Consulta: creatorFacets:"Ferreira,Christina Ramires"
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Data/hora: 06/06/2024 02:36:28

The effects of ovalbumin as a protein source during the in vitro production of bovine embryos
Provedor de dados: 96
Autores: Tetzner,Tatiane Almeida Drummond; Saraiva,Naiara Zoccal; Perecin,Felipe; Niciura,Simone Cristina Méo; Ferreira,Christina Ramires; Oliveira,Clara Slade; Garcia,Joaquim Mansano.
Embryo quality is influenced by the culture conditions that affect in vitro maturation (IVM), fertilization (IVF) and culture (IVC) rates. The present study investigated the feasibility of producing bovine embryos after the replacement of fetal calf serum (FCS) and bovine serum albumin (BSA) by ovalbumin (OVA). The IVM and IVC medium were supplemented with 10% FCS, 4 mg/mL BSA, or 4 mg/mL OVA. The IVF medium was supplemented with 6 mg/mL BSA or OVA. For IVM, supplementation with FCS, BSA, and OVA did not affect nuclear maturation or cortical granule migration. Higher rates of formation of two pronuclei were obtained when FCS was employed for IVM (79.97%), regardless of the supplement used for IVF, and when BSA was used for IVF (59.4%), regardless of the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bovine embryo; In vitro culture; Ovalbumin; Protein source.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982011001000010