Consulta: creatorFacets:"Hervio-heath, Dominique"
Registros recuperados: 17
Data/hora: 01/06/2024 11:47:24

High genetic diversity of     Vibrio cholerae     in the European lake Neusiedler See is associated with intensive recombination in the reed habitat and the long-distance transfer of strains
Provedor de dados: 5
Autores: Pretzer, Carina; Druzhinina, Irina S.; Amaro, Carmen; Benediktsdottir, Eva; Hedenstroem, Ingela; Hervio-heath, Dominique; Huhulescu, Steliana; Schets, Franciska M.; Farnleitner, Andreas H.; Kirschner, Alexander K. T.
Coastal marine Vibrio cholerae populations usually exhibit high genetic diversity. To assess the genetic diversity of abundant V. cholerae non-O1/non-O139 populations in the Central European lake Neusiedler See, we performed a phylogenetic analysis based on recA, toxR, gyrB and pyrH loci sequenced for 472 strains. The strains were isolated from three ecologically different habitats in a lake that is a hot-spot of migrating birds and an important bathing water. We also analyzed 76 environmental and human V. cholerae non-O1/non-O139 isolates from Austria and other European countries and added sequences of seven genome-sequenced strains. Phylogenetic analysis showed that the lake supports a unique endemic diversity of V. cholerae that is particularly rich in...
Tipo: Text
Ano: 2017
URL: http://archimer.ifremer.fr/doc/00366/47756/47802.pdf

Chlorophyll a might structure a community of potentially pathogenic culturable Vibrionaceae. Insights from a one-year study of water and mussels surveyed on the French Atlantic coast
Provedor de dados: 5
Autores: Deter, Julie; Lozach, Solen; Derrien, Annick; Veron, Antoine; Chollet, Jaufrey; Hervio-heath, Dominique.
The present study focused on the isolation of culturable bacteria from mussels and sea water to identify Vibrionaceae potentially pathogenic for humans. Three sites located on the French Atlantic coast were monitored monthly (twice each month during summer) for 1 year. Environmental parameters were surveyed (water temperature, salinity, turbidity, chlorophyll a) and bacteria were detected by culture and identified by API 20E(R) systems (BioMerieux) and PCR. A total of seven species were detected (Grimontia hollisae, Photobacterium damselae, Vibrio alginolyticus, V. cholerae, V. fluvialis, V. vulnificus and V. parahaemolyticus) and species diversity was higher at the end of summer. Surprisingly, V. cholerae non-O1/non-O139 was detected in spring. No site...
Tipo: Text
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00008/11928/8636.pdf

Diversity of Vibrio spp. isolated at ambient environmental temperature in the Eastern English Channel as determined by pyrH sequencing
Provedor de dados: 5
Autores: Tall, Amadou; Hervio-heath, Dominique; Teillon, Anna; Boisset-helbert, C.; Delesmont, R.; Bodilis, J.; Touron-bodilis, A.
Aims To describe the diversity of the culturable mesophilic and potentially pathogenic vibrios isolated at 22 and 37 degrees C on TCBS medium, in September 2009 from seawater and surface sediments. Methods and Results q-PCR assays previously selected for the identification of bacterial strains isolated at 37 degrees C were used in combination with the partial sequencing of two housekeeping genes, pyrH and toxR, to identify 315 strains isolated at 22 degrees C. The great majority of the 37 degrees C strains was identified by q-PCR assays, (five of the six species) with the predominance of Vibrio alginolyticus (85 center dot 9%) and V.harveyi (10 center dot 7%). The human pathogens V.parahaemolyticus and V.cholerae were rarely detected (two strains each)....
Tipo: Text
Palavras-chave: Coastal environment; Culturable Vibrio spp; Diversity; PyrH and toxR genes partial sequencing.
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00144/25502/24832.pdf

