Consulta: creatorFacets:"Heurtebise, Serge"
Registros recuperados: 79
Data/hora: 01/06/2024 07:09:47

A preliminary genetic linkage map for the European flat oyster Ostrea edulis L.
Provedor de dados: 5
Autores: Lallias, Delphine; Lapegue, Sylvie; Harley, Crhis; Boudry, Pierre; Heurtebise, Serge; Beaumont, Andy.
The flat oyster Ostrea edulis is the species endemic to European coasts, both Atlantic and Mediterranean. It has been exploited since Roman times, but its aquaculture production decreased from around 20000 tons in the 1950's to 1500 tons today because of two successive diseases due to the intracellular parasites Marteilia refringens and Bonamia ostreae. Since 1985, Ifremer has been undertaking a breeding program to produce families of oysters tolerant to Bonamia. In this context, a further step would be to identify QTLs for resistance to this parasite. Therefore, as a first step a genetic map is now being built. The establishment of a genetic linkage map will provide a foundation for the mapping of QTLs, with the ultimate objective to implement...
Tipo: Text
Palavras-chave: QTLs; Ostrea edulis; Flat oyster; Genetic linkage map; Genetic.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/acte-3466.pdf

A first genetic linkage map of the European flat oyster, Ostrea edulis : towards the identification of Bonamia resistance QTLs
Provedor de dados: 5
Autores: Lallias, Delphine; Lapegue, Sylvie; Haley, Chris; Heurtebise, Serge; Boudry, Pierre; Beaumont, Andy.
The flat oyster Ostrea edulis is an endemic European oyster species, found on both Atlantic and Mediterranean coasts. Its aquacultural production has dramatically decreased due to two successive diseases caused by the intracellular parasites Marteilia refringens and Bonamia ostreae. Since 1985, Ifremer has undertaken a breeding program to produce oyster families which are tolerant to Bonamia. In this context, the next step is to identify QTLs for resistance to this parasite. A genetic map is therefore being established as a basis for QTL work. The reference family is an F2 family produced by single pair mating of two oysters from the same F1 family. The F1 family was itself produced from a bi-parental cross between a wild oyster and an individual from the...
Tipo: Text
Palavras-chave: Genetic linkage map; Parasite; Genetic; QTLs; Resistance; Bonamia ostreae; Ostrea edulis; Flat oyster.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/acte-3464.pdf

Fragmentation des populations naturelles d'Ostrea edulis: une adaptation locale de l'huître plate européenne
Provedor de dados: 5
Autores: Harrang, Estelle; Bierne, Nicolas; Heurtebise, Serge; Morga, Benjamin; Lapegue, Sylvie.
L'huître plate européenne Ostrea edulis a connu une période faste dans l'aquaculture jusqu'à la fin des années 1960, lorsque deux parasites, Marteilia refringens (1969) et Bonamia ostreae (1979), ont successivement décimé les bancs naturels de cette espèce endémique, déjà affaiblis par la sur-exploitation de ses gisements. Historiquement présente sur toute la côte européenne, de la Mer du Nord à la Mer Méditerranée, en passant par l'Océan Atlantique, la Mer Adriatique et la Mer Noire, les bancs naturels de cette espèce sont devenus très localisés, laissant penser à des phénomènes d'adaptation locale de l'huître vis à vis de son environnement biotique et abiotique.  La diversité et la structuration génétique de plusieurs populations de l'espèce, à l'échelle...
Tipo: Text
Palavras-chave: Huître plate; Ostrea edulis; Génétique des populations; Sélection Génétique.
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00014/12547/9417.pdf

Bilan des productions expérimentales d'huîtres plates de 2005 à 2010 au Laboratoire de Génétique et Pathologie de La Tremblade
Provedor de dados: 5
Autores: Heurtebise, Serge; Harrang, Estelle; Lallias, Delphine; Lapegue, Sylvie.
L’objectif de ce rapport est de détailler les productions d’huîtres plates européennes, Ostrea edulis, dans le cadre des projets de recherche développés autour de l’amélioration génétique de cette espèce. Nous avons choisi de synthétiser les actions de recherche dans un premier temps puis la production des huîtres et les expérimentations dans un ordre chronologique.
Tipo: Text
Palavras-chave: Huître plate; Ostrea edulis; Production expérimentale; Amélioration génétique.
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00013/12421/9211.pdf

Etude de la mortalité des moules dans le Pertuis Breton en 1989
Provedor de dados: 5
Autores: Dardignac, Marie-jose; Bodoy, Alain; Garnier, Jacqueline; Heurtebise, Serge; Legrand, Jacqueline.
Une recherche des causes de la mortalité des moule au printemps dans le Pertuis Breton a été entreprise en 1989. La faible pluviométrie observée cette année là n'a pas entrainé de dessalures importantes et les mortalités constatées ont été si faibles qu'elles n 'ont pas eu de conséquences sur la production de moules. Les essais réalisés en milieu contrôlé ont montré que même pendant la période de reproduction au cours de laquelle les moules sont plus fragiles, il fallait des salinités inférieures à 15 p . mille et se maintenant pendant plusieurs jours avant que des mortalités n 'apparaissent. Il est prévu de remettre le même programme en place au printemps 1990.
Tipo: Text
Palavras-chave: Moules; Bouchots; Mortalités; Pollution; Pertuis Breton.
Ano: 1990
URL: http://archimer.ifremer.fr/doc/00048/15902/13330.pdf

Can survival of European flat oysters following experimental infection with Bonamia ostreae be predicted using QTLs?
Provedor de dados: 5
Autores: Harrang, Estelle; Heurtebise, Serge; Faury, Nicole; Robert, Maeva; Arzul, Isabelle; Lapegue, Sylvie.
The present study identifies quantitative trait loci (QTLs) in response to an experimental infection with the parasite responsible for bonamiosis, Bonamia ostreae, in two segregating families of the European flat oyster, Ostrea edulis. We first constructed a genetic-linkage map for each studied family and improved the existing genetic-linkage map for the European flat oyster with a set of SNP markers. This latter map now combines the best accuracy and the best estimate of the genome coverage available for an oyster species. Secondly, by comparing the QTLs detected in this study with those previously published for O. edulis in similar experimental conditions, we identified several potential QTLs that were identical between the different families, and also...
Tipo: Text
Palavras-chave: Ostrea edulis; Single Nucleotide Polymorphism; Disease resistance; Genetic and QTL mapping; Candidate genes.
Ano: 2015
URL: http://archimer.ifremer.fr/doc/00271/38244/36429.pdf

Obtention de souches conchylicoles performantes par polyploïdisation (3ème partie)
Provedor de dados: 5
Autores: Gerard, Andre; Peignon, Jean-marie; Ledu, Christophe; Phelipot, Pascal; Noiret, Christophe; Bodoy, Alain; Heurtebise, Serge; Garnier, Jacqueline.
The third part of the polyploidisation program of the main French species of commercial interest, this report presents the actions and results for the year 1991 which was devoted to:- continuation of the development trials of the triploidy induction technique in Ruditapes decussates (i.e. indigenous clam)- production of diploid and triploid populations of Ostrea edulis (i.e. flat oyster)- control of diploid and triploid population performances in cupped oyster and Pacific clam in their natural habitat- continuation of the development of a ploidy control technique through image analysis- beginning of the development of a new polyploidy induction technique in bivalve molluscs
Tipo: Text
Palavras-chave: Ruditapes decussatus; Ostrea edulis; Ruditapes philippinarum; Crassostrea gigas; Imagerie numérique; Cytogénétique; Polyploïdie.
Ano: 1992
URL: http://archimer.ifremer.fr/doc/1992/rapport-3857.PDF

Etude transcriptomique, génétique et fonctionnelle de la gamétogenèse chez l'huître creuse Crassostrea gigas
Provedor de dados: 5
Autores: Bigot, L.; Dubos, M. P.; Dheilly, N.; Favrel, P.; Kellner, K.; Lelong, C.; Martinez, A. S.; Riviere, G.; Santerre, C.; Sourdaine, P.; Degremont, Lionel; Flahauw, Emilie; Hatt, Philippe-jacques; Heurtebise, Serge; Lapegue, Sylvie; Azzouzi, N.; Galibert, F.; Boudry, Pierre; Corporeau, Charlotte; Fabioux, Caroline; Guevelou, Eric; Huvet, Arnaud; Sussarellu, Rossana.
Le projet GAMETOGENES vise à améliorer les connaissances des bases physiologiques et génétiques de la reproduction et des métabolismes associés chez un mollusque d'importance économique l'huître creuse, Crassostrea gigas. Il a pour objectifs (1) la définition de marqueurs clés de la gamétogenèse, de l'investissement reproducteur et du déterminisme du sexe chez une espèce hermaphrodite à protandrie irrégulière (2) la recherche de QTL de survie aux mortalités estivales ou à l'effort de reproduction dont le succès repose sur l'augmentation de la densité de marqueurs sur les cartes génétiques existantes (SNP en particulier) et sur les liens qui pourront être établis entre ces cartes de liaison et une carte physique (3) le développement d'outils pour valider la...
Tipo: Text
Palavras-chave: Génétique; Huître creuse; Crassostrea gigas; Gamétogenèse; QTL; Marqueur; Physiologie.
Ano: 2012
URL: http://archimer.ifremer.fr/doc/00107/21781/19355.pdf

Individual growth variation and its relationship with survival in juvenile Pacific oysters, Crassostrea gigas (Thunberg)
Provedor de dados: 5
Autores: Boudry, Pierre; Collet, B; Mccombie, Helen; Ernande, Bruno; Morand, B; Heurtebise, Serge; Gerard, Andre.
In order to study individual growth variability and its relationship with survival in juvenile Crassostrea gigas, parental oysters were sampled at four sites located along the French Atlantic coast and bred under controlled hatchery conditions. Four groups of larvae were obtained by crossing five males and five females from each of the four sites, and a fifth group by crossing these 20 males and 20 females together in a pool. Larvae were reared under conditions allowing the maintenance of a maximum variability of size and gave five experimental groups. Oysters were individually monitored for growth and survival from 3 to 10 months after fertilization. The individual growth performances were relatively stable over time and no noticeable compensation for...
Tipo: Text
Palavras-chave: Survival; Oysters; Growth; Crassostrea gigas; Aquaculture.
Ano: 2003
URL: http://archimer.ifremer.fr/doc/2003/publication-467.pdf

Performance of triploid Pacific oysters Crassostrea gigas (Thunberg) reared in high carrying capacity ecosystem : survival, growth and proximate biochemical composition
Provedor de dados: 5
Autores: Goulletquer, Philippe; Joly, Jean-pierre; Gerard, Andre; Le Gagneur, Eric; Moriceau, Jacques; Peignon, Jean-marie; Heurtebise, Serge; Phelipot, Pascal.
Triploid oysters Crassllstrea gigas were: produced in 1990 by treating fertilized eggs with cytochalasin B (CS). Triploids, treated diploids, and controls were deployed early 1991 in a high carrying capacity ecosystem on the Eastern Coast of Normandy (France). A monthly monitoring in 1992 showed that triploids yielded significantly higher growth rate and biochemical composition. However, growth was more heterogeneous. No evidence was found for a length or weight bimodal frequency distribution within triploid groups after a 26 months rearing cycle. Triploids showed retardation of gametogenesis. Carbohydrates content in triploids remained almost constant (40%) from June to September. Their survival rates were significantly lower than controls. Moreover...
Tipo: Text
Palavras-chave: Biochemical compostion; Survival; Carrying capacity; Triploid; Growth; Oysters; Crassostrea gigas; Composition biochimique; Survie; Capacité trophque; Triploïdes; Croissance; Huîtres; Crassostrea gigas.
Ano: 1996
URL: http://archimer.ifremer.fr/doc/1996/publication-3088.pdf

