Consulta: creatorFacets:"Kaushik, Sadasivam"
Registros recuperados: 40
Data/hora: 10/06/2024 15:13:26

Fat deposition and flesh quality in seawater reared, triploid brown trout (Salmo trutta) as affected by dietary fat levels and starvation
Provedor de dados: 5
Autores: Regost, Christelle; Arzel, Jacqueline; Cardinal, Mireille; Laroche, M; Kaushik, Sadasivam.
Three isoproteic (crude protein content: 56%) diets with different fat levels (11%, 20%, and 26%) were fed to triplicate groups of triploid brown trout (initial average body weight of 1.5 kg), reared in seawater. At the end of 3 months of feeding, fish fed the high-fat (HF) diet were split into two groups: a triplicate group of fish received the low-fat diet and another triplicate group was kept unfed for a further 2-month period. Fish initially fed the low-fat diet during the first period were continued to be fed the same diet. Fish fed the medium-fat (MF) diet during period 1 were eliminated for period 2. At the end of each period, comparative whole body analyses, sensory and instrumental (texture and colour) analyses were made on fresh and smoked...
Tipo: Text
Palavras-chave: Texture; Colour; Sensory analyses; Starvation fat deposition; Dietary fat; Salmo trutta.
Ano: 2001
URL: http://archimer.ifremer.fr/doc/2001/publication-377.pdf

Protein sources in feed for farmed fish
Provedor de dados: 5
Autores: Medale, Francoise; Kaushik, Sadasivam.
Fish require diets containing 30 to 55% of crude protein and an amino acid supply precisely adapted to meeting the needs for optimal growth. Fish meal is an ideal protein source for aquafeed because it has a high protein level (65% to 72%) with an ideal proportion of all ten indispensable amino acids that meet the requirements of all fish species. However, finding alternatives to fish meal has become an absolute necessity in order to reduce the reliance of aquaculture on marine ingredients. Animal by-product meals (meat meal, bone meal, blood meal, poultry by-product meal, hydrolysed feather meal) are used as alternative protein sources in aquafeed in extra-European countries. Since the use of animal protein in aquafeed is forbidden in Europe (except non...
Tipo: Text
Palavras-chave: Plant proteins; Fish meal; Fish feeding; Antinutritionnal factors; Amino acids.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/2009/publication-6556.pdf

Genotype by diet interactions in European sea bass (Dicentrarchus labrax L.): Nutritional challenge with totally plant-based diets
Provedor de dados: 5
Autores: Le Boucher, Richard; Vandeputte, Marc; Dupont-nivet, M.; Quillet, E.; Ruelle, Francois; Vergnet, Alain; Kaushik, Sadasivam; Allamellou, J. M.; Medale, F.; Chatain, Beatrice.
Aquaculture of carnivorous species has strongly relied on fish meal and fish oil for feed formulation; however, greater replacement by terrestrial plant-based products is occurring now. This rapid change in dietary environment has been a major revolution and has to be taken into consideration in breeding programs. The present study analyzes potential consequences of this nutritional tendency for selective breeding by estimating genetic parameters of BW and growth rates estimated by the thermal growth coefficient (TGC) over different periods with extremely different diets. European sea bass (Dicentrarchus labrax L.) from a factorial cross (1,526 fish) between 25 sires and 9 dams were used to estimate heritabilities and genotype by diet interaction. Starting...
Tipo: Text
Palavras-chave: European sea bass; Genetic correlation; Genotype by diet interaction; Genotype by environment interaction; Plant-based diet.
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00146/25711/26299.pdf

Total replacement of fish oil by soybean or linseed oil with a return to fish oil in turbot (Psetta maxima) - 1. Growth performance, flesh fatty acid profile, and lipid metabolism
Provedor de dados: 5
Autores: Regost, Christelle; Arzel, Jacqueline; Robin, Jean; Rosenlund, Grethe; Kaushik, Sadasivam.
The aim of the study was to investigate the replacement of fish oil by vegetable oils and the effects of a washout with a return to fish oil on growth performances and lipid metabolism. three experimental fish meal-based isonitrogenous (crude protein content: 57.5%) and isolipidic (crude lipid content: 16.5%) diets were formulated containing either 9% of added fish oil (170), soybean oil (SO), or linseed oil (LO). Each diet was distributed to triplicate groups of 25 marketable size turbot (initial body weight of 579 g) grown in seawater at water temperature of 17 T. Fish were fed once a day to visual satiety. At the end of the growth trial, which lasted 13 weeks, all groups of turbot were fed with FO diet for 8 weeks. The growth of turbot was high, but the...
Tipo: Text
Palavras-chave: Lipogenesis; Fatty acids; Liver; Muscle; Body composition; Growth; Vegetable oil; Fish oil; Psetta maxima.
Ano: 2003
URL: http://archimer.ifremer.fr/doc/2003/publication-375.pdf

Regulation of the somatotropic axis by dietary factors in rainbow trout (Oncorhynchus mykiss)
Provedor de dados: 5
Autores: Gomez Requeni, P; Calduch Giner, J; De Celis, S; Medale, Francoise; Kaushik, Sadasivam; Perez Sanchez, J.
The activity of the somatotropic axis was analysed in juvenile rainbow trout (Oncorhynchus mykiss) fed either a fishmeal-based diet (FM) or graded levels of plant proteins to replace 50 % (PP50 diet), 75 % (PP75 diet) or 100 % (PP100 diet) of the fishmeal protein. For this purpose, partial cloning and sequencing of the gene encoding rainbow trout growth hormone receptor (GHR) was first accomplished by RT-PCR, using degenerate primers based on the sequences of non-salmonid fish GHR. Growth rates and energy retention were lowered by the PP75 and PP100 diets and a concurrent and progressive increase in plasma levels of growth hormone (GH) was found. However, no changes in hepatic GH binding and total plasma insulin-like growth factor (IGF)-I levels were...
Tipo: Text
Palavras-chave: Insulin like growth factor; Growth hormone receptor; Growth hormone; Plant proteins; Rainbow trout.
Ano: 2005
URL: http://archimer.ifremer.fr/doc/2005/publication-3620.pdf

