Consulta: creatorFacets:"Elston, Ralph"
Registros recuperados: 2
Data/hora: 10/06/2024 15:23:29

Serological and analysis of Bonamia in Ostrea edulis and Tiostrea lutaria using polyclonal and monoclonal antibodies
Provedor de dados: 5
Autores: Mialhe, Eric; Boulo, Viviane; Elston, Ralph; Hill, Barry; Hine, Michel; Montes, Jaime; Mont, Jaime; Banning, Paul Van; Grizel, Henri.
Murine moncolonal and polyclonal antibodies to antigesn of Bonamia ostreae were assessed for serological comparaison of parasites associated with different geographical originns of flat oyster, Ostrea edulis, and with Tiostrea lutaria. All Bonamia isolates from O. edulis reacted similary with polyclonal and monoclonal antibodies. The parasite associed with Tiostrea lutaria which lacks reactivity with 20 B 2 MAB must be considered as a serologically distinct from of B. ostreae
Tipo: Text
Palavras-chave: Serological comparison; Polyclonal and monoclonal antibodies; Tiostrea lutaria; Ostrea edulis; Bonamia; Comparaison sérologique; Anticorps polyclonaux et monoclonaux; Tiostrea lutaria; Ostrea edulis; Bonamia.
Ano: 1988
URL: http://archimer.ifremer.fr/doc/1988/publication-3101.pdf

Preparation and characterization of monoclonal antibodies against neoplastic hemocytes of Mytilus edulis (Bivalvia)
Provedor de dados: 5
Autores: Noel, Danielle; Boulo, Viviane; Chagot, Dominique; Mialhe, Eric; Paolucci, Francis; Clavies, Christine; Hervaud, Eric; Elston, Ralph.
Mytilus edulis develop progressive and fatal hemocytic neoplasia. We have generated monoclonal antibodies using neoplastic hemocytes from highly infected individuals collected from Puget Sound in Washington State (USA). Twenty-seven monoclonal antibodies against neoplatic cell antigens were obtained. Specificity of 2 purified antibodies, 14F1 and 16G10, was demonstrated by immunofluorescence when  incubated with neoplastic hemocytes, whereas these anbidodies did not react with normal cells. This specificity was confirmed by immunogold labelling and electron microscopic visualization. The technique permitted localization of the epitopes recognized by the monoclonal antibodies on the plasma membrane. These monoclonal antibodies are suitable reagents for...
Tipo: Text
Ano: 1991
URL: http://archimer.ifremer.fr/doc/1991/publication-2757.pdf