Consulta: creatorFacets:"Guitton, Yann"
Registros recuperados: 11
Data/hora: 11/06/2024 02:29:48

Cytotoxicity, Fractionation and Dereplication of Extracts of the Dinoflagellate Vulcanodinium rugosum, a Producer of Pinnatoxin G
Provedor de dados: 5
Autores: Geiger, Marie; Desanglois, Gwenaelle; Hogeveen, Kevin; Fessard, Valerie; Lepretre, Thomas; Mondeguer, Florence; Guitton, Yann; Herve, Fabienne; Sechet, Veronique; Grovel, Olivier; Pouchus, Yves-francois; Hess, Philipp.
Pinnatoxin G (PnTX-G) is a marine toxin belonging to the class of cyclic imines and produced by the dinoflagellate Vulcanodinium rugosum. In spite of its strong toxicity to mice, leading to the classification of pinnatoxins into the class of “fast-acting toxins”, its hazard for human health has never been demonstrated. In this study, crude extracts of V. rugosum exhibited significant cytotoxicity against Neuro2A and KB cells. IC50 values of 0.38 µg mL−1 and 0.19 µg mL−1 were estimated on Neuro2A cells after only 24 h of incubation and on KB cells after 72 h of incubation, respectively. In the case of Caco-2 cells 48 h after exposure, the crude extract of V. rugosum induced cell cycle arrest accompanied by a dramatic increase in double strand DNA breaks,...
Tipo: Text
Palavras-chave: Dereplication; Cyclic imine; HRMS; Bioactivity; Pinnatoxins.
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00156/26723/24802.pdf

Maldi mass spectrometry imaging applied to small molecules localization
Provedor de dados: 5
Autores: Guitton, Yann; Mondeguer, Florence; Nicolau, Elodie; Royer, Florence; Hilbert, Jean-louis; Fournier, Isabelle; Wisztorski, Maxence.
MALDI Mass Spectrometry Imaging (MALDI-MSI) is a two-dimensional MALDI mass spectrometric technique used to visualize the spatial distribution of a large variety of biological molecules (from small metabolites to large proteins) without extraction, purification, separation or labelling of biological samples. Since its introduction by Caprioli et al in 1997, it has become one of the most important molecular histology methods for biomarker hunting and for understanding the spatial distribution of biomolecules in various tissues. MALDI-MSI is thus ideal for complementing the expanding field of metabolomics. Despite the fact that MALDI-MSI have been very successfully applied in divers studies (even in clinical applications) its use remains far from routine,...
Tipo: Text
Ano: 2012
URL: http://archimer.ifremer.fr/doc/00179/29041/27479.pdf

COLNACOQ. Composés lipophiles naturels dans l’environnement des coquillages. Rapport final
Provedor de dados: 5
Autores: Hess, Philipp; Geiger, Marie; Brochard, Solène; Lepretre, Thomas; Fessard, Valerie; Antignac, Jean-philippe; Dupont, Jacques; Sechet, Veronique; Vanel, Faustine; Mondeguer, Florence; Herve, Fabienne; Leborgne, Sabrina; Deslanglois, Gwenaëlle; Marshall, Lindsey; Rounds, Lucy; Guitton, Yann; Amzil, Zouher; Grovel, Olivier; Ruiz, Nicolas; Pouchus, Francois.
Cette étude s’est inscrite dans un contexte d’évaluation de la salubrité des mollusques bivalves destinés à la consommation humaine. Ces organismes peuvent en effet accumuler des toxines, en particulier de microalgues, en concentrations importantes, pouvant induire des intoxications chez les personnes les consommant. Afin d’assurer la protection des consommateurs, les toxines réglementées sont recherchées dans les coquillages par techniques physico-chimiques. En parallèle, les toxines émergentes, potentiellement produites par d’autres organismes que les micro-algues telles que les micromycètes, peuvent être detectées par l’utilisation du test de toxicité aigüe sur souris. Cependant, ce bio-essai présente de très nombreux inconvénients, notamment éthiques...
Tipo: Text
Palavras-chave: Cytotoxicité; Toxicité sur larves de diptères; Activité antibactérienne; Bactéries marines; Toxines lipophiles; Micro-algues; Dinoflagellés; Micromycètes; Penicillium; Acide okadaïque; Azaspiracide; Pinnatoxine G; Vulcanodinium rugosum; Prorocentrum lima; Azadinium spinosum; Beauveria brongniartii.
Ano: 2015
URL: http://archimer.ifremer.fr/doc/00253/36470/35015.pdf

