Consulta: creatorFacets:"Geffroy, Solene"
Registros recuperados: 2
Data/hora: 10/06/2024 14:15:15

From the sxtA4 Gene to Saxitoxin Production: What Controls the Variability Among Alexandrium minutum and Alexandrium pacificum Strains?
Provedor de dados: 5
Autores: Geffroy, Solene; Lechat, Marc-marie; Le Gac, Mickael; Rovillon, Georges-augustin; Marie, Dominique; Bigeard, Estelle; Malo, Florent; Amzil, Zouher; Guillou, Laure; Caruana, Amandine.
Paralytic shellfish poisoning (PSP) is a human foodborne syndrome caused by the consumption of shellfish that accumulate paralytic shellfish toxins (PSTs, saxitoxin group). In PST-producing dinoflagellates such as Alexandrium spp., toxin synthesis is encoded in the nuclear genome via a gene cluster (sxt). Toxin production is supposedly associated with the presence of a 4th domain in the sxtA gene (sxtA4), one of the core genes of the PST gene cluster. It is postulated that gene expression in dinoflagellates is partially constitutive, with both transcriptional and post-transcriptional processes potentially co-occurring. Therefore, gene structure and expression mode are two important features to explore in order to fully understand toxin production processes...
Tipo: Text
Palavras-chave: Alexandrium; Saxitoxins; SxtA4; Copy number variation; SxtA; Expression; Isoform.
Ano: 2021
URL: https://archimer.ifremer.fr/doc/00682/79402/81944.pdf

Simplified Transformation of Ostreococcus tauri Using Polyethylene Glycol
Provedor de dados: 5
Autores: Sanchez, Frédéric; Geffroy, Solene; Norest, Manon; Yau, Sheree; Moreau, Hervé; Grimsley, Nigel.
Ostreococcus tauri is an easily cultured representative of unicellular algae (class Mamiellophyceae) that abound in oceans worldwide. Eight complete 13–22 Mb genomes of phylogenetically divergent species within this class are available, and their DNA sequences are nearly always present in metagenomic data produced from marine samples. Here we describe a simplified and robust transformation protocol for the smallest of these algae (O. tauri). Polyethylene glycol (PEG) treatment was much more efficient than the previously described electroporation protocol. Short (2 min or less) incubation times in PEG gave &gt;104 transformants per microgram DNA. The time of cell recovery after transformation could be reduced to a few hours, permitting the experiment to be...
Tipo: Text
Palavras-chave: Chlorophyta; Prasinophyte; Mamiellophyceae; Ostreococcus; Plankton; Picoeukaryote; Ecosystem; Bioluminescence; Promoter; Luciferase; Gene expression.
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00500/61170/64639.pdf