Consulta: creatorFacets:"Kawazu, Shin-ichiro"
Registros recuperados: 9
Data/hora: 11/06/2024 09:56:38

Identification and functional analysis of a novel mitochondria-localized 2-Cys peroxiredoxin, BbTPx-2, from Babesia bovis
Provedor de dados: 19
Autores: Masatani, Tatsunori; Asada, Masahito; Hakimi, Hassan; Hayashi, Kei; Yamagishi, Junya; Kawazu, Shin-ichiro; Xuan, Xuenan.
Cysteine-based peroxidases, known as peroxiredoxins (Prx) or thioredoxin peroxidases (TPx), are important antioxidant enzymes that prevent oxidative damage caused by reactive oxygen species (ROS). In this study, we identified a novel mitochondrial 2-Cys Prx, BbTPx-2, from a bovine Babesia parasite, B. bovis. BbTPx-2 complementary DNA (cDNA) encodes a polypeptide of 254 amino acid residues. This protein has a mitochondrial targeting peptide at the N-terminus and two conserved cysteine residues of the typical 2-Cys Prx. By using a thiol mixed-function oxidation assay, the antioxidant activity of recombinant BbTPx-2 was revealed, and its antioxidant activity was comparable to that of a cytosolic 2-Cys Prx from B. bovis, BbTPx-1. Notably, we confirmed that...
Palavras-chave: Antioxidant activity; Babasia bovis; Mitochondria; Peroxiredoxin.
Ano: 2016
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4487

Characterization of an epimastigote-stage-specific hemoglobin receptor of Trypanosoma congolense
Provedor de dados: 19
Autores: Yamasaki, Shino; Suganuma, Keisuke; Yamagishi, Junya; Asada, Masahito; Yokoyama, Naoaki; Kawazu, Shin-ichiro; Inoue, Noboru.
Background: Since Trypanosorna spp. lack a complete heme synthesis pathway, the parasites are totally dependent on their host for heme throughout all of the stages of their life -cycle. We herein report the identification and characterization of a T. congolense epimastigote form (EMF)-specific hemoglobin (Hb) receptor. The gene was initially reported to encode a T. congolense haptoglobin (Hp)-Hb complex receptor (TcHpHbR) based on its similarity to a gene encoding a T brucei Hp-Hb complex receptor (TbHpHbR). Methods: Trypanosorna congolense IL3000 was used in this study. A TcHpHbR gene was PCR amplified from the parasite genome. The recombinant protein was used as an immunogen to raise antibodies for immunofluorescence assay and immunoblotting. Hemoglobin...
Palavras-chave: Trypanosome congolense; Epimastigote; Hemoglobin receptor.
Ano: 2016
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4441

Identification of a novel B. gibsoni 27-kDa protein as a serodiagnostic antigen
Provedor de dados: 19
Autores: Terkawi, M. A.; Aboge, G.; Jia, H.; Goo, Y-K.; Ooka, H.; Yamagishi, Junya; Nishikawa, Yoshifumi; Kawazu, Shin-ichiro; Fujisaki, K.; Xuan, Xuenan; 山岸, 潤也; 西川, 義文; 河津, 信一郎; 玄, 学南.
A novel gene encoding 27-kDa protein was identified by the screening of Babesia gibsoni cDNA library with acutely infected dog serum. The BgP27 is a single copy gene with a predicted open reading frame of 762 bp and 254 amino acids. The phylogenic analysis of the deduced amino acid of BgP27 demonstrated considerable identities with members of Plasmodium berghei circumsporozoite protein family that ranged between 18.4% and 22.8%. The BgP27 was expressed as a glutathione S-transferase fusion protein in Escherichia coli. The serum raised in mice against the recombinant protein specifically reacted with a 27-kDa protein in the extracts of B. gibsoni parasites. Confocal laser scanning microscopic observation showed high fluorescent reactivity with both...
Palavras-chave: Babesia gibsoni; Enzyme-linked immunosorbent assay; Deiagnostic performance.
Ano: 2008
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2232

