Consulta: creatorFacets:"Jaouen, Pascal"
Registros recuperados: 13
Data/hora: 11/06/2024 09:19:31

Fractionation of fish protein hydrolysates by ultrafiltration and nanofiltration: impact on peptidic populations
Provedor de dados: 5
Autores: Bourseau, Patrick; Vandanjon, Laurent; Jaouen, Pascal; Chaplain-derouiniot, Maryse; Masse, Anthony; Guerard, Fabienne; Chabeaud, Aurélie; Fouchereau-peron, Martine; Le Gal, Yves; Ravallec-ple, Rosenn; Berge, Jean-pascal; Picot, Laurent; Piot, Jean -marie; Batista, Ireneu; Thorkelsson, Gudjon; Delannoy, Charles; Jakobsen, Greta; Johansson, Inger.
The production by enzymatic treatment of fish protein hydrolysates (FPH) is a promising route to add value to fisheries proteinic co-products (fish frames, heads etc.). Indeed, FPH possess good nutritional properties and biological activities for food and feed uses. Pressure-driven membrane separations such as ultrafiltration (UF) and nanofiltration (NF) can be used after the hydrolysis to, increase the specific activities of the FPH. This paper discusses the impact of a two-step UF/NF process producing four different fractions on two industrial FPH with different hydrolysis degrees. Fractionation is carried out in "realistic" conditions for an industrial process, on highly concentrated FPH solutions (about 100 g of dry matter/L) at a high volume reduction...
Tipo: Text
Palavras-chave: Peptidic profile; Fractionation process; Membrane separation; Nanofiltration; Ultrafiltration; FPH; Fish protein hydrolysate.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/2009/publication-6653.pdf

Aromas potentiality of tuna cooking juice concentrated by nanofiltration
Provedor de dados: 5
Autores: Walha, Khaled; Ben Amar, Raja; Masse, Anthony; Bourseau, Patrick; Cardinal, Mireille; Cornet, Josiane; Prost, Carole; Jaouen, Pascal.
Tuna cooking juices contain high organic load preventing the rejection in the environment without treatment. But the effluents present an interesting fishy odour and it is worth recovering aroma compounds. In this work, two industrial tuna cooking juices were concentrated by nanofiltration. Nanofiltration performance was discussed in terms of permeation fluxes, organic matter retention and impact on the aromatic properties of juices. NF sharply decreases the global intensity of juices and modifies their aromatic equilibrium. However, the main characteristics and the marine nature of juices were kept. A pre-treatment by microfiltration (MF) induces a marked increase in permeation fluxes during NF concentration while it slightly affects the aromatic...
Tipo: Text
Palavras-chave: Tuna cooking juice; Aromas concentration; Membrane separation; Nanofiltration; Sensory analysis.
Ano: 2011
URL: http://archimer.ifremer.fr/doc/00016/12711/9689.pdf

Influence of hydrolysis time on protein recovery and amino acid composition of hydrolysates from Sepia officinalis viscera
Provedor de dados: 5
Autores: Soufi-kechaou, Emna; Jaouen, Pascal; Ben Amar, Raja; Berge, Jean-pascal.
Enzymatic hydrolysis of cuttlefish viscera by Protamex, Pepsin, Delvolase and Multifect Neutral was investigated in a batch reactor during 6 hours with an hourly sampling. The influence of the enzyme used and the hydrolysis conditions (operating pH, temperature) was studied with regards to the extent of proteolytic degradation and to molecular weight distribution of the peptides. After 360 min of hydrolysis, cuttlefish viscera treated with Pepsin yielded significantly (p &lt; 0.05) higher protein recoveries compared to the substrate autolysed or treated with other external proteases. However, the amino acid composition showed that this was Protamex hydrolysis which gave the highest percentage of essential amino acids. At least, according to the FPLC...
Tipo: Text
Palavras-chave: Enzymatic hydrolysis; Cuttlefish Protein Hydrolysates (CPH); Cuttlefish By-Products (CBP); Essential Amino Acids (EAA); Protein recovery.
Ano: 2012
URL: http://archimer.ifremer.fr/doc/00114/22508/20191.pdf

