanced Raman Scattering During Shipboard Experiments
Provedor de dados: 5
Autores: Peron, Olivier; Rinnert, Emmanuel; Colas, Florent; Lehaitre, Michel; Compere, Chantal.
It is shown that the surface-enhanced Raman scattering (SERS) technique can be applied to detect organic molecules during in situ experiments. To this purpose, we used trans-1,2-bis(4-pyridyl)ethylene (BPE) as a target molecule. Adsorbed on the SERS chemosensor surface and excited under laser, the vibration modes of the molecules can be identified. SERS chemosensors are based on quartz substrates functionalized by silanization and partially coated with gold nanoparticles. SERS measurements during shipboard experiments were made with a home-made in situ Raman spectrometer connected to a marinized micro-fluidic system. The device was designed to host chemosensors in order to ensure measurements with a flow cell. A theoretical limit of detection was estimated...
Tipo: Text
Palavras-chave: Surface-enhanced Raman scattering; SERS; Chemosensors; Flow cells; Shipboard experiments; In situ measurements; Freundlich isotherm.
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00016/12691/9718.pdf

IR-biosensor for in situ detection of toxic algae alexandrium minutum
Provedor de dados: 5
Autores: Le Person, Jenny; Compere, Chantal; Lehaitre, Michel; Anne, Marie-laure; Boussard-pledel, Catherine; Bureau, Bruno; Adam, Jean-luc; Guilloux-viry, M; Burel, L.
Toxic algal blooms are a public health issue and constitute a menace for coastal areas. The potentiality of a chalcogenide optical fibre biosensor, operating in the mid-infrared (MIR) spectral domain, is studied. In order to monitor blooms of toxic algae, more specifically Alexandrium minutum specie, two ways are explored: immuno-biosensing and DNA identification.
Tipo: Text
Ano: 2005
URL: http://archimer.ifremer.fr/doc/00089/19993/17656.pdf

Towards in situ detection of PAH trace in sea-water using SERS-active sensors
Provedor de dados: 5
Autores: Peron, Olivier; Rinnert, Emmanuel; Lehaitre, Michel; Colas, Florent; Compere, Chantal.
This paper reports the development of a sensor based on surface-enhanced Raman scattering (SERS) for analyses in sea-water. Polycyclic aromatic hydrocarbons (PAHs) are targeted by these sensors and their detection in situ summons up chemical synthesis and optical development. Firstly, a relevant synthesis of SERS active substrates based on gold nanostructures is presented. Different kinds of substrates have been synthesized under variable experimental conditions to modify some parameters such as i) gold shape, size and distribution and such as ii) chemical functionalization: (i) gold nanoparticles were prepared either by chemical reduction of HAuCl4 or by physical deposition. (ii) Substrates were functionalized by hydrophobic films to allow nonpolar...
Tipo: Text
Palavras-chave: Functionalization; Silanization; Gold nanostructures; SERS; PAH detection; Sea-water; Sensor; In situ.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/00012/12363/9152.pdf

The effect of the salinity level on conductivity sensor calibration
Provedor de dados: 5
Autores: Le Bihan, Caroline; Salvetat, Florence; Lamande, Nolwenn; Compere, Chantal.
This paper presents a study on the calibration of conductivity sensors. The puropse is to demonstrate the effect of the salinity concentration on the response of conductivity sensor. Several experiments are performed on three sensors of different technologies: a Seabird Sbe37-SIP MicroCAT, a Falmouth Scientific Inc. IMCTD-MBP-D and a NKE Instrumentation Smatch. The analysis of the results shows an overall impact of the salinity level on the sensor response. This effect is discussed regarding the oceanographic requirements.
Tipo: Text
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00260/37143/35668.pdf