Consulta: creatorFacets:"Fagundes,Valéria"
Registros recuperados: 8
Data/hora: 09/06/2024 15:35:30

The use of PCR-RFLP as an identification tool for three closely related species of rodents of the genus Akodon (Sigmodontinae, Akodontini)
Provedor de dados: 74
Autores: Fagundes,Valéria; Nogueira,Cristina Dornelas de Andrade.
Three cryptic species of the rodent Akodon (A. cursor, A. montensis and Akodon sp.) were analyzed. The two former species are sympatric in the Brazilian states of São Paulo, Rio de Janeiro and Minas Gerais, where hybrids have already been found. The third species, Akodon sp., occurs in an isolated area in Central Brazil. The identification of these species is difficult by the need of living animals. At present, karyotyping is the only method used in the identification of specimens. We used PCR-RFLP of the mitochondrial cytochrome gene to test the distinctiveness of the three species, which was confirmed by the absence of shared species-specific haplotypes. We also detected a geographical pattern of haplotypes distribution with highly polymorphic...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Akodon cursor; Akodon montensis; PCR-RFLP; Sigmodontinae.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000400031

Noninvasive genetic sampling of endangered muriqui (Primates, Atelidae): efficiency of fecal DNA extraction
Provedor de dados: 74
Autores: Chaves,Paulo B.; Paes,Marcela F.; Mendes,Sérgio L.; Strier,Karen B.; Louro,Iúri D.; Fagundes,Valéria.
The muriqui (Brachyteles) is one of the most endangered primates in the world, however little is known about the viability of the remaining populations. We evaluated the technique of extracting DNA from wild muriqui feces for PCR applications. In order to determine the effect of the DNA in subsequent amplifications, we analyzed five different extracts. The importance of the recommended BSA and the HotStarTaq DNA polymerase was tested. The minimal conditions to successfully amplify highly degraded fecal DNA were determined, showing that the recommended reagents are not required. We envision that this method may be useful in further conservation management studies.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Brachyteles; Conservation genetics; Endangered species; Fecal DNA; Noninvasive sampling.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000400028

Patterns of ribosomal DNA distribution in hylid frogs from the  Hypsiboas faber  and  H. semilineatus  species groups
Provedor de dados: 74
Autores: Nunes,Rosana dos Reis Abrante; Fagundes,Valéria.
We performed a comparative analysis of the nucleolus organizer region (NOR) distribution in the karyotypes of hylid frogs from two different taxonomic groups,  Hypsiboas faber  and  H. semilineatus.  Silver nitrate staining of NORs (Ag-NORs) and fluorescence  in situ  hybridization (FISH) with a rDNA probe were used to investigate the chromosomal location of rDNA loci in two species. The karyotype of  H. semilineatus  and the Ag-NORs distribution of the four species are presented herein for the first time. After conventional staining, the four species presented very similar karyotypes with 2n = 24, but Ag-NORs analyses revealed species-specific characteristics.  H. albomarginatus, H. faber  and  H. semilineatus  had one pair of interstitial Ag-NORs in the...
Palavras-chave: Hylids; Hypsiboas; Karyotypes; FISH; Ag-NORs; Hypsiboas semilineatus; H. faber.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000500028

Testing the Rio Doce as a riverine barrier in shaping the Atlantic rainforest population divergence in the rodent Akodon cursor
Provedor de dados: 74
Autores: Colombi,Victor Hugo; Lopes,Silvia Ramira; Fagundes,Valéria.
Akodon cursor occurs in dense rainforest from northern (8º S) to southern (26º S) states along the Atlantic coast of Brazil. Previous karyological and molecular data revealed two major clades, one including northern (8-15º S) and the other southern (19-26º S) populations. The center of geographic distribution (15-20º S), which included the state of Espírito Santo, was identified as a potential vicariance region. Since river barriers are among the most discussed models in the study of Neotropical diversification, we examined whether the Rio Doce (19º S) plays an important role in shaping the population genetic divergence of A. cursor by including samples from Espírito Santo in the analysis. Our results showed that the northern-southern division region in...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Akodontini; Atlantic Forest; Cytochrome b; Gene flow; Phylogeography; Rodentia.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000400029