Stratégie de mesure non ciblée de type métabolomique (couplage LC-HRMS ) pour identifier différents groupes de composés bioactifs accumulés dans les mollusques bivalves
Provedor de dados: 5
Autores: Mondeguer, Florence; Antignac, Jean-philippe; Guitton, Yann; Monteau, Fabrice; Le Borgne, Sabrina; Hess, Philipp.
La bioactivité des toxines du phytoplancton qui s’accumulent dans les coquillages est presque toujours évaluée sur modèle animal (test souris). Malgré ses avantages, la capacité de ce test à expliquer la nature de cette bio-activité reste limitée. Par ailleurs, le contrôle sanitaire actuellement basé sur une méthode ciblée d’identification et de quantification d’un ensemble de toxines connues ne permet pas de détecter des toxines encore inconnues. Afin de répondre à ce besoin de caractérisation de substances toxiques inconnues, une nouvelle approche de profilage chimique différentiel et non ciblé, de type métabolomique, a été proposée. Les 2 extraits sélectionnés sont ceux ayant montré une toxicité positive chez la souris, sans que les substances...
Tipo: Text
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00179/29042/27480.pdf

First insight on metabolomics workflows and differences between dinoflagellate: characterization and discrimination of modern and ancient strains and their algal growth
Provedor de dados: 5
Autores: Mondeguer, Florence; Souard, Florence; Guitton, Yann; Latimier, Marie; Quere, Julien; Sibat, Manoella; Hess, Philipp; Siano, Raffaele.
Aquatic ecosystems worldwide have been negatively affected by eutrophication, many of them driven by increasing nutrient inputs from untreated domestic sewage and industrial and agricultural wastewater. During their life cycle, Alexandrium minutum and Scrippsiella donghaienis can produce resistant and revivable cysts that can accumulate in the sediments of the coastal marine environment for hundreds of years. As a working hypothesis, we presumed that these two genera have preserved their adaptation to such ecosystem changes in the form of cysts in the old sediments. From revivified cysts, some cultures of toxic and non-toxic dinoflagellates (A. minutum and S. donghaienis) were analyzed by a untargeted metabolomics approach by LC-HRMS to study the possible...
Tipo: Text
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00440/55199/56664.pdf

HaloSeeker v1.0, a user-friendly software application for screening halogenated chemicals from untargeted high resolution mass spectrometry data
Provedor de dados: 5
Autores: Cariou, Ronan; Léon, Alexis; Hutinet, Sébastien; Guitton, Yann; Hurel, J; Pourchet-gellez, M; Antignac, Jean Philippe; Munschy, Catherine; Tixier, Celine; Dervilly-pinel, Gaud; Le Bizec, Bruno.
Tipo: Text
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00610/72172/70943.pdf

Innovative metabolomic workflows to study growth kinetics dinoflagellate cysts revived from modern and ancient sediments
Provedor de dados: 5
Autores: Mondeguer, Florence; Souard, Florence; Guitton, Yann; Allard, Pierre-marie; Elie, Nicolas; Sibat, Manoella; Latimier, Marie; Quere, Julien; Wolfender, Jean-luc; Siano, Raffaele; Hess, Philipp.
Aquatic ecosystems worldwide have been negatively affected by eutrophication, many of them driven by increasing nutrient inputs from untreated domestic sewage and industrial and agricultural wastewater. During their life cycle, Alexandrium minutum and Scrippsiella donghaienis can produce resistant and revivable cysts that can accumulate in the sediments of the coastal marine environment for hundreds of years. As a working hypothesis, we presumed that these two genera have preserved their adaptation to such ecosystem changes in the form of cysts in the old sediments. From revivified cysts, some cultures of toxic and non-toxic dinoflagellates (A. minutum and S. donghaienis) were analyzed by a metabolomic approach to study the possible adaptive responses of...
Tipo: Text
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00464/57591/59769.pdf

