Consulta: creatorFacets:"Godin, Pierre"
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Data/hora: 11/06/2024 09:35:12

Localisation and characterization of acid phosphate activity in Bonamia ostreae (Ascetospora), an intrahemocytic protozoan parasite of the flat oyster Ostrea edulis (Bivalvia)
Provedor de dados: 5
Autores: Hervio, Dominique; Chagot, Dominique; Godin, Pierre; Grizel, Henri; Mialhe, Eric.
Acid phosphatase activity was cytochemically detected at the ultrastructural level in the protozoan Bonamia ostreae, an intrahemocytic parasite of the flat oyster Ostrea edulis. Lead nitrate was used as capture agent for phosphate liberated during the hydrolysis of the subtrate (cytidine 5' monophosphate). Lead phosphate precipiation, indicative of enzyme activity, was located in membrane-bound organelles corresponding to the cytoplasmic inclusions known as "dense bodies". Acid phosphatase activity level and sodium L-tartrate inhibition were measured spectrophotometrically from a purified parasite suspension. The possible involvement of this enzyme in intracellular survival of B. ostrea is discussed.
Tipo: Text
Palavras-chave: Acid phosphate activity; Ostrea edulis; Bonamia ostreae.
Ano: 1991
URL: http://archimer.ifremer.fr/doc/1991/publication-2755.pdf