Genetic basis of growth, survival and their plasticity in the Pacific oyster Crassostrea gigas
Provedor de dados: 5
Autores: Ernande, Bruno; Boudry, Pierre; Heurtebise, Serge; Haure, Joel; Martin, Jean-louis.
Introduction : Growth and survival are the life-history traits of the most interest for oyster production since they determine biomass output (growth x survival). This study aimed to investigate whether there is genetic basis for growth, survival and their plasticity to know if a selection program is conceivable for these traits. Material and Methods : Nested half-sibs mating design was used to produce families of oysters: 5 males were each mated to three females producing 5 half-sibs families, each containing 3 full-sib families. At 15 months of age (May 1999), each full-sibs family was randomly split into five groups which were given different treatments. Two groups were placed in a constant environment: one with low food resources and another with high...
Tipo: Text
Palavras-chave: Genotyic; Plasticity; Survival; Growth; Genetic; Crassostrea gigas; Pacific oyster.
Ano: 2000
URL: http://archimer.ifremer.fr/doc/2000/acte-3498.pdf

Is fertility of hybrids enough to conclude that the two oysters Crassostrea gigas and Crassostrea angulata are the same species?
Provedor de dados: 5
Autores: Huvet, Arnaud; Gerard, Andre; Ledu, Christophe; Phelipot, Pascal; Heurtebise, Serge; Boudry, Pierre.
The distinction of the two cupped oysters Crassostrea gigas (Thunberg, 1793) and Crassostrea angulata (Lamark, 1819) into two species was chiefly due to their differing geographical distributions, C. gigas being present in Asia and C. angulata in Europe. Today it is commonly accepted that C. angulata and C. gigas are a single species according to morphological, genetic and F1 hybridization data. However, the demonstration of the fertility of their hybrids and the absence of any reproductive isolation remained to be investigated. Consequently, we studied the fertility of hybrids and sperm competition by performing three different experiments and producing G1 and G2 hybrid progenies between wild populations of C. angulata and C. gigas. Progenies showed very...
Tipo: Text
Palavras-chave: Crassostrea angulata; Crassostrea gigas; Developmental yields of larvae; Fertility; Controlled crosses; Hybridization.
Ano: 2002
URL: http://archimer.ifremer.fr/doc/2002/publication-1395.pdf

Mapping QTL for resistance to summer mortality in the Pacific oyster Crassostrea gigas
Provedor de dados: 5
Autores: Sauvage, Christopher; Heurtebise, Serge; De Koning, D.; Haley, Chris; Boudry, Pierre; Lapegue, Sylvie.
Summer mortalities of the Pacific cupped oyster Crassostrea gigas have been described in the literature for decades. One solution to overcome this problem would be to select animals more tolerant to summer mortalities, as this trait was shown to be highly heritable (Degrémont, 2003). Such selective breeding would strongly benefit from the identification of markers associated to the resistance to allow the development of markers assisted selection. Recently, a set of a new kinds of markers (SNPs) have been developed in the Pacific oyster. These markers were identified and characterized in several genes involved in the immune response and energetic pathways (Sauvage, 2007). In this context, the mapping of SNPs, of 16 new EST-SSR based markers and of...
Tipo: Text
Palavras-chave: Mortality; Genetic; Crassostrea gigas; Pacific oyster; Mapping QTL.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/acte-3504.pdf

Obtention de souches conchylicoles performantes par polyploïdisation (5ème partie)
Provedor de dados: 5
Autores: Gerard, Andre; Naciri, Yamama; Peignon, Jean-marie; Ledu, Christophe; Phelipot, Pascal; Baud, Jean-pierre; Nourry, Max; Haure, Joel; Heurtebise, Serge; Garnier, Jacqueline; Martin, Anne-genevieve; Claude, Serge; Langlade, Aime; Joly, Jean-pierre; Goulletquer, Philippe; Ruelle, Francois; Coatanea, Denis; Oheix, Jocelyne; Vercelli, Catherine; Chabirand, Jean-michel; Maurer, Daniele; Borel, Michel; Cardinal, Mireille; Cornet, Josiane; Le Pennec, Marcel; Le Mercier, A.
This fifth part of the polyploidisation program reports the experiments that were conducted in 1993 and that were devoted to : - monitoring growth performance of Ostrea edulis diploids and triploids in La Trinité sur Mer and Palavas - monitoring growth performance of Crassostrea gigas diploids and triploids in Port en Bessin, Marennes Oleron (2 locations), Arcachon and Palavas - comparative study of gametogenesis for C. gigas diploids and triploids - comparative study of C. gigas diploids and triploids using sensorial analysis At the end of fifth year, it was clear that triploids are advantageous although such a statement must be moderated according to locations. The gametogenesis stydy showed that triploidy is associated with partial to complete...
Tipo: Text
Palavras-chave: Crassostrea gigas; Ostrea edulis; Cytogenetics; Polyploidy; Crassostra gigas; Ostrea edulis; Cytogénétique; Polyploïdie.
Ano: 1994
URL: http://archimer.ifremer.fr/doc/1994/rapport-3860.PDF

Bonamia ostreae-induced mortalities in one-year old European flat oysters Ostrea edulis: experimental infection by cohabitation challenge
Provedor de dados: 5
Autores: Lallias, Delphine; Arzul, Isabelle; Heurtebise, Serge; Ferrand, Sylvie; Chollet, Bruno; Robert, Maeva; Beaumon, Andy; Boudry, Pierre; Morga, Benjamin; Lapegue, Sylvie.
Bonamiosis is a parasitic disease (causative agent: Bonamia ostreae) affecting the European flat oyster Ostrea edulis, responsible for a drastic decline in its aquaculture production. Selective breeding programs for resistance to bonamiosis have been undertaken to counter this disease. In the present study, a 6-month cohabitation challenge experiment was performed in order to transmit the disease from wild oysters injected with the parasite to two tested families of oysters (20 and 8-month old at the beginning of the experiment, with different genetic backgrounds) originating from a selective breeding program developed by IFREMER in France. Mortalities were checked daily and ventricular heart smears were performed on dying or moribund oysters to detect the...
Tipo: Text
Palavras-chave: Heart smear; Transmission; Cohabitation experiment; Bonamia ostreae; Ostrea edulis.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/publication-4783.pdf

Suivi de la reproduction des mollusques d'élevage 1988
Provedor de dados: 5
Autores: Heurtebise, Serge; Ratiskol, Jacqueline.
L'année 1988 n'a pas offert de conditions hydrobiologiques et climatiques favorables à une reproduction normale des huîtres en élevage dans le bassin de Marennes-Oléron. L'hiver doux et particulièrement pluvieux a apparemment modifié le cycle classique de la gamétogénése et par suite le captage des huîtres dans son ensemble. Le présent rapport est le constat de ce phénomène.
Tipo: Text
Palavras-chave: Reproduction; Captage; Naissain; Crassostrea gigas; Mytilus edulis; Marennes Oléron.
Ano: 1989
URL: http://archimer.ifremer.fr/doc/00072/18286/15858.pdf

Is the Portuguese oyster Crassostrea angulata in Southern Europe endangered by the expansion of the Pacific oyster C. gigas?
Provedor de dados: 5
Autores: Batista, Frederico; Boudry, Pierre; Lapegue, Sylvie; Heurtebise, Serge; Monteiro, C. C.
The Portuguese oyster, Crassostrea angulata, was introduced from Portugal to the French Atlantic Coast in the 1860s. C. angulata quickly settled and expanded and leaded to the development of a new aquacultural industry in France. In the late 1960s, mortality associated with the detection of an iridovirus, led to the wipe out of C. angulata from French Atlantic waters and to the massive introduction of C. gigas to sustain production. In Southern Europe, similar symptoms were also observed in natural stocks of C. angulata from Sado River (Portugal) and from the area of Cadiz (Spain). Nowadays, only very few «pure» populations of C. angulata remain in southern Europe. These populations are potentially endangered by the current expansion of C. gigas...
Tipo: Text
Palavras-chave: Selection; Hybridization; Settlement pattern; Population genetic; Genetic; Oysters; Crassostrea angulata; Crassostrea gigas.
Ano: 2005
URL: http://archimer.ifremer.fr/doc/2005/acte-3310.pdf

Facteurs contrôlant la croissance de Ruditapes philippinarum dans 4 nurseries de production en Charente-Maritime (France)
Provedor de dados: 5
Autores: Heral, Maurice; Deslous-paoli, Jean-marc; Garnier, Jacqueline; Prioul, Daniel; Heurtebise, Serge; Razet, Daniel.
Different types of pregrowth of the Japanese palourde are compared on the some site. The method by upwelling which is the more common used, to have good growth, requires a compromise between the running and the raised biomass.  Two periods are critical, in the summer the growth of mollusks is stopped because of a decrease in the phytoplanktonic production ;  in the winter, a fall in temperature lessens filtration and stops growth. The results concern clams consummatation rates of particular food (chlorophyll, protids, lipids, glucids) as weIl as the way to supply the decreasing growth : increase of phytoplankyonic production in the summer by recycling waters from nurseries that are loaded with ammoniac and raise winter temperatures by using under-ground...
Tipo: Text
Palavras-chave: Nursery; Growth; Ruditapes philippinarum; Clams; Nurserie; Croissance; Ruditapes philippinarum; Palourdes.
Ano: 1982
URL: http://archimer.ifremer.fr/doc/1982/acte-2951.pdf

Acclimatation de nouvelles espèces d'huîtres creuses du genre Crassostrea: hybridation et conservatoire de souches. Contrat Région Poitou-Charentes 1994-1998 - 1ère partie : rapport année 1994
Provedor de dados: 5
Autores: Boudry, Pierre; Naciri, Yamama; Launey, Sophie; Ledu, Christophe; Phelipot, Pascal; Heurtebise, Serge; Gerard, Andre.
Le laboratoire IFREMER "Génétique Aquaculture et Pathologie" (GAP) (La Tremblade et Bouin) a entrepris des actions de recherche sur ces différents points. L'Unité de Recherche en Génétique, en collaboration avec tous Ies membres du Réseau Génétique Mollusques (REGEMO) a axé une partie de ses travaux sur l'acclimatation et l'hybridation de différentes espèces du genre Crassostrea. Ces recherches ont débuté avec le soutien du Conseil Général de Charente-Maritime depuis 1992 sur des essais d'acclimatation de C. virginica, l'huître creuse américaine. En prolongement de cette étude, l'URGE a proposé à la région Poitou Charentes un programme de recherche plus vaste sur l'ensemble des espèces d'huîtres creuses. Le contrat de plan Etat-Région a été signé en...
Tipo: Text
Palavras-chave: Génétique; Acclimatation; Hybridation; Crassostrea; Saccostrea; Génétique population.
Ano: 1994
URL: http://archimer.ifremer.fr/doc/00044/15517/12904.pdf

A first linkage map for the European flat oyster, Ostrea edulis towards the identification of Bonamia resistance QTLs
Provedor de dados: 5
Autores: Lallias, Delphine; Beaumont, Andy; Haley, Chris; Heurtebise, Serge; Boudry, Pierre; Lapegue, Sylvie.
The flat oyster Ostrea edulis is an endemic European oyster species, found on both Atlantic and Mediterranean coasts. Its aquacultural production has dramatically decreased due to two successive diseases caused by the intracellular parasites Marteilia refringens and Bonamia ostreae. Since 1985, Ifremer has undertaken a breeding program to produce oyster families which are tolerant to Bonamia. In this context, a genetic map was therefore established as a basis for Quantitative Trait Loci (QTLs) analysis. The reference family was an F2 family coming from a first bi-parental cross between a wild oyster and an individual from the fifth generation inbred line of the Bonamia tolerance selection program. This inbred line had shown no mortality when reared in...
Tipo: Text
Palavras-chave: Parasite; Bonamia ostreae; Bonamiosis; QTL; Genetic; Ostrea edulis; Flat oyster.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/acte-3459.pdf

