Consulta: creatorFacets:"Hanquet, Anne-caroline"
Registros recuperados: 2
Data/hora: 06/06/2024 10:49:27

Cryopreservation of Crassostrea gigas vesicular cells: Viability and metabolic activity
Provedor de dados: 5
Autores: Hanquet, Anne-caroline; Kellner, K; Heude, C; Naimi, Amine; Mathieu, M; Poncet, J.m.
Cryopreservation is widely used for long-term conservation of various tissues, embryos or gametes. However, few studies have described cryopreservation of invertebrate primary cell cultures and more particularly of marine invertebrate somatic cells. This technique would however be of great interest to facilitate the study of various metabolic processes which vary seasonally. The aim of the present study was to develop a protocol for cryopreservation of Crassostrea gigas vesicular cells. Different parameters were adjusted to improve recovery of cells after freezing. The most efficient cryoprotectant agent was a mix of Me2SO, glycerol, and ethylene glycol (4% each). The optimal cooling rate was -1 degrees C min(-1) down to -70 degrees C before transfer into...
Tipo: Text
Palavras-chave: Glycogen metabolism; Vesicular cells; Cryopreservation; Mollusc; Oyster; Crassostrea gigas.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/publication-1938.pdf

Approches cellulaires et moléculaires de la gestion des réserves chez l’huître, Crassostrea gigas (Thunberg, 1793). Suivi saisonnier du métabolisme énergétique et effet du niveau trophique sur la mise en réserve
Provedor de dados: 5
Autores: Hanquet, Anne-caroline.
In the Pacific oyster, Crassostrea gigas, the energy necessary for general metabolism and gametogenesis is mainly provided by the glycogen stored in the vesicular cells. The first level of glycogen metabolism control is the entrance of glucose into cells. The monitoring of the uptake capacity of [U-14C] D-glucose revealed the existence of a saturable component with kinetic constants: Km = 0.71 mM ; Vmax = 0.22 nmol/h/106cells. The use of inhibitors has revealed the presence of carriers belonged to GLuT and SGLT types. A follow-up showed a seasonal variation of the uptake capacity and incorporation of glucose into glycogen, and a high expression of SGLT during mobilization of glycogen and gametes maturation. A bioassay in a controlled rearing system has...
Tipo: Text
Palavras-chave: Crassostrea gigas; Glucides métabolisme; Glucose; Reproduction; Cryoconservation; Crassostrea gigas; Carbohydrate metabolism; Glucose; Reproduction; Cryoconservation.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/00013/12399/9185.pdf