Consulta: creatorFacets:"Lambais,Marcio R."
Registros recuperados: 4
Data/hora: 06/06/2024 04:35:31

In silico characterization and expression analyses of sugarcane putative sucrose non-fermenting-1 (SNF1) related kinases
Provedor de dados: 74
Autores: Carraro,Dirce Maria; Lambais,Marcio R.; Carrer,Helaine.
Sucrose non-fermenting-1-related protein kinases (SnRKs) may play a major role in regulating gene expression in plant cells. This family of regulatory proteins is represented by sucrose non-fermenting-1 (SNF1) protein kinase in Saccharomyces cerevisiae, AMP-activated protein kinases (AMPKs) in mammals and SnRKs in higher plants. The SnRK family has been reorganized into three subfamilies according to the evolutionary relationships of their amino acid sequences. Members of the SnRK subfamily have been identified in several plants. There is evidence that they are involved in the nutritional and/or environmental stress response, although their roles are not yet well understood. We have identified at least 22 sugarcane expressed sequence tag (EST) contigs...
Tipo: Info:eu-repo/semantics/article
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572001000100006

In silico differential display of defense-related expressed sequence tags from sugarcane tissues infected with diazotrophic endophytes
Provedor de dados: 74
Autores: Lambais,Marcio R.
The expression patterns of 277 sugarcane expressed sequence tags (EST)-contigs encoding putative defense-related (DR) proteins were evaluated using the Sugarcane EST database. The DR proteins evaluated included chitinases, beta-1,3-glucanases, phenylalanine ammonia-lyases, chalcone synthases, chalcone isomerases, isoflavone reductases, hydroxyproline-rich glycoproteins, proline-rich glycoproteins, peroxidases, catalases, superoxide dismutases, WRKY-like transcription factors and proteins involved in cell death control. Putative sugarcane WRKY proteins were compared and their phylogenetic relationships determined. A hierarchical clustering approach was used to identify DR ESTs with similar expression profiles in representative cDNA libraries. To identify DR...
Tipo: Info:eu-repo/semantics/article
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572001000100015

Cloning of putative ureG genes from Glomus intraradices and urease activities in tobacco arbuscular mycorrhizal roots
Provedor de dados: 63
Autores: Takahashi,Daniele; Ditt,Renata Fava; Lambais,Marcio R.
Even though the major benefit of arbuscular mycorrhizae is the increased uptake of phosphate from the soil solution and translocation to the plant, changes in the activity of enzymes involved in nitrogen (N) metabolism have been detected in mycorrhizal roots. Using differential display of reverse-transcripts of tobacco roots not-inoculated or inoculated with Glomus intraradices (Gi), we have cloned two partial cDNAs (NtGi2 and NtGi3). The presence of a conserved CobW/HypB/UreG domain and phylogenetic analyses suggest that NtGi2 and NtGi3 encode isoforms of urease accessory protein G (ureG) highly similar to ureG from fungi. The steady state levels of the putative ureG transcripts were shown to be higher in roots colonized by Gi, as compared to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nicotiana tabacum; DDRT-PCR; Arbuscular mycorrhiza; Urea; Ammonium sulfate; Nitrogen.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162009000200016

Impact of Amazon land use on the community of soil fungi
Provedor de dados: 63
Autores: Fracetto,Giselle G. M.; Azevedo,Lucas C.B.; Fracetto,Felipe J. C.; Andreote,Fernando D.; Lambais,Marcio R.; Pfenning,Ludwig H.
Considered as one of the most biodiverse biomes, the Amazon has a featured role in the discovery of new species of plants, animals and microorganisms, which may be important for the functionality of different ecosystems. However, studies on the impacts resulted from changes in the Amazon land use on microbial communities and their functions are still limited. In this context, the soil fungal diversity can act as an important indicator of environmental stress caused by land use of the Amazon. This study describes changes in soil fungal communities caused by different systems of land use (primary forest, secondary forest, agroforestry, agriculture and pasture). Communities were observed in each of the areas using denaturing gradient gel electrophoresis...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: DGGE; 18S rRNA; Fungal diversity; Soil biology.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162013000200001