Consulta: creatorFacets:"Dufy, Christian"
Registros recuperados: 3
Data/hora: 11/06/2024 10:12:15

Polyploidy in the Manila clam Ruditapes philippinarum. II. Chemical induction of tetraploid embryos
Provedor de dados: 5
Autores: Diter, Alain; Dufy, Christian.
Tetraploidy was induced in embryos of Ruditapes philippinarum by treating the eggs with cytochalasin B. (CB). Two treatment times were found to be effective in inducing tetraplidy : 0 to 10 minutes after insemination (before formation of the first polar body) and 45 minutes after insemination (just before the first cleavage). Treatments, started 5 and 45 minutes after insemination, produced 64.4 ± 7.8% and 28.3 ± 2.3% tetraploid embrios, respectively, after 6 hours (on the average over 3 experiments). However, no tetraploids were detected in 4-month-old spat. Sperm entry delayed by the CB could be involved in induction of tetraploidy by early treatment.
Tipo: Text
Palavras-chave: Ruditapes philippinarum; Bivalve; Tetraploidy; Cytochalasin B.; Ruditapes philippinarum; Bivalve; Tétraploïdie; Cytochalasine B.
Ano: 1990
URL: http://archimer.ifremer.fr/doc/1990/publication-2753.pdf

Analyse du circuit d'alimentation en eau de mer de l'écloserie du L.P.G.I.M. Ronce les Bains
Provedor de dados: 5
Autores: Dufy, Christian.
Cette étude préliminaire a été réalisée au cours de 7 semaines et se base sur 21 relevés successifs (du 15 Juillet au 11 Août 1987) . Elle visait essentiellement à mettre en place une méthodologie d'analyse la plus directe possible et propre à visualiser ultérieurement, par rapport aux données premières, une évolution des différents paramètres étudiés . Ceci, tant en fonction du vieillissement des circuits que des fluctuations des paramètres estuariens ou bien encore selon les modifications susceptibles d'être apportées aux installations. Par conséquent, ce rapport est construit de manière à permettre la réalisat ion de nouvelles expériences dans des conditons similaires. Dans un but évolutif,  il sera donc possibl e de reprendre les données et d' y avoir...
Tipo: Text
Ano: 1987
URL: http://archimer.ifremer.fr/doc/00426/53733/54608.pdf

Polyploidy in the Manila clam Ruditapes philippinarum - 1 - Chemical induction and larval performances of triploids
Provedor de dados: 5
Autores: Dufy, Christian; Diter, Alain.
An effective treatment using catochalasin B (1 mg CB/ml dimethylsulfoxyde in 1 litre of sea water) was developed to induce triploidy in the Manila clam Ruditapes philippinurum (Adams and Reeve). The percentage of induced triploidy was assessed by karyological examination of embryos and spat. The treatment was found to be optimal between 20 to 35 minutes post-insemination exposure to CB, at 25'C, and produced 75.8 ± 5.7% triploid embryos on the average over four experiments. Growth and survival of larvae to metamorphosis were recorded for two different rearings each split into two replicates. Both larval rearings showed similar survival up to settlement: 19.3 and 27.0%, on average over two replicates, in the treated batches relative to the controls. Mean...
Tipo: Text
Palavras-chave: Larval survival; Larval growth; Bivalves; Cytochalasin B; Triploidy; Sruvie larvaire; Croissance larvaire; Bivalve; Cytochalasine B; Triploïdie.
Ano: 1990
URL: http://archimer.ifremer.fr/doc/1990/publication-2752.pdf