Consulta: creatorFacets:"Feng,Bao Zhen"
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Data/hora: 10/06/2024 10:27:39

Cloning, characterization and expression of a novel laccase gene Pclac2 from Phytophthora capsici
Provedor de dados: 58
Autores: Feng,Bao Zhen; Li,Peiqian.
Laccases are blue copper oxidases (E.C. 1.10.3.2) that catalyze the one-electron oxidation of phenolics, aromatic amines, and other electron-rich substrates with the concomitant reduction of O2 to H2O. A novel laccase gene pclac2 and its corresponding full-length cDNA were cloned and characterized from Phytophthora capsici for the first time. The 1683 bp full-length cDNA of pclac2 encoded a mature laccase protein containing 560 amino acids preceded by a signal peptide of 23 amino acids. The deduced protein sequence of PCLAC2 showed high similarity with other known fungal laccases and contained four copper-binding conserved domains of typical laccase protein. In order to achieve a high level secretion and full activity expression of PCLAC2, expression...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Phytophthora capsici; Laccase; Expression; Purification; Activity.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000100050