Consulta: creatorFacets:"Consoli,Luciano"
Registros recuperados: 6
Data/hora: 11/06/2024 02:12:09

Assessment of genetic diversity in Brazilian barley using SSR markers
Provedor de dados: 74
Autores: Ferreira,Jéssica Rosset; Pereira,Jorge Fernando; Turchetto,Caroline; Minella,Euclydes; Consoli,Luciano; Delatorre,Carla Andréa.
Abstract Barley is a major cereal grown widely and used in several food products, beverage production and animal fodder. Genetic diversity is a key component in breeding programs. We have analyzed the genetic diversity of barley accessions using microsatellite markers. The accessions were composed of wild and domesticated barley representing genotypes from six countries and three breeding programs in Brazil. A total of 280 alleles were detected, 36 unique to Brazilian barley. The marker Bmag120 showed the greatest polymorphism information content (PIC), with the highest mean value found on chromosome three, and the lowest on chromosomes four and six. The wild accessions presented the highest diversity followed by the foreign genotypes. Genetic analysis was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Domesticated barley; Genetic diversity; Hordeum vulgare ssp. vulgare; Hordeum vulgare ssp. spontaneum; Microsatellite markers.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000100086

Gene expression analysis reveals important pathways for drought response in leaves and roots of a wheat cultivar adapted to rainfed cropping in the Cerrado biome
Provedor de dados: 74
Autores: Poersch-Bortolon,Liane Balvedi; Pereira,Jorge Fernando; Nhani Junior,Antonio; Gonzáles,Hebert Hernán Soto; Torres,Gisele Abigail Montan; Consoli,Luciano; Arenhart,Rafael Augusto; Bodanese-Zanettini,Maria Helena; Margis-Pinheiro,Márcia.
Abstract Drought limits wheat production in the Brazilian Cerrado biome. In order to search for candidate genes associated to the response to water deficit, we analyzed the gene expression profiles, under severe drought stress, in roots and leaves of the cultivar MGS1 Aliança, a well-adapted cultivar to the Cerrado. A set of 4,422 candidate genes was found in roots and leaves. The number of down-regulated transcripts in roots was higher than the up-regulated transcripts, while the opposite occurred in leaves. The number of common transcripts between the two tissues was 1,249, while 2,124 were specific to roots and 1,049 specific to leaves. Quantitative RT-PCR analysis revealed a 0.78 correlation with the expression data. The candidate genes were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: 454 sequencing; Candidate genes; RT-qPCR; Triticum aestivum; Water deficit.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000400629

Glutenin analysis for the safe screening of self-pollinated wheat seeds when developing doubled-haploid populations
Provedor de dados: 86
Autores: Torres,Gisele Abigail Montan; Scagliusi,Sandra Maria Mansur; Chaves,Márcia Soares; Consoli,Luciano.
Abstract: The objective of this work was to apply glutenin analysis, as a simple and reliable method, to identify self-pollinated wheat (Triticum aestivum) seeds in F1 crosses, when developing mapping populations using doubled-haploid (DH) technology. F1 seeds were subjected to glutenin analyses using gel electrophoresis, to verify and confirm their heterozygotic profiles. Glutenin analyses were carried out using one half of the endosperm of each of the 87 F1 seeds. The other half of the endosperm was used to develop the mapping population through DHs. Plants with band patterns different from the expected ones were discarded. Although the incidence of self-pollinated seeds was relatively low (only 8%), the screening of F1 heterozygotic profiles is...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Puccinia recondita f. sp. tritici; Triticum aestivum; Adult plant resistance; Leaf rust; Microspore culture; Toropi wheat cultivar.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2018000500646

Androgenic response of wheat genotypes resistant to fusariosis
Provedor de dados: 86
Autores: Coelho,Mônica Bossardi; Scagliusi,Sandra Maria Mansur; Lima,Maria Imaculada Pontes Moreira; Consoli,Luciano; Grando,Magali Ferrari.
Abstract: The objective of this work was to assess the androgenic response, via microspore culture, of wheat genotypes with different levels of resistance to Gibberella zeae. The number of androgenic embryos per spike, and of green and albino plants was counted for the BRS 179 (moderately resistant), Frontana and Sumai 3 (resistant), and BRS 194, Embrapa 27, and Fielder (susceptible) genotypes. The degree of interference by the Fielder, Pavon 76, and Sumai 3 ovary-donor genotypes, used for co-culture with the microspore cells, was also assessed regarding androgenic response. Induction efficiency ranged from 0.33 embryo per spike for Embrapa 27 to 109.8 embryos for Frontana. Sumai 3 presented the second best response, and Embrapa 27 behaved as highly...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gibberella zeae; Triticum aestivum; Doubled haploids; Microspore.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2018000500575

Impact of high-molecular-weight glutenin alleles on wheat technological quality
Provedor de dados: 86
Autores: Vancini,Camila; Torres,Gisele Abigail Montan; Miranda,Martha Zavariz de; Consoli,Luciano; Bonow,Sandro; Grando,Magali Ferrari.
Abstract: The objective of this work was to determine high-molecular-weight glutenin subunits (HMW-GS) and their relationship with technological quality parameters in a collection of wheat (Triticum aestivum) genotypes. Two hundred and seventy-two accessions were evaluated on SDS-Page, and molecular markers were used to identify the 7oe allele and 1BL.1RS translocation. For 219 accessions with a homogenous glutenin profile, 53 profiles and 21 alleles were identified. The most frequent combination was 2*/7+9/5+10 (11.9%). The mean value of genetic diversity for the three assessed Glu-1 loci was 0.67. Based on the HMW-GS profile and on the presence of the 1BL.1RS translocation, the Glu-1 score was calculated and its correlation with technological quality...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Triticum aestivum; Bread-making quality; Glu-1 score; Gluten strength; HMW-GS.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2019000102801

Dissecting the Opaque-2 regulatory network using transcriptome and proteome approaches along with enzyme activity measurements
Provedor de dados: 63
Autores: Lefèvre,Agnès; Consoli,Luciano; Gaziola,Salete A.; Pellegrino,Ana Paula; Azevedo,Ricardo A.; Damerval,Catherine.
The Opaque-2 (O2) gene encodes a transcriptional activator specifically expressed for grain development of maize. o2 mutants have an opaque and chalky kernel, with a decrease in zein storage protein content, and an increase in the proportions of lysine and tryptophan. In this review, we present recent results investigating genetic properties of the O2 network, using transcriptome and proteome approaches, associated with measurements of activities of enzymes of the aspartate pathway and lysine degradation. The structural polymorphism at the O2 locus was investigated by RFLP in a collection of 51 maize inbred lines. Most polymorphic sites were found outside the coding regions. We then searched for relationships between RFLP polymorphism and (i) mRNA...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Zein; Aspartate kinase; Lysine oxoglutarate reductase/saccharopine dehydrogenase; Polymorphism.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162002000200031