Quantification of Vibrio parahaemolyticus, Vibrio vulnificus and Vibrio cholerae in French Mediterranean coastal lagoons
Provedor de dados: 5
Autores: Cantet, Franck; Hervio-heath, Dominique; Caro, A; Le Mennec, Cecile; Monteil, Caroline; Quemere, Catherine; Jolivet-gougeon, Anne; Colwell, Rita R.; Monfort, Patrick.
Vibrio parahaemolyticus, Vibrio vulnificus and Vibrio cholerae are human pathogens. Little is known about these Vibrio spp. in the coastal lagoons of France. The purpose of this study was to investigate their incidence in water, shellfish and sediment of three French Mediterranean coastal lagoons using the most probable number-polymerase chain reaction (MPN-PCR). In summer, the total number of V. parahaemolyticus in water, sediment, mussels and clams collected from the three lagoons varied from 1 to &gt;1.1 x 10.3 MPN/l, 0.09 to 1.1 x 10.3 MPN/ml, 9 to 210 MPN/g and 1.5 to 2.1 MPN/g, respectively. In winter, all samples except mussels contained V. parahaemolyticus, but at very low concentrations. Pathogenic (tdh- or trh2-positive) V. parahaemolyticus were...
Tipo: Text
Palavras-chave: Vibrio; Lagoons; Shellfish; Water; Sediment; Human pathogen.
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00148/25890/23889.pdf

Qualité microbiologique des coquillages destinés à la consommation humaine : contamination des coquillages par des bactéries pathogènes pour l’homme. Sous-région marine Golfe de Gascogne. Evaluation initiale DCSMM.
Provedor de dados: 5
Autores: Hervio-heath, Dominique; Gourmelon, Michele; Catherine, Martial.
Tipo: Text
Palavras-chave: Golfe de Gascogne; Coquillages; Contamination microbiologique; Bactéries; Pathogènes; Suivi.
Ano: 2012
URL: https://archimer.ifremer.fr/doc/00329/44061/43632.pdf

Effect of oxygen and temperature on the dynamic of the dominant bacterial populations of pig manure and on the persistence of pig-associated genetic markers, assessed in river water microcosms
Provedor de dados: 5
Autores: Marti, R.; Mieszkin, Sophie; Solecki, O.; Pourcher, A. -m.; Hervio-heath, Dominique; Gourmelon, Michele.
Aims: The aim is to evaluate the dynamic of Bacteroides-Prevotella and Bacillus-Streptococcus-Lactobacillus populations originating from pig manure and the persistence of pig-associated markers belonging to these groups according to temperature and oxygen. Methods and Results: River water was inoculated with pig manure and incubated under microaerophilic and aerobic conditions, at 4 and 20 degrees C over 43 days. The diversity of bacterial populations was analysed by capillary electrophoresis-single-strand conformation polymorphism. The persistence of the pig-associated markers was measured by real-time PCR and compared with the survival of Escherichia coli and enterococci. Decay was characterized by the estimation of the time needed to produce a 1-log...
Tipo: Text
Palavras-chave: Lactobacillus amylovorus; Microbial source tracking; Oxygen; Persistence; Pig-associated Bacteroidales markers; Quantitative real-time PCR; River water; Temperature.
Ano: 2011
URL: http://archimer.ifremer.fr/doc/00053/16470/14534.pdf

Contamination des coquillages par des bactéries pathogènes pour l’homme. Sous-région marine Méditerranée occidentale. Evaluation initiale DCSMM.
Provedor de dados: 5
Autores: Hervio-heath, Dominique; Gourmelon, Michele; Catherine, Martial.
Tipo: Text
Palavras-chave: Méditerranée; Coquillages; Contamination; Suivi; Bactéries pathogènes.
Ano: 2012
URL: https://archimer.ifremer.fr/doc/00331/44244/43813.pdf

