Consulta: creatorFacets:"Kim, S"
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Data/hora: 08/06/2024 12:36:20

Microbial extracellular enzyme detection on agar plates by means of fluorogenic methylum-belliferyl-substrates.
Provedor de dados: 5
Autores: Kim, S; Hoppe, H.
A rapid and sensitive method to detect the extracellular enzymatic activity of bacteria colonies grown on agar plates is described. Selective agar media supplemented with protein, starch, chitin, Tween-80, etc. are conventionally used to detect biochemical properties of bacteria. It has been experimentally demonstrated with bacteria pure cultures that fluorogenic Methylumbelliferyl (MUF)-substrates are excellent substrate analogues for normally occurring polymers. Based on MUF-substrate hydrolysis the new method provides reliable qualitative estimates of extracellular enzymatic properties of bacteria within minutes using pure cultures as well as agar plates prepared for colony counts.
Tipo: Text
Palavras-chave: Bacteria; Microbiological culture; Biochemical analysis; Enzymatic activity; Analytical techniques.
Ano: 1984
URL: http://archimer.ifremer.fr/doc/1984/acte-959.pdf