The time course of fish oil wash-out follows a simple dilution model in gilthead sea bream (Sparus aurata L.) fed graded levels of vegetable oils
Provedor de dados: 5
Autores: Benedito-palos, Laura; Navarro, Juan C.; Bermejo-nogales, Azucena; Saera-vila, Alfonso; Kaushik, Sadasivam; Perez-sanchez, Jaume.
The aim of the study was to determine whether changes in the tissue fatty acid (FA) profile follows a simple test dilution model after changing the dietary oil sources in gilthead sea bream. A 14-month trial was conducted with juvenile fish of 18 g initial body weight fed either a fish oil-based diet (FO diet) or vegetable oils replacing 33% (33VO) and 66% (66VO) of fish oil. The trial included 3 months feeding a fish oil finishing diet to follow the restoration of the FA profile with the FO diet. Fish oil replacement with/without a finishing phase of fish oil re-feeding did not affect growth and all groups reached 520-531 g body weight. Changes in body composition with weight gain did not modify the FA profile of fish continuously fed FO, 33VO or 66VO...
Tipo: Text
Palavras-chave: Plant proteins; Fatty acids; Flesh; Growth; Fish.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/2009/publication-6318.pdf

Whole body amino acid composition of European seabass (Dicentrarchus labrax), gilthead seabream (Sparus aurata) and turbot (Psetta maxima) with an estimation of their IAA requirement profiles
Provedor de dados: 5
Autores: Kaushik, Sadasivam.
The amino acid compositions of whole body tissue of European seabass, gilthead seabream and turbot of two different size classes were determined. No significant differences were detected between species and the composition was not affected by body size. Based on whole body indispensable amino acids (IAA) to total IAA ratios (A/E ratios), the IAA requirement profiles for the three species were estimated and compared to data available to date for some amino acids, obtained using dose-response curves.
Tipo: Text
Palavras-chave: Indispensable amino acids; Whole body composition; Requirement profile; Nutrition; Marine; Teleosts.
Ano: 1998
URL: https://archimer.ifremer.fr/doc/1998/publication-868.pdf

Hepatic phosphoenolpyruvate carboxykinase gene expression is not repressed by dietary carbohydrates in rainbow trout (Oncorhynchus mykiss)
Provedor de dados: 5
Autores: Panserat, Stéphane; Plagnes Juan, E; Breque, Jeanine; Kaushik, Sadasivam.
Phosphoenolpyruvate carboxykinase (PEPCK) is a rate-limiting enzyme in hepatic gluconeogenesis and therefore plays a central role in glucose homeostasis. The aim of this study was to analyse the nutritional regulation of PEPCK gene expression in rainbow trout (Oncorhynchus mykiss), which are known to use dietary carbohydrates poorly. A full-length hepatic PEPCK cDNA (2637 base pairs with one open reading frame putatively encoding a 635-residue protein) was cloned and found to be highly homologous to mammalian PEPCKs. The presence of a putative peptide signal specific to a mitochondrial-type PEPCK in the deduced amino acid sequence suggests that this PEPCK gene codes for a mitochondrial form, In gluconeogenic tissues such as liver, kidney and intestine,...
Tipo: Text
Palavras-chave: Gluconeogenesis; Nutrition; Metabolism; Glucose; Oncorhynchus mykiss; Rainbow trout; Phosphoenolpyruvate carboxykinase.
Ano: 2001
URL: http://archimer.ifremer.fr/doc/2001/publication-414.pdf

Lack of significant long-term effect of dietary carbohydrates on hepatic glucose-6-phosphatase expression in rainbow trout (Oncorhynchus mykiss)
Provedor de dados: 5
Autores: Panserat, Stéphane; Medale, Francoise; Breque, Jeanine; Plagnes Juan, E; Kaushik, Sadasivam.
Hepatic glucose-6-phosphatase (G6Pase) plays an important role in glucose metabolism because it catalyzes the release of glucose to the circulatory system in the processes of glycogenolysis and gluconeogenesis. The present study was initiated to analyze the regulation of hepatic G6Pase expression by dietary carbohydrates in rainbow trout. The first step in our study was the identification of a partial G6Pase cDNA in rainbow trout that was highly homologous to that of mammals. Hepatic G6Pase activities and mRNA levels were measured in trout fed one of the experimental diets, with or without carbohydrates. We found no significant effect of intake of dietary carbohydrates on G6Pase Expression (mRNA and activity) 6 hours and 24 hours after feeding. These...
Tipo: Text
Palavras-chave: Dietary carbohydrates; Rainbow trout; Fish nutrition; Glucose 6 phosphatase expression.
Ano: 2000
URL: http://archimer.ifremer.fr/doc/2000/publication-696.pdf

Effect of long-term feeding with a plant protein mixture based diet on growth and body/fillet quality traits of large rainbow trout (Oncorhynchus mykiss)
Provedor de dados: 5
Autores: De Francesco, Matilde; Parisi, Giuliana; Medale, Francoise; Lupi, Paola; Kaushik, Sadasivam; Poli, Bianca.
Two diets based oil fish meal (FM) or oil a mixture of plant protein sources supplemented with free amino acids (PP) were fed to rainbow trout having a mean initial body weight of 162.5 g reared at 17 +/- 1 degreesC for 24 weeks to evaluate the effect of plant protein sources oil fish growth, morphological and body quality traits. At the end of the growth study, samples were withdrawn for chemical analyses as well as measurements of different parameters of quality.   Compared to the FM group, the trout fed diet PP had lower growth rates (mean final body weight 663 vs. 800 g), feed efficiency (0.95 vs. 1.07) and protein efficiency ratio (1.96 vs. 2.13). Trout fed diet PP were also characterised by a lower dressed weight (89.45% vs. 91.07%), hepato-somatic...
Tipo: Text
Palavras-chave: Sensory evaluation; Chemical composition quality; Flesh; Body traits; Plant protein; Oncorhynchus mykiss.
Ano: 2004
URL: http://archimer.ifremer.fr/doc/2004/publication-391.pdf

