Consulta: creatorFacets:"Hara, Olgga A"
Registros recuperados: 1
Data/hora: 11/06/2024 12:11:01

Expression of recombinant dense granule protein 7 of Neospora caninum and evaluation of its diagnostic potential for canine neosporosis
Provedor de dados: 19
Autores: Hara, Olgga A; Liao, Min; Baticados, Waren; Bannai, Hiroshi; Zhang, Guohong; Zhang, Sofa; Lee, Eung-goo; Nishikawa, Yoshifumi; Claveria, Florencia G.; Igarashi, Makoto; Nagasawa, Hideyuki; Xuan, Xuenan.
Neospora caninum infection is an important disease affecting bovine and canine populations worldwide. The dense granule antigen 7 of N. caninum (NcGRA7) is considered to be an immunodominant antigen. In the present study, the gene encoding truncated NcGRA7 (NcGRA7t) lacking an N-terminal signal peptide was expressed in Escherichia coli, and its diagnostic potential in an enzyme-linked immunosorbent assay (ELISA) was evaluated. The ELISA could clearly discriminate between known N. caninum-positive and -negative sera from dogs. Serum samples randomly collected from dogs in Japan and China were examined for the diagnosis of neosporosis using the ELISA. Twenty-three of 135 samples (17.03%) and 9 of 95 (9.47%) samples from Japan and China, respectively, were...
Palavras-chave: Canine; ELISA; Neospora caninum; NcGRA7.
Ano: 2006
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/995