Consulta: creatorFacets:"Kimata, Isao"
Registros recuperados: 2
Data/hora: 07/06/2024 02:21:22

Construction of Polymerase Chain Reaction Primer to Detect Cryptosporidium parvum or C. muris
Provedor de dados: 19
Autores: Zhiliang, Wu; Iseki, Motohiro; Kimata, Isao; Dong, Xu; Nagano, Isao; Takahashi, Yuzo.
Palavras-chave: Cryptosporidium parvum; Cryptosporidium muris; PCR; Primer.
Ano: 1995
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/236

Development and evaluation of an enzyme-linked immunosorbent assay using recombinant p23 for the detection of antibodies to Cryptosporidium parvum in cattle
Provedor de dados: 19
Autores: Bannai, Hiroshi; Nishikawa, Yoshifumi; Seo, Jin-yong; Nakamura, Chinatsu; Zhang, Sofa; Kimata, Isao; Takashima, Yasuhiro; Li, Junyou; Igarashi, Ikuo; Xuan, Xuenan; 西川, 義文; 五十嵐, 郁男; 玄, 学南.
An enzyme-linked immunosorbent assay (ELISA) based on the recombinant p23 of Cryptosporidium parvum was established for the detection of antibodies against C. parvum in cattle. The sensitivity and specificity of the ELISA were evaluated with the standard indirect fluorescent antibody test (IFAT) using sporozoites as antigens. Of 77 bovine sera collected from China, 20 (26.0%) were identified as positive by the IFAT. The same samples were tested with the ELISA. The optical densities at 415 nm were compared to the IFAT results and statistically analyzed to designate a provisional cut-off point. As a result, the cut-off point was concluded to be 0.08, which was considered to be the best condition in the light of its sensitivity (80%) and specificity (73.7%)....
Palavras-chave: Cryptosporidium parvum; P23; ELISA; IFAT.
Ano: 2006
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/142