Performances of dead-end ultrafiltration of seawater: from the filtration and backwash efficiencies to the membrane fouling mechanisms
Provedor de dados: 5
Autores: Masse, Anthony; Arab, Oumnia; Sechet, Veronique; Jaouen, Pascal; Pontie, Maxime; Sabiri, Nour-eddine; Plantier, Severine.
The present work investigates the fouling mechanisms of PVDF hollow fibre membrane (0.03 μm) during the dead end ultrafiltration at a fixed permeate flux (outside to inside configuration) of complex synthetic seawater composed by humic acids, alginic acids, inorganic particles and numerous salts at high concentrations. Short term ultrafiltration experiments at 100 L.h-1.m-2 show that the optimal specific filtered volume seems to be equal to 50 L.m-2. A residual fouling resistance equal to 2.1010 m-1 is added after each cycle of filtration during 8h of ultrafiltration at 100 L.h-1.m-2 and 50 L.m-2. Most of the fouling is reversible (80%). Organics are barely (15% of humic acids) retained by the membrane. Backwash efficiency drops during operation which...
Tipo: Text
Palavras-chave: Seawater; Ultrafiltration; Fouling membrane; Alginic acids; Humic acids.
Ano: 2015
URL: http://archimer.ifremer.fr/doc/00286/39716/38172.pdf

Mild processing techniques and development of functional marine protein and peptide ingredients
Provedor de dados: 5
Autores: Thorkelsson, G; Sigurgisladottir, S; Geirsdottir, M; Jóhannsson, R; Guerard, Fabienne; Chabeaud, Aurelie; Bourseau, Patrick; Vandanjon, Laurent; Jaouen, Pascal; Chaplain-derouiniot, M; Fouchereau-peron, Martine; Martinez Alvarez, Oscar; Le Gal, Yves; Ravallec-ple, Rozenn; Picot, Laurent; Berge, Jean-pascal; Delannoy, Charles; Jakobsen, Greta; Johansson, I; Batista, Ireneu.
Tipo: Text
Ano: 2008
URL: http://archimer.ifremer.fr/doc/00056/16747/14184.pdf

Optimization of hydrolysis of sardine (Sardina pilchardus) heads with Protamex: enhancement of lipid and phospholipid extraction
Provedor de dados: 5
Autores: Dumay, Justine; Allery, Marion; Donnay-moreno, Claire; Barnathan, Gilles; Jaouen, Pascal; Carbonneau, Marie Elise; Berge, Jean-pascal.
BACKGROUND: Fish by-products are not considered as valuable raw materials even if they usually contain valuable components such as lipids. Fish lipids are well known for their nutritional potential and health effects but their extraction remains problematic due to the use of organic solvents. Enzymatic hydrolysis such as the proteolysis of tissues can lead to lipid extraction. RESULTS: Hydrolysis of sardine heads by Protamex was studied (temperature, hydrolysis time and enzyme-substrate ratio) using response surface methodology in order to obtain the highest release of lipids and particularly phospholipids. No relation between the degree of hydrolysis and lipid recovery were depicted; however, optimum conditions for both the release of lipids and...
Tipo: Text
Palavras-chave: Response surface methodology; Proteolysis; Sardine; By product; Fatty acids; Phospholipids.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/2009/publication-6651.pdf

Impact of granular filtration on ultrafiltration membrane performance as pre-treatment to seawater desalination in presence of algal blooms
Provedor de dados: 5
Autores: Sabiri, Nour-eddine; Sechet, Veronique; Jaouen, Pascal; Pontie, Maxime; Masse, Anthony; Plantier, Severine.
To mitigate fouling of the ultrafiltration (UF) membrane and improve permeate quality, we coupled granular filters (GF) with UF membrane as a pre-treatment for reconstituted seawater in the presence of algal bloom. Mono and bilayer granular filtrations were led at a mean velocity of 10 m h(-1) over a 7-hour period. Both GF gave the same algal cell retention rate (similar to 63%) after 7 hours of filtration. Turbidity reduction rate was 50% for the monolayer filter and 75% for the bilayer filter. Resulting organic matter removal rate was 10% for the monolayer filter and 35% for the bilayer filter. Dissolved organic carbon removal was low (20%) with the bilayer filter and non-existent with the monolayer filter. GF-coupled UF reduced humic acids in the...
Tipo: Text
Palavras-chave: Algal bloom; Fouling; Granular filtration; Pre-treatment; Seawater; Ultrafiltration.
Ano: 2018
URL: http://archimer.ifremer.fr/doc/00442/55315/56845.pdf