Acclimatation de nouvelles espèces d'huîtres creuse du genre Crassostrea: hybridation et conservatoire de souches - Contrat de Plan Etat Région Poitou-Charentes 1994-1998 - Synthèse finale
Provedor de dados: 5
Autores: Boudry, Pierre; Heurtebise, Serge; Huvet, Arnaud; Chollet, Bruno; Ledu, Christophe; Phelipot, Pascal; Gerard, Andre.
Actions menées de 1994 à 1998 au sein du conservatoire de souches : 1. Résultats des importations, acclimatation et hybridation. a) Rappel des conditions d'introduction de nouvelles souches. L'importation de toute espèce est conditionnée par les résultats d'une enquête préliminaire dont l'objet est de rechercher les exigences écologiques de l'espèce et la situation épidémiologique dans son milieu d'origine. Le travail du LGP et de ses partenaires a été partagé en différentes actions: 1. Recherche de contacts dans divers pays étrangers, en vue de l'importation d'huîtres présentant des caractéristiques intéressantes. 2. Importation : L'importation se fait selon les normes du CIEM (Conseil International pour l'Exploration de la Mer) : contrôles pathologiques...
Tipo: Text
Palavras-chave: Génétique; Acclimatation; Hybridation; Marqueurs moléculaire; Marqueurs populations; Crassostrea.
Ano: 1998
URL: http://archimer.ifremer.fr/doc/00044/15520/12907.pdf

Acclimatation de nouvelles espèces d'huîtres creuses du genre Crassostrea: hybridation et conservatoire de souches. Amélioration de la qualité chez l'huître creuse Crassostrea gigas par sélection de souches performantes - Rapport année 1996
Provedor de dados: 5
Autores: Boudry, Pierre; Naciri, Yamama; Collet, Bertrand; Cornette, Florence; Heurtebise, Serge; Ledu, Christophe; Chollet, Bruno; Phelipot, Pascal; Gerard, Andre.
Les objectifs pour l'année 1996 étaient les suivants: Poursuivre les premières reproductions des espèces pour lesquelles nous disposons de géniteurs GO. L'objectif est toujours de limiter le stockage d'animaux importés en produisant une descendance simultanément à des témoins C. gigas qui serviront de comparaison. Tenter des hybridations inter-spécifiques des différents taxons présents au sein du conservatoire en fonction des connaissances disponibles dans la littérature sur ce sujet (voir pour revue Gaffney et Allen, 1993). • Importer de nouvelles souches ou espèces. Élargir la gamme des espèces présentes au sein du conservatoire de souches (les capacités d'accueil de la salle de quarantaine étant Limitée).
Tipo: Text
Palavras-chave: Génétique; Acclimatation; Hybridation; Crassostrea; Sélection génétique; Saccostrea commercialis.
Ano: 1996
URL: http://archimer.ifremer.fr/doc/00044/15516/12903.pdf

Etude des anomalies chromosomiques chez Crassostrea gigas - Contrat de Plan Région Poitou-Charentes 2000-2006. Convention 2000
Provedor de dados: 5
Autores: Lapegue, Sylvie; Mccombie, Helen; Leitao, Alexandra; Bouilly, Karine; Sabatier, Sebastien; Heurtebise, Serge; Boudry, Pierre; Thiriot Quievreux, Catherine; Gerard, Andre.
Divers travaux menés depuis 1984 montrent qu’il existe, chez l’huître creuse Crassostrea gigas, des cellules montrant un nombre anormal de chromosomes (2n = 19, 18 ou même 17 au lieu de 2n = 20). Le niveau d’aneuploïdie est déterminé par le décompte des chromosomes à partir de suspensions cellulaires de tissu branchial. Le pourcentage de cellules aneuploïdes est toujours significativement supérieur dans les "lots de queue", c'est-à-dire les huîtres présentant des croissances plus faibles, et peut atteindre plus de 30 %. Récemment, une étude réalisée au sein du programme européen « Genephys » a démontré que plus de 50 % de la variance pour la vitesse de croissance était liée au taux d’aneuploïdie. Cependant, de nombreux points concernant ce phénomène...
Tipo: Text
Palavras-chave: Génétique; Chromosomes; Aneuploïdie; Huîtres creuses; Crassostrea gigas.
Ano: 2001
URL: http://archimer.ifremer.fr/doc/00043/15474/12853.pdf

Trans-Atlantic distribution of a mangrove oyster species revealed by 16S mtDNA and karyological analyses
Provedor de dados: 5
Autores: Lapegue, Sylvie; Boutet, Isabelle; Leitao, Alexandra; Heurtebise, Serge; Garcia, P; Thiriot Quievreux, C; Boudry, Pierre.
Three species of man.-rove oysters, Crassostrea rhizophorae, C. brasiliana, and C. gasar, have been described along the Atlantic shores of South America and Africa. Because the distribution of these molluscs is of great biological and commercial interest, their taxonomy and distribution deserve further clarification. Therefore, 15 populations were sampled from both continents. Their 16S mitochondrial polymorphism was studied by sequencing and PCR-RFLP analysis. Two haplotypes were identified. Haplotype a was the only one observed in Africa, but it was also observed in South America together with haplotype b. Because C. gasar is the only mangrove oyster identified on the west coast of Africa, haplotype a was attributed to this species, which has thus been...
Tipo: Text
Palavras-chave: Mangrove oyster; Crassostrea gasar; Karyotype; Phylogeography; 16S mitochondrial DNA.
Ano: 2002
URL: http://archimer.ifremer.fr/doc/2002/publication-2790.pdf

QTL for resistance to summer mortality and OsHV-1 load in the Pacific oyster (Crassostrea gigas)
Provedor de dados: 5
Autores: Sauvage, Christopher; Boudry, Pierre; De Koning, D. -j.; Haley, C. S.; Heurtebise, Serge; Lapegue, Sylvie.
P&gt;Summer mortality is a phenomenon severely affecting the aquaculture production of the Pacific oyster (Crassostrea gigas). Although its causal factors are complex, resistance to mortality has been described as a highly heritable trait, and several pathogens including the virus Ostreid Herpes virus type 1 (OsHV-1) have been associated with this phenomenon. A QTL analysis for survival of summer mortality and OsHV-1 load, estimated using real-time PCR, was performed using five F-2 full-sib families resulting from a divergent selection experiment for resistance to summer mortality. A consensus linkage map was built using 29 SNPs and 51 microsatellite markers. Five significant QTL were identified and assigned to linkage groups V, VI, VII and IX. Analysis of...
Tipo: Text
Palavras-chave: Crassostrea gigas; Herpes virus; Pacific oyster; QTL mapping; Summer mortality.
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00011/12241/9063.pdf

Impact de la dissémination accidentelle d'huîtres tétraploïdes : potentiel de dispersion et succès reproducteur
Provedor de dados: 5
Autores: Lapegue, Sylvie; Heurtebise, Serge; Cornette, Florence; Brizard, Raphael; Sharbel, Tim; Boudry, Pierre.
L'ostréiculture française réalise une production très largement extensive, basée sur le recrutement de juvéniles dans le milieu naturel et la production sur des sites côtiers. Ainsi, l’huître est l’une des rares espèces cultivées à être encore considéré par le consommateur comme un produit alimentaire totalement « naturel ». L'utilisation de la polyploïdisation a permis d'ouvrir une nouvelle voie dans cette filière, en permettant d'obtenir des animaux à croissance plus rapide, plus homogènes et stériles. Cette dernière caractéristique est un gage de qualité gustative et permet aussi de positionner ce produit en priorité sur les mois en "r" traditionnellement bannis à cause de la présence de laitance. Le but principal de la triploïdisation a été atteint:...
Tipo: Text
Palavras-chave: Génétique; Triploïdisation; Tétraploïde; Impact; Environnement; Bivalvia; Crassostrea gigas.
Ano: 2004
URL: http://archimer.ifremer.fr/doc/00043/15460/12835.pdf

Etudes des bases génétiques et de la varibailité des caractères physiologiques impliqués dans la croissance chez l'huître creuse Crassostrea gigas
Provedor de dados: 5
Autores: Collet, Bertrand; Boudry, Pierre; Bougrier, Serge; Heurtebise, Serge; Phelipot, Pascal; Ledu, Christophe; Morand, Berenice; Heral, Maurice; Gerard, Andre.
A European programme (GENEPHYS) was launched in 1996 to study the genetic basis and variability of physiological characters involved in growth in Crassostrea gigas. This research programme was a prerequisite for any genetic selection programme on physiological functions. Research groups from Ireland, England, Greece and France are involved in this project and participate in genetic analyses (allozymes and microsatellites) and physiological analyses (ecophysiology, enzymology, stress proteins). Ifremer at La Tremblade was responsible for producing the biological material while Ifremer-CREMA (human-influenced marine ecosystems research centre) was responsible for carrying out non-destructive physiological analyses.
Tipo: Text
Palavras-chave: Sélection génétique; Croissance; Crassostrea gias; Génétique.
Ano: 1997
URL: http://archimer.ifremer.fr/doc/1997/acte-3243.pdf

Acclimatation de nouvelles espèces d'huîtres creuses du genre Crassostrea : hybridation et conservatoire de souches. Contrat Région Poitou-Charentes 1999 - Convention 99 RPC-A-203 "Génétique"
Provedor de dados: 5
Autores: Boudry, Pierre; Heurtebise, Serge; Lapegue, Sylvie; Ledu, Christophe; Phelipot, Pascal; Gerard, Andre.
Dans le cadre du plan Etat-Région 1994-1998, un programme de conservatoire de souches a été initié au Laboratoire IFREMER de La Tremblade. Ce conservatoire a pour objectif d'acclimater différentes espèces ou souches d'huîtres creuses (genres Crassostrea et Saccostrea) afin d'acquérir des connaissances sur ces espèces et d'évaluer l'intérêt qu'elles pourraient présenter pour l'ostréiculture locale. L'importation d'espèces étrangères sur les côtes françaises est désormais strictement réglementée: la dissémination d'une nouvelle espèce peut se révéler néfaste d'un point de vue écologique et nuire aux productions existantes. De plus, ces huîtres peuvent être porteuses de pathogènes qui, s'il venaient à se répandre dans le milieu, pourraient toucher les stocks...
Tipo: Text
Palavras-chave: Génétique; Acclimatation; Croisement; Crassostrea gigas; Crassostrea ariakensis; Crassostrea sikamea; Hybridation.
Ano: 1999
URL: http://archimer.ifremer.fr/doc/00044/15514/12901.pdf

Historique, expériences en cours et perspectives du programme de sélection de l'huître plate Ostrea edulis pour la résistance à la bonamiose
Provedor de dados: 5
Autores: Barre, Marc; Naciri, Yamama; Boudry, Pierre; Goyard, Emmanuel; Launey, Sophie; Cochennec, Nathalie; Heurtebise, Serge; Ledu, Christophe; Phelipot, Pascal; Gerard, Andre.
The Aquaculture and Pathological Genetics (GAP) laboratory at La Tremblade has been monitoring three populations of European flat oyster since 1985 or 1989 under selection for higher survival rates in the presence of the parasite Bonamia ostrea. One selection cycle (1985 population) or two (1989 populations) were carried out by mass selection by placing the oysters for several years in a highly infested zone, and then harvesting spawns from survivors.
Tipo: Text
Palavras-chave: Huîtres plates; Génétique; Selection génétique; Ostrea edulis.
Ano: 1997
URL: http://archimer.ifremer.fr/doc/1997/acte-3244.pdf

Morphological and mitochondrial DNA characteristics of two cultured species of cupped-oysters (Bivalvia : Ostreidae) in Hong Kong : towards a significant taxonomic name change
Provedor de dados: 5
Autores: Lam, Katherine; Morton, Brian; Boudry, Pierre; Heurtebise, Serge.
Cultured cupped-oysters species in Hong Kong have rarely been differentiated properly due to similarities in shell characters. This study attempted to characterize putative Crassostrea gigas and Crassostrea ariakensis by morphological and genetic means. Allometric ratios of shell height/length and depth/length were significantly different between the two species. Restriction fragment length polymorphism (RFLP) of a PCR amplified mitochondrial COI (cytochrome oxidase I) fragment was used to examine genetic differentiation between putative C. gigas from Hong Kong and previously studied populations of Crassostrea from Europe, Japan and Taiwan. The two Hong Kong species are not only morphologically, but also genetically different from each other in terms of...
Tipo: Text
Palavras-chave: Oysters; Hong Kong Crassostrea; Characteristics; Genetic population; Crassostrea ariakensis; Crassostrea gigas.
Ano: 2003
URL: http://archimer.ifremer.fr/doc/2003/acte-2844.pdf