Occurrence of Bacterial Pathogens and Human Noroviruses in Shellfish-Harvesting Areas and Their Catchments in France
Provedor de dados: 5
Autores: Rince, Alain; Baliere, Charlotte; Hervio-heath, Dominique; Cozien, Joelle; Lozach, Solen; Parnaudeau, Sylvain; Le Guyader, Francoise S.; Le Hello, Simon; Giard, Jean-christophe; Sauvageot, Nicolas; Benachour, Abdellah; Strubbia, Sofia; Gourmelon, Michele.
During a 2-year study, the presence of human pathogenic bacteria and noroviruses was investigated in shellfish, seawater and/or surface sediments collected from three French coastal shellfish-harvesting areas as well as in freshwaters from the corresponding upstream catchments. Bacteria isolated from these samples were further analyzed. Escherichia coli isolates classified into the phylogenetic groups B2, or D and enterococci from Enterococcus faecalis and E. faecium species were tested for the presence of virulence genes and for antimicrobial susceptibility. Salmonella members were serotyped and the most abundant serovars (Typhimurium and its monophasic variants and Mbandaka) were genetically characterized by high discriminative subtyping methods....
Tipo: Text
Palavras-chave: Campylobacter; Salmonella; Vibrio; HuNoVs; Fecal bacterial indicators; Shellfish; Water.
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00461/57243/59243.pdf

Qualité microbiologique des coquillages destinés à la consommation humaine : contamination des coquillages par des bactéries pathogènes pour l’homme. Sous-région marine Mers celtiques. Evaluation initiale DCSMM.
Provedor de dados: 5
Autores: Hervio-heath, Dominique; Gourmelon, Michele; Catherine, Martial.
Tipo: Text
Palavras-chave: Mers celtiques; Coquillages; Contamination microbiologique; Bactéries pathogènes.
Ano: 2012
URL: https://archimer.ifremer.fr/doc/00329/43977/43549.pdf

Historical records from dated sediment cores reveal the multidecadal dynamic of the toxic dinoflagellate     Alexandrium minutum     in the Bay of Brest (France)
Provedor de dados: 5
Autores: Klouch, Khadidja; Schmidt, Sabine; Andrieux-loyer, Francoise; Le Gac, Mickael; Hervio-heath, Dominique; Qui-minet, Zujaila Nohemi; Quere, Julien; Bigeard, Estelle; Guillou, Laure; Siano, Raffaele.
The multiannual dynamic of the cyst-forming and toxic marine dinoflagellate Alexandrium minutum was studied over a time scale of about 150 years by a paleoecological approach based on ancient DNA (aDNA) quantification and cyst revivification data obtained from two dated sediment cores of the Bay of Brest (Brittany, France). The first genetic traces of the species presence in the study area dated back to 1873 ± 6. Specific aDNA could be quantified by a newly-developed real-time PCR assay in the upper core layers, in which the germination of the species (in up to 17–19 year-old sediments) was also obtained. In both cores studied, our quantitative paleogenetic data showed a statistically significant increasing trend in the abundance of A. minutum ITS1 rDNA...
Tipo: Text
Palavras-chave: Paleoecology; Ancient DNA; Real-time PCR; Harmful algal blooms (HAB); Dinoflagellates; Coastal ecology.
Ano: 2016
URL: http://archimer.ifremer.fr/doc/00334/44516/44212.pdf

Epidemiological investigation of a foodborne outbreak in Spain associated with U.S. West Coast genotypes of Vibrio parahaemolyticus
Provedor de dados: 5
Autores: Martinez-urtaza, Jaime; Powell, Andy; Jansa, Josep; Castro Rey, Jose Luis; Paz Montero, Oscar; Garcia Campello, Marta; Zamora Lopez, Ma Jose; Pousa, Anxela; Faraldo Valles, Ma Jose; Trinanes, Joaquin; Hervio-heath, Dominique; Keay, William; Bayley, Amanda; Hartnell, Rachel; Baker-austin, Craig.
We describe an outbreak of seafood-associated Vibrio parahaemolyticus in Galicia, Spain in on 18th of August 2012 affecting 100 of the 114 passengers travelling on a food banquet cruise boat. Epidemiological information from 65 people was available from follow-on interviews, of which 51 cases showed symptoms of illness. The food items identified through the questionnaires as the most probable source of the infections was shrimp. This product was unique in showing a statistically significant and the highest OR with a value of 7.59 (1.52–37.71). All the nine strains isolated from stool samples were identified as V. parahaemolyticus, seven were positive for both virulence markers tdh and trh, a single strain was positive for trh only and the remaining strain...
Tipo: Text
Palavras-chave: Seafood; Tdh; Trh; Vibrio parahaemolyticus; PNW clone; ST 36.
Ano: 2016
URL: http://archimer.ifremer.fr/doc/00311/42181/41482.pdf

