Consulta: creatorFacets:"Cueff-gauchard, Valerie"
Registros recuperados: 15
Data/hora: 06/06/2024 09:26:50

Gill chamber and gut microbial communities of the hydrothermal shrimp Rimicaris chacei Williams and Rona 1986: A possible symbiosis
Provedor de dados: 5
Autores: Apremont, Vincent; Cambon-bonavita, Marie-anne; Cueff-gauchard, Valerie; Francois, David; Pradillon, Florence; Corbari, Laure; Zbinden, Magali.
Rimicaris chacei Williams and Rona 1986, formerly named as Chorocaris chacei, is a caridean shrimp living in deep-sea hydrothermal ecosystems. This shrimp is endemic to the Mid Atlantic Ridge (MAR) and lives at the periphery of aggregates of its well-known congeneric R. exoculata Williams and Rona 1986. Contrasting with the very dense and mobile clusters formed by R. exoculata, R. chacei lives in small groups of several individuals that are not very mobile. Although devoid of the characteristic hypertrophied cephalothorax of R. exoculata, which harbors the ectosymbionts, a microbial community has also been reported in the cephalothorax of R. chacei. Previous data on morphology, behavior and isotopic values indicate a diet based on a combination of feeding...
Tipo: Text
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00465/57680/59880.pdf

Microbial diversity associated with the hydrothermal shrimp Rimicaris exoculata gut and occurrence of a resident microbial community
Provedor de dados: 5
Autores: Durand, Lucile; Zbinden, Magali; Cueff-gauchard, Valerie; Duperron, Sebastien; Roussel, Erwan; Shillito, Bruce; Cambon-bonavita, Marie-anne.
Rimicaris exoculata dominates the megafauna of several Mid-Atlantic Ridge hydrothermal sites. Its gut is full of sulphides and iron-oxide particles and harbours microbial communities. Although a trophic symbiosis has been suggested, their role remains unclear. In vivo starvation experiments in pressurized vessels were performed on shrimps from Rainbow and Trans-Atlantic Geotraverse sites in order to expel the transient gut contents. Microbial communities associated with the gut of starved and reference shrimps were compared using 16S rRNA gene libraries and microscopic observations (light, transmission and scanning electron microscopy and FISH analyses). We show that the gut microbiota of shrimps from both sites included mainly Deferribacteres, Mollicutes,...
Tipo: Text
Palavras-chave: Deferribacteres; Midgut epibiosis; Mollicutes; Proteobacteria; Rimicaris exoculata; Starvation experiment.
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00000/11142/7919.pdf

Is It First the Egg or the Shrimp? – Diversity and Variation in Microbial Communities Colonizing Broods of the Vent Shrimp Rimicaris exoculata During Embryonic Development
Provedor de dados: 5
Autores: Methou, Pierre; Hernández-ávila, Ivan; Aube, Johanne; Cueff-gauchard, Valerie; Gayet, Nicolas; Amand, Louis; Shillito, Bruce; Pradillon, Florence; Cambon-bonavita, Marie-anne.
Rimicaris exoculata is one of the most well-known and emblematic species of endemic vent fauna. Like many other species from these ecosystems, Rimicaris shrimps host important communities of chemosynthetic bacteria living in symbiosis with their host inside the cephalothorax and gut. For many of these symbiotic partners, the mode of transmission remains to be elucidated and the starting point of the symbiotic relationship is not yet defined, but could begin with the egg. In this study, we explored the proliferation of microbial communities on R. exoculata broods through embryonic development using a combination of NGS sequencing and microscopy approaches. Variations in abundance and diversity of egg microbial communities were analyzed in broods at...
Tipo: Text
Palavras-chave: Hydrothermal; Shrimp; Microbial colonization; Alvinocarididae; Egg development.
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00489/60067/63352.pdf

Highlighting of quorum sensing lux genes and their expression in the hydrothermal vent shrimp Rimicaris exoculata ectosymbiontic community. Possible use as biogeographic markers
Provedor de dados: 5
Autores: Le Bloa, Simon; Durand, Lucile; Cueff-gauchard, Valerie; Le Bars, Josiane; Taupin, Laure; Marteau, Charlotte; Bazire, Alexis; Cambon-bonavita, Marie-anne.
Rimicaris exoculata is a caridean shrimp that dominates the fauna at several hydrothermal vent sites of the Mid-Atlantic Ridge. It has two distinct and stable microbial communities. One of these epibiontic bacterial communities is located in the shrimp gut and has a distribution and role that are poorly understood. The second colonizes its enlarged gill chamber and is involved in host nutrition. It is eliminated after each molt, and has colonization processes reminiscent of those of a biofilm. The presence and expression of genes usually involved in quorum sensing (QS) were then studied. At four sites, Rainbow, TAG, Snake Pit and Logatchev, two lux genes were identified in the R. exoculata epibiontic community at different shrimp molt stages and life...
Tipo: Text
Ano: 2017
URL: http://archimer.ifremer.fr/doc/00377/48841/49268.pdf