Génomique de l'adaptation de l'huître creuse du Pacifique, Crassostrea gigas, dans le cadre de son expansion géographique
Provedor de dados: 5
Autores: Rohfritsch, Audrey; Bierne, Nicolas; Boudry, Pierre; Heurtebise, Serge; Lapegue, Sylvie.
Originating from the north eastern Asia, Crassostrea gigas has been introduced and translocated, mainly for aquaculture purpose, into several European countries (from Norway to Portugal and in the Mediterranean Sea) (1). Although highly variable, the invasiveness pattern of C. gigas has been demonstrated in several countries and therefore considered as a pest or a noxious species in those areas (2 and 3).  Our project aims at identifying the characteristics of such a flourishing species: can its success be explained by chance and/or global warming only or does it exhibit a more important potential of adaptation than other species? Therefore we developed a population genomics approach, known as "genome scan". It corresponds to the study of numerous loci...
Tipo: Text
Palavras-chave: Pacific oyster; Invasive; AFLP; Genome scan; Crassostrea gigas.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/acte-4542.pdf

PERLE - Programme d'expérimentation et de recherche sur l'huître plate Ostrea edulis - Rapport final des sous-programmes 2 et 3
Provedor de dados: 5
Autores: Arzul, Isabelle; Baillon, Laury; Boudry, Pierre; Bouget, Jean-francois; Chollet, Bruno; Cochet, Helene; Couraleau, Yann; Dubreuil, Christine; Duchene, Julie; Dutartre, Julie; Garcia, Celine; Heloury, Purotu; Heurtebise, Serge; Huchet, Eve; Hussenot, Mathieu; Langlade, Aime; Ollier, Simon; Omnes, Emmanuelle; Pouvreau, Stephane; Roby, Charlotte; Serpin, Delphine; Viard, Frederique; Wacrenier, Candice; Lapegue, Sylvie.
L’huître plate (Ostrea edulis) est une espèce endémique des côtes européennes, elle est présente depuis des millions d’années. Son aire de répartition est très vaste allant de la Norvège jusqu’au Maroc. Elle est également présente en mer Méditerranée, en mer Noire, mais également au Canada, aux Etats-Unis ou encore en Afrique du Sud, à la suite d'introductions pour des raisons aquacoles. En France, à partir de la fin des années 70, de fortes mortalités ont été constatées sur les bancs naturels et en élevage, faisant rapidement chuter la production de 20 000 tonnes à 2 000 tonnes. Ces mortalités ont été attribuées à deux maladies parasitaires : la marteiliose (due au parasite Marteilia refringens) et la bonamiose (due au parasite Bonamia ostreae). Trente...
Tipo: Text
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00247/35815/34328.pdf

Establishment of a genetic map in the European flat oyster Ostrea edulis L.
Provedor de dados: 5
Autores: Lallias, Delphine; Boudry, Pierre; Heurtebise, Serge; Lapegue, Sylvie; Beaumont, Andy.
The flat oyster Ostrea edulis is the species endemic from European coasts, Atlantic and Mediterranean. It has been exploited since Roman times in Europe. But its production decreased from around 20000 tons in the 1950ies to 1500 tons nowadays because of two successive diseases due to the intracellular parasites Marteilia refringens and Bonamia ostreae. Since 1985, Ifremer initiated a program of selection to produce families of oysters tolerant to Bonamia. In this context, a further step is to identify QTLs of resistance to this parasite. Therefore, a genetic map is first now being built. The establishment of a genetic linkage map will represent the basement for the mapping of QTLs, with the ultimate objective to implement marker-assisted selection in O....
Tipo: Text
Palavras-chave: Genetic map; Ostrea edulis; Genetic; European flat oyster.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/acte-3472.pdf

Essai d'acclimatation de Crassostrea virginica et performances biologiques comparées avec Crassostrea gigas (2ème partie)
Provedor de dados: 5
Autores: Gerard, Andre; Naciri, Yamama; Peignon, Jean-marie; Ledu, Christophe; Phelipot, Pascal; Baud, Jean-pierre; Heurtebise, Serge; Nourry, Max; Renault, Tristan; Cochennec, Nathalie; Zanette, Yvan; Blachier, Philippe.
Deuxième volet du programme d'essai d'acclimatation de l'huître américaine Crassostrea virginica, ce rapport présente les actions et les résultats de l'année 1993 qui a été consacrée au suivi des performances comparées de croissance des populations de C. gigas et C. virginica produites en 1992, ceci en trois sites différents: • dans le bassin de Marennes-Oléron en claire et sur estran, à la station IFREMER de Bouin en élevage intensif, au CREAA sur l'Ile d'Oléron en marais extensif. Au terme de cette seconde année d'étude, il est d'ores et déjà possible d'affirmer que que la souche anglaise de C. virginica étudiée ne présente pas de caractéristiques de croissance susceptibles de la rendre compétitive par rapport au témoin Crassostrea gigas et cela d'autant...
Tipo: Text
Palavras-chave: Mollusques; Acclimatation; Crassostrea virginica; Crassostrea gigas; Génétique; Pathologie.
Ano: 1994
URL: http://archimer.ifremer.fr/doc/00077/18846/16419.pdf

Suivi de la reproduction des mollusques d'élevage 1987
Provedor de dados: 5
Autores: Heurtebise, Serge; Ratiskol, Jacqueline.
Comme précédemment en 1985 et 1986, le suivi de la reproduction des huîtres et des moules a été assuré par le CSRU de La Tremblade pour les secteurs clefs de la production du naissain que sont l'embouchure de la Charente, la Seudre et le bassin de Marennes-Oléron.
Tipo: Text
Palavras-chave: Reproduction; Captage; Naissain; Crassostrea gigas; Mytilus edulis; Marennes Oléron.
Ano: 1987
URL: http://archimer.ifremer.fr/doc/00072/18287/15859.pdf

Relationship between pre- and post-metamorphic growth in the Pacific oyster Crassostrea gigas (Thunberg)
Provedor de dados: 5
Autores: Collet, Bertrand; Boudry, Pierre; Thebault, Anne; Heurtebise, Serge; Morand, Berenice; Gerard, Andre.
Twenty male and 20 female parental oysters, originating from four sites located along the French Atlantic coast, were crossed together. The 400 crosses were performed separately and then pooled to give a batch of larvae with a large genetic base. Successive sieving after 17 days at 23°C enabled the separation of the largest larvae from the batch. These larvae (i.e., sieving groups) were left to metamorphose and fix onto flat PVC collectors changed daily. Four groups of larvae representing distinctly different growth rates were successively separated during the attachment and metamorphosis of the whole population. This lasted 12 days, from the 17th day to the 29th after fertilisation. A part of each sieving group was settled separately as replicates on...
Tipo: Text
Palavras-chave: Settlement; Metamorphosis; Crassostrea gigas; Growth.
Ano: 1999
URL: http://archimer.ifremer.fr/doc/1999/publication-468.pdf

Etude des anomalies chromosomiques chez Crassostrea gigas
Provedor de dados: 5
Autores: Lapegue, Sylvie; Mccombie, Helen; Leitao, Alexandra; Bouilly, Karine; Sabatier, Sebastien; Heurtebise, Serge; Boudry, Pierre; Thiriot, Catherine; Gerard, Andre.
Divers travaux menés depuis 1984 montrent qu'il existe, chez l'huître creuse Crassostrea gigas, des cellules montrant un nombre anormal de chromosomes (2n = 19, 18 ou même 17 au lieu de 2n = 20). Le niveau d'aneuploïdie est déterminé par le décompte des chromosomes à partir de suspensions cellulaires de tissu branchial. Le pourcentage de cellules aneuploïdes est toujours significativement supérieur dans les "lots de queue", c'est-à-dire les huîtres présentant des croissances plus faibles, et peut atteindre plus de 30 %. Récemment, une étude réalisée au sein du programme européen « Genephys » a démontré que plus de 50 % de la variance pour la vitesse de croissance était liée au taux d'aneuploïdie. Cependant, de nombreux points concernant ce phénomène...
Tipo: Text
Palavras-chave: Génétique; Chromosomes; Anomalies; Aneuploïdie; Atrazine; Huîtres creuses; Crassostrea gigas.
Ano: 2002
URL: http://archimer.ifremer.fr/doc/00199/31013/29417.pdf

Compte rendu sur la reproduction des huîtres et des moules dans le bassin de Marennes Oléron pour l'année 1987
Provedor de dados: 5
Autores: Heurtebise, Serge; Ratiskol, Jacqueline.
Comme précédemment en 1985 et 1986, le suivi de la reproduction des huîtres et des moules a été assuré par le CSRU de La Tremblade pour les secteurs clefs de la production du naissain que sont l'embouchure de la Charente, la Seudre et le bassin de Marennes-Oléron. Ce suivi a nécessité 40 sorties en mer, dont 21 à partir du Chapus ou de La Tremblade (moyens nautiques IFREMER) et 19 au départ de Port des Barques avec la location des services d'un professionnel. Les différents paramètres physico-chimiques (T°, S %, 02) ont été mesurés à l'occasion de chaque pêche de larves pour appréhender au mieux l'évolution de la reproduction et fournir des indications complémentaires sur les bulletins de numération. Cette année, 30 bulletins sont parvenus à la profession...
Tipo: Text
Palavras-chave: Reproduction; Larves; Crassostrea gigas; Mytilus edulis; Bassin de Marennes Oléron.
Ano: 1987
URL: http://archimer.ifremer.fr/doc/00049/16071/13533.pdf

Genetic Characterization of Cupped Oyster Resources in Europe Using Informative Single Nucleotide Polymorphism (SNP) Panels
Provedor de dados: 5
Autores: Lapegue, Sylvie; Heurtebise, Serge; Cornette, Florence; Guichoux, Erwan; Gagnaire, Pierre-alexandre.
The Pacific oyster, Crassostrea gigas, was voluntarily introduced from Japan and British Columbia into Europe in the early 1970s, mainly to replace the Portuguese oyster, Crassostrea angulata, in the French shellfish industry, following a severe disease outbreak. Since then, the two species have been in contact in southern Europe and, therefore, have the potential to exchange genes. Recent evolutionary genomic works have provided empirical evidence that C. gigas and C. angulata exhibit partial reproductive isolation. Although hybridization occurs in nature, the rate of interspecific gene flow varies across the genome, resulting in highly heterogeneous genome divergence. Taking this biological property into account is important to characterize genetic...
Tipo: Text
Palavras-chave: Cupped oysters; Population genetics; Introgression; Conservation; Shellfisheries.
Ano: 2020
URL: https://archimer.ifremer.fr/doc/00623/73544/72938.pdf

Bonamia-ostreae induced mortalities in one-year old European flat oysters Ostrea edulis: experimental infection by cohabitation challenge
Provedor de dados: 5
Autores: Lallias, Delphine; Arzul, Isabelle; Heurtebise, Serge; Ferrand, Sylvie; Chollet, Bruno; Robert, Maeva; Beaumont, Andrew; Boudry, Pierre; Morga, Benjamin; Lapegue, Sylvie.
Bonamiosis is a parasitic disease (causative agent: Bonamia ostreae) affecting the European flat oyster Ostrea edulis, responsible for a drastic decline in the aquaculture production of this oyster species. Therefore a selective breeding program for resistance to bonamiosis has been undertaken since 1985 bu Ifremer, leading to the production of several selected oyster families. In the present study, a 6-month cohabitation challenge experiment was performed in order to transmit the disease from wild oysters (injected with the parasite) to two tested families of oysters originating from the selective breeding program. Mortalities were checked daily, and ventricular heart smears were performed on dying or moribund oysters to detect the level of infection to...
Tipo: Text
Palavras-chave: Heart smear; Transmission; Cohabitation experiment; Bonamia ostreae; Ostrea edulis.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/acte-4535.pdf

