Consulta: creatorFacets:"Corrêa,F.N."
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Development and standardization of a western blotting test for detection of antibodies against B. abortus
Provedor de dados: 45
Autores: Falcão,M.V.D.; Santana,V.L.A.; Corrêa,F.N.; Tenório,J.A. B.; Mota,R.A.
ABSTRACT Brucellosis is an infectious disease caused by bacteria of the genus Brucella spp. with diagnosis based on use of serological techniques. The present study aimed to develop and standardize a western blotting (WB) test for detection of antibodies against B. abortus. Samples from two groups of cattle were analyzed: group I: 60 serum samples from true positive and true negative vaccinated animals (30 positive samples from infected animals according to rose bengal test (RBT), 2-mercaptoethanol, serum agglutination test (SAT) and complement fixation test (CFT) and 30 RBT negatives samples); group II: 383 field samples (90 positive and 293 CFT negative sera). The most reactive band in the western blotting, which properly identified and separated...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Brucellosis; Immunodiagnosis; Proteomics; Serology.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000100160