Consulta: creatorFacets:"Kuznar,Juan"
Registros recuperados: 2
Data/hora: 13/06/2024 10:32:58

Chilean IPNV isolates: Robustness analysis of PCR detection
Provedor de dados: 69
Autores: Jorquera,Esteban; Morales,Paz; Tapia,David; Torres,Pamela; Eissler,Yoanna; Espinoza,Juan C; Conejeros,Pablo; Kuznar,Juan.
Background: The genomes of several infectious pancreatic necrosis viruses (IPNVs) isolated in Chile were sequenced with a single amplification approach for both segments A and B. The resulting sequences were then used to determine the conservation of the primer-binding regions used in polymerase chain reaction (PCR)-based diagnostic methods proposed in the literature. Thus, the robustness of each technique was studied, particularly the eventual effect of further mutations within the primer-binding sites. Results: On analysis, most methods currently used to detect Chilean IPNV varieties were deemed adequate. However, the primers were designed to be genogroup specific, implying that most detection methods pose some risk of detecting all strains prevalent in...
Tipo: Journal article
Palavras-chave: IPNV detection; Mismatch's Tm analysis; QPCR.
Ano: 2016
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000200005

Infectious pancreatic necrosis virus (IPNV) enumeration through epifluorescence microscopy: technical aspects
Provedor de dados: 69
Autores: Pavlov,Maria Soledad; Eissler,Yoanna; Espinoza,Juan Carlos; Garland,David E; Kuznar,Juan.
A method for counting Infectious pancreatic necrosis virus (IPNV) through epifluorescence microscopy was analyzed in detail. Image processing and statistic considerations are included. The particle size of viruses was compared in different experimental conditions such as the staining of the virus with SYBR-Green I or with antibodies for specific fluorescence labeling of viral proteins. The type of surface used as mounting support was assayed as well. The results indicated that the most suitable method involves the mounting of the viral-containing suspension on a membrane filter followed by the staining with a monoclonal antibody specific for a viral protein combined with a FITC (fluorescein isothiocyanate)-conjugated secondary antibody.
Tipo: Journal article
Palavras-chave: Digital image analysis; Fluorescence microscope methods; Immunofluorescence; Infectious pancreatic necrosis virus (IPNV); SYBR-Green I.
Ano: 2011
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000100011