High levels of vegetable oils in plant protein-rich diets fed to gilthead sea bream (Sparus aurata L.): growth performance, muscle fatty acid profiles and histological alterations of target tissues
Provedor de dados: 5
Autores: Benedito Palos, L; Navarro, J; Sitja Bobadilla, A; Bell, J; Kaushik, Sadasivam; Perez Sanchez, J.
The feasibility of fish oil (FO) replacement by vegetable oils (VO) was investigated in gilthead sea bream (Sparus aurata L.) in a growth trial conducted for the duration of 8 months. Four isolipidic and isoproteic diets rich in plant proteins were Supplemented with L-lysine (0-55%) and soya lecithin (1%). Added oil was either FO (control) or a blend of VO, replacing 33% (33VO diet), 66% (66VO diet) and 100% (VO diet) of FO. No detrimental effects on growth performance were found with the partial FO replacement, but feed intake and growth rates were reduced by about 10% in fish fed the VO diet. The replacement strategy did not damage the intestinal epithelium, and massive accumulation of lipid droplets was not found within enterocytes. All fish showed...
Tipo: Text
Palavras-chave: Lipoid liver disease; Soya lecithin; Phospholipids; Essential fatty acids.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/publication-4780.pdf

Influence of the diet on the microbial diversity of faecal and gastrointestinal contents in gilthead sea bream (Sparus aurata) and intestinal contents in goldfish (Carassius auratus)
Provedor de dados: 5
Autores: De Paula Silva, Flavia Cristina; Nicoli, Jacques Robert; Zambonino, Jose Luiz; Kaushik, Sadasivam; Gatesoupe, François-joel.
Fish intestinal microbiota changes with diet and this effect is of particular interest considering the increasing substitution of fish meal by plant protein sources. The objective of this work was to study the effects of partial substitution of fish meal with lupin and rapeseed meals on gut microbiota of the gilthead sea bream (Sparus aurata) and in goldfish (Carassius auratus). Faecal, gastrointestinal and intestinal contents were characterized by culture-based and molecular methods. Vibrionaceae was high in faeces and in the intestine of sea bream, while a more diverse microbiota was retrieved from the stomach, where Bacillales and Flavobacteriaceae appeared to be influenced by the diet. PCR-DGGE profiles revealed high diversity of the microbiota...
Tipo: Text
Palavras-chave: Vegetable meals; DGGE; Microbiota; Stomach; Intestine; Faeces.
Ano: 2011
URL: http://archimer.ifremer.fr/doc/00040/15096/12440.pdf

Gluconeogenic enzyme gene expression is decreased by dietary carbohydrates in common carp (Cyprinus carpio) and gilthead seabream (Sparus aurata)
Provedor de dados: 5
Autores: Panserat, Stéphane; Plagnes Juan, E; Kaushik, Sadasivam.
Our objective is to understand the low metabolic utilization of dietary carbohydrates in fish. We compared the regulation of gluconeogenic enzymes at a molecular level in two fish species, the common carp (Cyprinus carpio) and gilthead seabream (Sparus aurata), known to be relatively tolerant to dietary carbohydrates. After cloning of partial cDNA sequences for three key gluconeogenic enzymes (glucose-6-phosphatase (G6Pase), fructose biphosphatase (FBPase) and phosphoenolpyruvate carboxykinase (PEPCK) in the two species, we analyzed gene expressions of these enzymes 6 and 24 h after feeding with (20%) or without carbohydrates. Our data show that there is at least one gluconeogenic enzyme strongly regulated (decreased expression after feeding) in the two...
Tipo: Text
Palavras-chave: Gilthead seabream; Common carp; Gluconeogenesis; Gene expression; Hepatic glucose metabolism; Dietary carbohydrate.
Ano: 2002
URL: http://archimer.ifremer.fr/doc/2002/publication-668.pdf

Molecular cloning, tissue distribution and sequence analysis of complete glucokinase cDNAs from gilthead seabream (Sparus aurata), rainbow trout (Oncorhynchus mykiss) and common carp (Cyprinus carpio)
Provedor de dados: 5
Autores: Panserat, Stéphane; Blin, C; Medale, Francoise; Plagnes Juan, E; Breque, Jeanine; Krishnamoorthy, J; Kaushik, Sadasivam.
The enzyme glucokinase (GK) (EC 2.7.1.1) plays an important role in the control of glucose homeostasis. Qualitative and/or quantitative variations in GK enzyme have been postulated by previous studies to explain why dietary carbohydrate utilisation is lower in gilthead seabream (Sparus aurata) and rainbow trout (Oncorhynchus mykiss) than in common carp (Cyprinus carpio). In this study, we report the isolation and characterisation of a full-length cDNA coding for GK in these teleosts. Amino acid sequences derived from these cDNA clones are highly similar to other vertebrate GKs. These findings, including a detailed phylogenetic analysis, reveal that GK gene highly homologous to mammalian GK exists in these fish species with similar tissue specific...
Tipo: Text
Palavras-chave: Glucose phosphorylation; Dietary carbohydrate; Fish nutrition.
Ano: 2000
URL: http://archimer.ifremer.fr/doc/2000/publication-669.pdf

Reduced lipid intake leads to changes in digestive enzymes in the intestine but has minor effect on key enzymes of hepatic intermediary metabolism in rainbow trout (Oncorhynchus mykiss)
Provedor de dados: 5
Autores: Ducasse Cabanot, S; Zambonino, Jose-luis; Richard, N; Medale, Francoise; Corraze, Genevieve; Mambrini, M; Robin, Jean; Cahu, Chantal; Kaushik, Sadasivam; Panserat, S.
For sustainable aquaculture, the removal of marine resource ingredients in fish diets is an important objective. While most studies focus on the replacement of fish oil by vegetable oil, little is known on the nutritional effects of presence (which corresponds to the control diet) or absence of dietary fish oil. We studied fatty acid composition of brush-border membranes and digestive enzyme activities of the intestine and measured the expression and activities of several enzymes involved in the hepatic intermediary metabolism of rainbow trout (Oncorhynchus mykiss) fed for 7 weeks with or without fish oil. The diets were pair-fed to ensure that fish fed either diet had comparable carbohydrate and protein intakes. Absence of fish oil significantly reduced...
Tipo: Text
Palavras-chave: Rainbow trout; Metabolism; Fish oil; Digestion; Aquaculture.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/publication-3506.pdf

