Consulta: creatorFacets:"Durand, Lucile"
Registros recuperados: 11
Data/hora: 06/06/2024 14:14:08

Investigation of bacterial communities within the digestive organs of the hydrothermal vent shrimp Rimicaris exoculata provide insights into holobiont geographic clustering
Provedor de dados: 5
Autores: Cowart, Dominique; Durand, Lucile; Cambon-bonavita, Marie-anne; Arnaud-haond, Sophie.
Prokaryotic communities forming symbiotic relationships with the vent shrimp, Rimicaris exoculata, are well studied components of hydrothermal ecosystems at the Mid-Atlantic Ridge (MAR). Despite the tight link between host and symbiont, the observed lack of spatial genetic structure seen in R. exoculata contrasts with the geographic differentiation detected in specific bacterial ectosymbionts. The geographic clustering of bacterial lineages within a seemingly panmictic host suggests either the presence of finer scale restriction to gene flow not yet detected in the host, horizontal transmission (environmental selection) of its endosymbionts as a consequence of unique vent geochemistry, or vertically transmitted endosymbionts that exhibit genetic...
Tipo: Text
Ano: 2017
URL: https://archimer.ifremer.fr/doc/00376/48705/49058.pdf

Microbial diversity associated with the hydrothermal shrimp Rimicaris exoculata gut and occurrence of a resident microbial community
Provedor de dados: 5
Autores: Durand, Lucile; Zbinden, Magali; Cueff-gauchard, Valerie; Duperron, Sebastien; Roussel, Erwan; Shillito, Bruce; Cambon-bonavita, Marie-anne.
Rimicaris exoculata dominates the megafauna of several Mid-Atlantic Ridge hydrothermal sites. Its gut is full of sulphides and iron-oxide particles and harbours microbial communities. Although a trophic symbiosis has been suggested, their role remains unclear. In vivo starvation experiments in pressurized vessels were performed on shrimps from Rainbow and Trans-Atlantic Geotraverse sites in order to expel the transient gut contents. Microbial communities associated with the gut of starved and reference shrimps were compared using 16S rRNA gene libraries and microscopic observations (light, transmission and scanning electron microscopy and FISH analyses). We show that the gut microbiota of shrimps from both sites included mainly Deferribacteres, Mollicutes,...
Tipo: Text
Palavras-chave: Deferribacteres; Midgut epibiosis; Mollicutes; Proteobacteria; Rimicaris exoculata; Starvation experiment.
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00000/11142/7919.pdf

Highlighting of quorum sensing lux genes and their expression in the hydrothermal vent shrimp Rimicaris exoculata ectosymbiontic community. Possible use as biogeographic markers
Provedor de dados: 5
Autores: Le Bloa, Simon; Durand, Lucile; Cueff-gauchard, Valerie; Le Bars, Josiane; Taupin, Laure; Marteau, Charlotte; Bazire, Alexis; Cambon-bonavita, Marie-anne.
Rimicaris exoculata is a caridean shrimp that dominates the fauna at several hydrothermal vent sites of the Mid-Atlantic Ridge. It has two distinct and stable microbial communities. One of these epibiontic bacterial communities is located in the shrimp gut and has a distribution and role that are poorly understood. The second colonizes its enlarged gill chamber and is involved in host nutrition. It is eliminated after each molt, and has colonization processes reminiscent of those of a biofilm. The presence and expression of genes usually involved in quorum sensing (QS) were then studied. At four sites, Rainbow, TAG, Snake Pit and Logatchev, two lux genes were identified in the R. exoculata epibiontic community at different shrimp molt stages and life...
Tipo: Text
Ano: 2017
URL: http://archimer.ifremer.fr/doc/00377/48841/49268.pdf

Marine culturable yeasts in deep-sea hydrothermal vents: species richness and association with fauna
Provedor de dados: 5
Autores: Burgaud, Gaetan; Arzur, Danielle; Durand, Lucile; Cambon-bonavita, Marie-anne; Barbier, Georges.
Investigations of the diversity of culturable yeasts at deep-sea hydrothermal sites have suggested possible interactions with endemic fauna. Samples were collected during various oceanographic cruises at the Mid-Atlantic Ridge, South Pacific Basins and East Pacific Rise. Cultures of 32 isolates, mostly associated with animals, were collected. Phylogenetic analyses of 26S rRNA gene sequences revealed that the yeasts belonged to Ascomycota and Basidiomycota phyla, with the identification of several genera: Rhodotorula, Rhodosporidium, Candida, Debaryomyces and Cryptococcus. Those genera are usually isolated from deep-sea environments. To our knowledge, this is the first report of yeasts associated with deep-sea hydrothermal animals.
Tipo: Text
Palavras-chave: Yeasts; Hydrothermal vents; Fauna; 26S rRNA gene; FISH.
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00006/11704/8475.pdf

