Consulta: creatorFacets:"González, Wendy"
Registros recuperados: 1
Data/hora: 11/06/2024 11:15:38

Gamete quality and management for in vitro fertilisation in meagre (Argyrosomus regius)
Provedor de dados: 5
Autores: Ramos-júdez, Sandra; González, Wendy; Dutto, Gilbert; Mylonas, Constantinos C.; Fauvel, Christian; Duncan, Neil.
The aquaculture of meagre (Argyrosomus regius) requires methods for the control of reproduction that enable the production of families from specific individuals for selective breeding programs. We experimentally determined the parameters required for an in vitro fertilisation protocol. A total of 14 females and 5 males (mean ± S.D. weights of 20.45 ± 6.22 and 15.94 ± 2.75 kg, respectively) were used. Selected females had vitellogenic oocytes &gt;550 μm in diameter and males had fluid sperm upon application of abdominal pressure. Both sexes were treated with an injection of 15 μg kg−1 of gonadotropin-releasing hormone agonist (GnRHa) to induce oocyte maturation/ovulation and enhance sperm production. To determine the timing of ovulation and window of high...
Tipo: Text
Palavras-chave: Argyrosomus regius; Meagre; Reproduction; Artificial fertilisation; Gamete management; GnRHa.
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00495/60682/64178.pdf