Consulta: creatorFacets:"Ishikawa,Márcia Mayumi"
Registros recuperados: 8
Data/hora: 06/06/2024 00:59:08

Host-parasite relationship during Epistylis sp. (Ciliophora: Epistylididae) infestation in farmed cichlid and pimelodid fish
Provedor de dados: 86
Autores: Pádua,Santiago Benites de; Martins,Maurício Laterça; Valladão,Gustavo Moraes Ramos; Utz,Laura; Zara,Fernando José; Ishikawa,Márcia Mayumi; Belo,Marco Antonio de Andrade.
Abstract: The objective of this work was to describe the host-Epistylis sp. relationship during infestation on farmed fish. Five Nile tilapia (Oreochromis niloticus) and ten hybrid surubim catfish (Pseudoplatystoma reticulatum x P. corruscans), all diseased, were used for in vivo morphological analysis of sessile peritrichs by contrast microscopy. Fragments of infected tissues were subjected to histological processing and scanning electron microscopy. Epistylis sp. caused hemorrhagic ulcer disease, and cichlids were more prone to develop infestations throughout the body surface due to the attachment of the colonies to the scales, which did not occur with pimelodids. Multifocal granulomatous dermatitis was observed, associated with the hydropic degeneration...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Oreochromis niloticus; Pseudoplatystoma reticulatum; Pseudoplatystoma corruscans; Histopathology; Pathogenesis; Sessile peritrich.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2016000500520

Fauna parasitária dos híbridos siluriformes cachapinta e jundiara nos primeiros estágios de desenvolvimento
Provedor de dados: 86
Autores: Ventura,Arlene Sobrinho; Jerônimo,Gabriela Tomas; Gonçalves,Eduardo Luiz Tavares; Tamporoski,Bianca Rafaela Fiori; Martins,Maurício Laterça; Ishikawa,Márcia Mayumi.
O objetivo deste trabalho foi avaliar a fauna parasitária dos peixes híbridos cachapinta (Pseudoplatystoma corruscans macho x P. reticulatum fêmea) e jundiara (Leiarius marmoratus macho x P. reticulatum fêmea) no período de larvicultura. Um total de 315 animais, 126 híbridos jundiara e 189 híbridos cachapinta, foram examinados de acordo com o estágio de desenvolvimento: larvas, pós-larvas e alevinos. Larvas e pós-larvas foram prensadas entre lâmina e lamínula e observadas sob microscopia óptica. Os alevinos foram observados externamente em estereomicroscópio e necropsiados para avaliação interna dos órgãos. Foram aferidos os parâmetros de qualidade de água em todas as fases de cultivo. O exame parasitológico revelou a presença dos protozoários Epistylis...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Epistylis; Ichthyophthirius multifiliis; Trichodina; Bagre; Larvicultura; Parasitos.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2013000800022

Heparina e K3EDTA como anticoagulantes para tambaqui (Colossoma macropomum Cuvier, 1816)
Provedor de dados: 36
Autores: Pádua,Santiago Benites de; Pilarski,Fabiana; Sakabe,Róberson; Dias-Neto,José; Chagas,Edsandra Campos; Ishikawa,Márcia Mayumi.
Este estudo avaliou a eficácia da heparina sódica e do EDTA tripotássico como anticoagulantes e seus efeitos sobre os parâmetros hematológicos de tambaqui (Colossoma macropomum). Foram utilizados dez indivíduos de tambaqui com médias de 384,9 ± 85,71 g de peso e 27,90 ± 2,10 cm de comprimento total para avaliação da heparina 5.000 UI e 100 UI, bem como do K3EDTA 10%. Foram analisados a inibição da coagulação por 10 h, eritrograma e teste de fragilidade osmótica dos eritrócitos. Os resultados foram submetidos à análise de variância e as médias comparadas pelo teste de Tukey (P &lt; 0,05). Heparina 5000 UI, heparina 100 UI e K3EDTA 10% foram eficazes na prevenção da coagulação por mais de 10 h, no entanto o EDTA tripotássico causou hemólise desde os...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Coagulação sanguínea; Hemólise; Fragilidade osmótica dos eritrócitos; Hematologia de peixes.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672012000200017

Haematological Parameters of the Hybrid Surubim (Pseudoplatystoma reticulatum x P. corruscans) farmed in Brazil
Provedor de dados: 52
Autores: Jerônimo,Gabriela Tomas; Brum,Aline; Pádua,Santiago Benites de; Gonçalves,Eduardo Luiz Tavares; Capecci,Robson Soares; Ishikawa,Márcia Mayumi; Martins,Maurício Laterça.
This study evaluated the haematological parameters of the hybrid surubim (Pseudoplatystoma corruscans x P. reticulatum) farmed in intensive and semi intensive system. A total of 240 fish were examined, 120 from fish farm and 120 from a semi-intensive for comparison between the hot and cold season. The water quality was weekly measured to evaluate the influence of environmental conditions on the haematology. Fish from intensive system showed hematocrit and white blood cell count (WBC) higher in hot season while the total plasmatic protein, hemoglobin concentration, mean corpuscular hemoglobin concentration (MCHC) and mean corpuscular volume (MCV) were higher in cold season. In fish from semi intensive system, the red blood cells count (RBC) and total...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Culture system; Hematology; Seasonality; Siluriformes.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000200254