Effects of rapeseed meal-glucosinolates on thyroid metabolism and feed utilization in rainbow trout
Provedor de dados: 5
Autores: Burel, Christine; Boujard, Thierry; Kaushik, Sadasivam; Boeuf, Gilles; Mol, K; Van Der Geyten, S; Darras, V; Kuhn, E; Pradet Balade, B; Querat, B; Quinsac, A; Krouti, M; Ribaillier, D.
Two rapeseed meals (RM1 and RM2), containing glucosinolates at a concentration of 26 and 40 mumol/g, respectively, were incorporated at increasing levels (10, 20, and 30% for RM1 and 30 and 50% for RM2) in diets of juvenile rainbow trout. Disturbances in the thyroid axis appeared after 14 days of feeding (with a dietary incorporation level of 10%). The dietary supplementation with T-3 or iodine induced an increase in plasma T-3 levels, compared to that in fish fed the RM diets, and reduced the deleterious effect of RM on growth. When trout were reared in seawater, there was also a slight increase in thyroid hormone levels. TSH treatment had no effect on the thyroid hormone plasma levels. The incorporation of 30% of RM1, which induced a lower dietary...
Tipo: Text
Palavras-chave: Rainbow trout; Iodine; TSH; Deiodinase activity; Thyroid function; Growth; Glucosinolates; Rapeseed.
Ano: 2001
URL: http://archimer.ifremer.fr/doc/2001/publication-677.pdf

The effect of nucleic acids on growth, ureagenesis and nitrogen excretion of gilthead sea bream Sparus aurata juveniles
Provedor de dados: 5
Autores: Oliva Teles, A; Guedes, M; Vachot, Christianne; Kaushik, Sadasivam.
The aim of this trial was to evaluate the effect of dietary nucleic acids on ureagenesis and nitrogen balance in gilthead sea bream juveniles. For that purpose, 5 isonitrogenous (7.25% N) diets were formulated based on fish meal as protein source (FM-control diet) and to partially replace the fish meal nitrogen with two levels of nucleic acids (diets RNA1 and RNA2) or brewers yeast (diets BY1 and BY2). Each diet was fed for 10 weeks, to apparent visual satiety, to triplicate groups of 50 fish each with an average body weight of 12.7 g.   Inclusion of nucleic acid N either as brewers yeast or RNA led to significant improvement of feed intake and growth, except for fish fed diet RNA2. There were no differences in final weight between groups fed the yeast-...
Tipo: Text
Palavras-chave: Sea bream; N accretion; Ureagenesis; Brewers yeast; RNA.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/publication-6681.pdf

Rapid metabolic adaptation in European sea bass (Dicentrarchus labrax) juveniles fed different carbohydrate sources after heat shock stress
Provedor de dados: 5
Autores: Enes, P; Panserat, S; Kaushik, Sadasivam; Oliva Teles, A.
A study was conducted to evaluate the effect of two dietary carbohydrate sources (waxy maize starch and glucose) on the metabolic adaptation of sea bass juveniles (initial weight: 24 g) to a heat shock treatment (temperature rise from 18 degrees C to 25 degrees C within 24 h). Two isonitrogenous and isolipidic diets were fon-nulated to contain 20% waxy maize starch (WS diet) or 20% glucose (GLU diet). Triplicate groups of fish were fed to near satiation for 4 weeks at both temperatures (18 degrees C and 25 degrees C). Then, fish previously maintained at 18 degrees C were submitted to a heat shock (18 degrees C to 25 degrees C) and continued to be fed with the same diets during I more week. The higher water temperature significantly improved growth...
Tipo: Text
Palavras-chave: Waxy maize starch; Temperature; Lipogenic enzymes; HSP70; Glycolytic enzymes; Glucose; Gluconeogenic enzymes; European sea bass; Carbohydrate utilization; Biomarker.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/publication-6958.pdf

Natural abundance of N-15 and C-13 in fish tissues and the use of stable isotopes as dietary protein tracers in rainbow trout and gilthead sea bream
Provedor de dados: 5
Autores: Beltran, M.; Fernandez-borras, J.; Medale, Francoise; Perez-sanchez, J.; Kaushik, Sadasivam; Blasco, J.
For developing efficient diets, two sets of experiments examined whether the use and allocation of dietary protein can be traced by labelling with stable isotopes (N-15 and C-13) in two culture fish (Oncorhynchus mykiss and Sparus aurata). In the first experiment, natural abundance and tissue distribution of these isotopes were determined, by measuring the delta C-13 and delta N-15 values by isotopic ratio mass spectrometry, in fingerlings (14-17 g) adapted to diets differing in the percentage of fish meal replacement by plant protein sources. For both species, delta N-15 and delta C-13 were greater in tissues with higher protein and lower lipid content. Delta N-15 of diets and tissues decreased as replacement increased, suggesting delta N-15 can be used...
Tipo: Text
Palavras-chave: Sea bream; Rainbow trout; Protein; Diet; Delta N 15; Delta C 13.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/2009/publication-6966.pdf

A first insight into genotype x diet interactions in European sea bass (Dicentrarchus labrax L. 1756) in the context of plant-based diet use
Provedor de dados: 5
Autores: Le Boucher, Richard; Vandeputte, Marc; Dupont-nivet, Mathilde; Quillet, Edwige; Mazurais, David; Robin, Jean; Vergnet, Alain; Medale, Francoise; Kaushik, Sadasivam; Chatain, Beatrice.
This preliminary study assessed genotype x diet interaction in late growth of European sea bass (Dicentrarchus labrax) fed with either a fish meal (FM)- or a fish oil (FO)-based diet (M) or an all-plant-based (PB) diet. A total of 550 fish from 224 families were reared together and tagged. DNA was sampled and microsatellites were used to assign parentage. When fish weight was 192 +/- 54 g, two tanks were fed with M (FM: 100%; FO: 100%) and two others with PB (FM: 0% and FO: 0%). Body weight (BW), fork length (FL) and fillet lipid content (CorrFat) were analysed with a linear model and with REML methodology. We observed no significant differences between groups, but a slightly lower (P=0.03) daily growth coefficient in sea bass fed PB than in those fed M....
Tipo: Text
Palavras-chave: Genotype by diet x environment interaction; European sea bass; Plant diet; Genetic correlation.
Ano: 2011
URL: http://archimer.ifremer.fr/doc/00032/14335/12374.pdf

