Consulta: creatorFacets:"Huang, Bing"
Registros recuperados: 6
Data/hora: 07/06/2024 09:46:50

Réponses physiologiques et biochimiques à une limitation nutritive en phosphore ou en azote sur la réorientation métabolique des lipides polaires chez différentes espèces de microalgues marines
Provedor de dados: 5
Autores: Huang, Bing.
Betaine lipids (BL) are P-free polar lipids, conversely to phospholipids (PL). The metabolic reorientation induced by phosphorus (P) limitation in microalgae producing BL (Tisochrysis lutea and Diacronema lutheri, Haptophyta) or producing low levels of BL (Phaeodactylum tricornutum, Bacillariophyta) was compared to that induced by nitrogen (N) limitation. The carbon destiny and flow in different biosynthetic pathways were studied with a multidisciplinary approach. P or N limitation differently affected carbon metabolism according to microalgal species. P limitation highly decreased photosynthetic activity and respiration of P. tricornutum and T. lutea. Consequently, carbon accumulation was higher than under N limitation in these two species. Both...
Tipo: Text
Palavras-chave: Microalgues marines; Bétaïne lipides; Triacylglycérols; Limitation en phosphore; Lipides polaires; Acides gras oméga-3; Marine microalgae; Betaine lipids; Triacylglycerols; Phosphorus limitation; Polar lipids; Omega- 3 fatty acids.
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00500/61169/64638.pdf

Carbon Orientation in the Diatom Phaeodactylum tricornutum: The Effects of Carbon Limitation and Photon Flux Density
Provedor de dados: 5
Autores: Heydarizadeh, Parisa; Veidi, Brigitte; Huang, Bing; Lukomska, Ewa; Wielgosz-collin, Gaetane; Couzinet-mossion, Aurélie; Bougaran, Gael; Marchand, Justine; Schoefs, Benoit.
Diatoms adapt to changing environmental conditions in very efficient ways. Among the mechanisms that can be activated, the reorientation of carbon metabolism is crucial because it allows the storage of energy into energy-dense molecules, typically lipids. Beside their roles in physiology, lipids are commercially interesting compounds. Therefore studies dealing with this topic are relevant for both basic and applied science. Although the molecular mechanisms involved in the reorientation of carbon metabolism as a response to a deficiency in nutrients such as nitrogen or phosphorus has been partially elucidated, the impacts of carbon availability on the implementation of the reorientation mechanisms remain unclear. Indeed, it has not been determined if the...
Tipo: Text
Palavras-chave: Diatom; Carbon deficiency; Carbon metabolism; Stress; Light intensity; Regulation; Biotechnology; Phosphoenolpyruvate.
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00489/60058/63338.pdf

Betaine lipid and neutral lipid production under nitrogen or phosphorus limitation in the marine microalga Tisochrysis lutea (Haptophyta)
Provedor de dados: 5
Autores: Huang, Bing; Marchand, Justine; Thiriet-rupert, Stanislas; Carrier, Gregory; Saint-jean, Bruno; Lukomska, Ewa; Moreau, Brigitte; Morant-manceau, Annick; Bougaran, Gael; Mimouni, Virginie.
Nitrogen (N) and phosphorus (P) limitations induce neutral lipid accumulation and membrane lipid remodeling in the domesticated oleaginous microalga Tisochrysis lutea. This study compared growth, photosynthetic activity, biochemical and transcriptional responses of T. lutea throughout batch cultures under N or P limitation compared with non-limiting nutrient condition (NP). The results show that, under N limitation, the breakdown and re-use of cellular N-containing compounds contributed to carbohydrates and further triacylglycerol (TAG) accumulation, where the accumulation of docosahexaenoic acid (DHA) in neutral lipids might mainly be derived from the recycling of membrane polar lipids. Conversely, P limitation did not hinder the photosynthetic capacity;...
Tipo: Text
Palavras-chave: Tisochrysis lutea; Phosphorus; Nitrogen; Lipid remodeling; Betaine lipid; Polyunsaturated fatty acid.
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00492/60409/64776.pdf

