Consulta: creatorFacets:"Justyna Witkowicz"
Registros recuperados: 1
Data/hora: 16/06/2024 03:29:28

12x coverage 454/Sanger Hybrid Assembly of Cucumber (Cucumis sativus L. cv.Borszczagovski) genome - the most efficient way to begin post genomic era
Provedor de dados: 17
Autores: Rafa&amp;#x142; W&amp;#xf3;ycicki; Justyna Witkowicz; Magdalena Pawe&amp;#x142;kowicz; Ewa Siedlecka; Wojciech Gutman; Wojciech Pl&amp;#x105;der; Anna Seroczy&amp;#x144;ska; Mieczys&amp;#x142;aw &amp;#x15a;miech; Katarzyna Niemirowicz-Szczytt; Stanis&amp;#x142;aw Karpi&amp;#x144;ski; Stefan Malepszy; Zbigniew Przybecki.
New sequencing technologies make it possible to achieve genome sequence fast and cheap. Since the assembly step of such next generation reads is still not well standardized it is the most cumbersome part of sequencing projects. &amp;#xd;&amp;#xa;We present here approaches undertaken on the way to the draft assembly the cucumber (Cucumis sativus L. cv. Borszczagowski) genome done with the use of 8x unpaired and 4x paired (3 Kbp) pyrosequenced 454 XLR Titanium reads, together BAC library ends fragments (12,7x physical coverage). Two different assembly approaches, namely Celera and Celera/Arachne were finally used. The Celera result was 15&amp;#x2019;196 contigs of 197 Mbp length and N50 27&amp;#x2019;086 bp and 4&amp;#x2019;157 scaffolds of 224 Mbp length...
Tipo: Poster
Palavras-chave: Biotechnology; Genetics &amp; Genomics; Bioinformatics; Plant Biology.
Ano: 2010
URL: http://precedings.nature.com/documents/5466/version/1