Combined replacement of fish meal and oil in practical diets for fast growing juveniles of gilthead sea bream (Sparus aurata L.): Networking of systemic and local components of GH/IGF axis
Provedor de dados: 5
Autores: Benedito Palos, L; Saera Vila, A; Calduch Giner, J; Kaushik, Sadasivam; Perez Sanchez, J.
Growth performance and growth regulatory pathways were examined in juvenile gilthead sea bream fed diets containing largely plant-based ingredients. Four isonitrogenous and isolipidic extruded diets with a low level (20%) of fish meal inclusion were formulated with graded levels of a vegetable oil mixture (17:58:25 of rapeseed: linseed: palm oils) replacing fish oil at 33, 66 and 100% (33VO, 66VO and VO diets). All diets were supplemented with lysine (0.55%) and contained soy lecithin (1%). Daily growth coefficients and feed efficiency over the course of an 11-week trial were almost identical in fish fed the FO, 33VO and 66VO diets. The VO diet reduced feed intake and growth without significant effects in proximate whole body composition, nitrogen or...
Tipo: Text
Palavras-chave: Contaminants; Endocrine disrupters; Insulin like growth factors; Growth hormone receptors; Growth hormone; Plant proteins; Vegetable oil; Fish oil; Sparidae.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/publication-5017.pdf

FADS2: A potential limiting step in HUFA metabolic pathway of European sea bass (Dicentrarchus labrax)
Provedor de dados: 5
Autores: Geay, E; Santigosa I Culi, E; Corporeau, Charlotte; Boudry, Pierre; Dreano, Y; Corcos, L; Bodin, Nathalie; Kaushik, Sadasivam; Vandeputte, Marc; Zambonino, Jose-luis; Mazurais, David; Cahu, Chantal.
Tipo: Text
Ano: 2010

Influence of lupin and rapeseed meals on the integrity of digestive tract and organs in gilthead seabream (Sparus aurata L.) and goldfish (Carassius auratus L.) juveniles
Provedor de dados: 5
Autores: Omnes, Marie-helene; Silva, Francisco; Moriceau, Jacques; Aguirre, P.; Kaushik, Sadasivam; Gatesoupe, F. J.
Two rearing trials were conducted to evaluate the effects of dietary lupin (LM) and rapeseed (RM) meals in gilthead seabream (Sparus aurata L.) and goldfish (Carassius auratus L.) juveniles. Each plant meal was incorporated at the rate of 200 g kg−1 in two distinct diets, which were compared with a fishmeal-based diet as control. After 1 month, the two plant diets did not influence the whole body growth, but the digestive systems were affected. The splenosomatic index was reduced with two plant meals in goldfish and with RM in seabream. The hepatosomatic index was only reduced in LM-fed seabream. Cellular characteristics were also affected. The largest liver cells were observed in RM-fed goldfish suggesting changes in metabolic function. The LM and RM...
Tipo: Text
Palavras-chave: Digestive tract; Gilthead seabream; Goldfish; Histology; Lupin; Rapeseed.
Ano: 2015
URL: https://archimer.ifremer.fr/doc/00190/30107/28742.pdf

Differential gene expression after total replacement of dietary fish meal and fish oil by plant products in rainbow trout (Oncorhynchus mykiss) liver
Provedor de dados: 5
Autores: Panserat, S.; Hortopan, G. A.; Plagnes-juan, E.; Kolditz, C.; Lansard, M.; Skiba-cassy, S.; Esquerre, D.; Geurden, I.; Medale, Francoise; Kaushik, Sadasivam; Corraze, Genevieve.
Our objective was to analyse the hepatic transcriptomes of juvenile rainbow trout fed with a plant-based diet. We focused our analysis on the total replacement of fish meal (FM) and fish oil (FO) by a 100% plant-based diet (0% FM, 0% FO). We analysed the postprandial hepatic transcriptomes of rainbow trout fed the two diets 8 h after feeding. Six total hepatic RNAs from each dietary group were hybridised against a trout cDNA microarray (9K). After treatment of the data respecting the standard MIAME (Minimum Information About a Microarray Experiment) protocol, we found that 176 hepatic genes were differentially expressed between fish fed the two diets: 96 and 80 were over-expressed and under-expressed, respectively, in trout fed the plant-based diet. A...
Tipo: Text
Palavras-chave: Rainbow trout; Transcriptome; Metabolism; Liver; Vegetable products; Marine ingredients.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/2009/publication-6684.pdf

Total replacement of fish oil by soybean or linseed oil with a return to fish oil in Turbot (Psetta maxima) 2. Flesh quality properties
Provedor de dados: 5
Autores: Regost, Christelle; Arzel, Jacqueline; Cardinal, Mireille; Rosenlund, Grethe; Kaushik, Sadasivam.
The aim of this study was to evaluate (1) the effects of replacement of fish oil by vegetable oils on flesh quality and (2) the effects of a washout with a return to fish oil on flesh quality of turbot. In a first period of 3 months, three isonitrogenous and isolipidic diets containing 9% of added marine fish oil (170), soybean oil (SO) or linseed oil (LO) were fed to triplicate groups of 25 marketable size turbot (initial body weight: 579 +/- 1 g) grown in sea water at the temperature of 17 degreesC. At the end of the first period, all groups of turbot were fed with the diet containing fish oil (diet FO) for a further period of 2 months. The gutted and fillet yields were not affected by the incorporation of vegetable oils. However, soybean or linseed oils...
Tipo: Text
Palavras-chave: Sensory analyses; Texture; Washout period; Vegetable oils; Psetta maxima.
Ano: 2003
URL: http://archimer.ifremer.fr/doc/2003/publication-385.pdf

