Consulta: creatorFacets:"Garcia,Raycon Roberto Freitas"
Registros recuperados: 2
Data/hora: 12/06/2024 15:32:18

Functional integrity of Colossoma macropomum (Cuvier, 1816) sperm cryopreserved with enriched extender solutions
Provedor de dados: 83
Autores: Garcia,Raycon Roberto Freitas; Vasconcelos,Ana Carina Nogueira; Povh,Jayme Aparecido; Oberst,Eneder Rosana; Varela Jr.,Antonio Sérgio; Corcini,Carine Dahl; Streit Jr.,Danilo Pedro.
Cryoprotectant solutions are used to protect the sperm from alterations caused by the low temperature in the cryopreservation process. We evaluated the quality of Colossoma macropomum semen after freezing, using dimethyl sulfoxide (DMSO) as a cryoprotectant, combined with two extender solutions (T1 - Solution 1: Glucose 90.0 g/L, Sodium Citrate 6.0 g/L, EDTA 1.5 g/L, Sodium Bicarbonate 1.5 g/L, Potassium Chloride 0.8 g/L, Gentamycin Sulphate 0.2 g/L, and T2 - Solution 2: Glucose 90.0 g/L, ACP(r)-104 10.0 g/L). Motility rate and motility time did not differ between T1 and T2 and were lower than fresh semen. The number of normal sperm was significantly different in treatments T1 (15.1%) and T2 (21.9%), and both showed a reduction in the percentage of normal...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cryopreservation; Cryoprotectant solution; Mitochondrial functionality; Motility; Spermatic DNA.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252015000300599

Different extenders solutions for tambaqui semen cooling
Provedor de dados: 86
Autores: Garcia,Raycon Roberto Freitas; Vasconcelos,Ana Carina Nogueira; Povh,Jayme Aparecido; Oberst,Eneder Rosana; Eloy,Lidiane Raquel; Streit Junior,Danilo Pedro.
Abstract: The objective of this work was to evaluate the efficiency of extenders solutions in tambaqui (Colossoma macropomum) semen cooling at 6°C. The treatments consisted of semen dilutions with three extenders - BTS, HBSS, and ACP-104 -, and a control with undiluted semen. Cooled semen was evaluated by 24, 48, 72, 96, and 120 hours. There were differences for the duration of motility and fertilization rates among the semen dilutions. Motility duration proved a highly variable evaluation after 72 hours. Dilution testing can be performed by the three products, and cooling storage for 72 hours is most effective with BTS extender.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Colossoma macropomum; ACP; BTS; HBSS; Motility; Sperm.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2016000600780