Consulta: creatorFacets:"Flachsland,Eduardo Alberto"
Registros recuperados: 1
Data/hora: 11/06/2024 01:30:02

Cryopreservation of Cyrtopodium hatschbachii Pabst (Orchidaceae) immature seeds by encapsulation-dehydration
Provedor de dados: 47
Autores: Surenciski,Mauro Rodrigo; Flachsland,Eduardo Alberto; Terada,Graciela; Mroginski,Luis Amado; Rey,Hebe Yolanda.
The aim of the present study was to investigate the efficiency of the encapsulation-dehydration technique for cryopreservation of Cyrtopodium hastchbachii Pabst seeds. Immature seeds of this species were cryopreserved by an encapsulation-dehydration technique. Seeds of five immature pods, 120 days after pollination, were encapsulated in 3% calcium alginate matrix and pretreated in liquid medium supplemented with 0.08 M sucrose (24 h), 0.15 M sucrose (24 h), 0.25 M sucrose (48 h), 0.5 M sucrose (24 h) and 0.75 M sucrose (24 h) in shaker at 60 rpm. Alginate beads were dehydrated 5 h in silicagel and immersed in liquid nitrogen for 12 h. Cryopreserved beads were thawed at 30°C for 1 min, rehydrated using the same liquid mediums (0.75 M sucrose (24 h), 0.5 M...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cryoconservation; Liquid nitrogen; Calcium alginate; Activated charcoal; Sucrose.
Ano: 2012
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452012000100002