Borrelia sp. in naturally infected Didelphis aurita (Wied, 1826) (marsupialia: didelphidae)
Provedor de dados: 52
Autores: Abel,Isis dos Santos; Almeida Junior,Denclair Escobar de; Fonseca,Adivaldo Henrique da; Soares,Cleber Oliveira; Ishikawa,Márcia Mayumi.
Fifty-six opossums (Didelphis aurita) were captured on the campus of Universidade Federal Rural do Rio de Janeiro, Seropédica county, Rio de Janeiro state, in order to investigate the occurrence of Borrelia sp among them in relation with the study of spirochaetemia and its ectoparasites. Blood tests were made through dark field and phase contrast microscopy, as well as the obtainment of blood smears. Smears were stained with Giemsa stain, which did not prove efficacy. There was no relation between results obtained through blood tests (13 opossum positive for Borreliasp.), and this technique (two positive animals). Parasitaemia studies of 37 animals kept in captivity as well as of several recaptures in which animals once negative proved to be positive days...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Borrelia sp.; Didelphis aurita; Marsupial; Amblyomma cajennense; Ctenocephalides sp.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000300010

Evaluation of antibodies production against Borrelia burgdorferi in cattle submitted to rBm86 protein Boophilus microplus tick immunization and associated challenges influence
Provedor de dados: 58
Autores: Ishikawa,Márcia Mayumi; Fonseca,Adivaldo Henrique; Yoshinari,Natalino Hajime; Osório,Ana Luiza Alves Rosa.
IgG antibodies production against Borrelia burgdorferi in immunized cattle with rBm86 protein from Boophilus microplus was evaluated as well as the influence of the association between immunizations and stress through indirect ELISA test during one year. In the present study there was no influence of the isolated challenged used on the production of IgG antibodies against B. burgdorferi. The rBm86 immunogen did not cause significant oscillation in the production of IgG antibodies against B. burgdorferi capable to interfere in the serological results for Lyme Borreliosis in cattle. This study demonstrated the possibility of transitory changes in the production of antibodies after the association of vaccine stimuli and stress, emphasizing the necessity of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antibodies; Borrelia; Cattle; Tick.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500032

Heparina e Na2EDTA como anticoagulantes para surubim híbrido (Pseudoplatystoma reticulatum x P. corruscans): eficácia e alterações hematológicas
Provedor de dados: 65
Autores: Ishikawa,Márcia Mayumi; Pádua,Santiago Benites de; Satake,Fabiana; Hisano,Hamilton; Jerônimo,Gabriela Tomas; Martins,Maurício Laterça.
Este estudo avaliou o efeito da heparina 100UI e do Na2EDTA nas concentrações de 3, 5 e 10% sobre a coagulação sanguínea e os parâmetros hematológicos de surubim híbrido. Foram utilizados 10 peixes, com peso médio de 386,7±24,3g e comprimento total médio de 38,7±6,4cm, para colheita das amostras sanguíneas e determinação do percentual do hematócrito, teor de proteínas plasmáticas totais e teste de fragilidade osmótica dos eritrócitos (FOE). Os dados foram submetidos à análise de variância, e as médias foram comparadas pelo teste de Tukey, a 5% de probabilidade. A coagulação foi eficientemente inibida quando utilizado o Na2EDTA; já as amostras com heparina coagularam 10 horas após a coleta. Houve aumento na fragilidade osmótica dos eritrócitos (P&lt;0,01),...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anticoagulante; Coagulação; Hematologia de peixes; Pseudoplatystoma sp.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782010000700012

Histopathology from liver of tuvira ( Gymnotus spp.) parasitized by larvae of nematodes
Provedor de dados: 65
Autores: Ventura,Arlene Sobrinho; Ishikawa,Márcia Mayumi; Gabriel,Andrea Maria de Araújo; Silbiger,Helcy Lylian Nogueira; Cavichiolo,Fabiana; Takemoto,Ricardo Massato.
ABSTRACT: The aim of this study was to evaluate the histological changes in the liver of thirty-five Gymnotusspp. parasitized by endohelminths collected between April 2012 to October 2013 in commercial bait fish farming of Pantanal basin. Histological cuts of 7µm were stained with hematoxylin-eosin for parasites research and liver changes and have also been submitted to the Perls histochemical method for evaluation of hemosiderosis (Fe+++) based on the incidence degree and severity of change (Grade I, II and III) and tests for the presence of central melanomacrophages. Parasites identified were: Brevimulticaecumsp. with a prevalence of 22,9%, Eustrongylidessp 17,1%, Contracaecumtype I 68,7%, Contracaecumtype II 5,7%, Contracaecumtype III 5,7% and larvae of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hepatic histopathology; Parasitology; Commercial bait fish farming; Anisakidae; Gymnotusspp.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000701233