Rapid proliferation of Vibrio parahaemolyticus, Vibrio vulnificus and Vibrio cholerae during freshwater flash floods in French Mediterranean coastal lagoons
Provedor de dados: 5
Autores: Esteves, Kevin; Hervio-heath, Dominique; Mosser, Thomas; Rodier, Claire; Tournoud, Marie-george; Jumas-bilak, Estelle; Colwell, Rita R.; Monfort, Patrick.
V. parahaemolyticus, V. vulnificus and V. cholerae non-O1/non-O139 are present in coastal lagoons of Southern France. In these Mediterranean regions, the rivers have long low flow periods followed by short-duration or flash floods during and after heavy intense rainstorms, particularly at the end of the summer and in autumn. These floods bring large volumes of freshwater into the lagoons, reducing their salinity. Water temperatures recorded during sampling (15°C to 24°C) were favorable for presence and multiplication of vibrios. In autumn 2011, before heavy rainfalls and flash floods, salinities ranged from 31.4‰ to 36.1‰ and concentrations of V. parahaemolyticus, V. vulnificus, and V. cholerae varied from 0 to 1.5 X 103 MPN (Most Probable Number)/L, 0.7...
Tipo: Text
Ano: 2015
URL: http://archimer.ifremer.fr/doc/00279/39038/37584.pdf

A pan-European ring trial to validate an International Standard for detection of Vibrio cholerae , Vibrio parahaemolyticus and Vibrio vulnificus in seafoods
Provedor de dados: 5
Autores: Hartnell, R. E.; Stockley, L.; Keay, W.; Rosec, J. -p.; Hervio-heath, Dominique; Van Den Berg, H.; Leoni, F.; Ottaviani, D.; Henigman, U.; Denayer, S.; Serbruyns, B.; Georgsson, F.; Krumova-valcheva, G.; Gyurova, E.; Blanco, C.; Copin, S.; Strauch, E.; Wieczorek, K.; Lopatek, M.; Britova, A.; Hardouin, G.; Lombard, B.; Veld, P. In'T; Leclercq, A.; Baker-austin, C.
Globally, vibrios represent an important and well-established group of bacterial foodborne pathogens. The European Commission (EC) mandated the Comite de European Normalisation (CEN) to undertake work to provide validation data for 15 methods in microbiology to support EC legislation. As part of this mandated work programme, merging of ISO/TS 21872–1:2007, which specifies a horizontal method for the detection of V. parahaemolyticus and V. cholerae, and ISO/TS 21872–2:2007, a similar horizontal method for the detection of potentially pathogenic vibrios other than V. cholerae and V. parahaemolyticus) was proposed. Both parts of ISO/TS 21872 utilized classical culture-based isolation techniques coupled with biochemical confirmation steps. The work also...
Tipo: Text
Palavras-chave: Vibrios; Seafood; Prawns; Oysters; Biochemical methods; Real-time PCR; Conventional PCR.
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00425/53680/54525.pdf