Development of Monoclonal Antibodies That Target 1-Cys Peroxiredoxin and Differentiate Plasmodium falciparum from P. vivax and P. knowlesi
Provedor de dados: 19
Autores: Hakimi, Hassan; Nguyen, Thu-Thuy; Suganuma, Keisuke; Masuda-Suganuma, Hirono; Ma. M. Angeles, Jose; Inoue, Noboru; Kawazu, Shin-ichiro; 井上, 昇; 河津, 信一郎.
Prompt and accurate diagnosis of malarial patients is a crucial factor in controlling the morbidity and mortality of the disease. Effective treatment decisions require a correct diagnosis among mixed-species malarial patients. Differential diagnosis is particularly important in cases of Plasmodium vivax, a species that shares endemicity with P. falciparum in most endemic areas. Moreover, it is difficult to identify P. knowlesi on the basis of morphology alone, and rapid diagnostic tests are still not available for this malaria species. Therefore, the development of diagnostic tests applicable to the field is urgently needed. 1-Cys peroxiredoxin (1-Cys-Prx) in P. falciparum is abundantly expressed in the mature asexual stages, making it a promising...
Palavras-chave: Malaria diagnosis; Monoclonal antibody; Peroxiredoxin; Plasmodium knowlesi; P. vivax.
Ano: 2013
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3859

Rapid and Sensitive Method for Detection of Newly Isolated Babesia Parasite (Babesia sp.1) in the Anticipated Vector-Tick Using the Polymerase Chain Reaction Technique
Provedor de dados: 19
Autores: Ohta, Masato; Kawazu, Shin-ichiro; Tsuji, Naotoshi; Terada, Yutaka; Kamio, Tsugihiko; Fujisaki, Kozo.
Palavras-chave: Babesia ovata; Polymerase chain reaction (PCR); Nested-PCR; Haemaphysalis longicoronis.
Ano: 1995
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/231

Development of DNA Probes for the Detection and Identification of Babesia ovata
Provedor de dados: 19
Autores: Kawazu, Shin-ichiro; Panchadcharam, Chandrawathani; Kawazu, Takeshi; Sekizaki, Tsutomu; Fujisaki, Kozo.
Palavras-chave: DNA probe diagnosis; Babesia ovata.
Ano: 1993
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/187

ウシ小型ピロプラズマ病血清診断用抗原の開発
Provedor de dados: 19
Autores: 河津, 信一郎; Kawazu, Shin-ichiro.
Palavras-chave: ウシ; 感染症; 原虫.
Ano: 2009
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2607

DNA Probes for Detection of Babesia ovata or the Babesia sp.1, A Newly Isolated Bovine Babesia Parasite in Japan
Provedor de dados: 19
Autores: Ohta, Masato; Kawazu, Shin-ichiro; Tsuji, Naotoshi; Terada, Yutaka; Kamio, Tsugihiko; Fujisaki, Kozo.
Palavras-chave: Babesia ovata; DNA probe.
Ano: 1995
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/226

Prevalence of gastrointestinal helminth parasites of zoonotic significance in dogs and cats in lower Northern Thailand
Provedor de dados: 19
Autores: Pumidonming, Wilawan; Salman, Doaa; Gronsang, Dulyatad; Abdelbaset, E. Abdelbaset; Sangkaeo, Khamphon; Kawazu, Shin-ichiro; Igarashi, Makoto.
Gastrointestinal zoonotic helminths of dogs and cats have a public health concern worldwide. We investigated the prevalence of gastrointestinal helminths of zoonotic significance in dogs and cats in lower Northern Thailand and utilized molecular tools for species identification of hookworms and Opisthorchis viverrini. Fecal samples of 197 dogs and 180 cats were collected. Overall prevalence of infection using microscopy was 40.1% in dogs and 33.9% in cats. Helminth infection found in both dogs and cats included hookworms, Spirometra spp., Taenia spp., Toxocara spp., 0. viverrini, Strongyloides spp. and Trichuris spp. Hookworms were the most common helminth in dogs, while Spirometra spp. were the most prevalent in cats. Among hookworm infection in dogs and...
Palavras-chave: Cat; Dog; Gastrointestinal helminth; Thailand; Zoonotic parasite.
Ano: 2016
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4452