Effect of fish meal replacement by plant protein sources on non-specific defence mechanisms and oxidative stress in gilthead sea bream (Sparus aurata)
Provedor de dados: 5
Autores: Sitja Bobadilla, A; Pena Llopis, S; Gomez Requeni, P; Medale, Francoise; Kaushik, Sadasivam; Perez Sanchez, J.
Partial or total replacement of fish meal by a mixture of plant protein (PP) sources (corn gluten, wheat gluten, extruded peas, rapeseed meal and sweet white lupin) balanced with indispensable amino acids was examined in juvenile gilthead sea bream over the course of a 6-month growth trial. A diet with fish meal (FM) as the sole protein source was compared to diets with 50%, 75% and 100% of replacement (PP50, PP75, and PP100). The possible influence of diets on growth performance, plasma metabolites, gut integrity, liver structure, anti-oxidant and immune status was evaluated. Final body weight was progressively decreased with PP inclusion, but in PP50 and PP75-fed fish, feed efficiency (FE) was significantly improved and specific growth rates remained...
Tipo: Text
Palavras-chave: Gilthead sea bream; Cholesterol; Glutathione; Histopathology; Myeloperoxidase; Complement; Lysozyme; Respiratory burst; Immune response; Growth; Plant proteins; Fish meal.
Ano: 2005
URL: http://archimer.ifremer.fr/doc/2005/publication-3595.pdf

Regulation of feed intake, growth, nutrient and energy utilisation in European sea bass (Dicentrarchus labrax) fed high fat diets
Provedor de dados: 5
Autores: Boujard, Thierry; Gelineau, Anne; Coves, Denis; Corraze, Genevieve; Dutto, Gilbert; Gasset, Eric; Kaushik, Sadasivam.
Three practical isoproteic (54% protein) diets were formulated to contain graded levels of crude fat (diet L: 10%, diet M: 20% and diet H: 30%). Each diet was assigned unrestrictedly to three and restrictedly to two replicate groups of fish (IBW 243 g). In unrestricted groups, increasing the dietary lipid level led to a significant decrease in voluntary feed intake without affecting growth rate. In the feed-restricted groups, daily growth rates increased with increasing dietary fat levels. There was a significant and inverse effect of the dietary fat content on whole body moisture and fat levels, with highest lipid (ca. 20%) and lowest moisture (ca. 58%) contents in sea bass fed diet containing the highest lipid level; muscle lipid concentration was...
Tipo: Text
Palavras-chave: Phosphorus excretion; Feed intake; Lipid; Digestible energy; European sea bass; Dicentrarchus labrax.
Ano: 2004
URL: http://archimer.ifremer.fr/doc/2004/publication-392.pdf

The effects of dietary carbohydrate sources and forms on metabolic response and intestinal microbiota in sea bass juveniles, Dicentrarchus labrax
Provedor de dados: 5
Autores: Gatesoupe, Joel; Huelvan, Christine; Le Bayon, Nicolas; Severe, Armelle; Aasen, Inga Marie; Degnes, Kristin F.; Mazurais, David; Panserat, Stephane; Zambonino, Jose-luis; Kaushik, Sadasivam.
The aim of this work was to investigate the catabolic process of three kinds of dietary carbohydrates in the gut of sea bass juveniles, with the possible contribution of the intestinal microbiota to the nutrition of the host, and the subsequent effects on intermediary metabolism. A first diet contained waxy maize (99% amylopectin), a highly digestible form of starch. A second diet was less quickly digestible due to its high amylose content of resistant starch. Two other diets contained fibre instead of starch, either only cellulose as control, or also other non-starch polysaccharides brought by lupin meal. The effect of the diets on the host confirmed previous results, with the stimulation of glucose storage in the liver in sea bass fed the starchy diets,...
Tipo: Text
Palavras-chave: Digestive enzymes; Glucose metabolism; Intestinal microbiota; Viscerosomatic index; Gene expression; Metabolite analyses; Vegetable feedstuffs.
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00166/27763/25944.pdf

Dietary lipid level, hepatic lipogenesis and flesh quality in turbot (Psetta maxima)
Provedor de dados: 5
Autores: Regost, Christelle; Arzel, Jacqueline; Cardinal, Mireille; Robin, Jean; Laroche, M; Kaushik, Sadasivam.
A study was undertaken with marketable size turbot to evaluate the effects of dietary fat levels on chemical composition. lipogenesis and flesh quality, Four experimental diets containing graded levels of fish oil in order to obtain 10%, 15%, 20% and 25% of crude fat were fed to triplicate groups of turbot (initial body weight of 660 g) for 12 weeks in full strength seawater at temperature of 17 degreesC. Nutrient digestibility was not influenced by dietary fat levels. The best growth performance was observed in fish fed 10% and 15% dietary fat. High dietary lipid levels led to higher fat deposition in whole fish, although lipid level in muscle remained low (1.1% in dorsal muscle and 1.7% in ventral muscle irrespective of diet), Significant subcutaneous...
Tipo: Text
Palavras-chave: Flesh quality; Lipogenesis; Fat deposition; Dietary lipids; Psetta maxima.
Ano: 2001
URL: http://archimer.ifremer.fr/doc/2001/publication-376.pdf

Effect of genistein-enriched diets on the endocrine process of gametogenesis and on reproduction efficiency of the rainbow trout Oncorhynchus mykiss
Provedor de dados: 5
Autores: Bennetau Pelissero, Catherine; Breton, Bernard; Bennetau, Bernard; Corraze, Genevieve; Le Menn, Françoise; Davail Cuisset, Blandine; Helou, Chantal; Kaushik, Sadasivam.
Three practical diets were formulated to contain 0, 500, or 1000 ppm genistein. The three diets were distributed for 1 year to groups of rainbow trout undergoing their first gametogenesis and until spawning. Growth performance of rainbow trout was not affected by dietary treatments. Plasma cholesterol levels were equivalent between groups. In males, a slight but constant induction of vitellogenin (VTG) synthesis and a decrease in testosterone levels were observed. A slight decrease in plasma levels of beta FSH and beta LH was noticed at the end of spermatogenesis in the male fish fed a diet with 500 ppm (genistein) (from 2.16 +/- 0.39 to 1.47 +/- 0.23 for beta FSH and from 0.44 +/- 0.09 to 0.31 +/- 0.09 for beta LH). There was a significantly reduced 17...
Tipo: Text
Palavras-chave: Diet; Reproduction; GTH; Vitellogenin; Steroid; Trout; Genistein.
Ano: 2001
URL: http://archimer.ifremer.fr/doc/2001/publication-738.pdf