Detailed investigation of hydrothermal site Rainbow, Mid-Atlantic Ridge, 36°13’N: Cruise MoMARDream
Provedor de dados: 5
Autores: Dyment, J.; Bissessur, D.; Bucas, Karenn; Cueff-gauchard, Valerie; Durand, Lucile; Fouquet, Yves; Gaill, F.; Gente, P.; Hoise, E.; Ildefonse, B.; Konn, Cecile; Lartaud, F.; Le Bris, Nadine; Musset, G.; Nunes, A.; Renard, J.; Riou, V.; Tasiemski, A.; Thibaud, R.; Torres, P.; Yatheesh, V.; Vodjdani, I.; Zbinden, M.
Tipo: Text
Ano: 2009
URL: http://archimer.ifremer.fr/doc/00385/49651/50164.pdf

Evaluating sediment and water sampling methods for the estimation of deep-sea biodiversity using environmental DNA
Provedor de dados: 5
Autores: Brandt, Miriam; Pradillon, Florence; Trouche, Blandine; Henry, Nicolas; Liautard-haag, Cathy; Cambon-bonavita, Marie-anne; Cueff-gauchard, Valerie; Wincker, Patrick; Belser, Caroline; Poulain, Julie; Arnaud-haond, Sophie; Zeppilli, Daniela.
Despite representing one of the largest biomes on earth, biodiversity of the deep seafloor is still poorly known. Environmental DNA metabarcoding offers prospects for fast inventories and surveys, yet requires standardized sampling approaches and careful choice of environmental substrate. Here, we aimed to optimize the genetic assessment of prokaryote (16S), protistan (18S V4), and metazoan (18S V1–V2, COI) communities, by evaluating sampling strategies for sediment and aboveground water, deployed simultaneously at one deep-sea site. For sediment, while size-class sorting through sieving had no significant effect on total detected alpha diversity and resolved similar taxonomic compositions at the phylum level for all markers studied, it effectively...
Tipo: Text
Palavras-chave: Biodiversity; Ecological genetics; Microbial ecology; Molecular ecology.
Ano: 2021
URL: https://archimer.ifremer.fr/doc/00689/80094/83149.pdf

Biogeographical distribution of Rimicaris exoculata resident gut epibiont communities along the Mid-Atlantic Ridge hydrothermal vent sites
Provedor de dados: 5
Autores: Durand, Lucile; Roumagnac, Marie; Cueff-gauchard, Valerie; Jan, Cyrielle; Guri, Mathieu; Tessier, Claire; Haond, Marine; Crassous, Philippe; Zbinden, Magali; Arnaud-haond, Sophie; Cambon-bonavita, Marie-anne.
Rimicaris exoculata is a deep-sea hydrothermal vent shrimp which enlarged gill chamber houses a complex trophic epibiotic community. Its gut harbours an autochthonous and distinct microbial community. This species dominates hydrothermal ecosystems megafauna along the Mid-Atlantic Ridge, regardless of contrasted geochemical conditions prevailing in them. Here, the resident gut epibiont community at four contrasted hydrothermal vent sites (Rainbow/TAG/Logatchev/Ashadze) was analysed and compiled with previous data to evaluate the possible influence of site location, using 16S rRNA surveys and microscopic observations (TEM, SEM and FISH analyses). Filamentous epibionts inserted between the epithelial cells microvilli were observed on all examined samples....
Tipo: Text
Palavras-chave: Epibiont; Extreme environments; Network analyses; Phylogeography.
Ano: 2015
URL: http://archimer.ifremer.fr/doc/00288/39890/38443.pdf

Development of an ecotoxicological protocol for the deep-sea fauna using the hydrothermal vent shrimp Rimicaris exoculata
Provedor de dados: 5
Autores: Auguste, M.; Mestre, N. C.; Rocha, T. L.; Cardoso, C.; Cueff-gauchard, Valerie; Le Bloa, Simon; Cambon-bonavita, Marie-anne; Shillito, B.; Zbinden, M.; Ravaux, J.; Bebianno, M. J.
In light of deep-sea mining industry development, particularly interested in massive-sulphide deposits enriched in metals with high commercial value, efforts are increasing to better understand potential environmental impacts to local fauna. The aim of this study was to assess the natural background levels of biomarkers in the hydrothermal vent shrimp Rimicaris exoculata and their responses to copper exposure at in situ pressure (30 MPa) as well as the effects of depressurization and pressurization of the high-pressure aquarium IPOCAMP. R. exoculata were collected from the chimney walls of the hydrothermal vent site TAG (Mid Atlantic Ridge) at 3630 m depth during the BICOSE cruise in 2014. Tissue metal accumulation was quantified in different tissues...
Tipo: Text
Palavras-chave: Deep-sea mining; Hydrothermal vents; Biomarkers; Ipocamp; Copper; TAG.
Ano: 2016
URL: http://archimer.ifremer.fr/doc/00323/43465/42869.pdf

