Consulta: creatorFacets:"Harrang, Estelle"
Registros recuperados: 8
Data/hora: 11/06/2024 11:10:36

Fragmentation des populations naturelles d'Ostrea edulis: une adaptation locale de l'huître plate européenne
Provedor de dados: 5
Autores: Harrang, Estelle; Bierne, Nicolas; Heurtebise, Serge; Morga, Benjamin; Lapegue, Sylvie.
L'huître plate européenne Ostrea edulis a connu une période faste dans l'aquaculture jusqu'à la fin des années 1960, lorsque deux parasites, Marteilia refringens (1969) et Bonamia ostreae (1979), ont successivement décimé les bancs naturels de cette espèce endémique, déjà affaiblis par la sur-exploitation de ses gisements. Historiquement présente sur toute la côte européenne, de la Mer du Nord à la Mer Méditerranée, en passant par l'Océan Atlantique, la Mer Adriatique et la Mer Noire, les bancs naturels de cette espèce sont devenus très localisés, laissant penser à des phénomènes d'adaptation locale de l'huître vis à vis de son environnement biotique et abiotique.  La diversité et la structuration génétique de plusieurs populations de l'espèce, à l'échelle...
Tipo: Text
Palavras-chave: Huître plate; Ostrea edulis; Génétique des populations; Sélection Génétique.
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00014/12547/9417.pdf

Bilan des productions expérimentales d'huîtres plates de 2005 à 2010 au Laboratoire de Génétique et Pathologie de La Tremblade
Provedor de dados: 5
Autores: Heurtebise, Serge; Harrang, Estelle; Lallias, Delphine; Lapegue, Sylvie.
L’objectif de ce rapport est de détailler les productions d’huîtres plates européennes, Ostrea edulis, dans le cadre des projets de recherche développés autour de l’amélioration génétique de cette espèce. Nous avons choisi de synthétiser les actions de recherche dans un premier temps puis la production des huîtres et les expérimentations dans un ordre chronologique.
Tipo: Text
Palavras-chave: Huître plate; Ostrea edulis; Production expérimentale; Amélioration génétique.
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00013/12421/9211.pdf

Can survival of European flat oysters following experimental infection with Bonamia ostreae be predicted using QTLs?
Provedor de dados: 5
Autores: Harrang, Estelle; Heurtebise, Serge; Faury, Nicole; Robert, Maeva; Arzul, Isabelle; Lapegue, Sylvie.
The present study identifies quantitative trait loci (QTLs) in response to an experimental infection with the parasite responsible for bonamiosis, Bonamia ostreae, in two segregating families of the European flat oyster, Ostrea edulis. We first constructed a genetic-linkage map for each studied family and improved the existing genetic-linkage map for the European flat oyster with a set of SNP markers. This latter map now combines the best accuracy and the best estimate of the genome coverage available for an oyster species. Secondly, by comparing the QTLs detected in this study with those previously published for O. edulis in similar experimental conditions, we identified several potential QTLs that were identical between the different families, and also...
Tipo: Text
Palavras-chave: Ostrea edulis; Single Nucleotide Polymorphism; Disease resistance; Genetic and QTL mapping; Candidate genes.
Ano: 2015
URL: http://archimer.ifremer.fr/doc/00271/38244/36429.pdf

A high load of non-neutral amino-acid polymorphisms explains high protein diversity despite moderate effective population size in a marine bivalve with sweepstakes reproduction
Provedor de dados: 5
Autores: Harrang, Estelle; Lapegue, Sylvie; Morga, Benjamin; Bierne, Nicolas.
Marine bivalves show among the greatest allozyme diversity ever reported in Eukaryotes, putting them historically at the heart of the neutralist-selectionist controversy on the maintenance of genetic variation. Although it is now acknowledged that this high diversity is most probably a simple consequence of a large population size, convincing support for this explanation would require a rigorous assessment of the silent nucleotide diversity in natural populations of marine bivalves, which has not yet been done. This study investigated DNA sequence polymorphism in a set of 37 nuclear loci in wild samples of the flat oyster Ostrea edulis. Silent diversity was found to be only moderate (0.7%), and there was no departure from demographic equilibrium under the...
Tipo: Text
Palavras-chave: Nucleotide; Polymorphism; Marine bivalve; Deleterious; Mutations; Genetic load; Ostrea edulis.
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00125/23610/21444.pdf