Etude des anomalies chromosomiques chez Crassostrea gigas. Contrat Région Poitou-Charentes 2000-2006. Convention 99
Provedor de dados: 5
Autores: Lapegue, Sylvie; Mccombie, Helen; Leitao, Alexandra; Heurtebise, Serge; Boudry, Pierre; Thiriot Quievreux, Catherine; Gerard, Andre.
Divers travaux menés depuis 1984 montrent qu’il existe, chez l’huître creuse Crassostrea gigas, des cellules montrant un nombre anormal de chromosomes (2n = 19, 18 ou même 17 au lieu de 2n = 20). Le niveau d’aneuploïdie est déterminé par le décompte des chromosomes à partir de suspensions cellulaires de tissu branchial. Le pourcentage de cellules aneuploïdes est toujours significativement supérieur dans les "lots de queue", c'est-à-dire les huîtres présentant des croissances plus faibles, et peut atteindre plus de 30 %. Récemment, une étude réalisée au sein du programme européen « Genephys » a démontré que plus de 50 % de la variance pour la vitesse de croissance était liée au taux d’aneuploïdie. Cependant, de nombreux points concernant ce phénomène...
Tipo: Text
Palavras-chave: Génétique; Chromosomes; Aneuploïdie; Huîtres creuses; Crassostrea gigas.
Ano: 2000
URL: http://archimer.ifremer.fr/doc/00043/15473/12852.pdf

Phylogeographic study of the dwarf oyster, Ostreola stentina, from Morocco, Portugal and Tunisia: evidence of a geographic disjunction with the closely related taxa, Ostrea aupouria and Ostreola equestris
Provedor de dados: 5
Autores: Lapegue, Sylvie; Ben Salah, Ines; Batista, Frederico; Heurtebise, Serge; Neifar, L; Boudry, Pierre.
Despite the economic importance of oysters due to the high aquaculture production of several species, the current knowledge of oyster phylogeny and systematics is still fragmentary. In Europe, Ostrea edulis, the European flat oyster, and Ostreola stentina, the Provence oyster or dwarf oyster, are both present along the European and African, Atlantic and Mediterranean, coasts. In order to document the relationship not only between O. stentina and O. edulis, but also with the other Ostrea and Ostreola species, we performed a sequence analysis of the 16S mitochondrial fragment (16S rDNA: the large subunit rRNA-coding gene) and the COI fragment (COI: cytochrome oxidase subunit I). Oysters were sampled from populations in Portugal (two populations), Tunisia...
Tipo: Text
Palavras-chave: Tunisia; Portugal; Marocco; Ostreola stentina; Ostreola equestris; Ostrea aupouria; Phylogeographic study.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/publication-1912.pdf

A first generation genetic linkage map of the European flat oyster Ostrea edulis (L.) based on AFLP and microsatellite markers
Provedor de dados: 5
Autores: Lallias, Delphine; Beaumont, A.r.; Haley, C.s.; Boudry, Pierre; Heurtebise, Serge; Lapegue, Sylvie.
This study presents the first genetic linkage map for the European flat oyster Ostrea edulis. AFLP and 20 microsatellite markers were genotyped in a three-generation pedigree comprised of 2 grand-parents, 2 parents and 92 progeny. Chi-square goodness of fit tests revealed high segregation distortion, which was significant for 32.8% of markers. Sixteen microsatellites and 235 AFLPs (170 AFLPs type 1:1 and 65 AFLPs type 3:1) were used to build sex-specific linkage maps using CriMap software. The first parental map (P1) consisted of 104 markers grouped in nine linkage groups, spanning 471.2 cM with an average spacing of 4.86 cM. The second parental map (P2) consisted of 117 markers grouped in ten linkage groups (which equals the haploid chromosome number),...
Tipo: Text
Palavras-chave: Microsatellite; AFLP; Ostrea edulis; Flat oyster; Genetic linkage map.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/publication-2642.pdf

Génomique de l'adaptation de l'huître creuse, Crassostrea gigas, dans le contexte de sa récente expansion géographique en Europe
Provedor de dados: 5
Autores: Rohfritsch, Audrey; Bierne, Nicolas; Boudry, Pierre; Heurtebise, Serge; Cornette, Florence; Lapegue, Sylvie.
Originaire du nord de l’Asie, l'huître creuse, Crassostrea gigas, a été introduite et transférée, principalement pour l’aquaculture, dans plusieurs pays européens depuis le début des années 1970. Le caractère invasif de cette espèce a été observé au cours de la dernière décennie dans plusieurs pays et elle a même été considérée comme une espèce nuisible dans certaines régions, telles que la mer de Wadden. Dans le cadre du projet HiFlo, soutenu par l’ANR, nous avons pour objectif d'identifier les caractéristiques d'une telle espèce florissante: son succès peut-il être expliqué par le hasard des opportunités (expansion de leur niche écologique) ou est-il lié à des processus évolutifs, ce qui impliquerait une adaptation génétique de l'espèce à de nouvelles...
Tipo: Text
Palavras-chave: Huîtres; Crassostrea gigas; Génétique; Génétique des populations.
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00014/12552/9418.pdf

Diversification des productions conchylicoles en Poitou-Charentes. L'élevage du pétoncle noir Chramys Varias
Provedor de dados: 5
Autores: Bodoy, Alain; Garnier, Jacqueline; Heurtebise, Serge; Nollet, V.; Chagot, Dominique.
La croissance et la reproduction du pétoncle noir, Chlamys varia, ont été suivies sur un cycle biologique complet, dans un marais maritime. Cette croissance s'est avérée être nettement, plus rapide qu'en rade de Brest, puisque la taille commerciale de 35 mm a été atteinte en un an. Les individus se sont reproduits au bout de la même période. Ceci montre qu'il est possible d'obtenir un cycle biologique très rapide. Lors du cycle de production, des mortalités importantes ont été constatées entre le prégrossissement en nurserie et la mise en élevage. Ces mortalités ne se sont pas renouvelées sur le cycle en cours d'étude. Un agent parasite nouveau a été décrit sur cette espèce, sans que les certitudes puissent être établies quant à son rôle pathogène....
Tipo: Text
Palavras-chave: Croissance; Composition biochimique; Elevage; Pétoncle; Marais maritime; Charente-Maritime.
Ano: 1991
URL: http://archimer.ifremer.fr/doc/00048/15905/13333.pdf

Development of SNP genotyping arrays in two shellfish species
Provedor de dados: 5
Autores: Lapegue, Sylvie; Harrang, Estelle; Heurtebise, Serge; Flahauw, Emilie; Donnadieu, C.; Gayral, P.; Ballenghien, M.; Genestout, L.; Barbotte, L.; Mahla, R.; Haffray, P.; Klopp, C.
Use of SNPs has been favored due to their abundance in plant and animal genomes, accompanied by the falling cost and rising throughput capacity for detection and genotyping. Here, we present in vitro (obtained from targeted sequencing) and in silico discovery of SNPs, and the design of medium-throughput genotyping arrays for two oyster species, the Pacific oyster, Crassostrea gigas, and European flat oyster, Ostrea edulis. Two sets of 384 SNP markers were designed for two Illumina GoldenGate arrays and genotyped on more than 1000 samples for each species. In each case, oyster samples were obtained from wild and selected populations and from three-generation families segregating for traits of interest in aquaculture. The rate of successfully genotyped...
Tipo: Text
Palavras-chave: Crassostrea gigas; GoldenGate technology; Ostrea edulis; Oysters; SNP genotyping.
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00172/28369/26677.pdf

Bases génétiques et plasticité de la croissance et de la survie chez l'huître creuse, Crassostrea gigas
Provedor de dados: 5
Autores: Ernande, Bruno; Boudry, Pierre; Heurtebise, Serge; Haure, Joel; Martin, Jean-louis.
Growth and survival are the two most interesting characters for oyster farming as they determine the biomass produced in the farms (growth x survival). Thus, a selection program aiming at improving these two characters could be of a great help. However, the oyster, as a sessile organism, is influenced by environmental variability (spatial and temporal) either natural or caused by the used oyster culture methods. Submitted to this variability, oysters must have evolved so as to adapt (short term) to this environmental variability.
Tipo: Text
Palavras-chave: Crassostrea gigas; Huîtres; Croissance; Plasticité; Génétique.
Ano: 2001
URL: http://archimer.ifremer.fr/doc/2001/acte-3279.pdf

Taxonomie de l'huître naine Ostreola stentina
Provedor de dados: 5
Autores: Lapegue, Sylvie; Ben Salah, Inès; Batista, Frederico; Heurtebise, Serge; Neifar, Lassad; Boudry, Pierre.
Despite the economic importance of numerous oyster species throughout the world, our knowledge of their systematics and phylogeography remains fragmentary. In Europe, the indigenous species, Ostrea edulis, suffered overexploitation as well as two successive parasitosis over the last century. However, this species is still cultivated in Europe, and especially in Spain and France. Its distribution area stretches from the Atlantic coasts of Europe to the north of Africa as well as around the Mediterranean and Black Sea. Its Mediterranean and South Atlantic distribution area is shared with another indigenous species, the dwarf oyster, Ostreola stentina. Its very small adult size (a few centimetres) makes its culture pointless from a commercial point of view....
Tipo: Text
Palavras-chave: Ostreola stentina; Oyster; Taxonomy; Ostreola stentina; Huître; Taxonomie.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/acte-3364.pdf

Combining Two-Stage Testing and Interval Mapping Strategies to Detect QTL for Resistance to Bonamiosis in the European Flat Oyster Ostrea edulis
Provedor de dados: 5
Autores: Lallias, Delphine; Gomez-raya, L.; Haley, C. S.; Arzul, Isabelle; Heurtebise, Serge; Beaumont, A. R.; Boudry, Pierre; Lapegue, Sylvie.
We have identified quantitative trait loci (QTL) in the flat oyster (Ostrea edulis) for resistance to Bonamia ostreae, a parasite responsible for the dramatic reduction in the aquaculture of this species. An F-2 family from a cross between a wild oyster and an individual from a family selected for resistance to bonamiosis was cultured with wild oysters injected with the parasite, leading to 20% cumulative mortality. Selective genotyping of 92 out of a total of 550 F-2 progeny (i.e., 46 heavily infected oysters that died and 46 parasite-free oysters that survived) was performed using 20 microsatellites and 34 amplification fragment length polymorphism primer pairs. Both a two-stage testing strategy and QTL interval mapping methods were used. The two-stage...
Tipo: Text
Palavras-chave: Statistical power; Survival analysis; QTL mapping; Disease resistance; Ostrea edulis.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/2009/publication-6682.pdf

Réseau de surveillance de la croissance et production de l'huître creuse Crassostrea gigas dans le bassin de Marennes-Oléron : bilan de 12 années de suivi (1986-1998)
Provedor de dados: 5
Autores: Le Moine, Olivier; Geairon, Philippe; Soletchnik, Patrick; Faury, Nicole; Goulletquer, Philippe; Robert, Stephane; Razet, Daniel; Heurtebise, Serge.
Thiis report aims to study the oyster Crwsosrrea gigas growth and production over a 12 years time' s span in the bay of Marennes-DIeron. Results of 12 years of oyster growlh did not show any significant oyster biological productivity decrease, nor trend. ln contrast, annual growth showed a large variability, which will be correlated with environmental conditions. Moreover, annual growth variability was greater than the variability due to site location,. besides the Ronce experimental site. The later, located in the south west part of the bay of Marennes-Dleron, has been the leading oyster growth site since 1989. The annual shell growlh variabiliry was greater than oyster dry meat weight fluctuation . Both 1990 and 1991 years were characterized by the...
Tipo: Text
Palavras-chave: Réseau de surveillance; Croissance; Composition biochimique; Mortalité; Crassostrea gigas; Bassin de Marennes-Oléron; Charente-Maritime; Monitoring network; Growth; Biochemical composition; Mortality rate; Crassostrea gigas; Bay of Marennes Oleron; Charente-Maritime.
Ano: 2000
URL: http://archimer.ifremer.fr/doc/00031/14244/11520.pdf

