Consulta: creatorFacets:"Didoné,Dielli Aparecida"
Registros recuperados: 2
Data/hora: 10/06/2024 04:37:44

Adaptation of in vitro regeneration protocol for Brazilian wheat genotypes
Provedor de dados: 65
Autores: Roesler,Eduardo André; Manfroi,Ernandes; Morás,Andréa; Didoné,Dielli Aparecida; Grando,Magali Ferrari; Lau,Elene Yamazaki.
ABSTRACT:  The availability of an efficient protocol for in vitro regeneration is imperative for genetic transformation. Using genotypes adapted to the target region as a transgenic platform accelerates the development of cultivars. Therefore, this study aimed to adapt an in vitro regeneration protocol for Brazilian wheat genotypes. For this purpose, the in vitro regeneration capacity of immature embryos from six Brazilian wheat genotypes using two protocols of regeneration of somatic embryos was analysed. Furthermore, combinations of 2,4-D and picloram in the callus induction medium were tested in order to improve regeneration efficiency. Genotypes with higher regeneration efficiency were BR18-Terena and PF020037, yielding 0.42 and 1.13 shoots per explant...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Triticum aestivum; Tissue culture; Growth regulators.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018001100402

Genetic transformation of the Brazilian BR 451 maize variety by the Agrobacterium tumefaciens method
Provedor de dados: 65
Autores: Silva,Marilia Rodrigues de; Didoné,Dielli Aparecida; Ceccon,Cássia Canzi; Almeida,Vinícius de Oliveira; Grando,Magali Ferrari.
ABSTRACT: The asexually gene introduction by genetic engineering has brought enormous possibilities to innovate plant breeding. However, principally because of the low in vitro response, genetic transformation has been restricted to only certain genotypes of agronomically significant species. With the objective of establishing a protocol for genetically transforming the Brazilian BR 451 maize variety through Agrobacterium tumefaciens, it was studied the capacity of plant regeneration in vitro from embryogenic calli cultivated in three regeneration media, each having different growth regulators. It was also evaluated the temperature stress effect on the transformation of the immature embryos with A. tumefaciens EHA 101 containing the plasmid pTF102 with...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: In vitro regeneration; Temperature stress; EHA 101; PTF102; Zea mays.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017001100151