Consulta: creatorFacets:"Fóscolo,Mabel"
Registros recuperados: 4
Data/hora: 02/06/2024 04:52:47

Seasonal variations in the heterologous binding of viscacha spermatozoa: A scanning electron microscope study
Provedor de dados: 47
Autores: Aguilera Merlo,Claudia; Muñoz,Estela; Dominguez,Susana; Fóscolo,Mabel; Scardapane,Luis; de Rosas,Juan Carlos.
Seasonal changes in the reproductive activity of the adult male viscacha (Lagostomus maximus maximus) were investigated during the annual reproductive cycle. Assays of heterologous in vitro binding between compatible gametes were used to evaluate the ability of viscacha spermatozoa to achieve primary binding during its annual reproductive cycle. Sperm were collected by mincing cauda epididymis in HECM-3 medium and the sperm concentration and motility were evaluated. Cumulus-free and zona-free oocytes obtained from superovulated hamsters were inseminated in vitro with capacitated sperm suspensions, incubated at 37ºC, 5% CO2 for 3 h, and then processed for studies by scanning electronic microscopy. Statistical analysis was used to compare the quantitative...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Viscacha (Lagostomus maximus maximus); Spermatozoa; Heterologous binding; Seasonal variations.
Ano: 2005
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452005000300001

Single-channel response of hamster oocytes to ertilization with homologous spermatozoa
Provedor de dados: 47
Autores: Ituarte,Leonor M.E.; Viera,Teresa B.; Saldeña,Teobaldo A.; De Rosas,Juan C.; Fóscolo,Mabel; Ibáñez,Jorge E.; Saraví,Fernando D.
Electrophysiological events occur early after fertilization, along with changes in intracellular Ca2+ concentration . Passive electrical parameters were determined in golden hamster oocytes by whole cell patch-clamp method. In separate experiments the effect of 4-aminopyridine on resting oocytes was tested. The single-channel patch clamp configuration was employed to assess the electrical response to fertilization with homologous sperm. Structure of oocytes submitted to patch clamp was evaluated with scanning electron microscopy and found to be preserved. Oocyte diameter was 70.2 ±  2.2 µm; their resting parameters were: membrane potential 23.8 ±  0.8 mV; total membrane specific resistance 519.1 ±  94.6 &amp;#937;.cm², and specific capacity 0.99 ±  0.03...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: 4-Aminopyridine; Fertilization; Hamster oocyte; Patch-clamp; Membrane current.
Ano: 2006
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452006000100007

The actin filament network associated to Sertoli cell ectoplasmic specializations
Provedor de dados: 47
Autores: Cavicchia,Juan Carlos; Fóscolo,Mabel; Ibañez,Jorge; Lillig,Christopher; Capani,Francisco.
Junctional devices in Sertoli cells conform the blood-testis barrier and play a key role in maturation and differentiation of germ cells. The spacial distribution of ectoplasmic specializations of Sertoli cells was studied by &amp;beta;-actin immunolabelling, using laser confocal and transmission electron microscopy. For confocal microscopy, &amp;beta;-actin immunolabelling of ectoplasmic specializations was studied over the background of either prosaposin or glutaredoxin immunolabelling of the Sertoli cytoplasm. Labelling was found near the basal lamina, surrounding early spermatocytes (presumably in leptotene-zygotene) or at one of two levels in the seminiferous epithelium: (1) around deep infoldings of the Sertoli cell cytoplasm, in tubular stages...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: &amp;beta; -actin Ectoplasmic specializations Sertoli cell Seminiferous epithelium.
Ano: 2011
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452011000300003

Nuclear pores in luteal cells during pregnancy and after parturition and pup removal in the rat: A freeze-fracture study
Provedor de dados: 47
Autores: Cavicchia,Juan Carlos; Guembe,Gustavo; Fóscolo,Mabel.
In a previous paper we described a pronounced increase of apoptotic nuclei in rat corpus luteum of pregnancy whose programmed chromatin degeneration was induced by the progesterone antagonist mifepristone. Those observations encouraged us to study the apoptotic nuclear membrane during pregnancy and after parturition and pup removal, by using a freeze-fracture technique which allows us to observe 'en face' the nuclear envelop and also permits nuclear pore counting. This study was complemented with the TUNEL assay (TdT-mediated dUTP nick-end labelling). Changes in nuclear pores during pregnancy begin with an intense reduction in number but still showing an even distribution on the nuclear membrane, never forming aggregations sharply separated from pore-free...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apoptosis; Corpus luteum; Nuclear envelope; Nucleocytoplasmic transport.
Ano: 2010
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452010000200004