The taxonomic status and origin of the Portuguese oyster Crassostea angulata (Lamark, 1819)
Provedor de dados: 5
Autores: Batista, Frederico; Leitao, Alexandra; Huvet, Arnaud; Lapegue, Sylvie; Heurtebise, Serge; Boudry, Pierre.
The taxonomic status of the Portuguese oyster Crassostrea angulata and the Pacific oyster Crassostrea gigas has often been a matter of controversy. Based on larval shell morphology, experimental hybridisation and electrophoretic studies of enzyme polymorphism several authors have considered these two species as being synonymous. During the recent years, several genetic studies based on mitochondrial DNA and microsatellites data have provided evidences that the two taxa are genetically distinct although closely related. Furthermore, karyotype analyses have also highlighted the close genetic similarity of these taxa in comparison with other cupped oyster species. However, a comparative analysis of restriction enzymes ideograms revealed differences between...
Tipo: Text
Palavras-chave: Geographical origin; Phylogenetic; Genetic; Taxonomic status; Crassostrea angulata; Oyster.
Ano: 2005
URL: http://archimer.ifremer.fr/doc/2005/acte-3433.pdf

Etude des ressources génétiques chez les huîtres creuses
Provedor de dados: 5
Autores: Boudry, Pierre; Heurtebise, Serge; Lapegue, Sylvie; Lam, Katherine; Mccombie, Helen; Leitao, Alexandra; Gerard, Andre.
L'étude de la variabilité génétique de l'huître creuse Crassostrea gigas est un point essentiel dans la mise en place de programmes de gestion des ressources génétiques et de sélection chez cette espèce. C. gigas, originaire du Japon, a été introduite dans de nombreuses régions du monde et représente aujourd'hui plus de 95 'ro de la production mondiale d'huîtres. Introduite en France au début des années 1970 pour remplacer l'huître portugaise Crassostrea angulata, c'est aujourd'hui une ressource de première importance pour notre région. Malgré la maîtrise du cycle de reproduction de l'espèce et son importance commerciale, elle n'a pas été génétiquement améliorée et on peut donc considérer les stocks comme « sauvages» ou « non domestiqués ». De plus, la...
Tipo: Text
Palavras-chave: Génétique; Huîtres creuses; Crassostrea gigas; Crassostrea gasar; Variabilité génétique.
Ano: 2002
URL: http://archimer.ifremer.fr/doc/00199/31008/29411.pdf

Amélioration de la qualité chez l'huître creuse Crassostrea gigas par sélection de souches performantes - Contrat de Plan Etat Région Poitou-Charentes 1994-1998 - Rapport année 1995
Provedor de dados: 5
Autores: Boudry, Pierre; Naciri, Yamama; Heral, Maurice; Collet, Bertrand; Bougrier, Serge; Geffard, Olivier; Ledu, Christophe; Phelipot, Pascal; Heurtebise, Serge; Gerard, Andre.
Le programme de recherche présenté ci-dessous est le fruit d'une réflexion conjointe entre généticiens et physiologistes afin de mettre sur pied un ensemble d'expériences permettant d'étudier les bases génétiques de la variabilité des caractères impliqués dans la croissance chez l'huître creuse, Crassostrea gigas.
Tipo: Text
Palavras-chave: Génétique; Sélection génétique; Génétique des populations; Croissance; Crassostrea gigas.
Ano: 1995
URL: http://archimer.ifremer.fr/doc/00044/15518/12905.pdf

QTL mapping for resistance to bonamiosis in the European flat oyster Ostrea edulis
Provedor de dados: 5
Autores: Lallias, Delphine; Beaumont, Andy; Haley, Chris; Heurtebise, Serge; Boudry, Pierre; Lapegue, Sylvie.
A breeding programme has been undertaken by Ifremer to produce selected Ostrea edulis families which are resistant to Bonamia ostreae, a parasite which is the most significant constraint on aquaculture of this oyster. Using such families, we aimed to identify Quantitative Trait Loci (QTLs) of resistance/susceptibility to bonamiosis. We used an F2 family derived from a bi-parental cross between a wild oyster and an individual from one of the selected oyster families. A total of 550 F2 individuals from the family cohabited with wild oysters that were injected with the parasite. Mortality was checked daily over six months and heart smears were used on moribund or dying oysters to detect the presence and level of infection of the parasite. Ninety two F2...
Tipo: Text
Palavras-chave: QTLs; Flat oyster; Ostrea edulis; Bonamia ostreae; Genetic; QTL.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/acte-3726.pdf

Obtention de souches conchylicoles performantes par polyploïdisation (4ème partie)
Provedor de dados: 5
Autores: Gerard, Andre; Naciri, Yamama; Peignon, Jean-marie; Ledu, Christophe; Phelipot, Pascal; Bodoy, Alain; Heurtebise, Serge; Garnier, Jacqueline; Baud, Jean-pierre; Nourry, Max; Haure, Joel; Martin, Anne-genevieve; Claude, Serge; Barret, Jean; Devauchelle, Nicole; Joly, Jean-pierre; Goulletquer, Philippe; Coatanea, Denis; Oheix, Jocelyne; Zanette, Yvan; Blachier, Philippe.
This fourth part of the polyploidisation program reports the experiments that were conducted in 1992 and that were devoted to : - the acquiring of a new method using an epifluroescent microscope - the development o a new technique for inducing polyploidy - the triploidy induction for the Europena clam Ruditapes decussatus - the settlement of performance controls conducted on diploid and triploid populations of Ostrea edulis - results of performance controls conducted on diploid and triploid populations of Crassostrea gigas and Ruditapes philippinarum
Tipo: Text
Palavras-chave: Ruditapes decussatus; Ruditapes philippinarum; Ostrea edulis; Crassostra gigas; Cytongenetics; Polyploidy; Ruditapes decussatus; Ruditapes philippinarum; Ostrea edulis; Crassostrea gigas; Cytogénétique; Polyploïdie.
Ano: 1993
URL: http://archimer.ifremer.fr/doc/1993/rapport-3859.PDF

Population genomics shed light on the demographic and adaptive histories of European invasion in the Pacific oyster, Crassostrea gigas
Provedor de dados: 5
Autores: Rohfritsch, Audrey; Bierne, Nicolas; Boudry, Pierre; Heurtebise, Serge; Cornette, Florence; Lapegue, Sylvie.
Crassostrea gigas originated from the Pacific coast of Asia, but was introduced into several European countries in the early 1970s. Natural populations have now spread across the length of the western seaboard of Europe. To elucidate the demographic and selective processes at play during this rapid expansion, genome-scan analysis was performed on different populations. High diversities and low differentiation were observed overall, but significant genetic differentiation was found among newly established populations and between the newly established northern group and a nearly panmictic group composed of southern European populations and a population from Japan. Loss of genetic diversity was also seen in the north, likely caused by founder events during...
Tipo: Text
Palavras-chave: AFLPs; Crassostrea gigas; Genome scan; Invasive species; Microsatellites; SNPs.
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00149/25977/24070.pdf

Application of high-throughput sequencing to population differentiation in the cupped oysters Crassostrea angulata/C. gigas
Provedor de dados: 5
Autores: Gagnaire, Pierre-alexandre; Cornette, Florence; Heurtebise, Serge; Auge, M. T.; Bierne, Nicolas; Lapegue, Sylvie.
The cupped oysters Crassostrea angulata and Crassostrea gigas have been sequentially introduced in Europe from their native Pacific area for aquaculture purposes. Ongoing viral epidemic disease is responsible for large losses in oyster aquaculture production throughout Europe. In parallel with current efforts in developing selection programs for disease resistance, it becomes critically important to characterize genetic diversity patterns in both native and introduced areas. With the advent of next generation sequencing techniques, the power to resolve the fine-scale genetic structure at a genome-wide scale opens to new perspectives towards genetic resource management. Here, we present a genome-wide screen of genetic variation that combines SNP genotyping...
Tipo: Text
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00167/27816/26009.pdf

Analysis of genome-wide differentiation between native and introduced populations of the cupped oysters Crassostrea gigas and Crassostrea angulata
Provedor de dados: 5
Autores: Gagnaire, Pierre-alexandre; Lamy, Jean-baptiste; Cornette, Florence; Heurtebise, Serge; Degremont, Lionel; Flahauw, Emilie; Boudry, Pierre; Bierne, Nicolas; Lapegue, Sylvie.
The Pacific cupped oyster is genetically subdivided into two sister taxa, Crassostrea gigas and C. angulata, which are in contact in the north-western Pacific. The nature and origin of their genetic and taxonomic differentiation remains controversial due the lack of known reproductive barriers and the high degree of morphologic similarity. In particular, whether the presence of ecological and/or intrinsic isolating mechanisms contributes to species divergence is unknown. The recent co-introduction of both taxa into Europe offers a unique opportunity to test how genetic differentiation is maintained under new environmental and demographic conditions. We generated a pseudo-chromosome assembly of the Pacific oyster genome using a combination of BAC-end...
Tipo: Text
Palavras-chave: Cupped oysters; Genome assembly; Species divergence; Reproductive barriers; Recombination rate.
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00455/56622/58337.pdf

L'élevage hivernal du pétoncle noir Chlamys varia sur les côtes de Charente-Maritime. Résultats préliminaires en claire et en mer ouverte
Provedor de dados: 5
Autores: Bodoy, Alain; Garnier, Jacqueline; Heurtebise, Serge.
Dans le cadre d'un programme de recherche financé partiellement par la région Poitou-Charentes, un élevage expérimental du pétoncle noir, Chlamys varia, a été réalisés dans deux sites aquacoles. L'un concerne la mer ouverte, au moyen de filière d'élevage de type subflottant, l'autre les bassins en terre du marais maritime (claire). Une production en écloserie et un prégrossissement intensif ayant été réalisé, le naissain d'une taille d'environ 18 mm a fait l'objet d'un élevage hivernal, selon un plan expérimental qui comprenait un facteur relatif au milieu d'élevage et un facteur relatif à la densité. D'importantes mortalités ont été constatées juste après la mise en élevage. Elles semblent être en relation avec le transfert des individus entre le site de...
Tipo: Text
Palavras-chave: Aquaculture; Pectinidés; Pétoncle; Filière d'élevage; Marais maritime.
Ano: 1990
URL: http://archimer.ifremer.fr/doc/00031/14205/11474.pdf

Genetic characterisation of oyster populations along the north-eastern coast of Tunisia
Provedor de dados: 5
Autores: Dridi, S.; Romdhane, M. S.; Heurtebise, Serge; El Cafsi, M.; Boudry, Pierre; Lapegue, Sylvie.
The taxonomy of oysters has been traditionally based on characteristics of the shell. More recently, the analysis of protein and DNA polymorphism has provided a means to overcome difficulties in distinguishing the different species of oysters based solely on shell morphology. In order to identify oysters of the Tunisian north-east coast, we sequenced a 16S rRNA mitochondrial fragment from 68 oysters sampled from the Bizert Lagoon and the Gulf of Hammamet in northern Tunisia. Comparison of oyster 16S rRNA sequences available in GenBank showed the presence of both Ostreola stentina and Crassostrea gigas in our samples, which could not be detected on the basis of shell morphology only. These data confirmed that C. gigas, a non-native species, is now...
Tipo: Text
Palavras-chave: 16S rRNA; Crassostrea gigas; Genetics; Mediterranean Sea; Ostreola stentina; Polymorphism.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/00000/11063/7820.pdf