Acquisition of epibiotic bacteria along the life cycle of the hydrothermal shrimp Rimicaris exoculata
Provedor de dados: 5
Autores: Guri, Mathieu; Durand, Lucile; Cueff-gauchard, Valerie; Zbinden, Magali; Crassous, Philippe; Shillito, Bruce; Cambon-bonavita, Marie-anne.
The caridean shrimp Rimicaris exoculata dominates the fauna at several Mid-Atlantic Ridge hydrothermal vent sites. This shrimp has an enlarged gill chamber, harboring a dense ectosymbiotic community of chemoautotrophic bacteria associated with mineral oxide deposits. Until now, their acquisition is not fully understood. At three hydrothermal vent sites, we analyzed the epibionts diversity at different moult stages and also in the first stages of the shrimp life (eggs, hatched eggs (with larvae) and juveniles). Hatched eggs associated with young larvae were collected for the first time directly from gravid females at the Logachev vent site during the Serpentine cruise. An approach using 16S rRNA clone libraries, scanning and transmission electron...
Tipo: Text
Palavras-chave: Symbiosis; Larvae; Methanotrophic symbiont; Rimicaris exoculata; Transmission pathways.
Ano: 2012
URL: http://archimer.ifremer.fr/doc/00072/18318/15997.pdf

Optimisation of growth conditions for continuous culture of the hyperthermophilic archaeon Thermococcus hydrothermalis and development of sulphur-free defined and minimal media
Provedor de dados: 5
Autores: Postec, Anne; Pignet, Patricia; Cueff-gauchard, Valerie; Schmitt, Anne; Querellou, Joel; Godfroy, Anne.
The hyperthermophilic archaeon Thermococcus hydrothermalis was cultivated in continuous culture in a gas-lift bioreactor in the absence of elemental sulphur on both proteinaceous and maltose-containing media. Optimal conditions (pH, temperature and gas flow rate), determined on complex media that yielded maximal growth rate and maximal steady state cell density, were obtained at 80degreesC, pH 6 and gas sparging at 0.2 v v(-1) min(-1). Higher steady state cell densities were obtained on a medium containing maltose and yeast extract. In order to design a defined and minimal media, the nutritional requirements of T hydrothermalis were then investigated using continuous culture in the absence of elemental sulphur in the gas-lift bioreactor. First, the complex...
Tipo: Text
Palavras-chave: Bioreactor; Defined and minimal media; Culture optimisation; Gas lift bioreactor; Thermococcus hydrothermalis.
Ano: 2005
URL: http://archimer.ifremer.fr/doc/2005/publication-365.pdf

Thermosipho atlanticus sp nov., a novel member of the Thermotogales isolated from a Mid-Atlantic Ridge hydrothermal vent
Provedor de dados: 5
Autores: Urios, Laurent; Cueff-gauchard, Valerie; Pignet, Patricia; Postec, Anne; Fardeau, Marie-laure; Ollivier, Bernard; Barbier, Georges.
A novel anaerobic, thermophilic and heterotrophic bacterium, designated strain DV114O(T), was isolated from a deep-sea hydrothermal vent sample from the Mid-Atlantic Ridge. The cells were non-motile straight rods, 1-8 mum long and 0-4 mum wide, surrounded by an outer sheath-like structure (toga). They grew at 45-80 degreesC (optimum 65 degreesC), pH 5-0-9-0 (optimum pH 6-0) and at sea salt concentrations of 20-60 gl(-1) (optimum 30 gl(-1)). Strain DV1140(T) was able to ferment yeast extract, peptone, brain heart infusion, gelatin, starch, galactose, arabinose, glucose, trehalose and cellobiose. The fermentation products identified on glucose in the presence of yeast extract and peptone were acetate, isovalerate and hydrogen. The G + C content of the...
Tipo: Text
Palavras-chave: Marine microorganisms; Thermosipho atlanticus; Thermotogales; Thermophile; Deep sea vent.
Ano: 2004
URL: http://archimer.ifremer.fr/doc/2004/publication-362.pdf