Detailed investigation of hydrothermal site Rainbow, Mid-Atlantic Ridge, 36°13’N: Cruise MoMARDream
Provedor de dados: 5
Autores: Dyment, J.; Bissessur, D.; Bucas, Karenn; Cueff-gauchard, Valerie; Durand, Lucile; Fouquet, Yves; Gaill, F.; Gente, P.; Hoise, E.; Ildefonse, B.; Konn, Cecile; Lartaud, F.; Le Bris, Nadine; Musset, G.; Nunes, A.; Renard, J.; Riou, V.; Tasiemski, A.; Thibaud, R.; Torres, P.; Yatheesh, V.; Vodjdani, I.; Zbinden, M.
Tipo: Text
Ano: 2009
URL: http://archimer.ifremer.fr/doc/00385/49651/50164.pdf

Biogeographical distribution of Rimicaris exoculata resident gut epibiont communities along the Mid-Atlantic Ridge hydrothermal vent sites
Provedor de dados: 5
Autores: Durand, Lucile; Roumagnac, Marie; Cueff-gauchard, Valerie; Jan, Cyrielle; Guri, Mathieu; Tessier, Claire; Haond, Marine; Crassous, Philippe; Zbinden, Magali; Arnaud-haond, Sophie; Cambon-bonavita, Marie-anne.
Rimicaris exoculata is a deep-sea hydrothermal vent shrimp which enlarged gill chamber houses a complex trophic epibiotic community. Its gut harbours an autochthonous and distinct microbial community. This species dominates hydrothermal ecosystems megafauna along the Mid-Atlantic Ridge, regardless of contrasted geochemical conditions prevailing in them. Here, the resident gut epibiont community at four contrasted hydrothermal vent sites (Rainbow/TAG/Logatchev/Ashadze) was analysed and compiled with previous data to evaluate the possible influence of site location, using 16S rRNA surveys and microscopic observations (TEM, SEM and FISH analyses). Filamentous epibionts inserted between the epithelial cells microvilli were observed on all examined samples....
Tipo: Text
Palavras-chave: Epibiont; Extreme environments; Network analyses; Phylogeography.
Ano: 2015
URL: http://archimer.ifremer.fr/doc/00288/39890/38443.pdf

Acquisition of epibiotic bacteria along the life cycle of the hydrothermal shrimp Rimicaris exoculata
Provedor de dados: 5
Autores: Guri, Mathieu; Durand, Lucile; Cueff-gauchard, Valerie; Zbinden, Magali; Crassous, Philippe; Shillito, Bruce; Cambon-bonavita, Marie-anne.
The caridean shrimp Rimicaris exoculata dominates the fauna at several Mid-Atlantic Ridge hydrothermal vent sites. This shrimp has an enlarged gill chamber, harboring a dense ectosymbiotic community of chemoautotrophic bacteria associated with mineral oxide deposits. Until now, their acquisition is not fully understood. At three hydrothermal vent sites, we analyzed the epibionts diversity at different moult stages and also in the first stages of the shrimp life (eggs, hatched eggs (with larvae) and juveniles). Hatched eggs associated with young larvae were collected for the first time directly from gravid females at the Logachev vent site during the Serpentine cruise. An approach using 16S rRNA clone libraries, scanning and transmission electron...
Tipo: Text
Palavras-chave: Symbiosis; Larvae; Methanotrophic symbiont; Rimicaris exoculata; Transmission pathways.
Ano: 2012
URL: http://archimer.ifremer.fr/doc/00072/18318/15997.pdf