Acclimatation de nouvelles espèces d'huîtres creuses du genre Crassostrea: hybridation et conservatoire de souches. Amélioration de la qualité chez l'huîtres creuses Crassostrea gigas par sélection de souches performantes - Contrat de Plan Etat Région Poitou-Charentes 1994-1998 - Convention 97 RPC-R-54 "Génétique" - Rapport année 1997
Provedor de dados: 5
Autores: Boudry, Pierre; Lapegue, Sylvie; Goyard, Emmanuel; Heurtebise, Serge; Ledu, Christophe; Phelipot, Pascal; Gerard, Andre.
Les objectifs pour l'année 1997 étaient les suivants : • Poursuivre les premières reproductions des espèces pour lesquelles nous disposons de géniteurs GO. L'objectif est toujours de limiter le stockage d'animaux importés en produisant une descendance simultanément à des témoins C. gigas qui serviront de comparaison. • Tenter des hybridations inter-spécifiques des différents taxons présents au sein du conservatoire en fonction des connaissances disponibles dans la littérature sur ce sujet (voir pour revue Gaffney et Allen, 1993). • Importer de nouvelles souches ou espèces. Élargir la gamme des espèces présentes au sein du conservatoire de souches (les capacités d'accueil de la salle de quarantaine étant limitée).
Tipo: Text
Palavras-chave: Génétique; Génétique des Populations; Acclimatation; Hybridation; Marqueurs génétiques; Sélection génétique; Crassostrea gigas.
Ano: 1997
URL: http://archimer.ifremer.fr/doc/00044/15519/12906.pdf

Complete mitochondrial DNA sequence of the European flat oyster Ostrea edulis confirms Ostreidae classification
Provedor de dados: 5
Autores: Danic-tchaleu, Gwenaelle; Heurtebise, Serge; Morga, Benjamin; Lapegue, Sylvie.
Background  Because of its typical architecture, inheritance and small size, mitochondrial (mt) DNA is widely used for phylogenetic studies. Gene order is generally conserved in most taxa although some groups show considerable variation. This is particularly true in the phylum Mollusca, especially in the Bivalvia. During the last few years, there have been significant increases in the number of complete mitochondrial sequences available. For bivalves, 35 complete mitochondrial genomes are now available in GenBank, a number that has more than doubled in the last three years, representing 6 families and 23 genera. In the current study, we determined the complete mtDNA sequence of Ostrea edulis, the European flat oyster. We present an analysis of features of...
Tipo: Text
Ano: 2011
URL: http://archimer.ifremer.fr/doc/00045/15628/13031.pdf

Phylogeography of mangrove oysters from the Southern Atlantic ocean : Crassostrea gasar and Crassostre rhizophorae
Provedor de dados: 5
Autores: Lapegue, Sylvie; Boutet, Isabelle; Leitao, Alexandra; Thiriot-quievreux, Catherine; Heurtebise, Serge; Garcia, P.; Boudry, Pierre.
Mangrove oysters encompass several species and their taxomony is unclear. Among then, Crassostrea rhizophorae has been described along the Central and South American Atlantic coasts and Crassostrea gasar along African Atlantic coasts. Most taxonomic studies have so far been based on morphological criteria which are particularly susceptible to plasticity and new insight can be provided by molecular markers. The present work aims to provide information on the phylogeography of these two taxa.
Tipo: Text
Palavras-chave: Genetic; Phylogeography; Crassostrea rhizophorae; Crassostrea gasar; Mangrove oysters.
Ano: 2000
URL: http://archimer.ifremer.fr/doc/2000/acte-3496.pdf

Diversification des productions conchylicoles en Poitou-Charentes. L'élevage du pétoncle noir Chlamys Varia
Provedor de dados: 5
Autores: Bodoy, Alain; Garnier, Julien; Heurtebise, Serge; Nollet, V.; Chagot, D.
La croissance et la reproduction du pétoncle noir, Chlamys varia, ont été suivies sur un cycle biologique complet, dans un marais maritime. Cette croissance s'est avérée être nettement plus rapide qu'en rade de Brest, puisque la taille commerciale de 35 mm a été atteinte en un an. Les individus se sont reproduits au bout de la même période. Ceci montre qu'il est possible d'obtenir un cycle biologique très rapide.
Tipo: Text
Ano: 1991
URL: http://archimer.ifremer.fr/doc/00080/19152/16747.pdf

Past and present geographical distribution of populations of Portuguese (Crassostrea angulata) and Pacific (C. gigas) oysters along the European and north African Atlantic coats
Provedor de dados: 5
Autores: Fabioux, Caroline; Huvet, Arnaud; Lapegue, Sylvie; Heurtebise, Serge; Boudry, Pierre.
Today, it is commonly accepted that C. angulata and C. gigas are a single species according to morphological, genetic and experimental hybridization data. Following the viral disease that affected C. angulata and the subsequent expansion of C. gigas in Europe, it was of interest to examine the geographical distribution of both taxa in Europe. We studied the genetic composition of seventeen populations of cupped oysters, sampled in France, Spain, Portugal, Morocco and Italy, using a diagnostic marker, the mitochondrial Cytochrome Oxydase I gene. Results showed two distinct geographical zones. All the populations located in the northern part of the European sampled zone were mainly composed of C. gigas haplotypes whereas populations from southern Europe and...
Tipo: Text
Palavras-chave: Geographical distribution; Crassostrea angulata; Crassostrea gigas; Distribution géographique; Crassostrea angulata; Crassostrea gigas.
Ano: 2002
URL: http://archimer.ifremer.fr/doc/2002/publication-2785.pdf

Compte rendu de la réunion des 14 et 15 Avril 1993 - La Tremblade - Réseau Génétique Mollusques (REGEMO)
Provedor de dados: 5
Autores: Gerard, Andre; Naciri, Yamama; Coatanea, Denis; Martin, Anne-genevieve; Barret, Jean; Claude, Serge; Heurtebise, Serge; Devauchelle, Nicole; Joly, Jean-pierre; Goulletquer, Philippe; Baud, Jean-pierre; Nourry, Max; Bougrier, Serge; Renault, Tristan; Cochennec, Nathalie; Chollet, Bruno; Le Deuff, Rose-marie; Peignon, Jean-marie.
La discussion générale organisée à la fin de la deuxième journée a fait ressortir les points suivants: 1- Les participants se sont déclarés satisfaits de la tenue de la réunion, tant dans son déroulement, que dans les échanges d'informations auxquels elle a donné lieu. La quantité et la qualité des résultats présentés a permis de mettre en évidence la puissance d'analyse que représente le travail en réseau. 2-L'importance de la valorisation des résultats a été soulignée à plusieurs reprises. La diffusion de l'information devrait se faire dans un premier temps par la voie de rapport internes à la DRV, puis par la voie d'articles publiés dans des revues scientifiques. Sur le programme "C. gigas triploïdes", une première diffusion des résultats obtenus en...
Tipo: Text
Palavras-chave: Génétique; Sélection génétique; Huître plate; Ostrea edulis; Génétique population; Croissance.
Ano: 1993
URL: http://archimer.ifremer.fr/doc/00049/15981/13423.pdf

The use of SNPs in characterizing oysters genomes and their resistance to pathogens
Provedor de dados: 5
Autores: Lapegue, Sylvie; Heurtebise, Serge; Harrang, Estelle; Morga, Benjamin; Flahauw, Emilie; Sauvage, Christopher; Boudry, Pierre.
During the last five years, a great effort has been made to develop genomic tools in oyster species all over the world. In Europe, the Pacific cupped oyster, Crassostrea gigas, and the European flat oyster, Ostrea edulis received a special attention as they are the key species for local aquaculture. In particular, the Pacific oyster is a marine bivalve of major economical and ecological importance worldwide. Better knowledge of its physiology and its genetics contributes to optimize its aquaculture production using selective breeding or to understand its invasive behavior in some coastal areas. Current international developments of genomic resources include EST databases, BAC libraries, genome mapping and sequencing. The Genoscope (Evry, France) and the...
Tipo: Text
Palavras-chave: Oysters; Crassostrea gigas; Ostrea edulis; Resistance pathogens; QTLs; Single Nucleotide Polymorphism.
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00015/12584/9454.pdf

Does the prevalence of Bonamia  and Marteilia diseases be reduced on flat oysters (Ostrea edulis) of Atlantic and Mediterranean origin, when they are reared together with the Japanese oyster (Crassostrea gigas) in tidal ponds?
Provedor de dados: 5
Autores: Bodoy, Alain; Bougrier, Serge; Geairon, Philippe; Garnier, Jacqueline; Boulo, Viviane; Heurtebise, Serge.
The flat oyster Ostrea edulis was originally reared in several regions of the European waters. The tidal saline ponds on the Atlantic coasts of France were highly productive before being hit by two consecutive epizooties. Parasitic diseases were described on this species, both of them leading 10 economic losses, as heavy mortalities have usually been recorded before the oysters reach the commercial size. Repeated experiments showed that the two diseases, Marteilia refringens and Bonamia ostreae are still active in this environment. Recently, some evidences were given that haemocytoblasts of Crassostrea gigas had the ability to lyze the Bonamia cells. Therefore, in an experiment of contiguous culture of the two species of oysters, the growth, mortality and...
Tipo: Text
Palavras-chave: Molluscs; Mortalities; Growth; Pathology; Crassostra gigas; Ostrea edulis; Marteilia refringens; Bonamia ostreae.
Ano: 1991
URL: http://archimer.ifremer.fr/doc/1991/acte-3049.pdf

Compte rendu sur la reproduction des huîtres et des moules dans le bassin de Marennes Oléron pour l'année 1985
Provedor de dados: 5
Autores: Ratiskol, Jacqueline; Heurtebise, Serge.
Au cours de l'année 1985, le laboratoire CSRU de La Tremblade a pris en charge le suivi de la reproduction des larves d'huîtres et de moules dans le bassin de Marennes Oléron. Les différents points sont choisis en fonction de critères tels que la proximité des bancs géniteurs (gisements naturels d'huîtres ou de moules) et des zones traditionnelles de reproduction des huîtres : Les Doux et Mérignac pour le bassin, Faulx, Coux et Les Roches pour la Seudre, Les Palles et La mouclière pour la Charente.
Tipo: Text
Palavras-chave: Reproduction; Larves; Crassostrea gigas; Mytilus edulis; Bassin de Marennes Oléron.
Ano: 1986
URL: http://archimer.ifremer.fr/doc/00049/16073/13534.pdf

Phylogéographie des huîtres de mangrove de l'Océan Atlantique Sud : C. gasar et C. rhisophorae
Provedor de dados: 5
Autores: Heurtebise, Serge; Boutet, Isabelle; Verden, B.; Lapegue, Sylvie; Leitao, Alexandra; Thiriot-quievreux, Catherine; Garica, P.; Boudry, Pierre.
Mangrove oysters include various species that are also present on different coasts. In the southern part of the Atlantic Ocean, Crassostrea rhizophorae has been observed along the southern coast of America and Crassostrea gasar along the western coast of Africa. Fifteen populations of these oysters were sampled from these two geographic areas. Their polymorphism was studied on the 16S mitochondrial fragment both by par sequencing and RFLP analysis. Some samples' caryotype were also analysed. Two haplotypes were identified: C. gasar was found in Africa, but also for the first time in South America, whereas C. rhizophorae was only found on the coasts of South America.
Tipo: Text
Palavras-chave: Crassostrea rhizophorae; Crassostrea gasar; Huîtres; Génétique; Phylogéographie.
Ano: 2001
URL: http://archimer.ifremer.fr/doc/2001/acte-3278.pdf