Multiple interstitial ribosomal sites (NORs) in the Brazilian squirrel Sciurus aestuans ingrami (Rodentia, Sciuridae) with 2n = 40: an overview of Sciurus cytogenetics
Provedor de dados: 74
Autores: Fagundes,Valéria; Christoff,Alexandre Uarth; Amaro-Ghilard,Renata Cecília; Scheibler,Daniel R.; Yonenaga-Yassuda,Yatiyo.
This is the first time the karyotype of the Brazilian squirrel Sciurusaestuans ingrami, with 2n = 40, is described. The karyotype of this species comprises 18 pairs of biarmed and one minute pair of acrocentric autosomes, a medium-sized submetacentric X and a medium-sized acrocentric Y. Four pairs have an interstitial secondary constriction, co-located with nucleolar organizer regions (NORs), identified by silver-staining technique and fluorescent in situ hybridization (FISH) with ribosomal 18S/28S probes. The occurrence of multiple interstitial NORs is rare in rodents, and this is one of the few examples, identified by a molecular cytogenetics approach.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Squirrels; Chromosomes; Karyotype; NORs; Rodentia.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000300007

Genetic structure in two northern muriqui populations (Brachyteles hypoxanthus, Primates, Atelidae) as inferred from fecal DNA
Provedor de dados: 74
Autores: Fagundes,Valéria; Paes,Marcela F.; Chaves,Paulo B.; Mendes,Sérgio L.; Possamai,Carla de B.; Boubli,Jean P.; Strier,Karen B.
We assessed the genetic diversity of two northern muriqui (Brachyteles hypoxanthus Primata, Atelidae) populations, the Feliciano Miguel Abdala population (FMA, n = 108) in the Brazilian state of Minas Gerais (19°44' S, 41°49' W) and the Santa Maria de Jetibá population (SMJ, n = 18) in the Brazilian state of Espírito Santo (20°01' S, 40°44' W). Fecal DNA was isolated and PCR-RFLP analysis used to analyze 2160 bp of mitochondrial DNA, made up of an 820 bp segment of the gene cytochrome c oxidase subunit 2 (cox2, EC 1.9.3.1), an 880 bp segment of the gene cytochrome b (cytb, EC 1.10.2.2) and 460 bp of the hypervariable segment of the mtDNA control region (HVRI). The cox2 and cytb sequences were monomorphic within and between populations whereas the HVRI...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Brachyteles; Conservation genetics; Fecal DNA; MtDNA; PCR-RFLP.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000100028

Karyology of the Atlantic forest rodent Juliomys (Cricetidae): a new karyotype from southern Brazil
Provedor de dados: 74
Autores: Paresque,Roberta; Christoff,Alexandre Uarth; Fagundes,Valéria.
Juliomys is a small rodent from the family Cricetidae which inhabits the Atlantic forest and forests from Argentina to eastern Brazil. The three species recognized so far have different karyotypes. In this paper, we describe a new karyotype with 2n = 32, FN = 48 found in Juliomys specimens from a high-altitude area in the Atlantic forest of southern Brazil. The karyotype was analyzed after G- and C-banding and silver staining of the nucleolus organizer regions (Ag-NOR) and its G-banding patterns were compared with those of the newly described species Juliomys ossitenuis (2n = 20, FN = 36). The 2n = 32 karyomorph presented peculiar features and was very different from those of the other species of the genus: J. pictipes (2n = 36, FN = 34), J. rimofrons (2n...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Juliomys; Karyotype evolution; J. ossitenuis; Comparative G-banding; Ag-NORs.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000200016

Karyological geographic variation of Oligoryzomys nigripes Olfers, 1818 (Rodentia, Cricetidae) from Brazil
Provedor de dados: 74
Autores: Paresque,Roberta; Silva,Maria José de Jesus; Yonenaga-Yassuda,Yatiyo; Fagundes,Valéria.
The karyotypes of 85 specimens of Oligoryzomys nigripes (Rodentia, Sigmodontinae) collected in the Cerrado and Atlantic Forest of seven states of Brazil were analyzed. Eighty four specimens presented a karyotype with 2n = 62 and one individual had 2n = 61 due to a monosomy of the X chromosome. High levels of intra- and inter-population karyotypic variability, due to sex chromosomes heteromorphisms and pericentric inversions in four autosomes (pairs 2, 3, 4 and 8), led to a variation of the autosomal arm numbers (fundamental number, FN) from 78 to 82. Synaptonemal complex analyses revealed normal meiosis in males heterozygous for pericentric inversions. We found 39 different cytotypes, 27 of which are herein described for the first time. A literature survey...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pericentric inversion; Oryzomyini; Oligoryzomys nigripes; Rodentia; Clinal variation.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000100010