Consulta: creatorFacets:"Conceição,Rita de Cássia dos Santos da"
Registros recuperados: 3
Data/hora: 01/06/2024 06:19:14

Detection of salmonella sp in chicken cuts using immunomagnetic separation
Provedor de dados: 58
Autores: Conceição,Rita de Cássia dos Santos da; Moreira,Ângela Nunes; Ramos,Roberta Juliano; Goularte,Fabiana Lemos; Carvalhal,José Beiro; Aleixo,José Antonio Guimarães.
The immunomagnetic separation (IMS) is a technique that has been used to increase sensitivity and specificity and to decrease the time required for detection of Salmonella in foods through different methodologies. In this work we report on the development of a method for detection of Salmonella in chicken cuts using in house antibody-sensitized microspheres associated to conventional plating in selective agar (IMS-plating). First, protein A-coated microspheres were sensitized with polyclonal antibodies against lipopolysacharide and flagella from salmonellae and used to standardize a procedure for capturing Salmonella Enteritidis from pure cultures and detection in selective agar. Subsequently, samples of chicken meat experimentally contaminated with S....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Salmonella; Immunomagnetic separation; Rapid methods.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000100034

Biofilm formation by Vibrio parahaemolyticus on different surfaces and its resistance to sodium hypochlorite
Provedor de dados: 65
Autores: Rosa,Janaina Viana da; Conceição,Natália Volpato da; Conceição,Rita de Cássia dos Santos da; Timm,Cláudio Dias.
ABSTRACT:  Vibrio parahaemolyticus is an important pathogen for both fish industry and consumers. It forms biofilm which makes it difficult to eliminate this microorganism using sanitizers. This study aimed to assess biofilm formation on different surfaces and effect of biofilm on resistance to sanitizers. Eight isolates of biofilm-forming V. parahaemolyticus were tested for the ability to form biofilms on a number of surfaces including high density polyethylene, stainless steel, glass, exoskeleton of Farfantepenaeus paulensis (Pink Shrimp), and operculum of Micropogonias furnieri (Whitemouth Croaker). Efficiency of sanitizer sodium hypochlorite against the bacteria was evaluated in the biofilms formed on the surface of the materials used; out the eight...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bacterial contamination; Sanitizers; Food safety; Fish; Shrimp.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018001200451

Inducers and autoinducers on Salmonella enterica serovar Typhimurium motility, growth and gene expression
Provedor de dados: 65
Autores: Conceição,Rita de Cássia dos Santos da; Sturbelle,Régis Tuchtenhagen; Timm,Cláudio Dias; Leite,Fábio Pereira Leivas.
Genus Salmonella bacteria are among the major pathogenic microorganisms in food. This bacterium pathogenicity is related to a number of virulence factors, among which its flagella. Flagellum expression is one of the virulence factors modulated by Quorum Sensing. Epinephrine produced by mammals uses the same signaling pathway of the 3 bacteria autoinducer. This study evaluated the effect of molecules inducer (epinephrine) and autoinducers (autoinducer 2 and autoinducer 3) and their association with the motility, growth and expression genes flhC, fliA, fliY, motA, motB e fliC of Salmonella Typhimurium (ST). Initially, ST was inoculated in BHI. Then, motility assays, growth curves and gene expression were performed by testing different concentrations of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Quorum sensing; Salmonella; Epinephrine; Autoinducer; Motility.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782015001202201