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Optimization of phenoxazinone synthase production by response surface methodology and its application in Congo red decolourization
Provedor de dados: 69
Autores: Jia,Honghua; Gao,Zhen; Ma,Yingying; Zhong,Chao; Xie,Yingchun; Zhou,Hua; Wei,Ping.
Background: Enzymatic decolourization has been recently proposed as a promising and eco-friendly method for treatment of synthetic dye-contaminated wastewaters. However, the processes require large quantities of enzymes, attracting significant attention in developing efficient methods for mass production of multifunctional enzymes. Several methods such as response surface methodology (RSM) and orthogonal experiment have been applied to optimize the parameters in bioprocesses for enzyme production. Results: In the present study, a laccase-like enzyme, phenoxazinone synthase (PHS) originated from Streptomyces antibioticus was recombinantly expressed in Escherichia coli BL21 (DE3). The production of PHS in E. coli BL21 was optimized by response surface...
Tipo: Journal article
Palavras-chave: Congo red; Decolourization; Laccase; Phenoxazinone synthase; Response surface methodology.
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000500002