Contribution of in Vivo Experimental Challenges to Understanding Flat Oyster Ostrea edulis Resistance to Bonamia ostreae
Provedor de dados: 5
Autores: Morga, Benjamin; Renault, Tristan; Faury, Nicole; Lerond, Sophie; Garcia, Celine; Chollet, Bruno; Joly, Jean-pierre; Lapegue, Sylvie; Harrang, Estelle; Arzul, Isabelle.
Bonamiosis due to the parasite Bonamia ostreae has been associated with massive mortality outbreaks in European flat oyster stocks in Europe. As eradication and treatment are not possible, the control of the disease mainly relies on transfer restriction. Moreover, selection has been applied to produce resistant flat oyster families, which present better survival and lower prevalence than non-selected oysters. In order to better understand the mechanisms involved in resistance to bonamiosis, cellular and molecular responses of 2 oyster groups (selected oysters and wild-type oysters) were analyzed in the context of experimental injection and cohabitation infections. Cellular responses including non-specific esterases detection, ROS production and...
Tipo: Text
Palavras-chave: Bonamia ostreae; Protozoan; Ostrea edulis; Resistance; Experimental infection; Haemocytes; Gene expression; Apoptosis.
Ano: 2017
URL: http://archimer.ifremer.fr/doc/00405/51665/52213.pdf

Development of SNP genotyping arrays in two shellfish species
Provedor de dados: 5
Autores: Lapegue, Sylvie; Harrang, Estelle; Heurtebise, Serge; Flahauw, Emilie; Donnadieu, C.; Gayral, P.; Ballenghien, M.; Genestout, L.; Barbotte, L.; Mahla, R.; Haffray, P.; Klopp, C.
Use of SNPs has been favored due to their abundance in plant and animal genomes, accompanied by the falling cost and rising throughput capacity for detection and genotyping. Here, we present in vitro (obtained from targeted sequencing) and in silico discovery of SNPs, and the design of medium-throughput genotyping arrays for two oyster species, the Pacific oyster, Crassostrea gigas, and European flat oyster, Ostrea edulis. Two sets of 384 SNP markers were designed for two Illumina GoldenGate arrays and genotyped on more than 1000 samples for each species. In each case, oyster samples were obtained from wild and selected populations and from three-generation families segregating for traits of interest in aquaculture. The rate of successfully genotyped...
Tipo: Text
Palavras-chave: Crassostrea gigas; GoldenGate technology; Ostrea edulis; Oysters; SNP genotyping.
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00172/28369/26677.pdf

Apport des informations moléculaires et cellulaires pour la caractérisation de la résistance de l'huître plate européenne vis-à-vis de la bonamiose, et pour la détection de signatures de la sélection naturelle
Provedor de dados: 5
Autores: Harrang, Estelle.
The European flat oyster, an endemic species from European coasts, has been classified in the category of "endangered and/or declining species" since 2003. Indeed, the natural beds of this oyster, consumed since ancient times, have gradually been decimated by over-exploitation and by successive emergence of parasitic diseases. The parasite that causes the disease called bonamiosis has contributed to drastically reduce the French and European aquacultural production of flat oyster. Marine bivalve molluscs display two specificities that restrict possibilities to fight against diseases: they are grown in an open environment, and possess an innate immune system lacking in adaptive response. In this context, the selection of animaIs naturally resistant to...
Tipo: Text
Palavras-chave: Huître plate Européenne; Ostre edulis; Bonamiose; Bonamia ostreae; Marqueurs SNP; Expression génique; Activité hémocytaire; Cartographie de liaison; QTL; EQTL; Populations naturelles; Diversité génétique; Sélection naturelle; European flat oyster; Ostrea edulis; Bonamiosis; Bonamia ostreae; SNP markers; Gene expression; Haemocytic activity; Linkage map; QTL; EQTL; Natural populations; Genetic diversity; Genetic structure; Natural selection.
Ano: 2012
URL: http://archimer.ifremer.fr/doc/00127/23785/21702.pdf

The use of SNPs in characterizing oysters genomes and their resistance to pathogens
Provedor de dados: 5
Autores: Lapegue, Sylvie; Heurtebise, Serge; Harrang, Estelle; Morga, Benjamin; Flahauw, Emilie; Sauvage, Christopher; Boudry, Pierre.
During the last five years, a great effort has been made to develop genomic tools in oyster species all over the world. In Europe, the Pacific cupped oyster, Crassostrea gigas, and the European flat oyster, Ostrea edulis received a special attention as they are the key species for local aquaculture. In particular, the Pacific oyster is a marine bivalve of major economical and ecological importance worldwide. Better knowledge of its physiology and its genetics contributes to optimize its aquaculture production using selective breeding or to understand its invasive behavior in some coastal areas. Current international developments of genomic resources include EST databases, BAC libraries, genome mapping and sequencing. The Genoscope (Evry, France) and the...
Tipo: Text
Palavras-chave: Oysters; Crassostrea gigas; Ostrea edulis; Resistance pathogens; QTLs; Single Nucleotide Polymorphism.
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00015/12584/9454.pdf