Consulta: creatorFacets:"Joaquim, Sandra"
Registros recuperados: 6
Data/hora: 09/06/2024 17:07:51

A Microarray-Based Analysis of Gametogenesis in Two Portuguese Populations of the European Clam Ruditapes decussatus
Provedor de dados: 5
Autores: Sousa, Joana; Milan, Massimo; Bargelloni, Luca; Pauletto, Marianna; Matias, Domitilia; Joaquim, Sandra; Matias, Ana Margarete; Quillien, Virgile; Leitao, Alexandra; Huvet, Arnaud.
The European clam, Ruditapes decussatus is a species with a high commercial importance in Portugal and other Southern European countries. Its production is almost exclusively based on natural recruitment, which is subject to high annual fluctuations. Increased knowledge of the natural reproductive cycle of R. decussatus and its molecular mechanisms would be particularly important in providing new highly valuable genomic information for better understanding the regulation of reproduction in this economically important aquaculture species. In this study, the transcriptomic bases of R. decussatus reproduction have been analysed using a custom oligonucleotide microarray representing 51,678 assembled contigs. Microarray analyses were performed in four gonadal...
Tipo: Text
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00188/29935/28623.pdf

A microarray-based analysis of oocyte quality in the European clam Ruditapes decussatus
Provedor de dados: 5
Autores: De Sousa, Joana Teixeira; Milan, Massimo; Pauletto, Marianna; Bargelloni, Luca; Joaquim, Sandra; Matias, Domitilia; Matias, Ana Margarete; Quillien, Virgile; Leitao, Alexandra; Huvet, Arnaud.
A microarray-based analysis was performed with the objective of describing genomic features of oocytes and looking for potential markers of oocyte quality in the economically important European clam, Ruditapes decussatus. Oocytes of 25 females from Ria de Aveiro, Western coast of Portugal (40°42′N; 08°40′W) were selected for this study and oocyte quality was estimated by success of D-larval rate under controlled conditions, which appeared to vary from 0 to 95 %. By genome-wide expression profiling with a DNA microarray, 526 probes appeared differentially expressed between two groups representing the largest and smallest value of D larval rates, named good (represented by a mean D-larval yield of 57 ± 22%) and poor (9 ± 5%) quality oocytes. Enrichment...
Tipo: Text
Palavras-chave: Ruditapes decussatus; Oocyte; Developmental success; Gene expression; Marine bivalve; Transcriptomics.
Ano: 2015
URL: https://archimer.ifremer.fr/doc/00260/37147/35676.pdf

Oocyte and embryo quality in Crassostrea gigas (Portuguese strain) during a spawning period in Algarve, South Portugal
Provedor de dados: 5
Autores: Massapina, Clara; Joaquim, Sandra; Matias, Domitilia; Devauchelle, Nicole.
Egg quality is one of the most important factors determining larval viability. The study of oocytes released by 'wild' broodstock will contribute to the definition of quality criteria applied to hatcheries as well as being an indicator of the oyster reproduction potential and aquaculture ongrowing systems. The aim of this study was to determine the relationship between condition of Crassostrea gigas spp. angulata (Portuguese strain) from 'wild' broodstock (condition index, gonadal maturity and number of oocyte released), oocyte biochemistry and hatching rate of eggs during the spring/summer of 1995. Stages of gonadal maturity and condition index indicated that natural spawning of this species occurred between April and August with the highest spawning...
Tipo: Text
Palavras-chave: Crassostrea gigas; Portuguese strain; Condition index; Gonadal maturity; Oocyte biochemical composition; Larval viability.
Ano: 1999
URL: http://archimer.ifremer.fr/doc/00043/15442/12814.pdf

First study in cryopreserved Crassostrea angulata sperm
Provedor de dados: 5
Autores: Riesco, Marta F.; Felix, Francisca; Matias, Domitilia; Joaquim, Sandra; Suquet, Marc; Cabrita, Elsa.
Sperm cryopreservation is a widely employed technique that promotes alternative techniques to contribute to broodstock management or restoration programs for species of commercial interest, endangered species or species with an interesting genotype. The preservation of genetic material from improved stocks or from the original population is extremely important for the oyster aquaculture industry to prevent the potential impacts of epidemic diseases and natural disasters. The Portuguese oyster, Crassostrea angulata, was the most important species commercialized by the shellfish industry. However, inadequate management of this industry and pathology occurrences resulted in a significant decrease in natural populations. For this reason, in this work a...
Tipo: Text
Palavras-chave: Crassostrea angulata; Sperm; Cryopreservation; Motility; Viability; Fertilization.
Ano: 2017
URL: http://archimer.ifremer.fr/doc/00333/44432/44098.pdf

The reproductive cycle of the European clam Ruditapes decussatus (L., 1758) in two Portuguese populations: Implications for management and aquaculture programs
Provedor de dados: 5
Autores: Matias, Domitilia; Joaquim, Sandra; Matias, Ana Margarete; Moura, Paula; Sousa, Joana; Sobral, Paula; Leitao, Alexandra.
The reproductive cycle, as well as its nutrient storage and utilization, of two populations of Ruditapes decussatus from the main production areas of this species: Ria de Aveiro and Ria Formosa Lagoon (Portugal) were studied here over a 24 month period (May 2010-April 2012). The reproductive cycle of both populations of R. decussatus followed an annual cyclicality that comprised a ripe stage in spring followed by a spawning period that began in late spring and extended throughout summer until early autumn. This extended and continuous spawning period may be an advantageous strategy for this species by ensuring a continuous supply of gametes. Moreover, R. decussatus can adopt different reproductive strategies depending on the geographical origin. The...
Tipo: Text
Palavras-chave: European clam; Ruditapes decussatus; Reproductive cycle; Portuguese populations; Biochemical composition; Seasonal variations.
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00152/26292/25461.pdf

Insights into Molecular Features of Venerupis decussata Oocytes: A Microarray-Based Study
Provedor de dados: 5
Autores: Pauletto, Marianna; Milan, Massimo; Teixeira De Sousa, Joana; Huvet, Arnaud; Joaquim, Sandra; Matias, Domitilia; Leitao, Alexandra; Patarnello, Tomaso; Bargelloni, Luca.
The production of Venerupis decussata relies on wild seed collection, which has been recently compromised due to recruitment failure and severe mortalities. To address this issue and provide an alternative source of seed, artificial spawning and larval rearing programs were developed. However, hatchery-based seed production is a relatively new industry and it is still underdeveloped. A major hurdle in the European clam seed production is the control of spawning and reproduction, which is further hindered by the impossibility of obtaining fertile gametes by gonadal "stripping'', as meiosis re-initiation is constrained to a maturation process along the genital ducts. In the present study, oocytes were collected from 15 females and microarray analyses was...
Tipo: Text
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00254/36532/35059.pdf