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Characterization of Calreticulin Expression in Mouse Endometrium during Embryo Implantation
Provedor de dados: 48
Autores: CHENG,SHU-QUN; HE,JUN-LIN; DONG,YAN-LING; LIU,XUE-QING; DING,YU-BIN; GAO,RU-FEI; TAN,YI; YE,QIAN; TIAN,ZHEN-LING; WANG,YING-XIONG.
Calreticulin (CRT), a Ca2+-binding storage protein and chaperone in the endoplasmic reticulum, modulates cell adhesiveness and integrin-dependent Ca2+ signaling. However, the role of CRT during implantation remains poorly understood. In the present study, we characterized the expression of CRT mRNA and the protein in mouse endometria from pregnancy DI to D7. Real-Time PCR and in situ hybridization results showed that the levels of CRT mRNA in the endometria of pregnant mice were significantly higher than those of non-pregnant mice (P&lt;0.05), and increased gradually from pregnancy DI to D4, reaching the máximum level on D4, followed by a plateau from D4 to D7. Using immunofluorescence histochemistry and western blot, changes of CRT expression in the...
Tipo: Journal article
Palavras-chave: Calreticulin; Endometrium; Embryo implantation; Mouse.
Ano: 2009
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000400012