Consulta: creatorFacets:"Ikehara, Yuzuru"
Registros recuperados: 4
Data/hora: 11/06/2024 10:55:04

Adjuvant Effect of Oligomannose-Coated Liposome-Based Platform for veccine against African Trypanosomosis
Provedor de dados: 19
Autores: Kuboki, Noritaka; Yokoyama, Naoaki; Namangala, B; Okamura, Masashi; Inoue, Noboru; Takagi, Hideaki; Nakayama, Tomoko; Nishikawa, Yoshifumi; Ikehara, Yuzuru; Kojima, Naoya; 横山, 直明; 井上, 昇; 西川, 義文.
Palavras-chave: Adjuvant; African trypanosome; Immunization; Oligomannose-coated liposome(OML).
Ano: 2008
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2222

Preliminary evaluation of oligomannose-coated liposome vaccines against lethal protozoan infections in mice
Provedor de dados: 19
Autores: Kuboki, Noritaka; Tiwananthagorn, Weerawan; Takagi, Hideaki; Nakayama, Tomoko; Xuan, Xuenan; Inoue, Noboru; Igarashi, Ikuo; Tsujimura, Kunio; Ikehara, Yuzuru; Kojima, Naoya; Yokoyama, Naoaki.
The oligomannose-coated liposome (OML) vaccine is known to induce cellular immunity specific for the encapsulated antigen in immunized mice. In the present study, we preliminarily evaluated the effect of the OML vaccine encapsulating the soluble protozoan lysate of Toxoplasma gondii, Trypanosoma brucei gambiense, or Babesia rodhaini on the corresponding protozoan infections in mice. After the challenge of T. gondii, the OML vaccine group avoided the high mortality resulting from acute infection that was dominantly observed in other control groups. During the infectious course, the development of the T. gondii-specific antibody, which is an indicator of humoral immunity, was constantly controlled at a lower level in the surviving mice of the OML vaccine...
Palavras-chave: Oligomannose-coated liposome (OML); OML; Soluble protozoan antigen; Antibody responses.
Ano: 2007
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/1050

Neospora caninum: Application of apical membrane antigen 1 encapsulated in the oligomannose-coated liposomes for reduction of offspring mortality from infection in BALB/c mice
Provedor de dados: 19
Autores: Zhang, Houshuang; Nishikawa, Yoshifumi; Ikehara, Yuzuru; Kojima, Naoya; Yokoyama, Naoaki; Xuan, Xuenan.
Liposomes coated with neoglycolipids constructed with mannopentaose and  dipalmitoylphosphatidylethanolamine (Man3-DPPE), referred to as M3-DPPE  liposomes, have been shown to induce cellular immunity against antigens encapsulated therein. To evaluate whether these M3-DPPE liposomes have an  adjuvant capacity against Neospora caninum infection, a novel immunization  method utilizing soluble N. caninum apical membrane antigen 1 (NcAMA1)  encapsulated in the M3-DPPE liposomes (M3-NcAMA1) was employed. The  intent was to reduce offspring mortality from N. caninum infection in susceptible,  pregnant BALB/c mice. The results revealed that BALB/c mice developed IgG  antibodies specific to N. caninum. A significant amount of interferon (IFN)-γ  production was...
Palavras-chave: Neospora caninum; Apical membrane antigen 1; Oligomannose-coated liposomes.
Ano: 2010
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2821

Babesia bovis: Subcellular localization of host erythrocyte membrane components during their asexual growth
Provedor de dados: 19
Autores: Okamura, Masashi; Yokoyama, Naoaki; Takabatake, Noriyuki; Okubo, Kazuhiro; Ikehara, Yuzuru; Igarashi, Ikuo; 横山, 直明; 五十嵐, 郁男.
In the present study, the subcellular localization of the host red blood cell (RBC) membrane components, the alpha 2-3-linked sialic acid (SA) residues and the lipid bilayer, was observed during the asexual growth of Babesia bovis using two erythrocyte probes, the SA-specific lectin (MALII) and the lipophilic fluorescent (PKH2) probes, respectively. In confocal laser scanning microscopy with MALII, the SA residues on the surface of parasitized RBCs appeared to accumulate into the intracellular parasites as the parasites matured as well as to remain on the surface of extracellular parasites. Furthermore, when PKH2-labeled RBCs were infected with B. bovis, PKH2 signals were also observed around both the intracellular and the extracellular parasites,...
Palavras-chave: Babesia bovis (B. bouis); SA; MALII; RBC; RT; PBS; Babesia equi (B. equi); PV.
Ano: 2007
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/1040