Consulta: creatorFacets:"Guembarovski,Roberta Losi"
Registros recuperados: 4
Data/hora: 06/06/2024 11:27:54

Involvement of transforming growth factor beta-1 (TGFβ1) cytokine and FOXP3 transcription factor genetic polymorphisms in hematological malignancies
Provedor de dados: 52
Autores: Vitiello,Glauco Akelinghton Freire; Guembarovski,Roberta Losi; Oliveira,Carlos Eduardo Coral de; Amarante,Marla Karine; Perim,Aparecida de Lourdes; Watanabe,Maria Angelica Ehara.
Hematological malignancies (HM) are a group of neoplastic diseases that arise from hematologic cell lineages. Transforming growth factor beta 1 (TGFβ1) is shown to negatively regulate normal and malignant hematopoiesis and, in immunological context, to promote T cell exhaustion and generation of regulatory T cells, which are shown to be deleterious in cancer, by the induction of transcription factor FOXP3 expression. The present study aimed to evaluate TGFB1 exon-1 rs1800470 and FOXP3 intron-1 rs2232365 polymorphisms in relation to HM susceptibility. DNA was extracted from blood samples of 43 HM patients and 142 neoplasia-free individuals and polymorphisms were analyzed by allelic-specific PCR. Association analysis was assessed by the Odds Ratio (OR) with...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hematological malignancies; TGFB1; FOXP3; Genetic polymorphism; Susceptibility.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000400553

Protein Expression and Codon 72 Polymorphism of TP53 Gene in Triple Negative Breast Cancer
Provedor de dados: 52
Autores: Lopes,Leandra Fiori; Guembarovski,Roberta Losi; Guembarovski,Alda Losi; Kishima,Marina Okuyama; Campos,Clodoaldo Zago; Derossi,Daniela Rudgeri; Ariza,Carolina Batista; Ozawa,Patricia Midori Murobushi; Oliveira,Carlos Eduardo Coral de; Banin-Hirata,Bruna Karina; Vitiello,Glauco Akelinghton Freire; Borelli,Sueli Donizete; Watanabe,Maria Angelica Ehara.
A subgroup of tumor that has received attention is triple-negative breast cancer (TNBC), which presents phenotype of negative estrogen receptor, negative progesterone receptor and has no overexpression of HER2. TP53 acts as a tumor suppressor limiting the proliferation of damaged cells. A polymorphic site (rs1042522) of TP53 encodes either an arginine or a proline amino acid, but its biological significance remains unclear. This study aimed to investigate this variant and its expression in search for a possible involvement in TNBC susceptibility and clinical outcome. Genetic polymorphism was evaluated in 50 patients and 115 controls by PCR based methodology and immunohistochemistry was done with monoclonal antibody. Case-control study showed no positive or...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Breast cancer; TNBC; TP53; Genetic polymorphism; Immunohistochemistry.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000600895

Identification of a splicing coactivator gene that affects the production of ochratoxin a in Aspergillus carbonarius
Provedor de dados: 52
Autores: Lunardi,Lígia Uno; Guembarovski,Roberta Losi; Hanai,Luiz Ricardo; Cristiano,Valderi; Vieira,Maria Lucia Carneiro; Sartori,Daniele; Fungaro,Maria Helena Pelegrinelli.
Ochratoxin A is a mycotoxin produced by some fungi species. Among them, Aspergillus carbonarius is considered a powerful producer. Genes involved in the ochratoxin A biosynthesis pathway have been identified in some producer species. However, there are few studies that purpose to identify these genes in A. carbonarius. The use of insertion mutants to identify genes associated with certain properties has been increased in the literature. In this work, the region of T-DNA integration was investigated in one A. carbonarius ochratoxin-defective mutant previously obtained by Agrobacterium tumefaciens-mediated transformation, in order to find an association between interrupted gene and the biosynthesis of ochratoxin A. The integration occurred in a gene that...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ochratoxigenic fungi; Mycotoxin; Real time PCR; Splicing coactivator protein.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000700018

TT virus in peripheral blood cells from patients with human papillomavirus (HPV): investigating association with cervical carcinoma
Provedor de dados: 52
Autores: Suzuki,Patrícia Sayuri; Oliveira,Karen Brajão de; Herrera,Ana Cristina do Amaral; Borelli,Sueli Donizete; Guembarovski,Roberta Losi; Oliveira,Carlos Eduardo Coral de; Oda,Julie Massayo Maeda; Ozawa,Patricia Midori Murobushi; Watanabe,Maria Angelica Ehara.
Torque Teno Virus (TTV) presence was investigated in peripheral blood of 117 brazilian women by nested polymerase chain reaction. TTV DNA was observed in 18.6% of healthy donors and in 24.32% Human Papillomavirus (HPV) patients. TTV presence was also investigated in the HPV positive group for comparison between the cervical cancer and noncancerous patients. TTV DNA prevalence was significantly higher among the HPV positive patients with cervical cancer (57.14%) than in HPV noncancerous patients (16.67%). Thus, the presence of TTV infection could be a risk factor for cancer development in the patients presenting HPV-TTV coinfection. Further studies are required to clarify the TTV influence in HPV pathogenesis.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: TTV DNA; HPV; Cervical cancer.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000200010