Consulta: creatorFacets:"Hiraoka, Hiroko"
Registros recuperados: 5
Data/hora: 11/06/2024 00:53:14

Detection of DNA of 'Candidatus Mycoplasma haemominutum' and Spiroplasma sp. in unfed ticks collected from vegetation in Japan
Provedor de dados: 19
Autores: Taroura, Shoko; Shimada, Yojiro; Sakata, Yoshimi; Miyama, Takako; Hiraoka, Hiroko; Watanabe, Malaika; Itamoto, Kazuhito; Okuda, Masaru; Inokuma, Hisashi; 猪熊, 壽.
DNA fragments of 'Candidatus Mycoplasma haemominutum', a feline heamobartonella pathogen, were detected from unfed Ixodes ovatus collected from vegetation in Hokkaido, Fukushima and Yamaguchi Prefectures, and unfed Haemaphysalis flava in Yamaguchi Prefecture. This finding suggests that ixodid tick is a possible vector of 'C. Mycoplasma haemominutum'. Spiroplasma DNA was also detected from unfed I. ovatus in Hokkaido, Fukushima and Yamaguchi Prefectures. The analysis of nucleotides sequence suggested that this Spiroplasma was distinct from registered species.
Palavras-chave: Candidatus Mycoplasma haemominutum; Spiroplasma sp; Tick.
Ano: 2005
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/1654

Experimental inoculation of beagle dogs with Ehrlichia species detected from Ixodes ovatus
Provedor de dados: 19
Autores: Watanabe, Malaika; Oikawa, T; Hiraoka, Hiroko; Kaneko, N; Itamoto, Kazuhito; Mizuno, Tohru; Okuda, Masaru; Inokuma, Hisashi; 猪熊, 壽.
Three beagle dogs were inoculated with mice spleen/liver homogenate infected with Ehrlichia species detected from Ixodes ovatus (EIO) and one dog was used as a control. All three infected dogs did not show clinical signs of disease except for mild pyrexia throughout the 41-day study period. Splenomegaly was observed from Day 7 post-inoculation (p.i.) in two of the dogs. Hematological and biochemical abnormalities included mild thrombocytopenia, hypoproteinaemia, hypoalbuminaemia and increased C-reactive protein values. One of the dogs' splenic aspirate sample was PCR-positive for Ehrlichia Day 7 p.i. and another dogs' blood and bone marrow aspirate sample was PCR-positive Day 41 p.i. Sequence analysis of the PCR products showed 100% homology with the...
Palavras-chave: CANINE MONOCYTIC EHRLICHIOSIS; YAMAGUCHI PREFECTURE; INFECTION; JAPAN; DIAGNOSIS; KINETICS; TICKS; PCR.
Ano: 2006
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/65

Detection of Borrelia garinii, Borrelia tanukii and Borrelia sp. closely related to Borrelia valaisiana in Ixodes ticks removed from dogs and cats in Japan.
Provedor de dados: 19
Autores: Hiraoka, Hiroko; Shimada, Yojiro; Sakata, Yoshimi; Watanabe, Malaika; Itamoto, Kazuhito; Okuda, Masaru; Masuzawa, Toshiyuki; Inokuma, Hisashi.
Ticks removed from 1136 dogs and 134 cats all over Japan were examined for Borrelia infection by PCR and sequencing. The 5S-23S rDNA intergenic spacer of Borrelia was detected from two Ixodes persulcatus ticks from two dogs and two unidentified Ixodes spp. from another two dogs in Hokkaido, and two Ixodes granulatus ticks from two cats in Okinawa. Additional 2 I. granulatus from the same cats also showed positive. Sequence analysis of the PCR products revealed that the one from Hokkaido was similar to B. garinii, the three from Hokkaido to B. tanukii, and the four from Okinawa to a novel Borrelia sp. closely related to B. valaisiana. The data was confirmed by analysis of the flagellin gene sequence. Infected ticks carried by companion animals can be...
Ano: 2007
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/743

Detection of Rickettsial DNA in ixodid ticks recovered from dogs and cats in Japan
Provedor de dados: 19
Autores: Hiraoka, Hiroko; Shimada, Yojiro; Sakata, Yoshimi; Watanabe, Malaika; Itamoto, Kazuhito; Okuda, Masaru; Inokuma, Hisashi; 猪熊, 壽.
DNA from ticks recovered from 1137 dogs and 133 cats from Ell over Japan were examined for Rickettsia infection by citrate synthase gene (gltA)-based PCR and partial nucleotide sequencing. A total of 91 dog tick samples and 18 cat tick samples showed a single band of the appropriate size in the nested PCR. Sequence analysis was successfully performed on 102 samples. DNA of Rickettsia japonica or closely related Rickettsia spp. strains were detected from 38 licks in 16 prefectures mainly in western Japan. The other 33, detected from 13 prefectures including Hokkaido and Okinawa, were found to be Rickettsia helvetica or closely related strains. A total of 29 DNA that showed highest homology with Rickettsia akari or closely related strains were detected in 19...
Palavras-chave: Canine; Epidemiology; Feline; Rickettsia; Tick.
Ano: 2005
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/923

Clinical observations of Babesia gibsoni infection with low parasitaemia confirmed by PCR in dogs
Provedor de dados: 19
Autores: Inokuma, Hisashi; Okuda, Masaru; Yoshizaki, Y; Hiraoka, Hiroko; Miyama, Takako; Itamoto, Kazuhito; Une, S; Nakaichi, M; Taura, Y.
http://veterinaryrecord.bvapublications.com/
Palavras-chave: POLYMERASE-CHAIN-REACTION; JAPAN.
Ano: 2005
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/841