Microbial Communities of the Shallow-Water Hydrothermal Vent Near Naples, Italy, and Chemosynthetic Symbionts Associated With a Free-Living Marine Nematode
Provedor de dados: 5
Autores: Bellec, Laure; Cambon-bonavita, Marie-anne; Durand, Lucile; Aube, Johanne; Gayet, Nicolas; Sandulli, Roberto; Brandily, Christophe; Zeppilli, Daniela.
Shallow-water hydrothermal vents are widespread, especially in the Mediterranean Sea, owing to the active volcanism of the area. Apart free microbial communities’ investigations, few biological studies have been leaded yet. Investigations of microbial communities associated with Nematoda, an ecologically important group in sediments, can help to improve our overall understanding of these ecosystems. We used a multidisciplinary-approach, based on microscopic observations (scanning electron microscopy: SEM and Fluorescence In Situ Hybridization: FISH) coupled with a molecular diversity analysis using metabarcoding, based on the 16S rRNA gene (V3-V4 region), to characterize the bacterial community of a free-living marine nematode and its environment, the...
Tipo: Text
Palavras-chave: Nematode; Shallow-water hydrothermal vent; Sulfur-oxidizing bacteria; Iron cycle; Zetaproteobacteria.
Ano: 2020
URL: https://archimer.ifremer.fr/doc/00643/75544/76449.pdf

Antimicrobial Peptides and Ectosymbiotic Relationships: Involvement of a Novel Type IIa Crustin in the Life Cycle of a Deep-Sea Vent Shrimp
Provedor de dados: 5
Autores: Le Bloa, Simon; Boidin-wichlacz, Céline; Cueff-gauchard, Valerie; Rosa, Rafael Diego; Cuvillier-hot, Virginie; Durand, Lucile; Methou, Pierre; Pradillon, Florence; Cambon-bonavita, Marie-anne; Tasiemski, Aurélie.
The symbiotic shrimp Rimicaris exoculata dominates the macrofauna inhabiting the active smokers of the deep-sea mid Atlantic ridge vent fields. We investigated the nature of the host mechanisms controlling the vital and highly specialized ectosymbiotic community confined into its cephalothoracic cavity. R. exoculata belongs to the Pleocyemata, crustacean brooding eggs, usually producing Type I crustins. Unexpectedly, a novel anti-Gram-positive type II crustin was molecularly identified in R. exoculata. Re-crustin is mainly produced by the appendages and the inner surfaces of the cephalothoracic cavity, embedding target epibionts. Symbiosis acquisition and regulating mechanisms are still poorly understood. Yet, symbiotic communities were identified at...
Tipo: Text
Palavras-chave: Extreme; Hydrothermal; Symbiosis; Host-microbe interaction; Invertebrate immunity; Crustacean.
Ano: 2020
URL: https://archimer.ifremer.fr/doc/00643/75534/76413.pdf

A Nematode of the Mid-Atlantic Ridge Hydrothermal Vents Harbors a Possible Symbiotic Relationship
Provedor de dados: 5
Autores: Bellec, Laure; Cambon-bonavita, Marie-anne; Cueff-gauchard, Valerie; Durand, Lucile; Gayet, Nicolas; Zeppilli, Daniela.
Deep-sea hydrothermal vent meiofauna have been the focus of recent research and the discovery of an abundant well-adapted free-living marine nematode on the Mid-Atlantic Ridge offers new perspectives on adaptations to the vent environment. Indeed, knowledge concerning biological interactions of microbes and meiofauna in marine extreme environments is scarce, especially for nematodes. In this study, we used microscopic observations [fluorescence in situ hybridization (FISH) and scanning electron microscopy (SEM)] and metabarcoding of 16S rRNA to characterize the bacterial community of the nematode species Oncholaimus dyvae, an overlooked but ecologically important vent organism. Detection of bacteria in the buccal cavity and on the cuticle (SEM) and...
Tipo: Text
Palavras-chave: Sulfur-oxidizing bacteria; Lucky Strike vent field; Meiofauna; Nematode; Endosymbiont.
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00457/56846/58676.pdf

Chemosynthetic ectosymbionts associated with a shallow-water marine nematode
Provedor de dados: 5
Autores: Bellec, Laure; Cambon Bonavita, Marie-anne; Hourdez, Stéphane; Jebbar, Mohamed; Tasiemski, Aurélie; Durand, Lucile; Gayet, Nicolas; Zeppilli, Daniela.
Prokaryotes and free-living nematodes are both very abundant and co-occur in marine environments, but little is known about their possible association. Our objective was to characterize the microbiome of a neglected but ecologically important group of free-living benthic nematodes of the Oncholaimidae family. We used a multi-approach study based on microscopic observations (Scanning Electron Microscopy and Fluorescence In Situ Hybridization) coupled with an assessment of molecular diversity using metabarcoding based on the 16S rRNA gene. All investigated free-living marine nematode specimens harboured distinct microbial communities (from the surrounding water and sediment and through the seasons) with ectosymbiosis seemed more abundant during summer....
Tipo: Text
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00494/60614/64098.pdf