Consulta: creatorFacets:"Freire,R.L."
Registros recuperados: 4
Data/hora: 05/06/2024 02:20:39

Detection of Toxoplasma gondii by PCR and mouse bioassay in commercial cuts of pork from experimentally infected pigs
Provedor de dados: 45
Autores: Tsutsui,V.S.; Freire,R.L.; Garcia,J.L.; Gennari,S.M.; Vieira,D.P.; Marana,E.R.M.; Prudêncio,L.B.; Navarro,I.T.
The distribution of T. gondii in commercial cuts of pork (ham, tenderloin, spareribs and arm picnic) by PCR and bioassay from experimentally infected pigs, was evaluated. Eighteen mixed breed pigs were divided into two groups (G). The G1 animals (n=10) were infected with 4 x10(4) oocysts of the T. gondii VEG strain and the G2 animals (n=8) were used as control. Pigs of both groups were slaughtered at 59th day after infection, and meat samples were collected for bioassay and PCR. All animals from G1 were positive by at least one or both tests, and all control animals were negative. T. gondii was identified in pork by mouse bioassay and PCR in 27/40 (67.5%) and in 9/40 (22.5%) of the evaluated samples, respectively. There were no statistical differences in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pork meat; Mouse bioassay; PCR; Toxoplasma gondii.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352007000100006

Occurrence of antibodies to Neospora caninum and Toxoplasma gondii in dairy cattle from the northern region of the Paraná State, Brazil
Provedor de dados: 45
Autores: Ogawa,L.; Freire,R.L.; Vidotto,O.; Gondim,L.F.P.; Navarro,I.T.
Three-hundred and eighty-five serum samples were taken from dairy cows on 90 farms in 12 counties from the northern region of the Paraná State, Brazil. The samples were analyzed by IFAT for the detection of anti-Neospora caninum and anti-Toxoplasma gondii IgG antibodies. Forty-five (12%) samples were seropositive to N. caninum, while 102 (26%) samples were seropositive to T. gondii. Only four animals were seropositive to both coccidia. No significant difference was observed between the N. caninum serology and any of the variables studied, such as dairy cattle management, milk production, reproductive problems, feeding, and presence of dogs, cats and rodents. These data suggest that neosporosis is present among dairy cattle in the studied geographic region...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dairy cattle; Seroepidemiology; Neospora caninum; Toxoplasma gondii.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352005000300006

Dairy cow abortion associated with Neospora caninum and other infectious agents
Provedor de dados: 45
Autores: Santos,A.P.M.E.; Navarro,I.T.; Bracarense,A.P.F.R.L.; Freire,R.L.; Marana,E.R.M.; Ogawa,L.; Alfieri,A.A.; Freitas,J.C.; Vidotto,O.
Trinta e cinco vacas de rebanhos leiteiros da região Norte do estado do Paraná, com histórico de abortamento, foram pesquisadas sorologicamente para verificar a presença de anticorpos contra Neospora caninum, Toxoplasma gondii, Leptospira spp., Brucella abortus, BHV-1 e BVDV. Vinte e uma vacas apresentaram títulos sorológicos compatíveis com infecção. Todas elas, soropositivas para N. caninum, foram também soropositivas para outros agentes infecciosos, sugerindo a possibilidade de associação desses agentes nos problemas reprodutivos de bovinos, no estado do Paraná.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cattle; Abortion; Neospora caninum; Reproductive disorder.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352005000400017

Vaccination of pigs with Toxoplasma gondii antigens incorporated in immunostimulating complexes (iscoms)
Provedor de dados: 45
Autores: Freire,R.L.; Navarro,I.T.; Bracarense,A.P.F.R.L.; Gennari,S.M.
Immunity to Toxoplasma gondii was studied in pigs, after vaccination with T. gondii antigens incorporated into immunostimulating complexes. Nine pigs (group 1 - G1) were inoculated subcutaneously with T. gondii iscoms (LIV-5 sample) and three doses were given at 21 and 13 day-intervals. The results were compared in other three groups of nine pigs each: animals in group 2 (G2) were immunized with the LIV-5 antigens without Quil A, animals in group 3 (G3) were inoculated with tachyzoites of RH T. gondii isolate, and animals in group 4 (G4) received no vaccination. Four animals were neither vaccinated nor challenged with T. gondii (group 5 - G5). Thirty days after vaccination, pigs were challenged orally with 5´10(4) oocysts at AS-28 T. gondii isolate....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Toxoplasma gondii; Pig; Vaccine; Iscom.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352003000400002