Influence of partial substitution of dietary fish meal on the activity of digestive enzymes in the intestinal brush border membrane of gilthead sea bream, Sparus aurata and goldfish, Carassius auratus
Provedor de dados: 5
Autores: Silva, Flavia; Nicoli, Jacques R.; Zambonino, Jose-luis; Le Gall, Marie-madeleine; Kaushik, Sadasivam; Gatesoupe, François-joel.
The sustainable composition of diets of high nutritional value is of the utmost importance for intensive aquaculture. Digestion and absorption of nutrients depends on the activity of the digestive enzymes, in particular those located in the brush border membrane of enterocytes, which are responsible for the final stages of breaking down and absorption of nutrients. In the present study, the substitution of fish meal by lupin or rapeseed meal in the diet was evaluated on gilthead sea bream (Sparus aurata) and goldfish (Carassius auratus). The objectives were to compare the activities of intestinal brush border enzymes in both species fed the control and experimental diets. When gilthead sea bream were fed the vegetable diets, significantly lower activities...
Tipo: Text
Palavras-chave: Sparus aurata; Carassius auratus; Fish meal replacement; Brush border membrane; Digestive enzymes; Alkaline phosphatase; Aminopeptidase N; Maltase; Glutamyl transpeptidase.
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00009/11975/8679.pdf

Fatty acid profile of fish following a change in dietary fatty acid source: model of fatty acid composition with a dilution hypothesis
Provedor de dados: 5
Autores: Robin, Jean; Regost, Christelle; Arzel, Jacqueline; Kaushik, Sadasivam.
The fatty acid (FA) content of fish is generally said to reflect fatty acid composition of the diet. In fact, incorporation of FA into tissues is modulated by various metabolic factors, and final composition will depend upon the initial FA content, cumulative intake of dietary fatty acids, growth rate and duration. Analysis of time course of changes in FA composition should be easier with animals having different initial FA profiles, which are subsequently fed a diet with the same FA composition.   Data from two studies, one with brown trout and another with turbot were used. Fish were first fed with diets containing one of three different oils (soybean oil (SO), linseed oil (LO) and fish oil (FO)), and subsequently fed the same fish oil-based diet...
Tipo: Text
Palavras-chave: Dietary change; Model; Fish; Fatty acids.
Ano: 2003
URL: http://archimer.ifremer.fr/doc/2003/publication-498.pdf

A first insight into genotype-diet interactions in European seabass (Dicentrarchus labrax) in the context of plant-based diet use
Provedor de dados: 5
Autores: Le Boucher, Richard; Vandeputte, Marc; Dupont Nivet, Mathilde; Quillet, Edwige; Mazurais, David; Robin, Jean; Vergnet, Alain; Medale, Francoise; Kaushik, Sadasivam; Chatain, Beatrice.
Tipo: Text
Ano: 2009
URL: http://archimer.ifremer.fr/doc/00038/14907/12233.pdf

Nutritional bioenergetics and estimation of waste production in non-salmonids
Provedor de dados: 5
Autores: Kaushik, Sadasivam.
The general principles behind the bioenergetic approach for predicting growth, as well as for decreasing feed and nutrient losses, have been set forth for salmonids. Given the diversification of fish farming activities around the world and the ever increasing concern for water quality management, it becomes essential to verify whether an approach developed for salmonids is applicable to other species. Given this general background, an attempt is made here to check the theoretical assumptions and technical considerations behind the bioenergetic principles developed for rainbow trout with other freshwater or marine species. From a conceptual point of view, recent literature data do indicate that as far as nitrogen or energy balance is concerned, the general...
Tipo: Text
Palavras-chave: Nutrition; Croissance; Poissons d'eau douce; Poissons de mer; Nutrition; Growth; Freshwater fish; Marine fish.
Ano: 1998
URL: https://archimer.ifremer.fr/doc/1998/publication-869.pdf

Voluntary feed intake, nitrogen and phosphorus losses in rainbow trout (Oncorhynchus mykiss) fed increasing dietary levels of soy protein concentrate
Provedor de dados: 5
Autores: Medale, Francoise; Boujard, Thierry; Vallee, Frédéric; Blanc, Denise; Mambrini, Muriel; Roem, Arjen; Kaushik, Sadasivam.
High energy extruded diets were formulated to contain the same level of protein supplied either by soy protein concentrate (SPC) or fish meal. Three experiments were performed in order to measure voluntary feed intake and feed waste, faecal losses and soluble losses of nitrogen and phosphorus in rainbow trout (average body weight: 100 g). Voluntary feed intake and growth performance of fish fed with demand feeders were not different when diets contained 0, 50 or 75 % SPC instead of fish meal. Total replacement of fish meal by SPC led to a significant decrease in feed intake and resulted in poor growth. This was partly due to methionine deficiency in the SPC based diet. With the addition of crystalline DL-methionine in the diets, an improvement of feed...
Tipo: Text
Palavras-chave: Digestibilité; Excrétion; Azote; Phosphore; Protéine de soja; Ingestion volontaire; Truite arc-en-ciel; Digestibility; Excretion; Nitrogen; Phosphorus; Soy protein; Voluntary feed intake; Rainbow trout.
Ano: 1998
URL: https://archimer.ifremer.fr/doc/1998/publication-871.pdf

