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Analysis of gene expression in Kalanchoe daigremontiana leaves during plantlet formation under drought stress
Provedor de dados: 69
Autores: Zhong,Tianxiu; Zhu,Chen; Zeng,Huiming; Han,Liebao.
Background: Kalanchoe daigremontiana is an attractive model system for the study of the molecular mechanisms of somatic embryogenesis and organogenesis competence due to its formation of plantlets with adventitious roots on the leaf margins that are derived from somatic embryos. The suppression subtractive hybridization technique was used to investigate gene expression during asexual reproduction. Leaves from plants subjected to drought stress provided the source of &amp;#8216;Tester&amp;#8217; DNA, and leaves from plants grown under normal conditions provided the &amp;#8216;Driver&amp;#8217; DNA for subtractive hybridization. Results: A total of 481 high quality ESTs were generated, which clustered into 390 unigenes. Of these unigenes, 132 grouped into 12...
Tipo: Journal article
Palavras-chave: Bioinformatics analysis; Real time PCR; Suppression subtractive hybridization.
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000600004