Consulta: creatorFacets:"Hubert, Francoise"
Registros recuperados: 4
Data/hora: 14/06/2024 16:26:20

Genotoxicity and activation of cellular defenses in transplanted zebra mussels Dreissena polymorpha along the Seine river
Provedor de dados: 5
Autores: Chatel, Annelle; Faucet-marquis, Virginie; Gourlay-france, Catherine; Pfohl-leszkowicz, Annie; Hubert, Francoise.
The aim of the present study was to confirm the relevance of studying DNA adduct formation in a field study. In that context, freshwater mussels Dreissena polymorpha, collected in a reference station, were transplanted in different sites with a pollution gradient. After one and two months, mussels were collected and DNA adduct formation was analyzed using the 32P post labelling technique on both gills and digestive glands. In addition, the expression of genes involved in the detoxification system (catalase (CAT), superoxide dismutase (SOD), glutathione S-transferase (GST), HSP70, aryl hydrocarbon receptor (AHR), P glycoprotein (PgP), metallothionein (MT)) was assessed by RT-PCR. DNA adducts were observed at amount comparable to data from literature....
Tipo: Text
Palavras-chave: Biomarker; Pollution; DNA adducts; Detoxification; Dreissena polymorpha; RT-PCR.
Ano: 2015
URL: https://archimer.ifremer.fr/doc/00195/30639/32450.pdf

Rapport d’activités 2018 – Laboratoire Santé Environnement et Microbiologie.
Provedor de dados: 5
Autores: Desdouits, Marion; Garry, Pascal; Gourmelon, Michele; Hervio Heath, Dominique; Hubert, Francoise; Kaelin, Gaelle; Kergaravat, Cedric; Le Mennec, Cecile; Le Guyader, Soizick; Loiseau, Veronique; Lozach, Solen; Maillot, Jessica; Ollivier, Joanna; Parnaudeau, Sylvain; Piquet, Jean-come; Quenot, Emmanuelle; Rocq, Sophie; Schaeffer, Julien; Serghine, Joelle; Vallade, Emilie; Veron, Antoine; Wacrenier, Candice.
Ce rapport présente une synthèse des actions, et travaux réalisés par le laboratoire Santé Environnement et Microbiologie pendant l’année 2018. Les divers projets de recherche sont résumés et les derniers développements ou résultats sont brièvement évoqués. Les actions en tant que Laboratoire National de Référence pour la Microbiologie des Coquillages et les activités pour la coordination du réseau REMI sont également présentées.
Tipo: Text
Palavras-chave: Microbiologie sanitaire; Bactéries entériques; Vibrions; Virus entériques humains; Norovirus; Coquillage; Activité de référence; REMI.
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00491/60218/63584.pdf

Mutagenicity and genotoxicity of suspended particulate matter in the Seine river estuary
Provedor de dados: 5
Autores: Hubert, Francoise; Moisan, Karine; Munschy, Catherine; Tronczynski, Jacek.
Highly mutagenic compounds such as some PAHs have been identified in surface waters and sediments of the Seine river estuary. Suspended particulate matter (SPM) represents a dynamic medium that may contribute to the exposure of aquatic organisms to toxic compounds in the water column of the estuary. In order to investigate major sources of mutagenic contaminants along the estuary, water samples were taken at 25 in downstream of the outlet of an industrial wastewater-treatment plant (WWTP). SPM samples were analyzed for their genotoxicity with two short-term tests, the Salmonella typhimurium mutagenicity assay (TA98 + S9 mix) and the comet assay in the human HepG2 cell line. Sampling sites receiving effluents from a chemical dye industry and WWTP showed the...
Tipo: Text
Palavras-chave: Ames test; Comet assay; Water pollution; Effluent; Suspended particulate matter; Seine estuary.
Ano: 2012
URL: http://archimer.ifremer.fr/doc/00072/18323/16013.pdf

Adsorption of norovirus and ostreid herpesvirus type 1 to polymer membranes for the development of passive samplers
Provedor de dados: 5
Autores: Hubert, Francoise; Morga, Benjamin; Renault, Tristan; Le Guyader, Soizick.
Aims  This study was performed to develop passive sampling methodology for the detection of two viruses in seawater in the area of shellfish production, The Norovirus (NoV), a human pathogen implicated in gastroenteritis outbreaks linked to oyster consumption and the ostreid herpesvirus type 1 (OsHV-1) a virus associated with mass mortalities of Pacific oysters. Methods and Results  Commercially membranes were tested for their capacity to adsorb virus: Zetapor, gauze, nylon, low density polyethylene (LDPE) and Polyvinylidene difluoride (PVDF). Laboratory exposures of membranes to contaminated water samples (stool, sewage, seawater) were performed. Our data shown that the amount of NoV GII genome per membrane measured with qRT-PCR increased with the time of...
Tipo: Text
Palavras-chave: Norovirus; OsHV-1; Pacific oysters; Passive samplers; Seawater; Sewage.
Ano: 2017
URL: http://archimer.ifremer.fr/doc/00365/47646/47670.pdf