Passive Samplers, a Powerful Tool to Detect Viruses and Bacteria in Marine Coastal Areas
Provedor de dados: 5
Autores: Vincent Hubert, Francoise; Wacrenier, Candice; Morga, Benjamin; Lozach, Solen; Quenot, Emmanuelle; Mege, Mickael; Lecadet, Cyrielle; Gourmelon, Michele; Hervio-heath, Dominique; Le Guyader, Soizick.
The detection of viruses and bacteria which can pose a threat either to shellfish health or shellfish consumers remains difficult. The current detection methods rely on point sampling of water, a method that gives a snapshot of the microorganisms present at the time of sampling. In order to obtain better representativeness of the presence of these microorganisms over time, we have developed passive sampling using the adsorption capacities of polymer membranes. Our objectives here were to assess the feasibility of this methodology for field detection. Different types of membrane were deployed in coastal waters over 2 years and the microorganisms tested using qPCR were: human norovirus (NoV) genogroups (G)I and II, sapovirus, Vibrio spp. and the species...
Tipo: Text
Palavras-chave: Norovirus; Ostreid herpes virus 1 &amp;#956; Var; Vibrio spp; Microbial source tracking; Sea; Passive sampler; Oyster (Crassostrea gigas).
Ano: 2021
URL: https://archimer.ifremer.fr/doc/00682/79375/81893.pdf

Real-Time PCR optimization to identify environmental Vibrio spp. strains
Provedor de dados: 5
Autores: Tall, Amadou; Teillon, Anna; Boisset, Claire; Delesmont, R.; Touron-bodilis, A.; Hervio-heath, Dominique.
Aims: To identify Vibrio vulnificus, Vibrio cholerae and Vibrio alginolyticus using standardized DNA extraction method and real-time PCR assays, among a large number of bacterial strains isolated from marine environment. Methods and Results: Methods for DNA extraction and real-time PCR were standardized to identify a large number of Vibrio spp. strains isolated through regular collection campaigns of environmental samples. Three real-time PCR assays were developed from a multiplex PCR, targeting V. vulnificus, V. cholerae and V. alginolyticus on the dnaJ gene. After testing their specificity, these systems were applied for the identification of 961 strains isolated at 22°C (446 strains) and 37°C (515 strains) in September 2009. The predominance of V....
Tipo: Text
Palavras-chave: DnaJ; Identification; Real-time PCR; Vibrio alginolyticus; Vibrio cholerae; Vibrio vulnificus.
Ano: 2012
URL: http://archimer.ifremer.fr/doc/00088/19901/17562.pdf

Highly diverse recombining populations of Vibrio cholerae and Vibrio parahaemolyticus in French Mediterranean coastal lagoons
Provedor de dados: 5
Autores: Esteves, Kevin; Mosser, Thomas; Aujoulat, Fabien; Hervio-heath, Dominique; Monfort, Patrick; Jumas-bilak, Estelle.
Vibrio parahaemolyticus and Vibrio cholerae are ubiquitous to estuarine and marine environments. Thes two species found in Mediterranean coastal systems can induce infection in humans. Environmental isolates of V. cholerae (n = 109) and V. parahaemotycius (n = 89) sampled at different dates, stations and water salinities were investigated for virulence genes and by a mutilocus sequence-based analysis (MLSA). V. cholerae isolate were all ctxA negative and only one isolate of V. parahaemolyticus displayed trh2 gene. Most Sequence Types (ST) corresponded to uique ST isolated at one date or one station. Frequent recombination events were detected among different pathogenic species, V. parahaemotyticus, V. cholerae, Vibrio mimicus, and Vibrio metoecus....
Tipo: Text
Palavras-chave: Vibrio; Human pathogens; French coastal lagoons; Phylogeny; Recombination; Multi-locus sequence analysis; Virulence factor.
Ano: 2015
URL: http://archimer.ifremer.fr/doc/00274/38528/37039.pdf

Qualité microbiologique des coquillages destinés à la consommation humaine : contamination des coquillages par des bactéries pathogènes pour l’homme. Sous-région marine Manche - Mer du Nord. Evaluation initiale DCSMM.
Provedor de dados: 5
Autores: Hervio-heath, Dominique; Gourmelon, Michele; Catherine, Martial.
Tipo: Text
Palavras-chave: Manche; Mer du Nord; Coquillages; Contamination microbiologique; Bactéries pathogènes; Suivi.
Ano: 2012
URL: https://archimer.ifremer.fr/doc/00328/43874/43448.pdf