Les Biotechnologies Marines dans le Grand Ouest
Provedor de dados: 5
Autores: Boyen, Catherine; Jaouen, Pascal; Blanchard, Gilbert; Compere, Chantal; Dufour, Alain; Durand, Patrick; Guerard, Fabienne; Hallouin, Florence; Jebbar, Mohamed; Le Blay, Gwenaelle; Le Deit, Hervé; Le Seyec, Jocelyne; Monks, Brian; Portal-sellin, Rachel; Probert, Ian; Pruvost, Jeremy.
Marine (= blue) biotechnology, i.e. the utilization of marine bio-resources as a target or source of biotechnological applications, is a field with massive potential for innovation and economic growth. In a context of rapid climate change and increasing pressure on natural resources, renewed interest in marine biotechnology has been promoted by application of recent methodological and technological advances, notably in bioprocessing and in the various –omics domains, to the study of marine biodiversity. Marine biological resources potentially represent a sustainable raw material for exploitation in diverse fields, including nutrition, health, agriculture, aquaculture, energy, environment, and cosmetics. Marine biotechnology is now recognized as a...
Tipo: Text
Ano: 2015
URL: http://archimer.ifremer.fr/doc/00273/38460/36860.pdf

Enzymatic hydrolysis combined to membranes for upgrading seafood by-products
Provedor de dados: 5
Autores: Bourseau, Patrick; Chabeaud, Aurélie; Vandanjon, Laurent; Masse, Anthony; Jaouen, Pascal; Fleurence, Joël; Berge, Jean-pascal.
This review reports some interesting works on the recovery and fractionation by membrane techniques of marine compounds resulting from hydrolysis of fish by-products such as proteins or peptides, lipids or fatty acids, and carbohydrates. Ultrafiltration (UF) appears as the most widely used technique for such applications. It must be noted that only a few works deal with the optimization or the enhancement of processes for the recovery of added value molecules. Indeed, most of works use UF as a convenient and simple tool for solute fractionation, for this reason the influence of operating parameters on membrane separation efficiency is unfortunately seldom considered. When attention is paid in a study to the process development, it is specified in this...
Tipo: Text
Ano: 2009
URL: http://archimer.ifremer.fr/doc/2009/publication-7408.pdf

Enzymatic hydrolysis of cuttlefish (Sepia officinalis) and sardine (Sardina pilchardus) viscera using commercial proteases: Effects on lipid distribution and amino acid composition
Provedor de dados: 5
Autores: Kechaou, Emna; Durnay, Justine; Donnay-moreno, Claire; Jaouen, Pascal; Gouygou, Jean-paul; Berge, Jean-pascal; Ben Arnar, Raja.
Total lipid and phospholipid recovery as well as amino acid quality and composition from cuttlefish (Sepia officinalis) and sardine (Sardina pilchardus) were compared. Enzymatic hydrolyses were performed using the three proteases Protamex, Alcalase, and Flavourzyme by the pH-stat method (24 h, pH 8, 50 degrees C). Three fractions were generated: an insoluble sludge, a soluble aqueous phase, and an oily phase. For each fraction, lipids, phospholipids, and proteins were quantified. Quantitative and qualitative analyses of the raw material and hydrolysates were performed. The degree of hydrolysis (DH) for cuttlefish viscera was 3.2% using Protamex, 6.8% using Flavourzyme, and 7% using Alcalase. DH for sardine viscera was 1.9% (using Flavourzyme), 3.1% (using...
Tipo: Text
Palavras-chave: Sardine; Viscera; Proteolysis; Marine lipids; Cuttlefish; By products.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/2009/publication-6366.pdf