Nouvelle stratégie de caractérisation non ciblée de type métabolomique au service de l’identification de composés bioactifs accumulés dans les mollusques bivalves
Provedor de dados: 5
Autores: Mondeguer, Florence; Antignac, Jean-philippe; Guitton, Yann; Monteau, Fabrice; Le Borgne, Sabrina; Hess, Philipp.
The bioactivity of phytoplankton toxins that accumulate in shellfish is almost always tested on animal model (mice assay). In spite of these advantages, the ability of this test to explain the nature of the bioactivity remains limited. Besides, the current sanitary control based on targeted methods of identification and quantification (LC-MS/MS) of known toxins, do not permit to detect unknown toxins. In order to cope with this need of identification of unknown toxic substances, a next approach based on global and differential metabolomic profiling was proposed. The first results obtained from extracts that have shown a positive toxicity in mice without the substances potentially responsible for these toxic effects have been measured by targeted methods,...
Tipo: Text
Palavras-chave: Métabolomique; LC-HRMS; Biotoxines marines; Biomarqueur.
Ano: 2012
URL: http://archimer.ifremer.fr/doc/00077/18784/16352.pdf

Cytotoxicity and mycotoxin production of shellfish-derived Penicillium spp., a risk for shellfish consumers
Provedor de dados: 5
Autores: Geiger, Marie; Guitton, Yann; Vansteelandt, M.; Kerzaon, I.; Blanchet, Estelle; Du Pont, T. Robiou; Frisvad, J. C.; Hess, Philipp; Pouchus, Y. F.; Grovel, Olivier.
In order to assess the putative toxigenic risk associated with the presence of fungal strains in shellfish-farming areas, Penicillium strains were isolated from bivalve molluscs and from the surrounding environment, and the influence of the sample origin on the cytotoxicity of the extracts was evaluated. Extracts obtained from shellfish-derived Penicillia exhibited higher cytotoxicity than the others. Ten of these strains were grown on various media including a medium based on mussel extract (Mytilus edulis), mussel flesh-based medium (MES), to study the influence of the mussel flesh on the production of cytotoxic compounds. The MES host-derived medium was created substituting the yeast extract of YES medium by an aqueous extract of mussel tissues, with...
Tipo: Text
Palavras-chave: Cytotoxicity; Dereplication; Marine fungi; Mass spectrometry; Penicillium; Shellfish.
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00160/27166/25523.pdf

Workflows novateurs pour une analyse métabolomique plus claire et plus intégrative
Provedor de dados: 5
Autores: Mondeguer, Florence; Allard, Pierre-marie; Souard, Florence; Elie, Nicolas; Guitton, Yann; Sibat, Manoella; Siano, Raffaele.
During their life cycle, Alexandrium minutum and Scrippsiella donghaienis can produce resistant and revivable cysts that can accumulate in marine sediments for hundreds of years. Our working hypothesis assumes that these two genera have "recorded" their adaptation to ecosystem changes in these cells preserved in the form of cysts. From these revived cysts, toxic and non-toxic dinoflagellate cultures (Alexandrium minutum and Scrippsiella donghaienis) were analyzed by a metabolomic approach to investigate the possible adaptive responses of phytoplankton to these changes in coastal ecosystems. The strains were obtained from isotopically dated sediments, 1986 and 1996 for "old" and 2006 for "modern". In total, 84 samples were obtained. Each experimental...
Tipo: Text
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00485/59662/62721.pdf

Sélection de workflows adaptés à une analyse métabolomique plus transparente et intégrative
Provedor de dados: 5
Autores: Mondeguer, Florence; Allard, Pierre-marie; Souard, Florence; Elie, Nicolas; Guitton, Yann; Sibat, Manoella; Siano, Raffaele.
During their life cycle, Alexandrium minutum and Scrippsiella donghaienis can produce resistant and revivable cysts that can accumulate in marine sediments for hundreds of years. Our working hypothesis assumes that these two genera have "recorded" their adaptation to ecosystem changes in these cells preserved in the form of cysts. From these revived cysts, toxic and non-toxic dinoflagellate cultures (Alexandrium minutum and Scrippsiella donghaienis) were analyzed by a metabolomic approach to investigate the possible adaptive responses of phytoplankton to these changes in coastal ecosystems. The strains were obtained from isotopically dated sediments, 1986 and 1996 for "ancient" and 2006 for "modern". In total, 84 samples were obtained. Each experimental...
Tipo: Text
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00498/60941/64339.pdf