Nutritional regulation and tissue specificity of gene expression for proteins involved in hepatic glucose metabolism in rainbow trout (Oncorhynchus mykiss)
Provedor de dados: 5
Autores: Panserat, Stéphane; Plagnes Juan, E; Kaushik, Sadasivam.
Rainbow trout (Oncorhynchus mykiss) are known to use dietary carbohydrates poorly. One of the hypotheses to explain the poor utilisation of dietary glucose by these fish is a dysfunction in nutritional regulation of hepatic glucose metabolism. In this study, we obtained partial clones of rainbow trout cDNAs coding for a glucose transporter (Glut2), and for the enzymes 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (6PF-2K/F-2,6BPase), fructose-1,6-bisphosphatase (FBPase) and pyruvate kinase (PI(), Their deduced amino acid sequences were highly similar to those of mammals (up to 80% similarity), In a study of nutritional regulation, the Glut2 gene was highly expressed in the liver irrespective of the nutritional status of the trout, in agreement with...
Tipo: Text
Palavras-chave: Oncorhynchus mykiss; Trout; Bifunctional enzyme; Pyruvate kinase; Fructose bisphosphatase; Glucose transporter; Glucose metabolism; Liver metabolism.
Ano: 2001
URL: http://archimer.ifremer.fr/doc/2001/publication-415.pdf

Protein sparing effect of dietary lipids in common dentex (Dentex dentex): a comparative study with sea bream (Sparus aurata) and sea bass (Dicentrarchus labrax)
Provedor de dados: 5
Autores: Company, Rubén; Calduch Giner, Josep; Perez Sanchez, Jaume; Kaushik, Sadasivam.
The common dentex (Dentex dentex) is a sparid fish which is considered a suitable candidate for Mediterranean aquaculture. A seven-weeks feeding trial was conducted over the summer period with common dentex, sea bream (Sparus aurata) and sea bass (Dicentrarchus labrax). All three species were fed to visual satiety with two practical diets with varying crude protein and crude fat levels (55 % protein, 9 % lipids; 46 % protein, 17 % lipids). The initial body weights were 8.2, 11.6 and 17.3 g for common dentex, sea bass and sea bream, respectively. In all cases, specific growth rates were not significantly affected by dietary treatment, but they varied among species (sea bass 1.7-1.8 %; sea bream 2.1-2.3 %; common dentex 3.1-3.2 %). When comparisons between...
Tipo: Text
Palavras-chave: Sparus aurata; Dicentrarchus labrax; Dentex dentex; Growth hormone; Energy availability; Protein retention; Growth performance; Sparus aurata; Dentex dentex I Dicentrarchus labrax; Hormone de croissance; Energie digestible; Rétention protéinique; Croissance.
Ano: 1999
URL: http://archimer.ifremer.fr/doc/1999/publication-863.pdf

Effect of dietary phospholipid level on the development of gilthead sea bream (Sparus aurata) larvae fed a compound diet
Provedor de dados: 5
Autores: Seiliez, I; Bruant, J; Zambonino, Jose-luis; Kaushik, Sadasivam; Bergot, Pierre.
The aim of the study was to determine the influence of dietary phospholipid (PL) levels on survival and development of first feeding gilthead sea bream (Sparus aurata) larvae. Larvae were fed from day 4 to 23 posthatching with an isoproteic and isolipidic formulated diet with graded levels of PL from 90-150 g kg(-1) dry matter (DM). A dietary PL content of more than 90 g kg(-1) DM seems to be necessary for sustaining growth of first feeding sea bream larvae. The survival rates of larvae fed the formulated diets (31-40% at day 23) were similar to those generally observed in marine aquaculture hatcheries with live prey feeding sequence. However, this high survival rate was not associated with high growth and the larvae showed, at the end of the study, a high...
Tipo: Text
Palavras-chave: Sparus aurata; Phospholipids; Larvae; First feeding; Development; Compound diet.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/publication-1869.pdf

Contribution of dietary arginine to nitrogen utilisation and excretion in juvenile sea bass (Dicentrarchus labrax) fed diets differing in protein source
Provedor de dados: 5
Autores: Tulli, F; Vachot, Christianne; Tibaldi, E; Fournier, Vincent; Kaushik, Sadasivam.
The role of dietary arginine in affecting nitrogen utilisation and excretion was studied in juvenile European sea bass (Dicentrarchus labrax) fed for 72 days with diets differing in protein sources (plant protein-based (PM) and fish-meal-based (FM)). Fish growth performance and nitrogen utilisation revealed that dietary Arg surplus was beneficial only in PM diets. Dietary Arg level significantly affected postprandial plasma urea concentrations. Hepatic arginase activity increased (P &lt; 0.05) in response to dietary Arg surplus in fish fed plant protein diets; conversely ornithine transcarbamylase activity was very low and inversely related to arginine intake. No hepatic carbamoyl phosphate synthetase III activity was detected. Dietary arginine levels did...
Tipo: Text
Palavras-chave: Dicentrarchus labrax; Sea bass; Excretion; Arginase; Urea; Arginine.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/publication-6317.pdf

Effect of high-level fish meal replacement by plant proteins in gilthead sea bream (Sparus aurata) on growth and body/fillet quality traits
Provedor de dados: 5
Autores: De Francesco, M; Parisi, G; Perez Sanchez, J; Gomez Requeni, P; Medale, Francoise; Kaushik, Sadasivam; Mecatti, M; Poli, B.
Juvenile gilthead sea bream (initial body weight ca. 100 g) were reared in an indoor flow through marine water system for 1 year. Fish were fed two isoenergetic [19.2 kJ g&amp;#8722;1 dry matter (DM)] and isoproteic (426 g kg&amp;#8722;1 DM) diets either based on fish meal (diet FM) or on a mixture of plant protein sources (diet PP), replacing 75% of fish meal protein. The growth trial was conducted in duplicate, two tanks for each dietary treatment. Growth performance and feed utilization were registered. Fillet quality parameters were evaluated and sensory analyses on cooked fillet were performed. Both groups had similar weight gain and specific growth rates. Feed intake was higher in sea bream fed diet FM (0.48 versus 0.44), while feed efficiency and...
Tipo: Text
Palavras-chave: Sparus aurata; Sensory evaluation; Quality traits; Plant protein; Gilthead sea bream; Chemical composition.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/publication-6256.pdf