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Decellularization of placentas: establishing a protocol
Provedor de dados: 56
Autores: Leonel,L.C.P.C.; Miranda,C.M.F.C.; Coelho,T.M.; Ferreira,G.A.S.; Caãada,R.R.; Miglino,M.A.; Lobo,S.E.
Biological biomaterials for tissue engineering purposes can be produced through tissue and/or organ decellularization. The remaining extracellular matrix (ECM) must be acellular and preserve its proteins and physical features. Placentas are organs of great interest because they are discarded after birth and present large amounts of ECM. Protocols for decellularization are tissue-specific and have not been established for canine placentas yet. This study aimed at analyzing a favorable method for decellularization of maternal and fetal portions of canine placentas. Canine placentas were subjected to ten preliminary tests to analyze the efficacy of parameters such as the type of detergents, freezing temperatures and perfusion. Two protocols were chosen for...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Extracellular matrix; Placenta; Decellularization; Acellular biomaterial.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000100601