Antimicrobial Peptides and Ectosymbiotic Relationships: Involvement of a Novel Type IIa Crustin in the Life Cycle of a Deep-Sea Vent Shrimp
Provedor de dados: 5
Autores: Le Bloa, Simon; Boidin-wichlacz, Céline; Cueff-gauchard, Valerie; Rosa, Rafael Diego; Cuvillier-hot, Virginie; Durand, Lucile; Methou, Pierre; Pradillon, Florence; Cambon-bonavita, Marie-anne; Tasiemski, Aurélie.
The symbiotic shrimp Rimicaris exoculata dominates the macrofauna inhabiting the active smokers of the deep-sea mid Atlantic ridge vent fields. We investigated the nature of the host mechanisms controlling the vital and highly specialized ectosymbiotic community confined into its cephalothoracic cavity. R. exoculata belongs to the Pleocyemata, crustacean brooding eggs, usually producing Type I crustins. Unexpectedly, a novel anti-Gram-positive type II crustin was molecularly identified in R. exoculata. Re-crustin is mainly produced by the appendages and the inner surfaces of the cephalothoracic cavity, embedding target epibionts. Symbiosis acquisition and regulating mechanisms are still poorly understood. Yet, symbiotic communities were identified at...
Tipo: Text
Palavras-chave: Extreme; Hydrothermal; Symbiosis; Host-microbe interaction; Invertebrate immunity; Crustacean.
Ano: 2020
URL: https://archimer.ifremer.fr/doc/00643/75534/76413.pdf

Draft Genome Sequences of Two  Marinitoga camini  Isolates Producing Bacterioviruses
Provedor de dados: 5
Autores: Mercier, Coraline; Lossouarn, Julien; Haverkamp, Thomas; Bienvenu, Nadège; Godfroy, Anne; Cueff-gauchard, Valerie; Geslin, Claire; Nesbo, Camilla.
Here, we present the draft genome sequences of two thermophilic Marinitoga strain members of the Thermotogales order, Marinitoga camini DV1155 and Marinitoga camini DV1197. These strains were isolated from deep-sea hydrothermal vents of the Mid-Atlantic Ridge.
Tipo: Text
Ano: 2016
URL: https://archimer.ifremer.fr/doc/00357/46833/46708.pdf

A Nematode of the Mid-Atlantic Ridge Hydrothermal Vents Harbors a Possible Symbiotic Relationship
Provedor de dados: 5
Autores: Bellec, Laure; Cambon-bonavita, Marie-anne; Cueff-gauchard, Valerie; Durand, Lucile; Gayet, Nicolas; Zeppilli, Daniela.
Deep-sea hydrothermal vent meiofauna have been the focus of recent research and the discovery of an abundant well-adapted free-living marine nematode on the Mid-Atlantic Ridge offers new perspectives on adaptations to the vent environment. Indeed, knowledge concerning biological interactions of microbes and meiofauna in marine extreme environments is scarce, especially for nematodes. In this study, we used microscopic observations [fluorescence in situ hybridization (FISH) and scanning electron microscopy (SEM)] and metabarcoding of 16S rRNA to characterize the bacterial community of the nematode species Oncholaimus dyvae, an overlooked but ecologically important vent organism. Detection of bacteria in the buccal cavity and on the cuticle (SEM) and...
Tipo: Text
Palavras-chave: Sulfur-oxidizing bacteria; Lucky Strike vent field; Meiofauna; Nematode; Endosymbiont.
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00457/56846/58676.pdf

Niche partitioning in the Rimicaris exoculata holobiont: the case of the first symbiotic Zetaproteobacteria
Provedor de dados: 5
Autores: Cambon-bonavita, Marie-anne; Aubé, Johanne; Cueff-gauchard, Valerie; Reveillaud, Julie.
Background Free-living and symbiotic chemosynthetic microbial communities support primary production and higher trophic levels in deep-sea hydrothermal vents. The shrimp Rimicaris exoculata, which dominates animal communities along the Mid-Atlantic Ridge, houses a complex bacterial community in its enlarged cephalothorax. The dominant bacteria present are from the taxonomic groups Campylobacteria, Desulfobulbia (formerly Deltaproteobacteria), Alphaproteobacteria, Gammaproteobacteria, and some recently discovered iron oxyhydroxide-coated Zetaproteobacteria. This epibiotic consortium uses iron, sulfide, methane, and hydrogen as energy sources. Here, we generated shotgun metagenomes from Rimicaris exoculata cephalothoracic epibiotic communities to reconstruct...
Tipo: Text
Palavras-chave: Niche partitioning; Rimicaris; Metagenome-assembled genomes; Zetaproteobacteria; Holobiont; Hydrothermal vents.
Ano: 2021
URL: https://archimer.ifremer.fr/doc/00689/80089/83128.pdf