Impact of ultrafiltration and nanofiltration of an industrial fish protein hydrolysate on its bioactive properties
Provedor de dados: 5
Autores: Picot, Laurent; Ravallec, Rozenn; Fouchereau-peron, Martine; Vandanjon, Laurent; Jaouen, Pascal; Chaplain-derouiniot, Maryse; Guerard, Fabienne; Chabeaud, Aurelie; Legal, Yves; Martinez Alvarez, Oscar; Berge, Jean-pascal; Piot, Jean-marie; Batista, Irineu; Pires, Carla; Thorkelsson, Gudjon; Delannoy, Charles; Jakobsen, Greta; Johansson, Inger; Bourseau, Patrick.
BACKGROUND: Numerous studies have demonstrated that in vitro controlled enzymatic hydrolysis of fish and shellfish proteins leads to bioactive peptides. Ultrafiltration (UF) and/or nanofiltration (NF) can be used to refine hydrolysates and also to fractionate them in order to obtain a peptide population enriched in selected sizes. This study was designed to highlight the impact of controlled UF and NF on the stability of biological activities of an industrial fish protein hydrolysate (FPH) and to understand whether fractionation could improve its content in bioactive peptides. RESULTS: The starting fish protein hydrolysate exhibited a balanced amino acid composition, a reproducible molecular weight (MW) profile, and a low sodium chloride content, allowing...
Tipo: Text
Palavras-chave: Fish protein hydrolysate; Ultrafiltration; Nanofiltration; Membrane separation; Fractionation process; Bioactive peptide.
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00011/12217/9259.pdf

Optimization of common cuttlefish (Sepia officinalis) protein hydrolysate using Pepsin by Response Surface Methodology
Provedor de dados: 5
Autores: Kechaou, Emna; Berge, Jean-pascal; Jaouen, Pascal; Ben Amar, Raja.
Protein hydrolysate was prepared from common cuttlefish Sepia officinalis by Pepsin. Hydrolysis conditions (time, temperature, and enzyme activity) were optimized by response surface methodology (RSM) using a factorial design. The regression coefficient was close to 0.999, observed during both experimental and validation runs, and indicated the validity of prediction model. An enzyme activity to substrate ratio (X1), 0.04 AU/g protein; time (X2), 85 minutes; and temperature (X3), 45 °C were found to be the optimum conditions for a higher degree of hydrolysis (21%) and nitrogen recovery (90%) using Pepsin. According to amino acid analysis results and chemical score, methionine and histidine are the limiting amino acids in the hydrolysates, in that order....
Tipo: Text
Palavras-chave: Cuttlefish protein hydrolysate (CPH); Amino acid; Enzyme; Sepia officinalis.
Ano: 2015
URL: http://archimer.ifremer.fr/doc/00182/29285/27715.pdf

Recovery of valuable soluble compounds from washing waters generated during small fatty pelagic surimi processing by membrane processes
Provedor de dados: 5
Autores: Dumay, Justine; Radier, Severine; Barnathan, Gilles; Berge, Jean-pascal; Jaouen, Pascal.
This work focuses on the treatment of washing waters coming from surimi manufacturing using ultrafiltration technology at a laboratory scale. Four membrane materials (poly-ether sulfone, polyacrilonytrile, poly vinylidene fluoride and regenerated cellulose) and 5 Molecular Weight Cut-Off (from 3 to 100 kDa) have been studied at bench laboratory scale using the pilot Rayflow (R) 100, commercialised by Rhodia Orelis. The investigation deals with the ability for membranes to offer a high retention of biochemical compounds (proteins and lipids). Results obtained during adsorption tests showed that the regenerated cellulose material seems to be the most appropriate with regards to pore size reduction due to the protein-adsorption. During the ultrafiltration of...
Tipo: Text
Palavras-chave: Fish oil; Regenerated cellulose; Surimi wastewater; Enzymatic hydrolysis; Ultrafiltration.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/publication-4359.pdf