Evidence for the presence of the Portuguese oyster, Crassostrea angulata, in northern China
Provedor de dados: 5
Autores: Lapegue, Sylvie; Batista, Frederico; Heurtebise, Serge; Yu, Ziniu; Boudry, Pierre.
The Pacific oyster, Crassostrea gigas (Thunberg), and the Portuguese oyster, C. angulata (Lamarck), are two closely related taxa. Although these two taxa were both introduced from Asia into Europe. one (C. gigas) was voluntarily introduced in the early 1970s, whereas the other (C. angulata) was presumed to be present in Europe for at least four centuries, but nearly disappeared because of disease. Few C. angulata populations remained in southern Portugal, Spain and Morocco and their putative origin was traced in Taiwan. The present paper reports evidence for its presence in Northern China. We reanalyzed recently published mitochondrial cytochrome oxidase C subunit I (COI) sequence data from presumed Dalianwan oysters (C. talienwhanensis) and compared them...
Tipo: Text
Palavras-chave: Phylogeography; Cytochrome oxidase C subunit 1 gene; Crassostrea talienwhanensis; Gigas; Crosstrea; Crassostrea angulata; Cupped oysters.
Ano: 2004
URL: https://archimer.ifremer.fr/doc/2004/publication-3173.pdf

Ressources génétiques chez l'huître creuse Crassostrea gigas : variabilité, différenciation et adaptation aux conditions de production en Charente Maritime
Provedor de dados: 5
Autores: Huvet, Arnaud; Boudry, Pierre; Baud, Jean-pierre; Nourry, Max; Phelipot, Pascal; Gerard, Andre; Ledu, Christophe; Heurtebise, Serge; Cornette, Florence; Blouin, Frederic; Bedier, Edouard; Lapegue, Sylvie.
es marqueurs génétiques, particulièrement bien adaptés à ces problématiques car hypervariables, ont été mis au point récemment dans notre laboratoire en collaboration avec le laboratoire Génome et Populations de Montpellier (CNRS). Ils permettent désormais l'analyse approfondie et la discrimination fine de populations d'huîtres creuses Crassostrea gigas et de rechercher si certaines d'entre-elles n'auraient pas des caractéristiques intéressantes pour l'ostréiculture locale (croissance, résistance estivale, affinage ... ). La diffusion de telles populations auprès de la profession serait plus rapide car il s'agit de la même espèce. Selon les recommandations du CIEM, il faut effectuer une mise en quarantaine des huîtres importées (après contrôles sanitaires)...
Tipo: Text
Palavras-chave: Génétique; Génétique des populations; Marqueurs génétiques; Microsatellite; Huître creuse; Crassostrea gigas.
Ano: 1998
URL: http://archimer.ifremer.fr/doc/00044/15563/12952.pdf

Acclimatation de nouvelles espèces d'huîtres creuses du genre Crassostrea: hybridations et conservatoire de souches. Contrat de Plan Etat Région Poitou- Charentes 1994-1998 - Convention 95/RPC-R-57 "Génétique" - Rapport année 1995
Provedor de dados: 5
Autores: Boudry, Pierre; Naciri, Yamama; Heurtebise, Serge; Delsert, Claude; Ledu, Christophe; Phelipot, Pascal; Chollet, Bruno; Cochennec, Nathalie; Gerard, Andre.
Le laboratoire IFREMER "Génétique Aquaculture et Pathologie" (GAP) (La Tremblade et Bouin) a entrepris des actions de recherche sur ces différents points. L'Unité de Recherche en Génétique, en collaboration avec tous les membres du Réseau Génétique Mollusques (REGEMO), a axé une partie de ses travaux sur l'acclimatation et l'hybridation de différentes espèces du genre Crassostrea. Ces recherches ont débuté avec le soutien du Conseil Général de Charente-Maritime depuis 1992 sur des essais d'acclimatation de C. virginica, l'huître creuse américaine. En prolongement de cette étude, l'URGE a proposé à la région Poitou-Charentes un programme de recherche plus vaste sur l'ensemble des espèces d'huîtres creuses.
Tipo: Text
Palavras-chave: Génétique; Acclimatation; Hybridation; Crassostrea virgnica.
Ano: 1995
URL: http://archimer.ifremer.fr/doc/00044/15515/12902.pdf

Scanning the genome of the Pacific cupped oyster, Crassostrea gigas, for adaptative differentiation during geographic range expansion
Provedor de dados: 5
Autores: Rohfritsch, Audrey; Bierne, Nicolas; Boudry, Pierre; Heurtebise, Serge; Lapegue, Sylvie.
Originating from the north eastern Asia, Crassostrea gigas has been introduced and translocated, mainly for aquaculture purpose, into several European countries (from Norway to Portugal and in the Mediterranean Sea) (1). Although highly variable, the invasiveness pattern of C. gigas has been demonstrated in several countries and therefore considered as a pest or a noxious species in those areas (2 and 3).Our project aims at identifying the characteristics of such a flourishing species: can its success be explained by chance and/or global warming only or does it exhibit a more important potential of adaptation than other species? Therefore we developed a population genomics approach, known as "genome scan". It corresponds to the study of numerous loci...
Tipo: Text
Palavras-chave: Invasive; AFLP; Genome scan; Crassotrea gigas; Pacific oyster.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/acte-4805.pdf

REGEMO Réseau Génétique Mollusques. Compte rendu de la réunion des 13 et 14 décembre 1993 - Nantes
Provedor de dados: 5
Autores: Gerard, Andre; Naciri, Yamama; Peignon, Jean-marie; Ledu, Christophe; Phelipot, Pascal; Baud, Jean-pierre; Nourry, Max; Haure, Joel; Coatanea, Denis; Oheix, Jocelyne; Vercelli, Catherine; Chabirand, Jean-michel; Borel, Michel; Maurer, Daniele; Heurtebise, Serge; Garnier, Jacqueline; Martin, Anne-genevieve; Tige, Gilbert; Mazurie, Joseph; Langlade, Aime.
Cette réunion des membres du Réseau Génétique Mollusques avait pour objet de faire un bilan provisoire des actions en cours et surtout de mettre en place les protocoles et les calendriers du début de l'année 1994. La première demi-journée a été réservée à un exposé de Yamama NACIRI sur les bases de la Génétique Quantitative et ses applications dans le cadre des programmes de sélection des mollusques.La deuxième demi-journée a été consacrée au bilan des actions de 1993. Les représentants de chaque site, intervenant dans une opération du réseau en 1993, ont fait une présentation orale de leurs résultats. Un résumé de chaque intervention vous est fourni dans ce document. Il est important de souligner que tous ces bilans sont provisoires, en premier lieu parce...
Tipo: Text
Palavras-chave: Génétique; Sélection génétique; Huître plate; Ostrea edulis; Croissance; Génétique population.
Ano: 1993
URL: http://archimer.ifremer.fr/doc/00049/15982/13425.pdf

Etude du niveau d'aneuploïdie dans les populations des zones de captage du bassin de Marennes-Oléron - Contrat Plan Etat Région Poitou-Charentes 1999 - Rapport 1999
Provedor de dados: 5
Autores: Lapegue, Sylvie; Thiriot Quievreux, Catherine; Mccombie, Helen; Heurtebise, Serge; Boudry, Pierre; Robert, Stephane; Soletchnik, Patrick; Goulletquer, Philippe; Gerard, Andre.
Divers travaux, menés depuis 1984, montrent qu'il existe, chez l'huître creuse Crassotrea gigas, des cellules montrant un nombre anormal de chromosomes (2n = 19, 18 ou même 17 au lieu de 2n = 20). Le niveau d'aneuploïdie est déterminé par le décompte des chromosomes à partir de suspensions cellulaires de tissu branchial. Le pourcentage de cellules aneuploïdes est toujours significativement supérieur dans les "lots de queue", c'est-à-dire les huîtres présentant des croissances plus faibles, et peut atteindre plus de 30 %. Récemment, une étude réalisée au sein du programme européen « Genephys » a démontré que plus de 50 % de la variance pour la vitesse de croissance était liée au taux d'aneuploïdie. Le but de l'expérimentation menée en 1999 est de faire un...
Tipo: Text
Palavras-chave: Génétique; Aneuploïdie; Génétique des populations; Biochimie; Marennes-Oléron; Huître creuse; Crassostrea gigas.
Ano: 1999
URL: http://archimer.ifremer.fr/doc/00044/15522/12909.pdf

Ressources génétiques chez les huîtres creuses : conservatoire de souche et marqueurs moléculaires
Provedor de dados: 5
Autores: Boudry, Pierre; Heurtebise, Serge; Collet, Bertrand; Cornette, Florence; Ledu, Christophe; Phelipot, Pascal; Gerard, Andre.
The cupped oyster strain bank was created at Ifremer at La Tremblade in 1992. It was funded by the Poitou-Charentes regional council. Its goals were: 1. to study the intra- and interspecific genetic differentiation within the genus Crassostrea. 2. to study acclimatization potential of foreign oysters in French waters in order to identify species that could replace Crassostrea gigas in case of a major disease outbreak in C. gigas or in case of a need or desire to diversify shellfish production. 3. to study the possibility of interspecific hybridisation in the genus Crassostrea and hybrid performance.
Tipo: Text
Palavras-chave: Saccostrea commercialis; Crassostrea rhisophorae; Crassostrea gasar; Crassostrea sikamea; Crassostrea virginica; Crassostrea rivularis; Crassotea angulata; Crassostrea gigas; Génétique.
Ano: 1997
URL: http://archimer.ifremer.fr/doc/1997/acte-3242.pdf

Contrôle de la gamétogénèse des huîtres creuses et plates. Relations "reproduction" et "génétique"
Provedor de dados: 5
Autores: Gerard, Andre; Naciri-graven, Yamama; Boudry, Pierre; Launey, Sophie; Heurtebise, Serge; Ledu, Christophe; Phelipot, Pascal.
At the end of the 1980s, after two decades of aquaculture research in molluscs, Ifremer initiated its first genetic studies. These new studies quickly revealed the limits of knowledge in aquaculture, particularly in terms of controlling oyster reproduction. This presentation attempts to summarize the available information on genetics in aquaculture. In particular, breeding practices in the 1980s involved populations of broodstock, while genetics requires modern techniques where individuals, not just populations, can be identified. Controlling reproduction at the individual level in oysters raises new questions that existing protocols cannot address, especially in terms of controlling sex determination, individual maturity, spawning synchrony, chemical...
Tipo: Text
Palavras-chave: Gamètes; Fécondation; Induction ponte; Reproducteur; Conditionnement; Gamétogénèse; Génétique; Reproduction; Crassostrea gigas; Huîtres.
Ano: 1995
URL: http://archimer.ifremer.fr/doc/1995/acte-3210.pdf

Programme 3 : Gestion durable des productions ostréicoles : les apports de la génétique. Convention 2000 RPC-A-530 Ifremer - Région Poitou-Charentes
Provedor de dados: 5
Autores: Boudry, Pierre; Huvet, Arnaud; Fabioux, Caroline; Lapegue, Sylvie; Heurtebise, Serge; Ledu, Christophe; Blouin, Frederic; Phelipot, Pascal; Gerard, Andre.
L'étude de la variabilité génétique de l' huître creuse Crassastrea gigas est un point essentiel dans la mise en place de programmes de gestion des ressources génétiques et de sélection chez cette espèce. C. gigas, originaire du Japon, a été introduite dans de nombreuses régions du monde et représente aujourd'hui plus de 95 % de la production mondiale d' huîtres. Introduite en France au début des années 1970 pour remplacer l' huître portugaise Crassostrea angulata, c'est aujourd 'hui une ressource de première importance pour notre région. Malgré la maîtrise du cycle de reproduction de l'espèce et son importance commerciale, elle n' a pas été génétiquement améliorée et on peut donc considérer les stocks comme « sauvages» ou « non domestiqués ». De plus, la...
Tipo: Text
Palavras-chave: Génétique; Ressources génétiques; Différenciation génétique; Hybridation; Anomalies chromosomiques; Huîtres creuses; Crassostrea gigas; Crassostrea angulata.
Ano: 2000
URL: http://archimer.ifremer.fr/doc/00044/15564/12955.pdf