Nitrogen and phosphorus limitations induce carbon partitioning and membrane lipid remodelling in the marine diatom Phaeodactylum tricornutum
Provedor de dados: 5
Autores: Huang, Bing; Marchand, Justine; Blanckaert, Vincent; Lukomska, Ewa; Ulmann, Lionel; Wielgosz-collin, Gaëtane; Rabesaotra, Vony; Moreau, Brigitte; Bougaran, Gael; Mimouni, Virginie; Morant-manceau, Annick.
Nitrogen (N) and phosphorus (P) limitations induce triacylglycerol (TAG) accumulation and membrane lipid remodelling in the marine diatom Phaeodactylum tricornutum. However, a clear understanding of the metabolic reorientation is still lacking. Carbon partitioning is of great interest because this microalga produces various highly valuable molecules such as lipids and polyunsaturated fatty acids. This study compared growth, photosynthetic activity, biochemical and transcriptional responses of P. tricornutum throughout batch culture under N or P limitation. The integrated results show that the photosynthetic intensity was greatly reduced under N or P limitation. Under N limitation, the degradation and re-use of cellular N-containing compounds contributed to...
Tipo: Text
Palavras-chave: Betaine lipid; Lipid remodelling; Microalga; Nitrogen; Phosphorus; Polyunsaturated fatty acid.
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00491/60249/63861.pdf

Response of CO2-starved diatom Phaeodactylum tricornutum to light intensity transition
Provedor de dados: 5
Autores: Heydarizadeh, Parisa; Boureba, Wafaa; Zahedi, Morteza; Huang, Bing; Moreau, Brigitte; Lukomska, Ewa; Couzinet-mossion, Aurelie; Wielgosz-collin, Gaetane; Martin-jezequel, Veronique; Bougaran, Gael; Marchand, Justine; Schoefs, Benoit.
In this study, we investigated the responses of Phaeodactylum tricornutum cells acclimated to 300 mu mol m(-2) s(-1) photon flux density to an increase (1000 mmol m(-2) s(-1)) or decrease (30 mmol m(-2)s(-1)) in photon flux densities. The light shift occurred abruptly after 5 days of growth and the acclimation to new conditions was followed during the next 6 days at the physiological and molecular levels. The molecular data reflect a rearrangement of carbon metabolism towards the production of phosphoenolpyruvic acid (PEP) and/or pyruvate. These intermediates were used differently by the cell as a function of the photon flux density: under low light, photosynthesis was depressed while respiration was increased. Under high light, lipids and proteins...
Tipo: Text
Palavras-chave: Phaeodactylum tricornutum; Carbon metabolism; Light regulation; Photosynthesis; Chlorophyll fluorescence; Pyruvate hub.
Ano: 2017
URL: http://archimer.ifremer.fr/doc/00395/50620/52341.pdf

Carbon partitioning and lipid remodeling during phosphorus and nitrogen starvation in the marine microalga   Diacronema lutheri   (Haptophyta)
Provedor de dados: 5
Autores: Huang, Bing; Mimouni, Virginie; Lukomska, Ewa; Morant-manceau, Annick; Bougaran, Gael.
The domesticated marine microalga Diacronema lutheri is of great interest for producing various highly valuable molecules like lipids, particularly long‐chain polyunsaturated fatty acids (LC‐PUFA). In this study, we investigated the impact of phosphorus (P) and nitrogen (N) starvation on growth, carbon fixation (photosynthetic activity) and partitioning, and membrane lipid remodeling in this alga during batch culture. Our results show that the photosynthetic machinery was similarly affected by P and N stress. Under N starvation, we observed a much lower photosynthetic rate and biomass productivity. The degradation and re‐use of cellular N‐containing compounds contributed to triacylglycerol (TAG) accumulation. On the other hand, P‐starved cells maintained...
Tipo: Text
Palavras-chave: Carbon partitioning; Diacronema lutheri; Nitrogen; Phosphorus; Polyunsaturated fatty acid.
Ano: 2020
URL: https://archimer.ifremer.fr/doc/00619/73071/72204.pdf